• Biomedical Science Letters
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• v.17 no.3
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• pp.191-196
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• 2011

Noroviruses (noroV) are the major cause of nonbacterial gastroenteritis in humans worldwide. Since noroV cannot yet be cultured in vitro and their diagnosis by electron microscopy requires at least $10^6$ viral particles/g of stool a variety of molecular detection techniques represent an important step towards the detection of noroV. In the present study, we have applied real-time nucleic acid sequence-based amplification (real-time NASBA) for simultaneous detection of NoroV genogroup I (GI) and genogroup II (GII) using standard viral RNA. For real-time NASBA assay which can detected noroV GI and GII, a selective region of the genes encoding the capsid protein was used to design primers and genotype-specific molecular beacon probes. The specificity of the real-time NASBA using newly designed primers and probes were confirmed using standard viral RNA of noroV GI and GII. To determine the sensitivity of this assay, serial 10-fold dilutions of standard viral RNA of noroV GI and GII were used for reverse transcription polymerase chain reaction (RT-PCR) and real-time NASBA. The results showed that while agarose gel electrophoresis could detect RT-PCR products with 10 pg of standard viral RNA, the real-time NASBA assay could detect 100 fg of standard viral RNA. These results suggested that the real-time NASBA assay has much higher sensitivity than conventional RT-PCR assay. This assay was expected that might detect the viral RNA in the specimens which could have been false negative by RT-PCR. There were needed to perform real-time NASBA with clinical specimens for evaluating accurate sensitivity and specificity of this assay.

• Korean Journal of Remote Sensing
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• v.34 no.6_1
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• pp.879-891
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• 2018

In this study, the effects of urban redevelopment and building construction on the change of the detailed flows around the Pukyong National University (PKNU) campus located in the building-congested area was investigated using a CFD (computational fluid dynamics) model and GIS (geographic information system). For the analysis of the detailed flows before and after the constructions of the buildings around and within the campus, numerical simulations for the 16 inflow directions were performed before and after the construction. We used, as reference wind speeds at the inflow boundaries, the averaged wind speeds observed at the Gwangan light beacon (962) where there is no surrounding obstacle (i.e., building and terrain) acting as friction. We analyzed the area fractions in which wind speeds at z = 2.5 m changed after the construction for 16 inflow directions. The area fractions were relatively large in the east-south-easterly and southerly cases, because of the high-rise buildings constructed at the east and the apartment complex and the Engineering buildings constructed at the south of the PKNU campus. In the case of the easterly of which frequency is highest among the wind directions observed at the Daeyeon AWS (AWS 942) located inside the PKNU campus, the wind-speed change was not significant even after the constructions. It is shown that the building construction has affected the detailed flows around as well as even in the far downwind region of the constructed buildings. Also, it is shown that the GIS and CFD model are useful for analyzing the detailed flows in planning the urban redevelopment and/or building construction.