• International Journal of Stem Cells
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• v.17 no.1
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• pp.38-50
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• 2024

Induced pluripotent stem cell (iPSC) technology has revolutionized various fields, including stem cell research, disease modeling, and regenerative medicine. The evolution of iPSC-based models has transitioned from conventional two-dimensional systems to more physiologically relevant three-dimensional (3D) models such as spheroids and organoids. Nonetheless, there still remain challenges including limitations in creating complex 3D tissue geometry and structures, the emergence of necrotic core in existing 3D models, and limited scalability and reproducibility. 3D bioprinting has emerged as a revolutionary technology that can facilitate the development of complex 3D tissues and organs with high scalability and reproducibility. This innovative approach has the potential to effectively bridge the gap between conventional iPSC models and complex 3D tissues in vivo. This review focuses on current trends and advancements in the bioprinting of iPSCs. Specifically, it covers the fundamental concepts and techniques of bioprinting and bioink design, reviews recent progress in iPSC bioprinting research with a specific focus on bioprinting undifferentiated iPSCs, and concludes by discussing existing limitations and future prospects.

• Proceedings of the Korea Concrete Institute Conference
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• 1999.04a
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• pp.375-380
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• 1999

It is required efficient section shape like bulb-tee girders with high strength concrete to construct long span bridges economically. However, the trpical design method for PSC I-girders include bulb-tees, the concrete girder prestressed only one time at same time. But in this paper, new design method prestress increases as each load-stress stage. The incrementally prestressed concrete (IPC) girders can reduce the required area of grider section and extend span length by additional prestress. So it is able to construct long span bridges economically by using IPC girders.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2005.04a
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• pp.400-407
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• 2005

The reinforcing effect of modified structure of PSC beams is analyzed in this study. The PSC beams are closed by precast half panels embeding PS tendons at the bottom flange of I-bear The stiffness of box structure is larger and the PS force at half panels makes a time-dependent upward camber of superstructures. The superstructure becomes a second composite structure among 3 elements-PSC ben RC slab, PSC Panel. The time-dependent creep and shrinkage effect at PSC Panels and structural behavior is verified considering construction sequences. The optimal range of to-box reinforcing method is surveyed through reliability analysis.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.42 no.1
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• pp.1-9
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• 2022

Semi-integral abutment bridges are a type of integral abutment bridges. These bridges eliminate expansion joints on the structure and can be used in situations not suitable for full-integral abutment bridge. Moreover, Semi-integral bridges have excellent maintenance and can be economically constructed. This study is about precast wall-type blocks at each end which provide lateral support for PSC girder, as well as acting as retaining walls to resist longitudinal movement of semi-integral abutment bridge. The end-diaphragm connection between ended blocks of PSC girders can be achieved by in-suit nonshrinkage concrete. The results show that 3-point experiment of end-diaphragm beam have an acceptable performance which is so better than results of structural design. Moreover, the effects of backfill soil on semi-integral abutment bridge constructed are analyzed the behavior according to the temperature changes.

• Journal of the Korea institute for structural maintenance and inspection
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• v.26 no.6
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• pp.222-229
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• 2022

The extensive use of external prestressing system with PSC-I beam to strengthen reinforced and prestressed concrete members requires the full understanding of the behavior of the strengthened members. The main approach of this research was to experimentally investigate the flexural performance of the PSC-I beam considering external tendon and an equation was developed which indicated the produced incremental stress in the tendon due to applied load. Further, this equation was verified through performing static loading tests on two experimental specimens considering the presence or absence of external tendon. The test results revealed that, the flexural performance enhanced due to the use of external tendon. Moreover, the magnitude of the estimated flexural strength using proposed stress increasing equation almost coincides with the experimental test results. However, it was the evident of the improvement of the flexural performance of the system by reinforcing with the external tendon and also proved that the proposed equation was valid and effective.

