• The Korean Journal of Physiology and Pharmacology
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• v.23 no.5
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• pp.393-402
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• 2019

Aurora kinases inhibitors, including ZM447439 (ZM), which suppress cell division, have attracted a great deal of attention as potential novel anti-cancer drugs. Several recent studies have confirmed the anti-cancer effects of ZM in various cancer cell lines. However, there have been no studies regarding the cardiac safety of this agent. We performed several cytotoxicity, invasion and migration assays to examine the anti-cancer effects of ZM. To evaluate the potential effects of ZM on cardiac repolarisation, whole-cell patch-clamp experiments were performed with human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and cells with heterogeneous cardiac ion channel expression. We also conducted a contractility assay with rat ventricular myocytes to determine the effects of ZM on myocardial contraction and/or relaxation. In tests to determine in vitro efficacy, ZM inhibited the proliferation of A549, H1299 (lung cancer), MCF-7 (breast cancer) and HepG2 (hepatoma) cell lines with $IC_{50}$ in the submicromolar range, and attenuated the invasive and metastatic capacity of A549 cells. In cardiac toxicity testing, ZM did not significantly affect $I_{Na}$, $I_{Ks}$ or $I_{K1}$, but decreased $I_{hERG}$ in a dose-dependent manner ($IC_{50}$: $6.53{\mu}M$). In action potential (AP) assay using hiPSC-CMs, ZM did not induce any changes in AP parameters up to $3{\mu}M$, but it at $10{\mu}M$ induced prolongation of AP duration. In summary, ZM showed potent broad-spectrum anti-tumor activity, but relatively low levels of cardiac side effects compared to the effective doses to tumor. Therefore, ZM has a potential to be a candidate as an anti-cancer with low cardiac toxicity.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.28 no.5
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• pp.523-533
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• 2015

A reactor containment acts as a final barrier to prevent leakage of radioactive material due to the possible reactor accidents into external environment. Because of the functional importance of the containment building, the SIT(Structural Integrity Test) for containments shall be performed to evaluate the structural acceptability and demonstrate the quality of construction. An initial numerical analysis was performed to simulate the results obtained from the SIT for a prestressed concrete(PSC) structure. But the analysis results by the initial model expected smaller displacements than the measured ones by 30% at some locations. Accordingly, the research and development to improve the initial model to corelate the measured results of the SIT more properly have been performed. In this paper, the effects of the loss of concrete due to duct for tendons and the contact of duct and tendons in un-bonded tendon system are mainly evaluated based on the preliminary analysis results. In addition, the importances of the proper definition of mesh connectivity among structural elements of concrete, liner plates, rebars and tendons are discussed.

• Molecules and Cells
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• v.44 no.8
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• pp.541-548
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• 2021

The discovery of human pluripotent stem cells (PSCs) at the turn of the century opened the door to a new generation of regenerative medicine research. Among PSCs, the donors available for induced pluripotent stem cells (iPSCs) are greatest, providing a potentially universal cell source for all types of cell therapies including cancer immunotherapies using natural killer (NK cells). Unlike primary NK cells, those prepared from iPSCs can be prepared with a homogeneous quality and are easily modified to exert a desired response to tumor cells. There already exist several protocols to genetically modify and differentiate iPSCs into NK cells, and each has its own advantages with regards to immunotherapies. In this short review, we detail the benefits of using iPSCs in NK cell immunotherapies and discuss the challenges that must be overcome before this approach becomes mainstream in the clinic.

• Journal of Genetic Medicine
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• v.9 no.2
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• pp.67-72
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• 2012

The generation of induced pluripotent stem cells (iPSCs) derived from patients' somatic cells provides a new paradigm for studying human genetic diseases. Human iPSCs which have similar properties of human embryonic stem cells (hESCs) provide a powerful platform to recapitulate the disease-specific cell types by using various differentiation techniques. This promising technology has being realized the possibility to explore pathophysiology of many human genetic diseases at the molecular and cellular levels. Furthermore, disease-specific human iPSCs can also be used for patient-based drug screening and new drug discovery at the stage of the pre-clinical test in vitro. In this review, we summarized the concept and history of cellular reprogramming or iPSC generation and highlight recent progresses for disease modeling using patient-specific iPSCs.

