• 제목/요약/키워드: hydroponics culture

검색결과 125건 처리시간 0.024초

오이 캘러스 분화시 광의 영향, 형태발생 및 단백질함량 (Light Influences, Morphogenesis and Protein Content on Callus Differentiation of Cucumber (Cucumis sativus L.))

  • 이은모;조만현;송남현;우인식;이영복;곽상수
    • 식물조직배양학회지
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    • 제27권3호
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    • pp.213-217
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    • 2000
  • 기내에서 무균적으로 생장시킨 오이 유묘의 경단조직과 하배축을 배양하여 캘러스 분화 및 생장에 대해 조사했다. 오이 경단조직을 생장조절물질이 첨가하지 않은 배지에서는 유식 물체로 분화하였으나 2,4-D가 0.5∼2 mg/L 첨가된 MS배지에서는 비배발생캘러스와 점액성캘러스가 유도되었고 배발생 캘러스는 분화되지 않았다. 그러나 유묘 육성시 암조건에서 발아 후 5∼7일 된 개체의 하배축을 5∼10mm크기로 절단하여 2,4-D가 1.0 mg/L이 첨가된 MS배지에 50일간 광조건에서 배양했을 때에는 배발생캘러스와 배상체가 분화되었다. 아울러 하배축 유래 비배발생캘러스 중 백색캘러스는 45.1%가 발근된 반면에, 노랑색캘러스는 불과 0.6%만 발근되었으며. 조단백질의 함량은 백색캘러스보다 노랑색캘러스가 월등히 많았다. 배발생캘러스와 배상체에서 발달된 소식물체는 순화과정을 거쳐 비닐하우스 내의 고형배지경에서 양액재배로 정상적인 과실을 수확하였다.

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Microponic system에서 배양액의 농도변화가 감자 소식물체 생육에 미치는 영향 (The Effect of Nutrient Solution Concentration on Growth of Potato Plantlet in Microponic System)

  • 고선아;최기영;이용범
    • 생물환경조절학회지
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    • 제23권2호
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    • pp.144-147
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    • 2014
  • 감자 가공용 신품종'새봉'의 무병주 대량생산을 위하여 microponic system에서 배양액의 농도변화가 감자 소식물체 생육에 미치는 영향을 구명하고자 수행 하였다. 조절한 감자 배양액의 농도는 EC 0.2, 0.6, 1.0, 1.4, 1.8, $14.0dS{\cdot}^{-1}$수준 이었으며 감자 조직배양묘는 생장점과 잎을 2개 포함한 1.5cm길이로 잘라 50mL 유리병(glass vial)에 1개씩 치상하여 18일, 또는 21일간 2회에 걸쳐 하였다. 배양액 량은 용기 당 2mL씩 넣고 10일 후 1 mL를 첨가하였으며, 환경조건은 일장 16시간, 온도 $23{\pm}1^{\circ}C$, $40mmol^{-2}{\cdot}s^{-1}$의 백색 LED에서 실험 I의 배양액농도는 EC 0.2, 1.0, $14dS{\cdot}m^{-1}$였으며, 실험 II의 배양액농도는 0.6, 1.0, 1.4, $1.8dS{\cdot}m^{-1}$로 조정하였다. 치상 7일 후 소식물체의 생존율은 $0.2dS{\cdot}m^{-1}$에서 90%, $0.6dS{\cdot}m^{-1}$에서 100%, $1.0dS{\cdot}m^{-1}$에서 100%, $1.4dS{\cdot}m^{-1}$에서 0%, $1.8dS{\cdot}m^{-1}$에서 0%, $14.0dS{\cdot}m^{-1}$에서 0% 이었다. 배양액의 전기전도도를 $1.0dS{\cdot}m^{-1}$으로 조절한 처리에서 이식 2일 후 뿌리가 발육되어 소식물체 생육이 왕성하게 생장하여 18일 만에 7개의 엽, 길이 5cm, 생중 0.5g이상의 식물체로 생육하였다. 배양액의 농도를 $0.6dS{\cdot}m^{-1}$으로 조절한 처리에서는 이식 4일 후부터 뿌리가 발육되어 21일 후 5개의 엽, 길이 4cm, 생중 0.2g을 가진 식물체로 생육하였다. 따라서 microponic system에서 무병 감자 묘 대량생산을 위해서는 감자배양액의 전기전도도를 $0.6{\sim}1.0dS{\cdot}m^{-1}$ 조절하여 관리하는 것이 효과적임을 알 수 있었다.

