• 한국식품조리과학회지
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• 제30권1호
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• pp.76-83
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• 2014

In this study, we have investigated the quality characteristics and antioxidant activities of Rehmannia glutinosa JungKwa (RJ) prepared with different types of sugars (sucrose, isomalto-oligosaccharide, honey or sorbitol). Moisture content was the highest in RJ with honey. The pH level was the highest in RJ with sorbitol, whereas the acidity is the highest in honey. The reducing sugar content is the highest in RJ with honey. The L and a values of Hunter color system showed the highest in RJ with honey, while the b value the highest in RJ with sorbitol. The hardness of texture profile analyses represented the lowest in RJ with sorbitol. Sensory results showed that RJ with sugar was the best in the sensory preference test. The total phenol content was the highest in RJ with honey, while the lowest in RJ with sorbitol. The antioxidant activity determined by $IC_{50}$ values of DPPH and hydroxy radical scavenging activities was the highest in RJ with honey. From these results, it was suggested that RJ with honey show the highest antioxidant activity.

• Asian Pacific Journal of Cancer Prevention
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• 제17권8호
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• pp.3797-3803
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• 2016

Cervical cancer is the second most common malignancy in women worldwide and thus one of the leading causes of mortality in women. Lovastatin, a non polar, anticholesterol drug has previously been reported to exert antitumour activity in vitro. In the present study, lovastatin from Aspergillus terreus (KM017963) was purified by adsoprtion chromatography and evaluated for its anticancer and anti-oxidant properties with a human cervical cancer cell line (HeLa). Growth inhibitory and proapoptotic effects of purified lovastatin on HeLa cells were investigated by determining its influence on cell numbers, mitochondrial membrane potential (MMP), DNA fragmentation and antioxidant properties in terms of hydroxy radical scavenging effects as well as levels of total reduced glutathione. Cell cycle analysis by flow cytometry (propidium iodide staining) confirmed induction of apoptotic cell death and revealed cell cycle arrest in the G0/G1 phase. Results of the study give leads for the anticancer effects of lovastatin and its potential usefulness in the chemotherapy of cervical cancer.

• Natural Product Sciences
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• 제9권4호
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• pp.235-240
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• 2003

Tectorigenin has an apoptosis-inducing ability and immunosuppressive activity. We investigated the effect of tectorigenin on Phase I and II enzyme activities to elucidate the pharmacological action of the immunosuppressive tectorigenin in the diabetic rat. This compound was obtained from the hydrolysis of tetoridin isolated from the flower of Pueraria thumbergiana (Leguminosae). This crude drug (Puerariae Flos) has been used as a therapeutic for diabetes mellitus in traditional Korean medicine. Tecotrigenin inhibited the formation of malondialdehyde (MDA) and hydroxy radicals in serum and liver but promoted superoxide dismutase (SOD) activity. Low MDA contents and low xanthine oxidase and aldehyde oxidase activities were observed in the tectorigenin-treated rats, suggesting that such Phase I enzyme activities are the major source of lipid peroxidation. However, tectorigenin increased Phase II enzyme activities such as SOD, glutathione peroxidase and catalase, suggesting the activation of free radical-scavenging enzymes. The activities of tectorigenin were comparable to those of glibenclamide, which was employed as a positive control. These results suggest that tectorigenin may share some biological properties with glibenclamide in insulin-dependent-diabetes mellitus (IDDM).

• Journal of Animal Science and Technology
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• 제46권6호
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• pp.1007-1012
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• 2004

본 연구에서는 4종류의 Lactobacillus spp.의 항산화 효과와 활성산소에 대한 내성을 측정하였다. 그 결과 L. casei KCTC 3260의 항산화 효과가 intact cell에 서 36.9%, cell lysate에 서 79.8%로서 높은 항산화 효과를 나타내었다. 또 한 lmM 농도의 hydrogen peroxide에 서 생존성에 직접적으로 영향을 받지 않는 등 활성산소에 대해서 높은 내성을 나타내었다. 또한 높은 GPX 활성이 활성산소 하에서 L. casei KCTC 3260의 생존성에 중요한 역할을 하는 것으로 생각된다.

