• Title/Summary/Keyword: improving reaction

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Increasing the attractiveness of physical education training with the involvement of nanotechnology

  • Jinyan Ge;Yuxin Hong;Rongtian Zeng;Yunbin Li;Mostafa Habibi
    • Advances in concrete construction
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    • v.16 no.6
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    • pp.291-302
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    • 2023
  • As the first part of the body that strikes the ground during running, sports shoes are especially important for improving performance and reducing injuries. The use of new nanotechnology materials in the shoe's sole that can affect the movement angle of the foot and the ground reaction forces during running has not been reported yet. It is important to consider the material of the sole of the shoe since it determines the long-term performance of sports shoes, including their comfort while walking, running, and jumping. Running performance can be improved by polymer foam that provides good support with low energy dissipation (low energy dissipation). Running shoes have a midsole made of ethylene propylene copolymer (EPP) foam. The mechanical properties of EPP foam are, however, low. To improve the mechanical performance of EPP, conventional mineral fillers are commonly used, but these fillers sacrifice energy return. In this study, to improve the magnificence of physical education training with nanotechnology, carbon nanotubes (CNTs) derived from recycled plastics were prepared by catalytic chemical vapor deposition and used as nucleating and reinforcing agents. As a result of the results, the physical, mechanical, and dynamic response properties of EPP foam combined with CNT and zinc oxide nanoparticles were significantly improved. When CNT was added to the nanocomposites with a weight percentage of less than 0.5 wt%, the wear resistance, physical properties, dynamic stiffness, compressive strength, and rebound properties of EPP foams were significantly improved.

Production of ρ-Hydroxyacetophenone by Engineered Escherichia coli Heterologously Expressing 1-(4-Hydroxyphenyl)-Ethanol Dehydrogenase

  • Wenmei Wu;Xiwei Yuan;Xin Gao;Chaoyang Tan;Shunxiang Li;Dehong Xu
    • Journal of Microbiology and Biotechnology
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    • v.34 no.2
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    • pp.467-475
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    • 2024
  • ρ-Hydroxyacetophenone is an important and versatile compound that has been widely used in medicine, cosmetics, new materials, and other fields. At present, there are two ways to obtain ρ-hydroxyacetophenone. One is to extract it from plants, such as Artemisia capillaris Thunb and Cynanchum otophyllum Schneid, and the other is to synthesize it by using chemical methods. Of these two methods, the second is the main one, although it has problems, such as flammable and explosive reagents, difficult separation of by-products, and harsh reaction conditions. To solve these issues, we adopted genetic engineering in this study to construct engineered Escherichia coli containing Hped gene or EbA309 gene. Whole-cell biotransformation was conducted under the same conditions to select the engineered E. coli with the higher activity. Orthogonal tests were conducted to determine the optimal biotransformation condition of the engineered E. coli. The results showed that the optimal condition was as follows: substrate concentration of 40 mmol/l, IPTG concentration of 0.1 mmol/l, an induction temperature of 25℃, and a transformation temperature of 35℃. Under this condition, the effects of transformation time on the ρ-hydroxyacetophenone concentration and cell growth were further studied. We found that as the transformation time extended, the ρ-hydroxyacetophenone concentration showed a gradually increasing trend. However, when the ρ-hydroxyacetophenone concentration increased to 1583.19 ± 44.34 mg/l in 24 h, cell growth was inhibited and then entered a plateau. In this research, we realized the synthesis of ρ-hydroxyacetophenone by biotransformation, and our findings lay a preliminary foundation for further improving and developing this method.

Effects of chloride ion transport characteristics and water pressure on mechanical properties of cemented coal gangue-fly ash backfill

  • Dawei Yin;Zhibin Lu;Zongxu Li;Chun Wang;Xuelong Li;Hao Hu
    • Geomechanics and Engineering
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    • v.38 no.2
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    • pp.125-137
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    • 2024
  • In paste backfill mining, cemented coal gangue-fly ash backfill (CGFB) can effectively utilize coal-based solid waste, such as gangue, to control surface subsidence. However, given the pressurized water accumulation environment in goafs, CGFB is subject to coupling effects from water pressure and chloride ions. Therefore, studying the influence of pressurized water on the chlorine salt erosion of CGFB to ensure green mining safety is important. In this study, CGFB samples were soaked in a chloride salt solution at different pressures (0, 0.5, 1.5, and 3.0 MPa) to investigate the chloride ion transport characteristics, hydration products, micromorphology, pore characteristics, and mechanical properties of CGFB. Water pressure was found to promote chloride ion transfer to the CGFB interior and the material hydration reaction; enhance the internal CGFB pore structure, penetration depth, and chloride ion content; and fill the pores between the material to reduce its porosity. Furthermore, the CGFB peak uniaxial compression strain gradually decreased with increasing soaking pressure, whereas the uniaxial compressive strength first increased and then decreased. The resulting effects on the stability of the CGFB solid-phase hydration products can change the overall CGFB mechanical properties. These findings are significant for further improving the adaptability of CGFB for coal mine engineering.

