• 한국미생물·생명공학회지
• /
• 제17권4호
• /
• pp.327-333
• /
• 1989

미생물에 의한 적자색 색소를 생산하기 위하여 Streptomyces propurpuratus ATCC 21630과 토양에서 분리한 strain No.89를 한천배지상에서 혼합배양하여 적자색을 형성하였다. 적자색 색소를 형성하는 strain No89는 Bacillus sp. R-89로 명명하였다. 이들 두 균주는 각각 배양하였을 때는 색소를 형성하지 않았다. 적자색 색소를 많이 생산하기 위해 Streptomyces Propurpuratus ATCC 21630를 MNNG (N-methyl-N'-nitro-N-nitrosoguanidine) 로 처리하여 얻은 변이주 S.P-6과 Bacillus sp. R-89를 생산배지에서 혼합하여 발효하였을 때 1420$\mu\textrm{g}$/$m\ell$의 색소를 얻을 수 있다.

• 한국미생물·생명공학회지
• /
• 제22권1호
• /
• pp.85-91
• /
• 1994

The characteristcs of monascus fermentation using a hyperpigment-producing mutant, Monascus sp. J101, were analyzed, and the extractive fermentations employing permeabilizing agents and resin were carried out to increase the productivity of red pigment. And the kinetic analysis was also carried out in case of the monascus fermentation using Amberlite XAD-7. The extracellular content of the red pigment produced by Monascus sp. J101 was about 17% of the total, and the production of pigment was regulated by its own product. The cell growth reached a stationary phase at 48 hours ofter inoculation, whereas the pigment production continued up to 100 hours, which showed the pattern of a mixed growth-associated type. During the fermentation, various permeabilizing agents were added to the culture medium and their effects on pigment production were examined. By adding 0.05% Triton X-100 at 48 hours of cultivation, about an 18% increase in pigment production was accomplished as compared to the control, 12% ethyle acetate and 15% for 0.05% deoxycholate, respectively. When a nonionic adsorbent, Amberlite XAD-7 was added to the culture medium at a concentration of 12.0% at 48 hours of cultivation, the pigment production was enhanced by about 48.9% as compared to the control.