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Biological applications of the NanoSuit for electron imaging and X-microanalysis of insulating specimens

  • Ki Woo Kim
    • Applied Microscopy
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    • v.52
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    • pp.4.1-4.11
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    • 2022
  • Field emission scanning electron microscopy (FESEM) is an essential tool for observing surface details of specimens in a high vacuum. A series of specimen procedures precludes the observations of living organisms, resulting in artifacts. To overcome these problems, Takahiko Hariyama and his colleagues proposed the concept of the "nanosuit" later referred to as "NanoSuit", describing a thin polymer layer placed on organisms to protect them in a high vacuum in 2013. The NanoSuit is formed rapidly by (i) electron beam irradiation, (ii) plasma irradiation, (iii) Tween 20 solution immersion, and (iv) surface shield enhancer (SSE) solution immersion. Without chemical fixation and metal coating, the NanoSuit-formed specimens allowed structural preservation and accurate element detection of insulating, wet specimens at high spatial resolution. NanoSuit-formed larvae were able to resume normal growth following FESEM observation. The method has been employed to observe unfixed and uncoated bacteria, multicellular organisms, and paraffin sections. These results suggest that the NanoSuit can be applied to prolong life in vacuo and overcome the limit of dead imaging of electron microscopy.

A study on the visual image of men's suit (남성 정장 수트의 시각적 이미지에 관한 연구)

  • Kim, Jeong-Mee
    • Journal of the Korea Fashion and Costume Design Association
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    • v.23 no.1
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    • pp.113-127
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    • 2021
  • This study aims to analyze the style of men's suits and extract expressional words for the development of semantic differential scales of visual images according to the change in silhouette. Research methods are literature studies, case studies, and questionnaires. The stimuli were created using CLO 3D, a virtual sewing CAD. This study's results are as follows: 1) The British suit jacket has an angled shoulder line using thin pads, and a slightly higher waistline. The pants of the suit have a high waistline and two pleats, so the thighs of the pants are wide. The American suit jacket has a natural shoulder line, and the waistline is not emphasized. The width of the jacket is relaxed, and the length is long. The pants of the suit have a higher waistline, and the pants are wide as well. The Italian suit jacket has wide shoulders, and the waistline is connected lower with a soft curve, and the length is relatively short. The trousers of the suit are tapered and get narrower as it goes down to the hem. 2) Because it originated in the practices of true bespoke tailoring, traditional British suits have a far more 'fitted' silhouette than the mass-produced styles that became emblematic of the American style. By the late 1950s, a sack-style suit was standard American business attire. This technique gives the sack suit its characteristically 'boxy' silhouette. The "Continental" presented a highly tailored silhouette, with padded shoulders, a slim, tight-fitting chest, and a closely tapered waist. The story was that Italian culture valued aesthetics over all else and thus sought the 'slim' suit style as much as possible. 3) The main expressional words of visual images for men's suits differ greatly depending on the silhouette of suit. The visual images are ranked in the order of 'neat', 'classic', 'educated', 'hard', 'mature', for fitted silhouettes. The words of 'masculine', 'basic', 'comfortable', 'simple', 'mature', 'conservative', 'modern', are ranked for boxy silhouettes. And the words of 'slim', 'young', 'neat', are noted for slim silhouettes.

A Study on Consumers\` Preference and Purching Intention of Easy-Order Business Suit (이지오더 신사복에 관한 소비자 선호도 및 구매의사 조사)

  • 천종숙;허지혜;송지영
    • The Research Journal of the Costume Culture
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    • v.9 no.3
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    • pp.389-397
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    • 2001
  • The purpose of this study is to examine the growth potentiality of the Easy-Order System in the domestic male business suit market. This study is based on a questionnaire survey of males, who usually wear a business suit in the work place, and females whose husband wear a business suit. The pertinently answered 108 questionnaires (males: 72 and females: 36) were analyzed with SPSS 8.0. The results of this survey were as follows; 1. Gender, occupation, and height of consumers had a considerable effect on their on their intention of purchasing a preference of this system. 2. Subjects, who were dissatisfied with the limited option of purching a business suit in retail shops, had a significant intention of purchasing a business suit through the Easy-Order System. 3. It had no effect on consumers\\` preference and purching intention of the Easy-Order System whether or not they recognize this system. And the Easy-Order System is not estimated to have major repercussions over consumers by now.

