• 대한의생명과학회지
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• 제12권4호
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• pp.419-425
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• 2006

Phospholipase $C-{\gamma}1\;(PLC-{\gamma}1)$ is an important signaling molecule for cell proliferation and differentiation. $PLC-{\gamma}1$ contains two pleckstrin homology (PH) domains, which are responsible for protein-protein interaction and protein-lipid interaction. $PLC-{\gamma}1$ also has two Src homology (SH)2 domains and a SH3 domain, which are responsible for protein- protein interaction. To identity proteins that specifically binds to PH domain of $PLC-{\gamma}1$, we prepared and incubated the glutathione S-transferase(GST)-fused PH domains of $PLC-{\gamma}1$ with COS7 cell lysate. We found that 90 kDa protein specifically binds to PH domain of $PLC-{\gamma}1$. By matrix-assisted laser desorption ionization time of flight-mass spectrometry, the 90 kDa protein revealed to be heat shock protein (Hsp) $90{\beta}$. Hsp $90{\beta}$ is a molecular chaperone that stabilizes and facilitates the folding of proteins that are involved in cell signaling, including receptors for steroids hormones and a variety of protein kinases. To know whether Hsp $90{\beta}$ affects on $PLC-{\gamma}1$ activity, we performed $PIP_2$ hydrolyzing activity of $PLC-{\gamma}1$ in the presence of purified Hsp $90{\beta}$ in vitro. Our results show that the Hsp $90{\beta}$ dose-dependently inhibits the enzymatic activity of $PLC-{\gamma}1$ and further suggest that Hsp $90{\beta}$ regulates cell growth and differentiation via regulation of $PLC-{\gamma}1$ activity.

• 열처리공학회지
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• 제11권4호
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• pp.333-341
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• 1998

The study was investigated on the effect of austenitizing and austempering conditions on retained austenite amount and carbon contents in retained austenite and simultaneously the effect of these variation on hardness, tensile and impact properties. A material of as-cast condition is composed of bull's eye structure with ferrite surrounding spheroidized graphite having about $5-10{\mu}m$ size and matrix structure of pearlite. Then, the contents of spheroidized graphite was about 5%. The retained austenite and carbon contents in the retained austenite were increased with the increasing of austenitizing and austempering temperatures, while the retained austenite showed the peak value and is decreased with increasing of austempering time. With increasing of austenitizing temperature, tensile strength, elongation and impact absorb energy increased and hardness was almost not changed, while with increasing of austempering temperature, tensile strength and hardness decreased, whereas elongation and impact absorb energy was increased. With increasing of retained austenite amount, the tensile strength is slowly decreased but elongation was increased with direct proportion. Also, Impact absorb energy is shown identity value untile about 18%, but rapidly increased above it. Elongation and Impact absorb energy are strongly controlled by the amount of retained austenite, but tensile strength is affected with various factors such as retained austenite amount and bainitic morphology.

• 대한수의학회지
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• 제45권2호
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• pp.155-161
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• 2005

Proteomics is a useful approach to know protein expression, post-translational modification and protein function. We investigated the protein expression pattern and identity in chickens fed with the tissue culture medium waste after harvest of Korean wild ginseng (TCM-KWG) (Panax ginseng). Two groups (n=60/group) of day old broiler chickens were administered with 0 (control) and 0.8% (treatment) TCM-KWG through drinking water. After 5 weeks, we examined the protein expression pattern of fibularis longus and superficial pectoral muscle by Two-dimensional electrophoresis analysis. Interestingly, TCM-KWG treatment significantly increased five spot's density, and markedly reduced five spot's density in the muscles. We identified 10 proteins (desmin, myosin light chain 1, heat shock 25 kDa protein, collapsin response mediator protein-2A, alpha enolase, vimentin, actin alpha 1, my023 protein, pyruvate kinase and troponin T) by the matrix-assisted laser desorption ionization time of flight (MALDI-TOF).

• 한국지능시스템학회논문지
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• 제21권1호
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• pp.6-11
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• 2011

본 논문에서는 고유특징 정규화 및 추출 기법(ERE: Eigenfeature Regularization and Extraction)을 이용한 걸음걸이 바이오 정보 기반 사용자 인식 시스템을 제안한다. 먼저 카메라 센서에서 취득한 걸음걸이 시퀀스로부터 사용자 인식을 위한 특징 정보로 걸음걸이 에너지 영상(GEI: Gait Energy Image)을 생성한다. 학습 단계에서는 갤러리 걸음걸이 에너지 영상에 ERE를 적용하여 정규화된 변환행렬을 획득하여 고유공간(eigenspace)에 사상된 특징정보를 구하고, 검증 단계에서는 걸음걸이 에너지 영상을 학습단계에서 생성한 고유공간에 사상하여 최근접 이웃 분류기를 이용하여 사용자를 인식한다. 제안한 시스템의 유효성 검증을 위해 CASIA 걸음걸이 데이터셋 A를 이용하여 실험하였고, 기존 연구에 비해 인식 정확도 면에서 우수한 성능을 보여주었다.

