• 한국수정란이식학회지
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• 제24권2호
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• pp.89-95
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• 2009

Successful in vitro embryo production heavily relies on the normal maturation and fertilisation of oocytes. We examined the normal and abnormal fertilisation of zebu cattle oocytes matured in vitro. Immature cumulus oocyte complexes (COCs) from zebu cattle ovaries at slaughter were matured in vitro (IVM) for 24 h. The oocytes were either fixed, stained and examined for nuclear changes or fertilised in vitro (IVF) with Percoll-separated, heparintreated spermatozoa (1.0 ${\times}$ $10^6$/mL) of zebu (n = 7) and crossbred bulls (n = 7). After 18 h of sperm-COCs co-incubation at $39^{\circ}$C with 5% $CO_2$ in humidified air, the presumptive zygotes were fixed, stained and examined for pronuclei. The number of oocytes retrieved per ovary was 5.4 ${\pm}$ 0.7. The percentage of matured oocytes was 73.0. The difference in motility of spermatozoa before and after Percoll seperation was significant (p<0.001). The percentages of normal and abnormal fertilisation (polyspermia and oocytes with one pronucleus) varied significantly depending on individual bulls (p<0.05). A protocol for IVF of IVM oocytes in Bangladeshi zebu cattle is developed. A future study may elucidate the capacity of such IVM-IVF oocytes to develop to the blastocyst stage for transfer to surrogate mother.

• 대한한의학회지
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• 제35권2호
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• pp.1-11
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• 2014

Objectives: To evaluate the effect of acupuncture as an adjunct treatment to increase the success rate of in vitro fertilisation (IVF). Methods: A review of the English and Korean literature was conducted to identify studies on acupuncture as an adjunct treatment to IVF. The main outcome measures were the biochemical pregnancy rate (BPR), the implantation rate (IR), the clinical pregnancy rate (CPR), the miscarriage rate (MR), the on-going pregnancy rate (OPR) and the live birth rate (LBR). Results: Nine meta-analyses of 11 systematic reviews (SRs) were included in this review. In four SRs, the overall IVF outcomes regardless of the procedural steps were documented; two of these SRs reported a significant effect on the CPR. One SR reported that acupuncture at the time of controlled ovarian hyperstimulation had a significant effect on the BPR. Seven SRs reported that acupuncture had no significant impact on transvaginal oocyte retrieval (TVOR). In eight SRs, significant impacts on the CPR, LIBR and OPR were reported when acupuncture was performed around the time of embryo transfer (ET). The results of repeated acupuncture after ET were included in two SRs, which included the same primary studies. The results of the SRs showed that acupuncture had a positive effect on the CPR. Conclusions: When the complete IVF procedure is analysed, there is conflicting evidence in that some reviews have found that acupuncture leads to an increased CPR but others have not. In addition, the reviews presented no evidence to suggest that acupuncture has any specific risks.

• 한국보건행정학회:학술대회논문집
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• 한국보건행정학회 2004년도 전기학술대회
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• pp.129-150
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• 2004

1978년 7월 25일 영국 맨체스터의 올드햄병원에서 세계 최초로 시험관아기 루이스 브라운이 태어나자 생명을 시험관에서 잉태시킬 수 있는가를 놓고 생명윤리에 관한 논쟁이 촉발되었다. 이러한 논쟁은 몇몇 선진국에서는 보조생식술의 이용에 따른 윤리적·사회적· 법적 문제를 논의하기 위한 국가적 차원에서의 조사위원회가 만들어지고 이를 규제하기 위한 법률을 준비하는 계기로 작용하였다. 그러나 현재의 시점에서 볼때 그러한 보조생식기술이 인류에게 큰 불행을 가져온 것으로 생각하는 사람은 많지 않은 듯하다. 세계적으로 십여만명의 아이가 이러한 불임극복 방법을 통하여 태어났으며, 현재 IVF(in Vitro Fertilisation)시술은 불임의 치료방법의 하나로 자리 잡고 있는 것이다. (중략)