• 한국식품조리과학회지
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• 제31권5호
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• pp.524-533
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• 2015

The purpose of this study was to develop functional bread containing Portulaca oleracea L. (0%, 1%, 3% or 5%). The weight was higher in the bread with Portulaca oleracea L. (POL), compared with the control bread. The height and volume were lower in the bread containing POL. As the concentration of POL increased, the pH decreased and the acidity increased. The sugar concentration ($^{\circ}Brix$) and reducing sugar (%) decreased with increasing amounts of POL. On the hunter color system, L (lightness) and a (redness) values for the crumbs of breads with more POL were lower, but the b (yellowness) value was higher. Total phenolic compound contents increased with the addition of POL. Antioxidant activities such as DPPH and hydroxy radical scavenging activity for the breads with POL increased with increasing POL. The sensory test results showed that the over-all preference score for the breads containing POL were higher than those of the control.

• 한국축산식품학회지
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• 제39권5호
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• pp.725-741
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• 2019

In the current study, we first investigated a method for directly transforming lactose into galacto-oligosaccharides (GOS) for manufacturing low-lactose and GOS-enriched skim milk (GSM) and then evaluated its prebiotic potential by inoculating five strains of Bifidobacterium spp. In addition, fermented GSM (FGSM) was prepared using a potentially probiotic Lactobacillus strain and its fermentation characteristics and antioxidant capacities were determined. We found that GOS in GSM were metabolized by all five Bifidobacterium strains after incubation and promoted their growth. The levels of antioxidant activities including radical scavenging activities and 3-hydroxy-3-methylglutaryl-CoA reductase inhibition rate in GSM were significantly increased by fermentation with the probiotic Lactobacillus strain. Moreover, thirty-nine featured peptides in FGSM was detected. In particular, six peptides derived from ${\beta}$-casein, two peptides originated from ${\alpha}s_1$-casein and ${\kappa}$-casein were newly identified, respectively. Our findings indicate that GSM can potentially be used as a prebiotic substrate and FGSM can potentially prevent oxidative stress during the production of synbiotic fermented milk in the food industry.

• International Journal of Industrial Entomology and Biomaterials
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• 제43권2호
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• pp.45-51
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• 2021

High blood cholesterol levels and oxidized cholesterol are risk factors for cardiovascular disease, which displays high annual incidence. Although studies on sericulture products, including pupae, silk protein, and blood lymph, as hypocholesterolemic substances have been reported, insufficient research in this field has been focused on silkworm larvae. Six larval extracts (Low temperature distilled water, LW; hot temperature distilled water, HW; and 30-100% ethanol, E30-E100) were prepared, and their effects on cholesterol metabolism were examined. LW most potently reduced the risk of cholesterol-related disorders. Polyphenols were highly represented in LW, corresponding with its increased antioxidant potency. The cholesterol biosynthesis enzyme, 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGCR) was strongly inhibited by LW. Hepatocytes over-expressed LDL receptor (LDLR) after LW stimulation, promoting cholesterol elimination from plasma. LW also increased ATP binding cassette transporter 1 (ABCA1) gene expression, upregulating HDL biogenesis. In conclusion, LW exhibited strong antioxidant activity, suppressed cholesterol biosynthesis, improved LDL uptake from plasma, and upregulated HDL biosynthesis. In aggregate, these activities could reduce blood cholesterol levels and prevent cardiovascular disease.