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The Formal Suit Design for Visual Effect of Mid-Aged Men's Shape (Part II) (체형에 적합한 시각효과를 위한 중년남성 정장디자인(제2보))

  • 박순천;이순홍
    • Journal of the Korean Society of Clothing and Textiles
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    • v.27 no.11
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    • pp.1260-1269
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    • 2003
  • This paper endeavors to examine the most suitable formal dress design for mid-aged men who lives in Kwangju, the Republic of Korea. Visual effect in associate design is considered with adjustment, figured and the body type. There are partly similarity differences in associate design visual effect by one's shape and clothes design. Firstly, in thin type, striped figured suit is the most attractive. Single adjustment suit with striped figured tend to be attractive, young and modernistic. Single adjustment without figured is felt the mellowest. Secondly, in standard type, the suit without figured is perceived the most attractive and masculine. The suit with striped figured is looked more young. Single adjustment suit without figured is the most attractive and mellow also single adjustment with figured is felt the youngest. Double adjustment without figured is felt mellow and with striped figured is felt the most modernistic and familiar. Also double adjustment suit with check figured is felt more modernistic. Thirdly, in pyknic type, the solid figured and striped figured of the suits are felt more masculine and single without figured is thought attractive. Single adjustment with check figured is common image. Also, double adjustment with stripe figured is perceived attractive. In conclusion, the adjustment, number of button and figure are very important facts for the clothes for giving whole visual effect of men's suit.

The Formal Wear Design for Visual Effect of Mid-Aged Men's Shape(Part I) (체형에 적합한 시각효과를 위한 중년남성 정장디자인(제1보))

  • 박순천;이순홍
    • Journal of the Korean Society of Clothing and Textiles
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    • v.26 no.11
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    • pp.1547-1557
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    • 2002
  • The purpose of this study is to find the profitable clothes design of middle-aged men by examining visual effects in physical design. The methode of study is experimentation. The sample of this study is people who live in Kwangiu, Korea and 20-50 age's 719. The difference of physical design effect by arrangement, pattern and the shape of body. There are partly resemblance difference in physical design visual effect by the shape of body and clothes design(arrangement and pattern)In weak type, single suit with striped pattern and double suit with check pattern make the upper body look short. Otherwise, double suit with striped pattern have man look tall. In standard type, single arrangement suit with striped pattern make the lower half of body long, so make man look tall. And double arrangement suit with striped pattern have man look tall. The suit without pattern have one's shoulder look wide. In pyknic type, striped pattern make the lower half of one's body look long. But single arrangement with striped pattern have the upper body look big. sing1e suit with striped pattern make lower half of body look long and check pattern make it look short. Totally, arrangement, and pattern are very important clothes due to give an effect on whole visual effect man's suit. Also, these dues were felt differently by each types of body. So, it is confirmed that the type of body is important fluent to make people perceive.

The change in temperature·humidity·perspiration of fire suit when applying phased intensive exercises to fire fighter wearing fire suit (소방공무원의 방화복 착용 후 단계별 운동강도 변화 시 의복 내 온도·습도·발한량 차이)

  • Choi, Seo-Yeon;Park, Il-Gyu;Kong, Il-Chean;Rie, Dong-Ho
    • Journal of the Korea Safety Management & Science
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    • v.15 no.4
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    • pp.97-103
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    • 2013
  • The purpose of this study was to verify the change in temperature humidity perspiration of fire suit when applying phased intensive exercises to fire fighter wearing fire suit. For this study, three male fire fighters took basic physical test and performed 10 minute phased intensive exercises -exercise intensity I (30%VO2max), exercise intensity II (45%VO2max), exercise intensity III (60%VO2max) based on maximum oxygen consumption (VO2max)- wearing fire suit (helmet, boots, air respirator) in treadmill and took a rest. The result of study shows that the temperature in the suit elevated during stabilization period after each exercise intensity, humidity elevated as exercise intensity increased, perspiration elevated as exercise intensity increased. This study indirectly ascertained the fire suit's physiological change in fire fighters during field activities.

The effect of consumers' needs for uniqueness and pursuit of clothing benefit on bespoke suit purchase intention amongst 20~30-year-old males (20~30대 남성 소비자의 독특성 욕구와 의복추구혜택이 비스포크 정장 구매 의도에 미치는 영향)

  • Shin, Kyoung Rok;Lee, Eun-Jung
    • The Research Journal of the Costume Culture
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    • v.25 no.2
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    • pp.192-205
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    • 2017
  • Modern men are much more interested in their appearance than ever before, as well as showing an increased need for uniqueness in order to construct their own sphere and pursue differentiation from others. Besides, changes in life style and the aesthetic sense are causing men to pursue various kinds of clothing benefits so that they want a suit with characteristics beyond the stereotyped design and style. They have started to be actively engaged in clothing purchases, forming a driving force for the growth of the male suit market. Hence, this study has significance in that it offers data conducive to consumers' custom suit purchase by reviewing data on the present condition of the custom suit market and bespoke suits. In addition, it aims to give a summary on the theoretical bases of preceding studies, including the need for uniqueness, pursuit of clothing benefit and concern for the appearance of male consumers, as well as examining the factors that influence on their bespoke suit purchase intentions. Accordingly, this work intends to construct a basic environment for consumers to approach the custom suit market easily and pave the way for male suit markets through offering marketing data and information that is helpful for custom suit-related markets.