Perlite 단용 및 혼용처리를 이용한 과채류 양액재배 기술 개발 I. 재배용기와 배지의 종류가 양액재배 오이의 생장과 과실품질에 미치는 영향 (Development of Hydroponic Technique of Fruit Vegetables Using Perlite and Mixtures with Perlite as a Substrate I. Effects of Containers and Substrates on the Growth and Fruit Quality of Hydroponically Grown Cucumber)

  • 정순주;서범석;강종구;김홍기
    • 생물환경조절학회지
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    • 제4권2호
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    • pp.159-166
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    • 1995
  • 본 실험은 배수성과 통기성이 우수한 양액 재배 배지로써 perlite와 구입이 용이한 왕겨 및 훈탄의 혼합배지를 이용하여 스티로폼 성형베드 및 상자, 플라스틱 자루, 폿트 등의 재배용기의 차이와 배지의 종류에 따른 양액재배 오이의 생육 및 수량반응을 구명하고자 수행하였 다. 1. 스티로폼베드와 상자가 다른 용기보다 초장이 높게 나타났으며, 스티로폼 상자를 용기로 사용할 때 펄라이트 단용처리구가 275.5cm, 왕겨 혼합처리구가 277.0cm로 초장이 가장 높았다. 2. 엽면적은 스티로폼 성형베드와 상자가 가장 높게 나타났고, 자루재배, 폿트재배의 순으로 나타났다. 스티로폼 성형베드와 상자의 경우에는 펄라이트 단용>왕겨 혼합배지>훈탄 혼합배지의 순으로 나타났으나, 자루재배에서는 왕겨, 폿트재배에서는 왕겨 혼합배지에서 엽면적이 높게 나타났다. 3. 과실수량은 스티로폼 상자를 용기로 한 펄라이트 단용구가 2097.1g으로 가장 많았으며 과수도 13.4개로 높게 나타났다. 4. 상품과율은 스티로폼 베드와 상자에서 70%이상을 나타냈으나 자루재배와 폿트재배에서는 70%이하로 나타났다. 5. 곡과율과 선세과는 자루재배에서 10%이상으로 높게 나타난 반면 스티로폼 상자에서는 낮게 나타났다.

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Establishment of Mass Propagation System of Virus-Free Sweetpotato Plants and Conservation

  • Lee, Joon-Seol;Chung, Mi-Nam;Jeong, Byeong-Choon;Ahn, Young-Sup;Kim, Hag-Sin;Park, Jong-Suk;Bang, Jin-Ki
    • 한국작물학회지
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    • 제52권2호
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    • pp.220-227
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    • 2007
  • Sweetpotato fields in Korea are highly infected with virus and virus like diseases that greatly diminish both yield and quality as indicated by field observations and laboratory tests. In order to solve this problem, there is an urgent need to produce and mass propagate virus-free planting materials for distribution to the farmers. These experiments were conducted, firstly, to determine the most appropriate culture media, nutrient solution, and cutting intervals to maintain growth and vigor of tissue cultured plantleta as mother plants for propagation in insect-proof greenhouse. And as a labor saving method, the production efficiency of plug trays for rapid propagation of stem cuttings as a source of planting materials was likewise evaluated. Results showed that plants grown in medium B supplied with 0.5 and 1.0 strength of MS nutrients had high growth rate, and 20-day cutting interval was the best. 72-plug tray was better than 128-plug. Secondly, it was to develop a technique for the production of first-generation seed roots using hydroponics cultivation system. The yield of virus-free plants propagated in the non-insect proof and open-field cultivation was 2,402 kg/10a, 6% higher than those in the insect-proof cultivation, and the rate of virus re-infection was 18% higher compared to 3.3% with insect-proof cultivation. Lastly, it was to investigate the growth performance of virus free plants in farmers' field. Differences were existed in the yield depending on the variety used, but virus free plants showed an increase of $6{\sim}24%$ over virus infected plants.

토마토 펄라이트 자루재배시 배액전극제어법 적용시점 구명 (Application Time of Irrigation Management by Drainage Level Sensor in Tomato Perlite Bag Culture)

  • 김성은;심상연;김영식
    • 생물환경조절학회지
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    • 제19권1호
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    • pp.19-24
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    • 2010
  • 토마토 펄라이트 자루재배에서 기존에 알려진 배액 전극제어법 적용시기를 가능한 앞당기고, 배양액을 절감하기 위해 수행되었다. 실험처리는 정식후 15일차(T-15), 19일차(T-19), 22일차(T-22)에 배액전극제어법을 적용하는 3개 처리와 타이머법 1개 처리를 실시하였다. 기존의 정식후 1개월 이후에 배액전극제어법 적용하는 관행보다 2주 앞당긴 15일차 처리부터 작물이 배액전극제어법에 반응하는 것으로 나타났다. 생장 및 수확량은 배액전극법에서 높았다. 용수이용효율과 비료이용효율은 T-15 처리에서 가장 경제적이었고, 타이머법 처리는 경제성이 낮았다. 따라서 배액전극제어법 처리시기를 빨리 적용할수록 경제성이 높음을 알 수 있었다.

