• 한국미생물·생명공학회지
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• 제13권2호
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• pp.145-149
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• 1985

전분자원의 효율적 이용을 위한 방법의 일환으로 분리동정된 전분이용성 효모 H. anomala var. anomala FRI YO-32와 S. cerevisiae와의 세포융합가능성을 검토하기 위하여, 두 효모원형질체의 재생을 위한 최적조건들이 검토되었다. S. cerevisiae에 비하여 FRI YO-32균주의 원형질체가 삼투압안정성이 더 좋았으며 원형질체 재생에 영향을 주는 중요한 인자로서 agar와 삼투압안정제의 농도, 원형 질체 배양방법 등이 검토되었다. 또한 원형 질체 형성을 위한 효소처리 시간이 길어질수록 원형 질체 형성수율은 증가하나 재생효율은 감소하였다.

• 미생물학회지
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• 제24권3호
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• pp.197-203
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• 1986

Streptomyces lavendulae에서 생성된 원형질체 및 원형질체 융합과정에 대한 미세구조 및 행태학적 관찰을 투과 전자현미경과 주사전자현미경을 사용하여 행하였다. 생성된 원행질체들은 고장액에서 대체로 안정하였으나 간혹 변형된 원형질체들이 관찰되었다. 원형질체 융합은 접촉지역 융합지역, 분리지역 형성과정을 차례로 거치면서 선행되는 것이 관찰되었다. 이러한 사실은 세포막 구조의 변화와 이원형의 소실에 의하여 뒷받침이 될 수 있으으로 이러한 변화들을 융합과정에서의 각 단계로 해석하는 것이 가능하리라 사료된다.

• Archives of Pharmacal Research
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• 제10권2호
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• pp.94-99
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• 1987

By using the former equation (8), we modified the equation which can show the dissimilar osmotic behavior of liposome with composition change. The slope of the new equation was presented as the ratio of osmotically active volume (V$_{act}$= ) to the total volume (V$_{totel}$= $_{acl}$+ V$_{dead}$ ; V$_{dead}$ is osmotically inactive volume) of loposomes, we defined is as a Z-value, which can elucidate the dissimilarity of the osmotic activity of multilamellar liposomes with the change of phospholipid composition and the differences of physicochemical properties of liposomes. Z-value was applied for studying the physico-chemical properties of liposomal membrane. The factor that affects on the Z-value was not the lipid concentration of liposome stock dispersion but the lipid composition of liposomal membrane. As the content of dicetylphosphate, the negative charged phospholipid, was increased, the osmotic activity, represented by Z-value, of multilamellar liposome was decreased. Using the hypertonic conditions (shrinking region), Z-value steadily increased and reached a maximum at 10 mole percent cholesterol with increasing the cholesterol content.

• 미생물학회지
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• 제27권4호
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• pp.422-429
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• 1989

Auxotrophic mutant were isolated from wild types by the treatment with NTG as a mutagen, and the conditions of protoplast formation for them were established. The protoplasts of killer yeast Saccharomyces cerevisiae K52 were formed to the level of above 70% when cells grown for 20 hr in PM medium were treated with 200 unit/ml Lyticase 50,000 at $30^{\circ}C$ for 60 min after pretreatment of 50 mM 2-mercaptoethanol in 10mM potassium phosphate buffer (pH 7.5) containing EDTA and 0.6 M sorbitol for 15 min. Also, the protoplast of the recipient S. cerevisiae S 29 were formed to the level of above 85% as it was cultured to the log phase of 24 hr in PM medium under the same conditions. The fusion frequency between the protoplast of killer yeast S. cerevisiae K 52 and the protoplast of recipient S. cerevisiae S 29 was reached to $8.2\times 10^{-6}$ when the hypertonic regeneration medium embeded with the fused protoplasts after mixing the parental protoplasts to 10$^{8}$ cells/ml in SP buffer containing 20 mM $CaCl_{2}$ and 30% PEG 6,000 for 15 min at $30^{\circ}C$ were incubated.

• 한국미생물·생명공학회지
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• 제13권4호
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• pp.345-348
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• 1985

Mannitol hypertonic regeneration media를 사용하는 PEG-induced protoplast transformation system을 이용해서 pUB110과 pE194의 recombinant plasmid로 B. subtilis BR151을 transformation 시킴으로써 두 plasmid에서 유래되는 각각의 Neo$^{R}$와 Em$^{R}$을 동일한 recipient cell 내에서 동시에 발현시킬 수 있었다. Neomycin과 erythromycin을 함께 함유하는 mannitol regeneration media상에서 recombinant plasmid의 transformation frequency는 6.5 $\times$ $10^{-5}$이었다. 한편 transformant cell 내에서 recombinant plasmid의 replication이 agarose gel electrophoresis로 확인되었다.