• 복식문화연구
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• 제27권3호
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• pp.193-205
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• 2019

A trend analysis of research articles in a field of knowledge is significant because it can help in finding out the structural characteristics of the field and the future direction of research through observing change in a time series. We identified the structural characteristics and trends in text data (keywords) gathered from research articles which in itself is an important task in various research areas. The titles and keywords were crawled from research articles published from 2016 to 2018 in the Research Journal of the Costume Culture (RJCC), one of the representative Korean journal in the field of clothing and textile. After we extracted data comprising English titles and keywords from 195 published articles, we transformed it into a 1-mode matrix. We used measures from network analysis (i.e., link, strength, and degree centrality) for evaluating meaningful patterns and trends in the research on clothing and textile. NodeXL was used for visualizing the semantic network. This study observed change in the clothing and textile research trend. In addition to covering the core areas of the field, the subjects of research have been diversifying with every passing year and have evolved onto a developmental direction. The most studied area in articles published by the RJCC was fashion retailing/consumer psychology while aesthetic/historic and fashion industry/policy studies were covered to a more limited extent. We observed that most of the studies reflecting the identity of RJCC share subject keywords to a significant extent.

• Journal of Microbiology and Biotechnology
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• 제33권6호
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• pp.753-759
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• 2023

The genus Paenibacillus contains a variety of biologically active compounds that have potential applications in a range of fields, including medicine, agriculture, and livestock, playing an important role in the health and economy of society. Our study focused on the bacterium SS4^T (KCTC 43402^T = GDMCC 1.3498^T), which was characterized using a polyphasic taxonomic approach. This strain was analyzed using antiSMASH, BAGEL4, and PRISM to predict the secondary metabolites. Lassopeptide clusters were found using all three analysis methods, with the possibility of secretion. Additionally, PRISM found three biosynthetic gene clusters (BGC) and predicted the structure of the product. Genome analysis indicated that glucoamylase is present in SS4^T. 16S rRNA sequence analysis showed that strain SS4^T most closely resembled Paenibacillus marchantiophytorum DSM 29850^T (98.22%), Paenibacillus nebraskensis JJ-59^T (98.19%), and Paenibacillus aceris KCTC 13870^T (98.08%). Analysis of the 16S rRNA gene sequences and Type Strain Genome Server (TYGS) analysis revealed that SS4^T belongs to the genus Paenibacillus based on the results of the phylogenetic analysis. As a result of the matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF/MS) results, SS4^T was determined to belong to the genus Paenibacillus. Comparing P. marchantiophytorum DSM 29850^T with average nucleotide identity (ANI 78.97%) and digital DNA-DNA hybridization (dDDH 23%) revealed values that were all less than the threshold for bacterial species differentiation. The results of this study suggest that strain SS4^T can be classified as a Paenibacillus andongensis species and is a novel member of the genus Paenibacillus.

• 응용통계연구
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• 제37권2호
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• pp.157-176
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• 2024

생명현상의 복잡한 시스템에 대한 이해를 위한 융합분석의 중요성이 점점 커지고 있다. 하나의 연구대상을 다양한 관점에서 관찰하여 얻게 되는 여러 데이터의 융합분석은 통해 좀 더 대상에 대한 깊은 이해를 가능하게 한다. 본 연구에서는 그중에서도 특히 하나의 샘플에서 두개의 고차원 데이터가 생성된 경우 다룰 수 있는 분석인 공관성분석과 정준상관분석을 비교하였다. 정준상관분석의 경우 고차원 데이터를 다룰 수 없는 단점이 있기에, 해당 문제를 극복하기 위하여 능형상수를 이용하는 방법(CCA-ridge)과 각 데이터의 공분산행렬을 항등행렬로 가정하여 벌점화 특이값분해를 이용한 방법(CCA-PMD) 두 가지를 고려하였으며 각 방법을 NCI60 세포주 패널에서 얻은 RNA 시퀀싱 데이터와 단백질 시퀀싱 데이터 분석에 적용하였다. 그 결과 정준상관분석의 경우 두 정준변수간의 상관관계에 좀 더 집중하는 반면 공관성분석은 각 데이터의 선형조합간의 상관관계뿐 아니라 각 선형조합의 변동성을 함께 고려함을 확인할 수 있었다. 또한 공관성분석의 경우 여러가지의 가중치행렬을 고려하여 그 결과값을 비교하고 중요 시사점을 도출하였다.