Optimization of DME Reforming using Steam Plasma (수증기 플라즈마를 이용한 DME 개질의 최적화 방안 연구)

  • Jung, Kyeongsoo;Chae, U-Ri;Chae, Ho Keun;Chung, Myeong-Sug;Lee, Joo-Yeoun
    • Journal of Korea Society of Industrial Information Systems
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    • v.24 no.5
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    • pp.9-16
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    • 2019
  • In today's global energy market, the importance of green energy is emerging. Hydrogen energy is the future clean energy source and one of the pollution-free energy sources. In particular, the fuel cell method using hydrogen enhances the flexibility of renewable energy and enables energy storage and conversion for a long time. Therefore, it is considered to be a solution that can solve environmental problems caused by the use of fossil resources and energy problems caused by exhaustion of resources simultaneously. The purpose of this study is to efficiently produce hydrogen using plasma, and to study the optimization of DME reforming by checking the reforming reaction and yield according to temperature. The research method uses a 2.45 GHz electromagnetic plasma torch to produce hydrogen by reforming DME(Di Methyl Ether), a clean fuel. Gasification analysis was performed under low temperature conditions ($T3=1100^{\circ}C$), low temperature peroxygen conditions ($T3=1100^{\circ}C$), and high temperature conditions ($T3=1376^{\circ}C$). The low temperature gasification analysis showed that methane is generated due to unstable reforming reaction near $1100^{\circ}C$. The low temperature peroxygen gasification analysis showed less hydrogen but more carbon dioxide than the low temperature gasification analysis. Gasification analysis at high temperature indicated that methane was generated from about $1150^{\circ}C$, but it was not generated above $1200^{\circ}C$. In conclusion, the higher the temperature during the reforming reaction, the higher the proportion of hydrogen, but the higher the proportion of CO. However, it was confirmed that the problem of heat loss and reforming occurred due to the structural problem of the gasifier. In future developments, there is a need to reduce incomplete combustion by improving gasifiers to obtain high yields of hydrogen and to reduce the generation of gases such as carbon monoxide and methane. The optimization plan to produce hydrogen by steam plasma reforming of DME proposed in this study is expected to make a meaningful contribution to producing eco-friendly and renewable energy in the future.

Correlations between the Capacity of In Vitro Fertilization and the Assays of Sperm Function and Characteristics in Frozen-thawed Bovine Spermatozoa (소 동결-융해 정자에 있어서 체외수정능력과 정자 기능 및 성상 분석법간의 상관관계)

  • Ryu, B.Y.;Chung, Y.C.;Kim, C.K.;Shin, H.A.;Han, J.H.;Kim, S.H.;Moon, S.Y.;Kim, H.R.;Choi, H.
    • Korean Journal of Animal Reproduction
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    • v.26 no.3
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    • pp.275-289
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    • 2002
  • The objective of this study was to develop an in vitro assessment of sperm fertilizing capacity of bulls and investigate the factors influencing sperm function and characteristics of frozen-thawed bovine spermatozoa. in vitro fertilization (IVF), the evaluation of motility and normal morphology, HOST (hypoosmotic swelling test), Ca-ionophore induced acrosome reaction, luminol and lucigenin-dependent chemiluminescence for the measurement of reactive oxygen species (ROS), the measurement of malondialdehyde formation for the analysis of lipid peroxidation (LPO), and the evaluation of DNA fragmentation using the method of 747-mediated nick end labelling (TUNEL) by flow cytometry were performed in frozen-thawed bovine spermatozoa. Correlations between the rates of fertilization, blastocyst formation after IVF and the values of respective assays were investigated. 1. IVF rate and blastocyst formation rate averaged 64.4% and 34.3% for spermatozoa from high -fertility bull group and averaged 18.5% and 6.2% for spermatozoa from low-fertility bull group, respectively. There were significantly different between two bull groups. Sperm motility and percentage acrosome reaction averaged 79.0% and 66.2% for spermatozoa from high-fertility bull group and averaged 40.7% and 22.9% for spermatozoa from low-fertility bull group, respectivitely. There were not different between two bull groups. 2. Luminol depenent chemiluminescence, LPO and DNA fragementation averaged 6.4, 2.0 nmol and 2.6% from spermatozoa from high-fertility bull group and averaged 6.5, 3.1 nmol and 7.4% for spermatozoa from low-fertility bull group, respectively. There were significantly different between two bull groups. There was no significant difference in lucigenin dependent chemiluminescence between two bull groups. 3. Fertilization rate was positively correlated with motility and the rate of Ca-ionophore induced acrosome reaction, but negatively correlated with the frequency of luminol-dependent chemiluminescence, the rate of LPO, and the percentage of sperm with DNA fragmentation. There was no correlation between fertilization rate and the percentage of swollen spermatozoa, normal morphology, and the frequency of lucigenin-dependent chemiluminescence. 4. Blastocyst formation rate was positively correlated with the rate of Ca-ionophore induced acrosome reaction, but negatively correlated with the frequency of luminol-dependent chemiluminescence, the rate of LPO, and the percentage of sperm with DNA fragmentation. There was no correlation between blastocyst formation rate and motility, the percentage of swollen spermatozoa, normal morphology, and the frequency of lucigenin-dependent chemiluminescence. In conclusion, these data suggest that ROS significantly impact semen quality. The assays of this study may provide a basis fur improving in vitro assessment of sperm fertilizing capacity.

