• Journal of Bio-Environment Control
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• v.16 no.1
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• pp.47-53
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• 2007

This study was carried out to identify the treatment methods for the inhibition of lateral roots and promotion of hypocotyl enlargement using plant growth regulators for the production of high quality soybean sprouts. Inhibition of lateral root formation was not significant from the $CA_3$ and NAA treatments. From the treatment of BAP, lateral roots were not occurred at all, and hypocotyl enlargement was promoted at the low concentration of $10\;mg{\cdot}L^{-1}\;and\;15\;mg{\cdot}L^{-1}$. Promotion of hypocotyl enlargement was higher as the BAP treatment time was shorter. The optimum time of BAP treatment was 6 hours. Occurrence ratio of lateral roots were low and hypocotyl enlargement was promoted from water supply every 24 hours after BAP treatment. In the indole B treatment, lateral roots occurrence was very low with the concentration of 1.1%, which is much lower than the optimum concentration of 4.2%.

• Journal of Plant Biology
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• v.39 no.1
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• pp.71-77
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• 1996

Embryogenic callus was selected from callus induced from hypocotyl segment cultures of Daucus carota seedlings on MS basal medium supplemented with 1.0 mg/L 2,4-D. Cell clumps prepared from the embryogenic callus were transferred to MS medium without 2,4-D for somatic embryo development. Cotyledonary abnormalities were frequently observed on somatic embryos developed after the incubation of cell clumps in MS basal medium with 1.0 mg/L 2,4-D for one week and then subculture in the same medium but without 2.4-D for two weeks. The percentage of abnormalities was as follows: 5% one cotyledon, 21% three cotyledons, 6% four cotyledons, 5% five cotyledons, 0.2% six cotyledons and 1% trumpet-like cotyledons. On the other hand, the normal somatic embryo with two cotyledons appeared at 63%. The germination rate of somatic embryos was higher in two cotyledon somatic embryos than in multicotyledonary embryos. Trumpet-like somatic embryos did not germinate normally showing limited elongation and enlargement of roots and cotyledons without shoot development. From anatomical examination circular procambium in the root of somatic embryo began to branch around the middle regions of the hypocotyl which extended into the cotyledons through the cotyledonary nodes and the number of branched procambial strands in hypocotyl was equal to the number of cotyledons. Monocotyledonous somatic embryo always had larger cotyledon than that of somatic embryos with multicotyledons and had horseshoe-like cotyledons where the procambium was of the same structure.ucture.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.3
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• pp.244-249
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• 2002

Varietal difference in seed shape and size, water absorption rate, and soybean sprouts was compared. In general, soybean seeds of yellow seed-coat and spherical shape with 100 seeds of 9.0$\pm$2g were evaluated as the best ones; Pungsannamulkong out of tested varieties was considered to be the best one in these respect. Varietal difference in water absorption yale depending on the soaking duration and temperature was recognized; Jungeri and Jillin 3 showed higher water absorption rate at higher temperature but it was completely vice versa for Pungsannamulkong. It took about 15 hours(soaking at 2$0^{\circ}C$) for seed shape of size to grow to 10mm in length. Maximum enlargement in thickness of soybean seeds was made right after the completion of repeated soaking-drying treatment but three hours and nine hours soaking were needed for one time-soaking and non-soaking treatment, respectively. Varietal difference in germination rate was recognized between one hour's soaking at 2$0^{\circ}C$ and two hour's soaking at 15$^{\circ}C$ The growth rate for Jillin 3 was excellent at all soaking methods and temperatures while three hours of soaking was the best for Jungeril and Pungsannamulkong, regardless of soaking temperatures. The growth of hypocotyl length showed somewhat faster in repeated soaking-drying than one time-soaking and non-soaking while the increase of hypocotyl thicknes was better in one-time soaking than repeated soaking-drying.