• Korean Journal of Medicinal Crop Science
• /
• v.16 no.6
• /
• pp.439-445
• /
• 2008

This study was performed to investigate a methodology of improving immune activities of Berberis koreana Palibin by ultra high pressure on low temperature extraction process. Extraction yield was enhanced up to more than 35% by above process, compare to the control and extraction average. The cytotoxicity on human kidney cell (HEK293) was showed below 20.4%, 21.6% in adding 1.0 mg/ml of the highest concentration. Generally, the extracts by ultra high pressure extraction process showed less toxicity about 5% than the other processes. It could tell that toxic materials that existing in the plant could be reduced or broken by ultra high pressure process due to can be broken bond such the hydrogen bond, the electrostatic bond, the van der Waals bond, and the hydrophobic bond, can be broken by high pressure. The result could be employed to develop a new type of functional food from B. koreana Palibin by low temperature high pressure process.

• Applied Chemistry for Engineering
• /
• v.7 no.4
• /
• pp.707-714
• /
• 1996

Extraction behaviour of Np with DEHPA(di-(2-ethyhexyl)Phosphoric acid) from the low nitric acid solution(below 1M $HNO_3$) containing $H_2O_2$ as a reducing agent was studied at a batch system in order to establish the conditions of extraction and stripping and to enhance the extraction rate. As results, it was confirmed that the Np was mainly the pentavalent oxidation state in the low nitric acid solution. The extraction yield of Np was increased with increasing the concentration of DEHPA and $H_2O_2$ and decreased more rapidly with the increase of $HNO_3$ concentration. It was also found that the Np could be extracted into DEHPA even without the addiction any redox agents, although the extraction yield is rather low as about 70%. The extraction rate was proportional to the 0.516 power of $H_2O_2$ concentration and inversely proportional to 0.483 power of $HNO_3$ concentration as follows. $d[Np(V)]/dt=-1.391{\times}10^{-2}[H_2O_2]^{0.516}[HNO_3]^{-0.483}[Np(V)]$ Regardless of the $H_2O_2$, the Np extracted in the organic phase was effectively stripped to the aqueous phase with $H_2C_2O_4$. The Np could be stripped more than 92 % with 0.5M $H_2C_2O_4$.

• Journal of the Korean Applied Science and Technology
• /
• v.5 no.1
• /
• pp.25-30
• /
• 1988

In order to develope the method of quantitative determination of lipid hydroperoxide in human blood serum, we tried the ferrothiocyanate method to total lipids extracted by Bligh-Dyer method and obtained the results as follows. 1. The maximum absorbance showed at the concentration of Mohr's solution, 0.127M at pH 1.70 and ammonium thiocyanate solution, 3.95M in the ferrothiocyanate method. 2. When hydrogen peroxide, cumene hydroperoxide, and oxidized linoleic acid were added to serum, and extracted them by Bligh-Dyer method to examine the extraction efficiency, we confirmed that cumene hydroperoxide and oxidized linoleic acid were extracted in $CHCI_3$ phase, and hydrogen peroxide in $MeOH-H_2O$ phase, respectively. 3. The concentration of lipid hydroperoxide of total lipids extracted from normal adult serum was $2.0{\times}10^{-5}M$, and increased proportionally the concentration of lipid hydroperoxide by increasing the amount of serum. 4. When we compared the total lipids extracted by Bligh-Dyer method and total lipids extracted after lipoprotein is precipitated by Yagi method in human blood serum, the concentration of lipid hydroperoxide was showed nearly the same value. From our results, we concluded that the concentration of lipid hydroperoxide in human blood serum could be determined quantitatively by ferrothiocyanate method.

• Archives of Pharmacal Research
• /
• v.15 no.1
• /
• pp.52-57
• /
• 1992

The molecular structure of a natural compound was determined by single crystal X-ray diffraction analysis. The compound was isolated by methanol extraction and repeated chromatography from the stem of Paulownia tomentosa. Yellow prismatic crystals of the compound, which were recrystallized from tetrahydrofuran, are triclinic, with a = 7.310 (6), b = 10.753(6), c = 11.586(5) ${\AA}.\;\alpha= 93.30(6),\;\beta=105.62(10),\;\gamma=109.49(7)^\circ,\;D_x=1.514,\;D_m=1.51 g/cm^3$, space group P1 and Z = 2. The structure was solved by direct method, and refined by least-squares procedure to the final R-value of 0.032 for 1271 independent reflections $(F\le3\sigma{(F))}$. The compound is one of new furanquinone analogue. The molecule has a nearly planar conformation with an intramolecular hydrogen bond. In the crystal, the planar molecules are arranged as a prallel sheet-like pattern, and these stackings are stabilized by the O-H...O type intermolecular hydrogen bonds. The other intermolecular contacts appear to be the normal van der Waals interactions.