Effects of Mindfulness Based Stress Reduction Program on Depression, Anxiety and Stress in Patients with Aneurysmal Subarachnoid Hemorrhage

  • Joo, Hye-Myung;Lee, Sung-Jae;Chung, Yong-Gu;Shin, Il-Young
    • Journal of Korean Neurosurgical Society
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    • v.47 no.5
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    • pp.345-351
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    • 2010
  • Objective : In this study, the Mindfulness Based Stress Reduction (MBSR) program was applied to patients presenting with depression and anxiety after surgery from spontaneous subarachnoid hemorrhage (SAH) and the effects were assessed. Methods : The subjects were patients admitted for cerebral aneurysm rupture and treated by means of surgery from March to December, 2007. More than 6 months had passed after surgery, without any special lesions showing up on computed tomography (CT), and the Glasgow outcome scale (GOS) was 5 points. Among patients with anxiety and depression symptoms, 11 patients completed the program. The MBSR program was conducted once a week, 2.5 hours each, for 8 weeks. The evaluation criteria were : 1) the Beck Depression Inventory (BDI): it measures the type and level of depression, 2) the State-Trait Anxiety Inventory : the anxiety state of normal adults without mental disorder, and 3) Heart Rate Variability (HRV) : the influence of the autonomous nervous system on the sinoarterial node varies continuously in response to the change of the internal/external environment. Results : The BDI value was decreased from 18.5 ${\pm}$ 10.9 to 9.5 ${\pm}$ 7.1 (p = 0.013) : it was statistically significant, and the depression level of patients was lowered. The state anxiety was decreased from 51.3 ${\pm}$ 13.9 to 42.3 ${\pm}$ 15.2; the trait anxiety was reduced from 50.9 ${\pm}$ 12.3 to 41.3 ${\pm}$ 12.8, and a borderline significant difference was shown (p = 0.091, p = 0.056). In other words, after the treatment, although it was not statistically significant, a decreased tendency in anxiety was shown. In the HRV measurement, standard deviation normal to normal (SDNN), square root of the square root of the mean sum of squared differences between adjacent normal to normal intervals (RMSSD), and total power (TP) showed significant increase, Physical Stress Index (PSI) showed a significant reduction, and thus an improvement in the homeostatic control mechanism of the autonomic nervous system was ween. Conclusion : The MBSR program was applied to the patients showing anxiety and depression reaction after SAH treatment, and a reduction in depression symptoms and physiological reactions were observed. The application of the MBSR program may be considered as a new tool in improving the quality of life for patients after surgery.

Analysis on Inundation Impacts of Sea Level Rise Using System Dynamics-GIS Model (System Dynamics-GIS 모델을 이용한 해수면 상승 침수 영향 분석)

  • KIM, Ji-Sook;KIM, Ho-Yong;LEE, Sung-Ho
    • Journal of the Korean Association of Geographic Information Studies
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    • v.18 no.2
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    • pp.92-104
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    • 2015
  • In order to analyze the impacts of climate change, a time and space integrated model was developed in this study using system dynamics and GIS. The model built was used to carry out a simulation on the inundation impact on A-gu of Busan Metropolitan city resulting from the sea level rise scenario of IPCC and storm surge, which is the worst case. Through this, the flooded area and population until 2100 were predicted. Also, the result and significance of each alternative was reviewed improving the model by establishing alternative scenarios of protection, accommodation and retreat as plans of reaction to sea level rise. The combination of system dynamics and GIS has advantages of how the diverse variables change until the target year can be traced and, accordingly, not only the results but also the processes of spatial change can be examined by calculating the value of change process at each time step. The synergy of this model presumed to be a foothold for solving problems which are becoming difficult to predict due to increase in uncertainty and complexity such as the support for decision making for urban resilience to natural disasters.

