• 한국식품영양과학회지
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• 제11권4호
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• pp.7-12
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• 1982

콩나물 재배시(栽培時)에 $25^{\circ}C$에서 blue광(光)을 120Lux로 1일(日) 1회(回) 3시간(時間) 조사(照射)하면서 생장상태(生長狀態), chlorophyll, vitamin C, 단백질함량(蛋白質含量), 단백질(蛋白質)의 pattern 및 trypsin inhibitor activity의 변화(變化)를 암소(暗所)와 상호비교(相互比較)하였다. 콩나물 배축부(胚軸部)의 길이는 blue광(光)에서 다소(多少) 길었으나 함량(重量)에는 차이(差異)가 인정(認定)되지 않았다. Blue광하(光下)에서의 chlorophyll함량(含量)은 3일(日)에 3.57g/100g-f. w., 7일(日)에 8.45g/100g-f. w.로서 재배일정(栽培日數)의 경과에 따라 현저히 증가(增加)하였다. vitamin C의 함량(含量)은 blue광(光) 조사(照射)에 의(依)하여 현저히 증가(增加)되었으며, 재배(栽培) 5일(日)의 자엽부(子葉部)는 21.7% 배축부(胚軸部)는 30.8%가 암소(暗所)에 비(比)하여 높았다. TIA는 blue광(光)에 의(依)한 영향(影響)이 거의 없었으며, 재배(栽培) 5일(日)의 자엽부(子葉部)는 원료대두(原料大豆)와 거의 비슷하였고, 배축부(胚軸部)는 매우 낮아 자엽부(子葉部)의 약(約) 23%에 불과하였다. 단백질(蛋白質)의 함량(含量) 변화(變化)는 blue광(光)의 효과(效果)를 인정(認定)할 수 없었으며, disc electrophoresis를 행(行)한 결과(結果) 원료대두(原料大豆)에서 10개(個), 자엽부(子葉部)에서 9개(個), 배축부(胚軸部)에서 11개(個)의 단백(蛋白)을 분리(分離) 확인(確認)할 수 있었으며 특(特)히 원료대두(原料大豆)와 자엽부(子葉部)에서 존재(存在)가 확인(確認)되지 않은 band No.3이 배축부(胚軸部)의 주요(主要) 구성단백(構成蛋白)임을 알 수 있었다. 그리고 자엽부(子葉部)에서는 blue광(光)의 영향(影響)이 없었으나 배축부(胚軸部)에서는 band No.5와 11이 blue광(光) 조사시(照射時)에서 존재(存在)가 확인(確認)되었다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1994년도 한국생물과학협회 학술발표대회
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• pp.188.1-188
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• 1994

No Abstract, See Full Text

• Journal of Photoscience
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• 제9권2호
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• pp.391-393
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• 2002

Effects of illumination with xenon or sulfur lamp on growth of tomato seedlings were studied. Results showed no obvious differences in net photosynthetic rate and apparent quantum efficiency. In comparison with xenon lamp, illumination with sulfur lamp slightly reduced ChI a and ChI b content in leaves, relatively shortened epicotyl and hypocotyl length, but significantly increased anthocyanin content of leaves and promoted root growth of tomato seedling plants.

• Journal of Plant Biology
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• 제32권4호
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• pp.343-350
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• 1989

Calcium promoted ethylene production from mungbean hypocotyl segments incubated in the presence of either auxin or cytokinin (kinetin). Time course studies indicated that the calcium effect on ethylene production had a longer latent period (about 6 h) in combination with kinetin than with auxin. Studies on the effects of agents that are known to interfere with either action or transport (uptake) of calcium on ethylene biosynthesis indicated different patterns between auxin- and kinetin-treated tissues. Auxin-induced ethylene production was inhibited by the calmodulin inhibitor, trifluoperazine (TFP), and this inhibition was overcome by high concentrations of calcium applied, but TFP had no significant effect on kinetin-induced ethylene production regardless of calcium in the medium. The calcium channel blocker, verapamil, inhibited auxin-induced, but had little effect on kinetin-induced, ethylene producton. In vivo activity of "ethylene forming enzyme (EFE)" was found to be substantially promoted by calcium treatment. The enzyme activity was further increased by kinetin when segments were simultaneously treated with calcium, but auxin did not have such an effect.an effect.