• Proceedings of the Korea Multimedia Society Conference
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• 2004.05a
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• pp.167-170
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• 2004

본 논문에서는 다양한 디지털 컨텐츠들 중에 디지털 비디오에 대한 워터마킹 방법을 제안하였다. 현재 비디오 압축 부호화의 기본 알고리즘으로 사용되는 DCT(discrete cosine transform)는 고압축을 수행할 경우 블록킹 현상이 심하게 나타나는 단점이 있다. 제안한 방법은 이러한 단점을 극복하고 점진적 전송 특성을 갖는 비디오를 위해 웨이브릿 변환 영역에서 SPIHT(set partitioning in hierarchical trees)를 이용한 비디오 부호화 과정에 워터마크를 삽입하였다. SPIHT 부호화 과정에서 비디오 신호의 시간 중복성을 이용한 I 프레임에 대한 이전 프레임의 움직임 벡터를 이용하여 I 프레임에 워터마크를 삽입한다. 워터마크 삽입 시 HVS(human visual system)를 이용하여 움직임 벡터 분석에 의해 선택된 영역에서 지각적 중요 계수 (PSC :perceptual significant coefficient)를 선택하여 워터마크를 삽입한다. 실험 결과 시간 중복성과 HVS를 이용하여 I 프레임에 워터마크를 삽입하였기 때문에 비가시성과 강인성이 우수함을 확인하였다.

• The Journal of the Korea Contents Association
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• v.14 no.7
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• pp.511-517
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• 2014

The superstructure type of the railway bridge in our country, is mainly classified into the box girder and the I-type girder. The box girder is widely used in the high speed railway bridge because of the safety due to dynamic behavior. The I-type girder is used in the conventional railway bridge, and is also divided into the general type and the composite type, and the newly modified types have been developed. According to the current railway bridge design code, the girder design by the span length in various railway bridge types are performed in this study. The suitable girder height by the span length are analyzed, and the comparative analysis of the structural efficiency and the economical efficiency is carried out. From this study, the composite type girder is appeared the good result in respect of the structural efficiency. However, in the economical aspect, the general I-type girder is required less cost than the other types.

• Journal of the Korean Society for Railway
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• v.11 no.5
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• pp.471-476
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• 2008

The superstructure type of the railway bridge in our country, is mainly classified into the box girder and the I-type girder. The box girder is widely used in the high speed railway bridge because of the safety due to dynamic behavior. The I-type girder is used in the conventional railway bridge, and is also divided into the general type and the composite type, and the newly modified types have been developed. According to the current railway bridge design code, the girder design by the span length in various types of railway bridge is performed in is study. The suitable girder height and the construction cost by the span length are analyzed, and the comparative analysis of the structural efficiency and the economical efficiency is carried out. From this study, the composite type girder is appeared the good result in respect of the structural efficiency. However, in the economical aspect, the general I-type girder is required less cost than the other types.

• Journal of the Korea Concrete Institute
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• v.12 no.6
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• pp.43-50
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• 2000

In this paper, a new splicing method was applied to design the girder section of bridges with the span length of 25m, 30m, 35m, 40m and 45m. A U-type precast prestressed section was also determined for each bridge. Additionally, the sectional area, beam depth and Guyon's efficiency factor of the spliced U-type sections in each span were analyzed in comparison with the present I-type PSC bridges. As a result, in spite of an increase of 31%∼50% in the sectional areas compared with the I-type precast girders, the spliced U-type the beam depth of the spliced U-type girder was designed as 2,050 mm compared with the I-type precast girder of 2,600mm in a 40m span bridge. The sectional efficiency factors of the spliced U-type sections were analyzed as 0.76∼0.99. It shows that the spliced U-type sections ar of a superior structural efficiency in contrast to the average sectional efficiency factor of 0.66 value in the I-type girders.

• Journal of Korea Multimedia Society
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• v.10 no.4
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• pp.450-461
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• 2007

In this paper, we propose the method to use power-saving mode (PSM) applicable to non real-time traffic and PSM applicable to real-time traffic simultaneously, for VoIP traffic with silence suppression. The proposed method uses PSC II during talk-spurt interval of parties A and/or B and uses PSC I or probabilistic sleep interval decision (PSID) method during mutual silence interval, respectively. To evaluate the performance of hybrid PSM (HPSM) based on PSC II or PSID method, we present average buffering delay, energy consumption of mobile station and VoIP packet drop probability with simulation runs. Results shows that proposed HPSM decreases energy consumption of mobile station up to 25 % while satisfying the packet drop probability within QoS requirement in case of end-to-end VoIP connection.

• Journal of the Korean Society of Propulsion Engineers
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• v.7 no.3
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• pp.22-29
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• 2003

The propulsion feeding system of KSR-III is composed of tubes, valves and PSC, and controls the flow of propellant entering to engine. The test of PTA-I is carried out to verify the characteristic of propulsion feeding system and component. The tests of operation characteristic of component, hydraulic characteristic of tubes, flow control using venturi, oscillation of dynamic pressure, characteristic of regulator are carried out. Troubles of component are found out, and renewed, and the performance of the propulsion feeding system is verified through PTA-I. The results of PTA-I are used to the configuration of propulsion feeding system and test of PTA-II.