• ALGAE
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• 제39권2호
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• pp.75-96
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• 2024

Rhodomonas (Cryptophyceae) and species assigned to this genus have undergone numerous taxonomic revisions. This also applies to R. marina studied here as it was originally assigned as a species of Cryptomonas and later considered a variation of R. baltica, the type species. Despite being described more than 130 years ago, R. marina still lacks a comprehensive characterization. Light and electron microscopy were employed to delineate a strain from western Greenland. The living cells were 18 ㎛ long and 9 ㎛ wide, elliptical in shape with a pointed to rounded posterior and truncated anterior in lateral view. Two sub-equal flagella emerged from a vestibulum, where also a furrow extended. In transmission electron microscopy, the furrow was associated with a tubular gullet and the pyrenoid embedded in a deeply lobed chloroplast. The chloroplast contained DNA in perforations and was surrounded by starch grains. A tubular nucleomorph was enclosed within the pyrenoid matrix. In scanning electron microscopy, the inner periplast consisted of rectangular plates with rounded edges and posteriorly these were replaced by a sheet-like structure. The water-soluble pigment was Crypto-Phycoerythrin type I (Cr-PE 545). A phylogenetic inference based on SSU rDNA confirmed the identity of strain S18 as a species of Rhodomonas as it clustered with congeners but also Rhinomonas, Storeatula, and Pyrenomonas. These genera formed a monophyletic clade separated from a diverse assemblage of other cryptophyte genera. To further explore the phylogeny of R. marina a concatenated phylogenetic analysis based on the SSU rDNA-ITS1-5.8S rDNA-ITS2-LSU rDNA region was performed but included only closely related species. The secondary structure of nuclear internal transcribed spacer 2 was predicted and compared to similar structures in related species. Using morphological and molecular signatures as diagnostic features the description of R. marina was emended.

• 생명과학회지
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• 제28권11호
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• pp.1301-1315
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• 2018

참담치(Mytilus coruscus)의 혈구 유래의 항균 펩타이드를 역상 column들을 사용한 reversed-phase high-performance liquid chromatography (RP-HPLC)로 분리 및 정제하였다. 정제된 펩타이드는 matrix-assisted laser desorption ionization time-of-flight mass spectrophotometer (MALDI-TOF/MS)를 통해 분자량이 4041.866 Da으로 밝혀졌으며 Edman degradation법을 통해 25개의 N-말단 서열을 확보하였다. 이는 참담치의 mytilin B precursor와 100%, mytilin 8 precursor, mytilin 4 precursor와 96% 일치하였다. 또한 103개의 아미노산 서열을 코딩하고 있는 312 bp의 open-reading frame (ORF)을 밝혔으며 이는 참담치의 mytilin B precursor와 100% 일치하였다. 밝혀진 분자량과 아미노산 서열을 바탕으로 C-말단 alanine 잔기의 유무에 따라 2개의 펩타이드를 합성하였으며 이는 mytilin B1과 B2라고 명명하였다. 이들은 그람 양성 균주 Bacillus cereus, Streptococcus parauberis [minimal effective concentrations, MECs $41.6-89.7{\mu}g/ml$], 그람 음성 균주 Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Providencia stuartii, Pseudomonas aeruginosa, Vibrio ichthyoenteri [MECs $7.4-39.5{\mu}g/ml$] 그리고 진균류인 Candida albicans [MECs $26.0-31.8{\mu}g/ml$]에 항균활성을 나타냈다. 본 연구 결과, 참담치 혈구 유래 mytilin B1과 mytilin B2는 넓은 항균 스펙트럼을 가지고 열과 염분에 대한 안정성이 높으며 용혈현상과 세포독성은 나타나지 않았다. 이러한 특성은 기능성 사료첨가제 및 항생제 대체제로써 충분히 안정적인 역할을 할 뿐만 아니라 추후 mytilin의 구조적 중요성과 참담치의 면역학적 측면에서 다양한 자료를 제시할 것으로 사료된다.

• IMMUNE NETWORK
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• 제11권3호
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• pp.175-181
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• 2011

Background: CM1 (centrocyte/-blast marker 1) was defined by a mAb against concavabalin-A (ConA) activated PBMC. It is expressed in germinal center of human tonsil and on the surface of activated PBMC as well as cancer cells. Recently, increased productions of pro-inflammatory mediators were detected from activated PBMC by CM1 ligation. Methods: However, there is a limitation to explain the exact role of CM1 on inflammation and its related mechanisms, since the identity of CM1 is still not clarified. In our previous study, we have already confirmed that soluble form of CM1 was produced by Raji. Therefore, we performed Q-TOF analysis after immunoprecipitation of concentrated Raji culture supernatant using anti-CM1 mAbs. Results: As a result, we found that CM1 is identical to enolase-1(ENO1), a glycolytic enzyme, and we confirmed that results by silencing ENO1 using siRNA. It was also confirmed through competition assay between anti-CM1 and anti-ENO1 mAbs. Finally, we investigated the possible role of CM1 in inflammatory response and cancer. The ligation of CM1 on Raji cells with anti-CM1 mAbs induces the extensive production of prostaglandin $E_2(PGE_2)$. In addition, the increased activity of matrix metalloproteinase (MMP)-2/9 was shown in NCI-N87, stomach cancer cell line by CM1 stimulation. Conclusion: CM1 is identical to ENO1 and it might be an important role in the regulation of inflammatory responses.