• The Korean Journal of Physiology and Pharmacology
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• v.27 no.3
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• pp.267-275
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• 2023

Cardiotoxicity, particularly drug-induced Torsades de Pointes (TdP), is a concern in drug safety assessment. The recent establishment of human induced pluripotent stem cell-derived cardiomyocytes (human iPSC-CMs) has become an attractive human-based platform for predicting cardiotoxicity. Moreover, electrophysiological assessment of multiple cardiac ion channel blocks is emerging as an important parameter to recapitulate proarrhythmic cardiotoxicity. Therefore, we aimed to establish a novel in vitro multiple cardiac ion channel screening-based method using human iPSC-CMs to predict the drug-induced arrhythmogenic risk. To explain the cellular mechanisms underlying the cardiotoxicity of three representative TdP high- (sotalol), intermediate- (chlorpromazine), and low-risk (mexiletine) drugs, and their effects on the cardiac action potential (AP) waveform and voltage-gated ion channels were explored using human iPSC-CMs. In a proof-of-principle experiment, we investigated the effects of cardioactive channel inhibitors on the electrophysiological profile of human iPSC-CMs before evaluating the cardiotoxicity of these drugs. In human iPSC-CMs, sotalol prolonged the AP duration and reduced the total amplitude (TA) via selective inhibition of I_Kr and I_Na currents, which are associated with an increased risk of ventricular tachycardia TdP. In contrast, chlorpromazine did not affect the TA; however, it slightly increased AP duration via balanced inhibition of I_Kr and I_Ca currents. Moreover, mexiletine did not affect the TA, yet slightly reduced the AP duration via dominant inhibition of I_Ca currents, which are associated with a decreased risk of ventricular tachycardia TdP. Based on these results, we suggest that human iPSC-CMs can be extended to other preclinical protocols and can supplement drug safety assessments.

• Journal of the Korea institute for structural maintenance and inspection
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• v.25 no.1
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• pp.67-74
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• 2021

This paper evaluated the structural safety of an aging PSC I-girder bridge with rebar corrosion in the deck. The geometry and rebar of the bridge were designed based on an actual PSC I-girder bridge, and the numerical analysis was performed considering the crack of concrete and yielding of steel rebar. According to the evaluation criteria of Korea Infrastructure Safety and Technology Corporation, this study defined two criteria of rebar exposure and corrosion rates to construct a total of 32 corrosion scenarios. Rebar exposure was defined as the exposure of tensile rebars in the bridge deck due to the removal of cover concrete. The results of the analysis showed that the safety and rating factors of the bridge decreased with increasing rebar exposure and corrosion rates. For the rebar corrosion rate more than 50%, the safety grade of the bridge should be carefully evaluated for all the rebar exposure rate. When the rebar corrosion rate exceeds 57%, the bridge was evaluated as E grade regardless of rebar exposure rate. A correlation analysis for a 2% of rebar exposure rate found that the bridge was evaluated as A grade up to 55.8% corrosion rate, C grade up to 56.9%, D grade up to 58.5%, and E grade for corrosion rate greater than 58.5%. This study indicates the necessity of a quantitative evaluation of rebar corrosion for evaluating the structural safety of aging bridges.

• Proceedings of the Korea Concrete Institute Conference
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• 2002.05a
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• pp.507-512
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• 2002

Prestressed concrete I-girders were used in the bridge applications in the early 1950s. During the last four decades, the most widely used girder length of bridges have been below 30 meters. The main objective of this study is to develope the alternative section for widely spaced girder of 30 meters span bridge. Girder spacing, the number of strands and compressive strength of concrete are major parameters for widely spaced girders. The optimal girder spacing is determined through the parameter studies of design using widely spaced girders. 30m span bridges of widely girder spacing must use high-strength concrete. Although the basic unit cost of concrete is higher for high-strength concrete, it may be partially or even fully offset by reduced quantities of concrete as result of the smaller number of girders used. High-strength concrete girders have more prestressing strands per girder, but the total number of strands for all of the girders is less than that required for the larger number of normal-strength concrete girders. It could design PSC-I Birdge with widely spaced girder owing to high-strength concrete.

• Magazine of the Korea Concrete Institute
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• v.27 no.1
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• pp.45-48
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• 2015

• Computational Structural Engineering
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• v.20 no.1 s.75
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• pp.71-77
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• 2007