• Journal of Plant Biology
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• 제28권3호
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• pp.233-241
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• 1985

The isolation and culture of protoplasts from hypocotyl-derived calluses of Glycine max (L.) Merr. cv. Jangyeop were obtained by digestion for 6 hrs in an enzyme solution containing 3.5% cellulase, 1.5% macerozyme, 10% sorbitol and 0.1% CaCl2.2H2O at pH 5.8. Newly formed cell wall of protoplasts cultured in MS agar medium containing 10 $\mu$M $\alpha$-naphthaleneacetic acid (NAA) and 32 $\mu$M N6-benzylaminopurine (BAP) could be observed after 24 hrs culture. The first cell division of the protoplasts was observed after 3 days of culture; cell clusters after 2 weeks of culture. When transferred to solid media, the protoplasts formed cell clusters gave rise to proliferating calluses.

• 동아시아식생활학회지
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• 제11권3호
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• pp.219-224
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• 2001

This study was conducted to investigate the optimal conditions for the growth of soybean sprouts by ozone water watering. Optimal conditions for ozone water watering of soybean sprouts during cultivation at 2$0^{\circ}C$ were evaluated with ozone concentration(0.1~O.5 ppm) and watering frequency(1~9 times) by response surface methodology. The optimal conditions for growth of soybean sprouts were ozone concentrations of 0.20~0.32 ppm, ozone treatment frequency of 3.0~4.4 times. Germination rates, hypocotyl weights and hypocotyl lengths in the soybean sprouts cultivated under the optimal conditions increased by 13.3, 10.1 and 11.9%, respectively, whereas root weights decreased by 89.0%.

• Journal of Plant Biotechnology
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• 제6권4호
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• pp.247-252
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• 2004

In vitro cultures of watermelon were treated with antimitotic agent colchicine to induce ploidy alterations, particularly the induction of tetraploids. Explants cotyledon, embryonic end of seed, transverse sections of epicotyl and hypocotyl were cultured on MS media supplemented with BA ($1{\mu}M$) and colchicine ($0.01\%,\;0.05\%\;and\;0.1\%$). Explants were subcultured on colchicine free media after 4 and 7 days. Colchicine had negative effect on in vitro regeneration but this exhibited explants related response. However, hypocotyl section of seedlings induced maximum callus on $0.01\%$ colchicine. Shoot proliferation was more in cotyledon explants cultured on colchicine ($0.01\%$) for four days. Maximum root induction and root number were recorded in embryonic end explants. Overall, cotyledon and embryonic end explants, and low colchicine concentration ($0.01\%$) was found optimal in watermelon regeneration.

• 한국초지학회:학술대회논문집
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• 한국초지조사료학회 1999년도 제24회 정기총회 및 프로그램, 제37회 학술발표회 및 특별강연 초록
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• pp.73.1-73
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• 1999

알팔파의 하배축 (hypocotyl)으로부터 캘러스 유도 및 식물체 재분화를 위하여 2,4-D와 kinetin이 조합 처리된 MS배지에 조직을 치상하였을 때 4주 후 캘러스가 유도되었으며, 2,4-D 4 mg/$\ell$와 kinetin 0.1 mg/$\ell$ 그리고 2,4-D 4 mg/$\ell$와 kinetin 0.5mg/$\ell$ 조합에서 체세포배가 형성되었다 성숙한 체세포배를 MS기본배지로 계대배양하였을때 정상적인 식물체로 재분화 하였다. 이차체세포배 발생을 위하여 재분화된 기내식물의 자엽으로부터 이차캘러스를 유도하였다. 2,4-D의 농도에 따라 배발생캘러스의(중략)

• 생명과학회지
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• 제11권3호
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• pp.254-258
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• 2001

The biochemical change during regeneration of perilla callus were investigated by comparing total protein and lipid contents, protein band pattern in SDS-PAGE, and fatty acid composition in the calli cultured for various period(0, 1, 3, 5 and 6 weeks) Calli were induced from cotyledon and hypocotyl explants of peplants of perilla on perilla on MS medium containing BA(0.5 mg/L) and NAA(0.5mg/L). The protein contents reached the peak at 3 weeks after induction of calli, and then was decreased. Total lipid contents was decreased as the culture period increased. The band pattern of polypeptides showed that 30KD and 45KD polypeptides and 22KD and 45KD polypetides were major proteins in the cotyledon and hypocotyl explants, respectively. However increase of culture period only 30KD protein was highly accumulated.

• 동아시아식생활학회지
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• 제15권6호
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• pp.752-758
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• 2005

This study examined growth and sensory characteristics of soybean sprout cultured at $25\pm1^{\circ}C$ for 4 days with distilled water(control) and green tea extract(0.03 and $0.05\%$). Initial germination rate was higher when soaking in green-tea extract than in the control. The proper concentration of green-tea extract was $0.03\%$. The growth of soybean sprout in $0.05\%$ green-tea extract was better than the control for the hypocotyl thickness and mot hair. Soybean sprout grown with green-tea extract were better than that with control inappearance, flavor, texture and over-all acceptabilitty.