A study on the improvement of ability of a creative solving mathematical problem (수학문제의 창의적 해결력 신장에 관한 연구 -농어촌 중학교 수학영재를 중심으로-)

  • 박형빈;서경식
    • Journal of the Korean School Mathematics Society
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    • v.6 no.1
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    • pp.1-17
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    • 2003
  • In this paper, we study the methods of improving an ability of a creative solving mathematical problem belonging to an educational system which every province office of education has adopted for the mathematically talented students. Especially, we give an attention on a preferential reaction in teaching styles according to student's LQ., the relationship between student's LQ. and an ability of creative solving mathematical problems, and seeking for an appropriative teaching methods of the improvement ability of a creative solving problem. As results, we have the followings; 1. The group having excellent students who have a higher intelligential ability prefers inquiry learning which is composed of several sub-groups to a teacher-centered instruction. 2. The correlation coefficient between student's LQ. and an ability creative solving of mathematical is not high. 3. Although the contents and the model of thematic inquiry learning don't have a great influence on the divergent thinking (ex. fluency, flexibility, originality), they affect greatly the convergent thinking - a creative mathematical - problem solving ability. Accordingly, our results show that we should use a variety of mathematical teaching materials apart from our regular textbooks used in schools to improve a creative mathematical problem solving ability in the process of thematic inquiry learning. Also we can see that an inquiry learning which stimulates student's participation and discussion can be a desirable model in the thematic mathematical classroom activities.

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Studies on Cure Kinetics and Thermal Stability of Epoxy/Nylon 6 Blend (에폭시/나일론6 블랜드의 경화 동력학 및 열안정성에 관한 연구)

  • Kim, Dong-Kyu;Kim, Kwan-Woo;Han, Woong;Kwac, Lee-Ku;Kim, Byung-Joo
    • Applied Chemistry for Engineering
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    • v.26 no.5
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    • pp.538-542
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    • 2015
  • In this work, effects of the blend composition composed of 0, 10, 20, 30 and 40 wt% of nylon 6 to epoxy (diglycidylether of bisphenol-A, DGEBA) resin were investigated in terms of cure kinetics and thermal stability by differential scanning calorimeter (DSC) and thermogravimetric analysis (TGA). As the content of the nylon 6 increased, the maximum exothermic temperature ($T_{max}$) and the value of cure activation energy ($E_a$) decreased. The maximum exothermic temperature of the blending samples decreased with increasing in nylon 6 content, resulting in the decrease in curing activation energy of them due to the rapid curing reaction with epoxy resin in this system. From TGA analysis results of the DGEBA/nylon 6, the thermal stability based on the thermal stability index ($A^*{\cdot}K^*$) and integral procedure decomposition temperature (IPDT) increased with increase in the nylon 6 content. This was because of the combination of DGEBA and nylon 6 having good heat resistance, resulting in improving thermal stability of the DGEBA/nylon 6.

Optimal Fixation and Decalcification Methods for Bone Marrow Biopsy (골수생검조직을 위한 최적의 고정 및 탈회 방법)

  • Choi, Myung-Sub;Lee, Hyunsup;Kwon, Hyuk-Chul;Bae, Moon-Hwan;Ko, Young-Hye;Kim, Hee-Jin;Lee, Beom-Se;Koo, Bon-Kyung
    • Korean Journal of Clinical Laboratory Science
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    • v.47 no.4
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    • pp.243-250
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    • 2015
  • A bone marrow biopsy that has undergone decalcification with 10% nitric acid could not be used for various pathological tests and had extremely limited reproducibility. The fixing solution of each experimental group was differentiated in usage, one solution including acid and the other not. The use of the decalcification solution was separated into either acidic or alkaline (EDTA), and further experiments were conducted with differing time phases. When using a fixing solution and decalcification solution which included acid, the specimens were faulty to the extent that all pathological tests were impossible. However specimens that were processed with an EDTA type decalcification solution did not display a non-specific reaction in EBV ISH and were even able to produce results that were at a level suited to various studies or a pathological diagnosis in the FISH, DNA, RNA tests. By improving the fixing and decalcification of bone marrow biopsy, the study was able to make possible ISH, FISH, DNA tests as well as RNA study, and secured the sensitivity, specificity, and reproducibility of various test methods. The stabilization of various test methods that use bone marrow biopsy contributes to the diagnosis, prognosis, prediction, treatment of the patient and provide guidelines for decision-making.