• 한국식품영양과학회지
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• 제40권12호
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• pp.1688-1693
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• 2011

깻잎 부산물(깻잎)의 이용을 증진시키기 위하여 깻잎이 첨가된 기능성 면 대량생산 제조공정을 개발하고, 깻잎 첨가면의 항산화 기능을 유리기 소거능으로 측정하였다. 각 공정별 최적조건 결정은 각 단계별로 제조된 면으로 물 국수를 만들어 외관, 맛, 향, 질감, 기호도에 대해 9점 척도로 관능 평가하였다. 깻잎 데치기 과정은 깻잎 중량 30배의 끓는 물에서 중불로 8분간 깻잎을 데치기 하였을 때 가장 관능평가 결과가 좋았다. 깻잎균질액의 농도는 40%가 가장 적절하였으며 이를 밀가루 중량의 60%(w/w) 첨가하였을 때(최종 농도 19%) 외관, 질감 및 기호도 평가가 다른 조건에 비해 유의적으로 높았다(p<0.05). 이상의 결과에서 만들어진 제조공정에서는 깻잎을 수세하는 과정, 그리고 반죽 시 물을 첨가하는 과정을 단축할 수 있었다. 깻잎첨가면의 DPPH 소거능은 $IC_{50}$ 0.56 mg/mL로 일반면의 11.00 mg/mL에 비해 19.6배 높아졌다(p<0.001). 깻잎첨가면의 superoxide radical 소거능은 $IC_{50}$ 9.53 mg/mL로 일반면의 13.1 mg/mL에 비해 1.4배 높았다(p<0.01). Hydroxyl radical 소거능 역시 $IC_{50}$ 169.2 ${\mu}g$/mL로 일반면의 3 mg/mL에 비해 17.8배 높았다(p<0.001). 이상의 결과로 볼 때 깻잎 첨가에 의해 밀가루면의 관능성과 기능성이 증가되었고, 이는 깻잎에 함유된 페놀화합물 때문으로 생각된다.

• 동의생리병리학회지
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• 제27권5호
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• pp.631-636
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• 2013

This study was to evaluate the antioxidant properties of the leaf extracts of Diospyros lotus (DLE) on the chemical-induced free radical and rat red blood cell (RBC) oxidative damage in vitro. DLE were prepared by extracting with water. DLE showed the high antioxidant activities on the scavenging of 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid)-induced radicals. An antioxidant activities of DLE was similar to the reference antioxidant butylated hydroxytoluene (BHT) and (${\pm}$)6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox). Reducing power of $1,000{\mu}g/mL$ DLE also was similar to the vitamin C. In RBC, oxidative hemolysis induced by the aqueous peroxyl radical generator (2,2'-Azobis (2-methylpropionamidine) dihydrochloride (AAPH)) were significantly suppressed by DLE in a dose-dependent manner. Furthermore, DLE prevented the depletion of cytosolic antioxidant glutathione in RBC damaged with AAPH. These results suggest that DLE may has value as natural product with its high quality antioxidant properties against oxidative stress.

• Applied Biological Chemistry
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• 제46권3호
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• pp.214-219
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• 2003

유색미 19품종에서 염화메틸렌으로 추출한 각 성분들의 항변이원성 및 항산화 효과간의 상관성을 검토하였다. 항변이원성은 $(3.{\beta},22Z)-acetate-stigmasta-5,22-dien-3-ol$, 24-oxocholesterol acetate, 6E,8(E)-heptadiene 및 eicosane의 함량과 정의 상관성이 있었다. 항산화 효과 중 DPPH radical에 대한 전자공여능은 (24R,25S)-aplysterylacetate의 함량과 정의 상관성이 있었고, hydroxy radical 소거활성은 tetradecanoic acid 및 methyl ester-hexadecanoic acid, $(3.{\beta},24S)-stigmast-5-en-3-ol$ 들의 함량과는 각각 부의 상관성이 있었다. 또한 지질과산화 억제효과는 $(3.{\beta},24S)-stigmast-5-en-3-ol$ 함량과는 정의 상관을 그리고 3',3'-dimethylspiro [acridane-9,1'-indane] 및 $(3.{\beta})-24-methylene-9,19-cyclolanostan-3-ol$ 등의 함량과는 부의 상관성이 있었다.