Characteristics of a hero in the story the powered suit contents (영웅 서사 속의 강화복 콘텐츠 특성)

  • Sohn, Jong-Nam;Lee, Chang-Jo
    • Journal of Digital Convergence
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    • v.11 no.8
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    • pp.315-320
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    • 2013
  • The content of emerging hero is a material of many roles in the development of video content. In recent years, the hero story taking advantage of the powered suit are appearing, and the popularity is also high. The powered suit was first advocated by Robert Anson Heinlein called science fiction writer, and he contributed to creation of diverse and numerous powered suit content. In particular, the powered suit content combined with epic heroes has become popular with many crush to audiences. In this study, what is the powered suit content and analyzed characteristics within the content of the powered suit by content analysis of utilizing the powered suit materials. It is thought that the powered suit content is gaining popularity to a large audience because it focused on realistic, scientific storyline than a traditional hero narrative.

The Study of Suit Design and Materials for Middle-aged Men′s Visual Effect (중년남성 시각효과를 위한 수트디자인과 소재연구)

  • 박순천
    • Korean Journal of Human Ecology
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    • v.4 no.2
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    • pp.123-140
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    • 2001
  • The purpose of this study is to find the suitable suit design of mid-aged men by examining visual effects in Physical and Psychological side aspects. I used convenience sampling by considering sex and age distribution. These methods were used to analyze data that were frequency analysis, variate analysis, centesimal scale, primary factor analysis, three primary variate analysis, duality variate analysis, equation variate analysis and Cronbach's multiplex range verification 1. Image perception of men's suit was analyzed with arrangement, numbers of button, pattern and the shape of one's body and was divided into charming, youthfulness, modernism. affection and manly. 2. 1) There are partly resemblance difference in physical visual effect by the shape of body and suit design. 2) There are partly resemblance differences in psychological visual effect by one's shape and suit design. In conclusion arrangement, number of button and Pattern are very important to give an effect on whole visual effect man's suit. Also, these were felt differently in each types of body Therefore it is confirmed that the type of body is important fluent to make people perceive.

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Wearing Comfort Evaluation of a Summer Flight Suit to Improve Ventilation (통기성 향상을 위한 하계비행복 설계 및 착용쾌적성 평가)

  • Jeon, Eun-Jin;Park, Sei-Kwon;You, Hee-Cheon;Kim, Hee-Eun
    • Fashion & Textile Research Journal
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    • v.16 no.3
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    • pp.485-491
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    • 2014
  • This study verified the effect of summer flight ventilation developed in a previous study based on wearing comfort evaluation. Seven healthy males in their twenties volunteered for this experiment conducted in aclimatic chamber. The experiment consisted of three consecutive periods of rest (20 minutes), running on a treadmill (10 minutes) and recovery (20 minutes). A comparative evaluation was conducted on the general flight suit which had no ventilation holes and summer flight suit that use subjective satisfaction measures and objective measures. The subjective satisfaction was evaluated according to the criteria of temperature sensation, wet sensation, thermal comfort and fatigue sensation. The objective satisfaction was measured by skin temperature, microclimate (temperature and humidity), sweat rate and thermography. The comparative wearing evaluation identified the summer flight suit decreased the temperature between skin and suit by $0.42^{\circ}C$ (upper arm), $0.9^{\circ}C$ (calf) and the skin temperature by $0.3^{\circ}C$ (shoulder), $0.4^{\circ}C$ (upper arm), $0.5^{\circ}C$ (calf) as compared to the general flight suit. The humidity inside the summer flight suit decreased at head (7.73%), shoulder (5.86%), upper arm (5.26%), and calf (8.73%) compared to the one inside the general flight suit. Thermography showed that the air flowed through ventilation holes (neck and armpit). The design of ventilation holes applied to the summer flight suit can be applicable to overall clothing that requires thermal comfort such as dust-free garments, mechanical clothing and combat uniforms.