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Characterization of Denitrifying and Dissimilatory Nitrate Reduction to Ammonium Bacteria Isolated from Mud Crab Culture Environment

  • Hastuti, Yuni Puji;Rusmana, Iman;Nirmala, Kukuh;Affandi, Ridwan;Fatma, Yuli Siti
    • Microbiology and Biotechnology Letters
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    • v.49 no.3
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    • pp.432-439
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    • 2021
  • Microbial community plays important roles in the culture environment of mud crab Scylla serrata. One of the environmental management efforts for the cultivation of S.serrata is by stabilizing microorganisms involved in nitrogen cycle process. The availability of dissolved inorganic nitrogen in its culture environment under a recirculating system closely relates to the nitrogen cycle, which involves both anaerobic and aerobic bacterial activities. Anaerobically, there are two major nitrogen compound degradation processes, i.e., denitrification and dissimilatory nitrate reduction to ammonium (DNRA). This study aimed to identify denitrifying and DNRA bacteria isolated from the recirculating cultivation of S. serrata. The water samples were collected from anaerobic filters called close filter system, which is anaerobically conditioned with the addition of varying physical filter materials in the recirculating mud crab cultures. The results showed that three denitrifying bacterial isolates and seven DNRA bacterial isolates were successfully identified. The phylogenetic analysis based on 16S rRNA gene of the denitrifying bacteria revealed that HIB_7a had the closest similarity to Stenotrophomonas daejeonensis strain MJ03. Meanwhile, DNRA bacterial isolate of HIB_92 showed a 100% similarity to Bacillus sonorensis strain N3, Bacillus vallismortis strain VITS-17, Bacillus tequlensis strain TY5, Geobacillus sp. strain DB24, Bacillus subtilis strain A1, and Bacillus mojavensis strain SSRAI21. This study provides basic information denitrifying and DNRA bacterial isolates identity which might have the potential to be applied as probiotics in aquaculture systems in order to maintain optimal environmental conditions.

Individual Variable Step-Size Subband Affine Projection Algorithm (독립 가변 스텝사이즈 부밴드 인접투사 알고리즘)

  • Choi, Hun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.26 no.3
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    • pp.443-448
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    • 2022
  • This paper presents a subband affine projection algorithm with variable step size to improve convergence performance in adaptive filtering applications with long adaptive filters and highly correlated input signals. The proposed algorithm can obtain fast convergence speed and small steady-state error by using different step sizes for each adaptive sub-filter in the subband structure to which polyphase decomposition and noble identity are applied. The step size derived to minimize the mean square error of the adaptive filter at each update time shows better convergence performance than the existing algorithm using a variable step size. In order to confirm the convergence performance of the proposed algorithm, which is superior to the existing algorithm, computer simulations are performed for mean square deviation(MSD) for AR(1) and AR(2) colored input signals considering the system identification model.

The development of encoded porous silicon nanoparticles and application to forensic purpose (코드화 다공성 실리콘 나노입자의 개발 및 법과학적 응용)

  • Shin, Yeo-Ool;Kang, Sanghyuk;Lee, Joonbae;Paeng, Ki-Jung
    • Analytical Science and Technology
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    • v.22 no.3
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    • pp.247-253
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    • 2009
  • Porous silicon films are electrochemically etched from crystalline silicon wafers in an aqueous solution of hydrofluoric acid(HF). Careful control of etching conditions (current density, etch time, HF concentration) provides films with precise, reproducible physical parameters (morphology, porosity and thickness). The etched pattern could be varied due to (1) current density controls pore size (2) etching time determines depth and (3) complex layered structures can be made using different current profiles (square wave, triangle, sinusoidal etc.). The optical interference spectrum from Fabry-Perot layer has been used for forensic applications, where changes in the optical reflectivity spectrum confirm the identity. We will explore a method of identifying the specific pattern code and can be used for identities of individual code with porous silicon based encoded nanosized smart particles.