• 대한의용생체공학회:의공학회지
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• 제29권3호
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• pp.179-186
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• 2008

This paper presents a portable tele-assessment system designed for remote evaluation of the hypertonic elbow joint of neurologically impaired patients. A patient's upper limb was securely strapped to a portable limb-stretching device which is connected through Internet to a portable haptic device by which a clinician remotely moved the patient's elbow joint and felt the resistance from the patient. Elbow flexion angle and joint torques were measured from both master and slave devices and bilaterally fed back to their counterparts. In order to overcome problems associated with the network latency, two different tele-operation schemes were proposed depending on relative speed of tasks compared to the amount of time delay. For slow movement tasks, the bilateral tele-operation was achieved in real-time by designing control architectures after causality analysis. For fast movement tasks, we used a semi-real-time tele-operation scheme which provided the clinicians with stable and transparent feeling. The tele-assessment system was verified experimentally on patients with stroke. The devices were made portable and low cost, which makes it potentially more accessible to patients in remote areas.

• The Korean Journal of Pain
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• 제34권3호
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• pp.346-368
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• 2021

Background: Recalcitrant disc herniation may result in chronic lumbar radiculopathy or sciatica. Fluoroscopically directed epidural injections and other conservative modalities may provide inadequate improvement in some patients. In these cases, percutaneous neurolysis with targeted delivery of medications is often the next step in pain management. Methods: An evidence-based system of methodologic assessment, namely, the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) was used. Multiple databases were searched from 1966 to January 2021. Principles of the best evidence synthesis were incorporated into qualitative evidence synthesis. The primary outcome measure was the proportion of patients with significant pain relief and functional improvement (≥ 50%). Duration of relief was categorized as short-term (< 6 months) and long-term (≥ 6 months). Results: This assessment identified one high-quality randomized controlled trial (RCT) and 5 moderate-quality non-randomized studies with an application of percutaneous neurolysis in disc herniation. Overall, the results were positive, with level II evidence. Conclusions: Based on the present systematic review, with one RCT and 5 non-randomized studies, the evidence level is II for percutaneous neurolysis in managing lumbar disc herniation.

• 대한수의학회지
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• 제30권1호
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• pp.15-27
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• 1990

Effects of oral administration of electrolyte solutions were studied in experimentally dehydrated adult sheep. By the latin square method five ruminal fistulated sheep were examined and dehydrated by deprivation of feed and water for 72 hours. Tap water, physiological saline, 0.45% NaCl+120 mM/L glucose and 0.9% NaCl+1% propylene glycol solution were orally administrated after dehydration, respectively. Rehydration effect and modification of the rumen function were compared. 1. After 72 hours of deprivation of feed and water, sheep were hypertonic dehydrated and blood acid-base parameters were not significantly changed. And there was marked increase in ruminal pH and decrease in ruminal total volatile fatty acid(VFA) concentration. 2. After the fluids administration the changes in blood acid-base parameters were not significant in all groups. 3. Although glucose fermentation in the rumen was observed, 0.45% NaCl+120 mM/L glucose was more effective in rehydration than physiological saline and tap water. But it was difficult to know the rehydration effect of 0.9% NaCl+1% propylene glycol solution exactly because of excessive increase in plasma osmolality. 4. After refeeding, total concentration and proportions of ruminal volatile fatty acid(VFA) were not significantly different among groups and recovered to normal concentration but not in proportions after 2 days in all groups. 5. In vitro cultured ruminal protozoa were susceptible to the decrease of the pH and osmolality.

• Clinical and Experimental Pediatrics
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• 제46권2호
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• pp.192-194
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• 2003

가정분만으로 태어난 후 생후 6일된 신생아에서 전신성 강직-간대 경련이 발생하여 내원한 환아에서, 병력과 진찰 소견상 신생아 파상풍으로 진단하여 근이완제 및 인공환기요법으로 치료한 1례를 경험하였기에 보고하는 바이다.

• The Journal of Korean Physical Therapy
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• 제6권1호
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• pp.171-183
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• 1994

Purphose. This present study examines the effect of brief, intense transcutaneous electrical nerve stimulation(BTENS) on sensory nerve conduction, electrical pain threshold, and two-point discrimination measured at the superficial radial nevre distribution in 20 healthy subjects. Subjects. Twenty volunteercs, (10 females and 10 males(age range : 20-38 years : $mean{\pm}SD\;:\;27.00{\pm}5.12$), only subjects without prior traumatological and pathological were eligible to participated in this study. Methods. Nerve conduction were determined for the right superficial radial nerve. Electrical pain threshold were determined for the right wrist ipsilateral to the site of BTENS. Small disc electrodes were attached to the surface of the skin stradding the end of the radius. Square wave electrical pulses were delivered from an isolated stimulator through a constant current device at a frequency of 2 Hz(5 ms pulse width). Two-point discrimination, measured on the sensory distribution of superficial radial nerve. BTENS was delivered using a Max-SD( Medical design co.) portable battery powered stimulator. A cicular Ag/AgCl electrode in contact with hypertonic saline gel was attached to the lateral(radial side) surface of the forearm. Results. No significant effects were observed between stimulation methods in the prestimulation cycle(multi-way ANOVA repeated measures : distal latency ; F1.14=0.332. amplitude ; F 0.80=0.445, pain threshold ; F0.06=0.940.2 point discrimination ; F1.50=0.236). Highly significant effects were observed time with the pretreatment and 6 posttreatment cycles(p<0.01). Mighty significants differences in nerve conduction and pain threshold were found using un multi-way ANOVA repeated measures among stimulation methods for each cycles(p<0.01). Conclusion and Discussion The authors concludes that both nerve conduction and pain threshold changes are associated with therapy (stimulation) level of BTENS.