수경재배 배액 재이용을 위한 주요 곰팡이 및 박테리아 종 파악 (Identification of Major Fungi and Bacterial Species in Solid Medium Drainage for Circulating Hydroponics System)

  • 이동관;손진관;강태경;장재경;박민정;이태석;임류갑
    • 한국환경과학회지
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    • 제29권11호
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    • pp.1109-1123
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    • 2020
  • In this study, the amount of harmful fungi and bacteria contained in the drainage and culture medium from the paprika hydroponic facility is identified. In addition, by proposing the necessity of effective purification of discharged drainage, this study attempted to confirm the possibility of drainage reuse. Finally, this study provides basic data on the basis for calculating the need for purification facilities in the future, as well as improvements in horticulture facility for sustainable agriculture. As a result of the analysis, a total of 12 types of fungi were detected in paprika medium and 10 types of fungi were detected in the drainage, and their densities were 130 and 68, respectively. Among the fungi detected in the media and drainage of the paprika hydroponic facility, the fungi with the highest detection frequency are Fusarium, Phytophthora, and Pythium. In the case of bacteria, a total of 2 types of bacteria were detected in the paprika facilities, and the density was 28 and 23, respectively. Therefore, in order to reuse the drainage and settle the circulating hydroponic cultivation system, a water treatment process capable of appropriate treatment is required.

사경에 의한 잔디류의 내염성에 관한 연구 (Studies on the Salt - tolerance of Lawn Grasses in Sand Culture)

  • 홍종운
    • 아시안잔디학회지
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    • 제2권1호
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    • pp.5-30
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    • 1988
  • The object of this experiments was to know the salt tolerance of Fults and other lawn grasses. Fults, Olympic (Tall fescue, Festuca arundinacea Schred), Ceeping red fescue, Kentucky bluegrass and Zoysia grass (Z. japonica S.) were grown in hydroponics with vermiculite at various concentrations of NaCl. Hoagland's solution was used as the basic solution (control), and the concentrations of Cl to it were 1000, 2000, 3000, 4000 and l5OOOppm, respectively. Each was cultivated under the circumstances during 2 months. The results obtained are summarized as follows: 1.The growth of Fults, Olympic, Creeping red fescue and others were better at Cl lOOOppm than control. In the 5OOOppm application, Fescues become worse and 23.9% of them were withered. In concentration of Cl above 9OOOppm, it was impossible to live. 2.In the l0000~l1000ppm application, Olympic and Kentucky bluegrass were become worse and most of them died. 3.Fults were almost possible to live in the below of 9OOOppm, but they began to die in the above of 10000 ppm. 4.With the increasing concentration of Cl, plants were dwarfed and the number of stems, leaves and roots were reduced, but it was especially observed that the number of stolons of Creeping red fescue were increased at 1000~4000ppm. 5.Fults grass was the most salt tolerant turfgrass, but was impossible to live at salt level of about 36 millimhos (Exchange NaCI conductivity-ppm). Among the grasses, according to salt tolerance, they were arranged as follows. Fults > Zoysia japonica S. > Ky belugrass > Olympic grass > Creeping red fescue 6.The number of leaves, stems, tillers, and dry weight of Olympic grass, Fults and others were increased more at Cl 1OOOppm application than control, but in the above 4OOOppm application, those of plants were decreased. 7.The productivity of all grasses under the experiments was increased at 3.l75millimhos (Exchange NaCi conductivity ppm) in the concentration of Cl. The each dry-weight of Olympic, Creeping red fescue, Kentucky bluegrass and Zoysia grass was decreased at 8.85millimhos, and the weight of Fults grass was also decreased remarkably at 12.20millimhos. 8.As the result of this experiments, most plants grow normally at low concentration of NaCI-l000ppm. That seems to stimulate more the grasses to grow than non-salt.

