• Journal of the Korean Society for Advanced Composite Structures
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• v.5 no.2
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• pp.21-26
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• 2014

This paper introduces general concepts of jointless bridges and field construction case of semi-integral bridge with psc girder integrating end-diaphragm. The expansion joints need to satisfy thermal and safety conditions of bridges. General bridges with joints have some problems, which are frequently replacement cycle time from mechanical damage or unstable movement, maintenance cost and more. To solve these problems, Integral Abutment Bridges(IAB) have been applied overseas in the 1930s. In Korea, first IAB was constructed in the early 2000s and precast IAB systems was invented and applied lately. Kyungshin overpass bridge in Incheon is the Semi-IAB constructed, the span length is 2@35=70m and the width is 13.9m. The original plan was to use general joint bridge but design field changed with expectations for advanced economic estimation and maintenance. This changed method of B.I.B bridge construction provided not only workability, construction cost but also safety improvement at the same time.

• Reproductive and Developmental Biology
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• v.35 no.1
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• pp.1-8
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• 2011

Techniques to evaluate gene expression profiling, such as sufficiently sensitive cDNA microarrays or real-time quantitative PCR, are efficient methods for monitoring human pluripotent stem cell (hESC/iPSC) cultures. However, most of these high-throughput tests have a limited use due to high cost, extended turn-around time, and the involvement of highly specialized technical expertise. Hence, there is an urgency of rapid, cost-effective, robust, yet sensitive method development for routine screening of hESCs/hiPSCs. A critical requirement in hESC/hiPSC cultures is to maintain a uniform undifferentiated state and to determine their differentiation capacity by showing the expression of gene markers representing all three germ layers, including ectoderm, mesoderm, and endoderm. To quantify the modulation of gene expression in hESCs/hiPSC during their propagation, expansion, and differentiation via embryoid body (EB) formation, we developed a simple, rapid, inexpensive, and definitive multimarker, semiquantitative multiplex RT-PCR platform technology. Among the 9 gene primers tested, 5 were pluripotent markers comprising set 1, and 3 lineage-specific markers were combined as set 2, respectively. We found that these 2 sets were not only effective in determining the relative differentiation in hESCs/hiPSCs, but were easily reproducible. In this study, we used the hES/hiPS cell lines to standardize the technique. This multiplex RT-PCR assay is flexible and, by selecting appropriate reporter genes, can be designed for characterization of different hESC/hiPSC lines during routine maintenance and directed differentiation.

• BMB Reports
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• v.51 no.2
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• pp.85-91
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• 2018

Pluripotent stem cell (PSC) variations can cause significant differences in the efficiency of cardiac differentiation. This process is unpredictable, as there is not an adequate indicator at the undifferentiated stage of the PSCs. We compared global gene expression profiles of two PSCs showing significant differences in cardiac differentiation potential. We identified 12 up-regulated genes related to heart development, and we found that 4 genes interacted with multiple genes. Among these genes, Gata6 is the only gene that was significantly induced at the early stage of differentiation of PSCs to cardiomyocytes. Gata6 knock-down in PSCs decreased the efficiency of cardiomyocyte production. In addition, we analyzed 6 mESC lines and 3 iPSC lines and confirmed that a positive correlation exists between Gata6 levels and efficiency of differentiation into cardiomyocytes. In conclusion, Gata6 could be utilized as a biomarker to select the best PSC lines to produce PSC-derived cardiomyocytes for therapeutic purposes.

• Journal of the Korea Concrete Institute
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• v.17 no.2 s.86
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• pp.229-236
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• 2005

To cope with increasing requirements of cost reduction, labor saving, and rapid construction, the purpose of this study is to investigate the structural behavior of PSC monolithic and spliced girders. Three tests were conducted on reduced-scale girder specimens having same type. This paper presents the result of experimental studies on the load-deflection behavior in which different joint and amount of tendon as major variables were investigated. The first one used a monolithic girder was arranged with three tendons. The second one used a spliced girder was joined with three tendons after producing five segments. And the third girder was produced in same conditions with the second girder and arranged with additional tendons. The experimental results show the difference of behavior between monolithic and spliced girders.

• Journal of the Korea institute for structural maintenance and inspection
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• v.11 no.5
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• pp.99-106
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• 2007

To satisfy with the increased requirements of cost reduction, labor saving, and rapid construction, the purpose of this study is to investigate the structural behavior of PSC monolithic and spliced girders. Three tests were conducted on small-scale girder specimens. This paper presents the result of experimental studies in terms of the load-deflection behavior. Different joint type and tendon amount were investigated as major variables. The monolithic girder was arranged with three tendons. The spliced girder consisted of five segments connected by three tendons. In addition, five-segmented girders connected by more than three tendons were built to examine the effect of the tendon amount. The experimental results show the difference of behavior between monolithic and spliced girders. Moreover, nonlinear finite element method analysis was utilized to verify the experimental result.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2006.04a
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• pp.725-732
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• 2006

This study has been started for the development of a refined live load distribution formula that has safety and precision toward I type prestressed concrete girder bridge. This type of bridge is mainly applied to short span bridges that are $25{\sim}40m$ in length. Based on various structure analysis models that are currently being applied as preceding studies for the development of live load distribution method. an analysis of flexural stiffness ratio for barrier and diaphragm has been performed. As the result of parametric analysis for the changes in flexural stiffness ratio, the effect of barrier on load distribution showed as insignificant in all structural analysis models while analyzing the deflection distribution. Also. the deflection distribution of the models with stiffness of 25% in which the diaphragm eccentricity is accounted for as same as the models with stiffness of 100% in which the diaphragm eccentricity is unaccounted for. This results are verified through the comparison with a experimental data.

• Progress in Superconductivity
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• v.3 no.2
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• pp.229-234
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• 2002

In this paper, we analyzed the fault current performance in a $high-T_{c}$ superconductor(HTS) which was installed on flux-lock reactor with an external magnetic field coil covering the HTS. In this HTS fault current limiter using flux-lock concepts, the initial limiting current level can be controlled by adjusting the inductance of the coils. Furthermore, the current limiting characteristics of $high-T_{c}$ superconducting FCL can be improved by applying the external magnetic field into the $high-T_{c}$ superconductor. This paper discusses current limiting performance according to the inductance of the coil 1 in two cases with ac magnetic field coil or not and suggests the methods to improve the current limiting factor $P_{limit}$, which is defined as the ratio of the limited current $I_{FCL}$ at the current limiting phase to the prospective short -circuit current $I_{PSC}$.TEX> PSC/.

• Reproductive and Developmental Biology
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• v.38 no.2
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• pp.79-84
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• 2014

MicroRNAs (miRNAs) are approximately 22 nucleotides of small noncoding RNAs that control gene expression at the posttranscriptional level through translational inhibition and destabilization of their target mRNAs. The miRNAs are phylogenetically conserved and have been shown to be instrumental in a wide variety of key biological processes including cell cycle regulation, apoptosis, metabolism, imprinting, and differentiation. Recently, a paper has shown that expression of the miRNA-302/367 cluster expressed abundantly in mouse and human embryonic stem cells (ESCs) can directly reprogram mouse and human somatic cells to induced pluripotent stem cells (iPSCs) efficiently in the absence of any of the four factors, Oct4, Sox2, c-Myc, and Klf4. To apply this efficient method to porcine, we analyzed porcine genomic sequence containing predicted porcine miRNA-302/367 cluster through ENSEMBL database, generated a non-replicative episomal vector system including miRNA-302/367 cluster originated from porcine embryonic fibroblasts (PEF), and tried to make porcine iPSCs by transfection of the miRNA-302/367 cluster. Colonies expressing EGFP and forming compact shape were found, but they were not established as iPSC lines. Our data in this study show that pig miRNA-302/367 cluster could not satisfy requirement of PEF reprogramming conditions for pluripotency. To make pig iPSC lines by miRNA, further studies on the role of miRNAs in pluripotency and new trials of transfection with conventional reprogramming factors are needed.

• Journal of Korean Society of Disaster and Security
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• v.15 no.3
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• pp.67-77
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• 2022

In South Korea, U-type girder development was attempted as a means to increase the length of I-type girder, but due to the large self-weight according to the post-tension method, the application of rail bridges of 30m or less is typical. There are not many examples of application of pre-tension type girder. This study does not limit the post-tension method, but applies the pre-tension method to induce a reduction in self-weight and materials used due to the reduction of the cross-section. In addition, we intend to apply the on-site pre-tensioning method using the internal reaction arm of the U-type girder. The prestressed concrete U-type girder bridge is composed of a concrete deck slab and a composite section. Compared to the PSC I-type, which is an open cross-section because the cross section is closed, structural performance such as resistance and rigidity is improved, the safety of construction is increased during the manufacturing and erection stage, and the height ratio is reduced due to the reduction of its own weight. Therefore, it is possible to secure the aesthetic scenery and economical of the bridge. As a result, it is expected that efficient construction will be possible with high-quality factory-manufactured members and cast-in-place members. In this paper, the introduction of the pre-tension method on-site and the analytical performance verification of the anchoring block for tension are included.

• Journal of the Korean Recycled Construction Resources Institute
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• v.7 no.2
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• pp.151-157
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• 2019

Concrete bridge constructed in metropolitan cities has different superstructure members like slabs and girders, and their carbonation depths vary with different design strengths and local environmental conditions. In this paper, 54 concrete cores were obtained from prestressed concrete girders and the related tests were performed for compressive strength and carbonation depth measurement. Referred to the specified compressive strength of 24MPa for slab and 35MPa for I-type girder, the strengths from cores were evaluated to 82% and 73% of design grade, respectively. For carbonation depth, the slab member showed 30.6mm of average with 32.9% of COV(Coefficient of Variation) and I-type girder showed 16.7~17.0mm with 22.8~33.6 of COV. The I-type girder has much lower carbonation depth and COV compared to slab member, however it has higher COV than column structures.