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Stem Firmness and Flowering Response of Cut Lilies as Influenced by Medium Composition in Box Culture

  • Suh, Jeung-Keun
    • 한국생물환경조절학회:학술대회논문집
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    • 한국생물환경조절학회 2001년도 봄 학술발표논문집
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    • pp.45-50
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    • 2001
  • Stem firmness and flowering response of cut lily as influenced by medium composition (Control: Upland soil, Pt: Peatmoss, Pe: Perlite, Ve: Vermiculite, Rrh: Rotted rice-hull, RPt: Russian Peatmoss) were studied. For 'Casa Blanca', plant height and length of flower stalk increased when bulbs were planted in Pt:Rrh:Ve(1:1:1, v/v), and dried leaves of lower part plants decreased by RPt:Pe:Rrh(1:1:1, v/v). In case of 'Marco Polo' plant height and length of flower stalk increased with Pt:Rrh(1:1, v/v) as compared to other treatment, number of leaves and dried leaves increased when bulbs were planted in RPt:Pe:Rrh(1:1:1, v/v) as compared to control. Flowering of 'Casa Blanca' was promoted in Pt:Pe:Ve(1:1:1, v/v) and 'Marco Polo' was accelerated in Pt:Rrh:Ve(1:1:1, v/v). Flower length of 'Casa Blanca' was increased by RPt:Pe:Rrh(1 :1 :1, v/v) as compared with control and 'Marco Polo' was increased when bulbs planted to Pt:Rrh(1 :1, v/v). Flower-bud blasting of two cultivars was increased with Pt as compared with other treatment. Stem firmness of 'Casa Blanca' was increased by Pt:Pe:Ve (1:1 :1, v/v), and especially, stem firmness of upper part plants was increased by Pt:Rrh(1 :1, v/v) in 'Marco Polo' as compared to control plants. but generally, stem firmness of 'Casa Blanca' was not influenced with all cultural media as compared to control.

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화형이 우수한 진분홍색 절화장미 'Loving Heart' 육성 (Breeding of Cut Rose 'Loving Heart' with Good Flower Shape and Dark Pink Petal Color)

  • 한윤열;고진용;권민경
    • 화훼연구
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    • 제17권4호
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    • pp.332-335
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    • 2009
  • 경북농업기술원 구미화훼시험장에서 육성한 장미 'Loving Heart'는 수세가 강건하며 자홍색의 경교 R-6(♀)과 화형이 우수한 'Noblesse'(♂)를 2002년에 인공교배 한 후 계통선발 및 3차 특성검정을 거쳐 2007년에 최종선발하여 'Loving Heart'로 명명하였다. 'Loving Heart' 품종은 진분홍색 (RHS, Red-purple group 66-8)의 화형이 우수한 스탠다드 장미로 꽃직경은 10.3 cm, 꽃잎수는 55매, 절화수명은 12.3일정도이며 연간 절화수량이 127본/$m^2$으로 다수성이다. 특히, 절화품질이 우수하고 수세가 강건할 뿐 아니라 줄기가 곧게 자라고 줄기 윗부분에 가지가 없기 때문에 재배 및 취급이 용이하고 토양과 양액재배 모두 가능한 품종이다.

수경재배 잎들깨의 생육과 수량에 미치는 코이어 배지의 혼합비율 효과 (Effect of Coir Substrate Mixing Ratios on the Growth and Yield of Perilla Leaves under Hydroponics)

  • 박평식;박종원;현혜경;김현숙;함수상;김학헌;최효길
    • 한국환경과학회지
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    • 제33권1호
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    • pp.17-25
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    • 2024
  • This study aimed to determine the most suitable coir substrate mixing ratio for optimizing the growth and yield of the "lpduelkkae 1" cultivar. We comprehensively analyzed the physicochemical properties, growth, and yields of four different substrate combinations: perlite (coir with mixing ratios of 70:30 (PC30), 50:50 (PC50), and 30:70 (PC70)) and 100% coir (C100). The results revealed substantial differences in substrate properties. C100 exhibited the highest total porosity and the lowest solid phase, indicating excellent air permeability. The pH levels and electrical conductivity (EC) values ranged from 5.4-6.8 and 1.2-3.1 dS·m-1, respectively. Leaf growth parameters, including length, width, and dry weight, showed positive correlations with high coir ratios, except for PC30. PC70 and C100 outperformed other substrates in stem growth, exhibiting superior stem diameter and fresh and dry weights. The quantity of marketable leaves was the highest in the C100 substrate. Furthermore, C100 comprised integrated levels of essential nutrients, such as Ca and Mg, owing to its high coir content. In conclusion, a coir ratio of approximately 70% (v/v) should be maintained in the substrate for creating an optimal cultivation environment. Furthermore, the selection of humidity-resistant varieties as well as precise nutrient and moisture management for different seasons and growth stages are crucial for a successful perilla leaf hydroponic cultivation.