• Applied Chemistry for Engineering
• /
• v.22 no.1
• /
• pp.48-55
• /
• 2011

It was found that the absorbent mixed with 2-aminoethanol and others has been applied to remove them via chemical neutralization. The absorbent of natural second metabolites was achieved by a removal efficiency of 20~30% by itself depending on treatment conditions, but the complex absorbent mixed with 0.2% amine chemical provides the removal efficiency of over 98%. Optimal removal efficiencies have been examined against two major parameters of the temperature and pH to remove ammonia and hydrogen sulfide gases. The chemical analysis was also performed to analyze the composition of an essential oil by GC-MS. The monoterpenes in an essential oil reacted with odorous compounds by neutralization and their reaction mechanism was partially elucidated.

• Journal of Korea Technical Association of The Pulp and Paper Industry
• /
• v.46 no.1
• /
• pp.1-10
• /
• 2014

Xylanase (X) derived from Aurreobasidium pullulans and laccase-mediator system (LM) using Trichophyton sp. LKY-7 laccase (TrL) and N-hydroxy-2-pyridone analogue (NHP) as a mediator were applied in hardwood kraft pulp (HwKP) bleaching. The individual and the synergistic effects of X and LM stage were investigated in the enzymatic bleaching of HwKP. Also, the effects of subsequent alkaline extraction (E) and alkaline/hydrogen peroxide treatment (P) were examined. In X or LM treatment alone, an appreciable bleaching effect of HwKP was not observed, whereas subsequent E or P stage enhanced the increase of brightness and the decrease of kappa number. Especially, P stage significantly enhanced the bleaching effect of pulp. Bleaching of HwKP with XLM sequentially gave significantly higher pulp brightness and lower kappa number than that obtained after the treatment of HwKP with X+LM simultaneously. When HwKP was sequentially treated with XLM followed by P stage, the brightness increased by about 11% ISO and the kappa number decreased by about 3.6 in comparison with the initial pulp. Xylanase and laccase were strongly inactivated by NHP both in the absence and the presence of pulp.

• Journal of the Korean Chemical Society
• /
• v.16 no.6
• /
• pp.361-368
• /
• 1972

The organic solvent mixture of 5 % trioctylamine and 5 % n-octyl alcohol in n-hexane could be loaded with tungsten to 62 g $WO_3$/1 whereas the amine alone could be loaded only to 37 g $WO_3$/1. Molecular interaction between amine and tungstate ion during the solvent extraction process and the synergistic effect of alcohol in enhancing the solubility of the amine-tungstate complex have been studied by NMR spectroscopy. Tungsten has been shown to be extracted as a polymeric tungstate ion into the organic phase to form a trioctyl ammonium polytungstate salt, which was more stabilized in the hydrocarbon medium by interacting with alcohol via hydrogen bonding between the tungstate ion and alcohol.

• Geotechnical Engineering
• /
• v.11 no.4
• /
• pp.87-98
• /
• 1995

Laboratory tests were performed on modeling of in situ remediation of contaminated soils by aqueous solution extraction, thus investigating the feasibility of in situ treatments of soil to promote desorption of organic hazardous wastes. The investigation was conducted using phenol, aniline, quinoline, and 2-napthol adsorbed onto a UH40 soil, and various aqueous solutions were used to desorb, or otherwise remove, these organic contaminants. Decontaminants consisted of deionized water as a reference, hydrogen peroxide, acidy, bases, and surfactants. In situ conditions were modeled in the laboratory by permeating potential extracting liquids through reconstituted, contaminated soil specimens under controlled hydraulic gradients and stress condition through flexible wall permeameter tests. Sodium hydroxide desorbed phenol effectively. Aniline was effectively descorbed by nonionic surfactant. Anionic surfactant remediated quinoline and 2-napthol.

• Journal of The Korean Society of Clinical Toxicology
• /
• v.1 no.1
• /
• pp.12-20
• /
• 2003

Cyanides and hydrogen sulfide ($H_2S$) are major chemical asphyxiants. They have common mechanism of action which inhibit cellular respiration and induce histotoxic hypoxia. They do not generate ATP, and all processes dependent on ATP are stopped. No extraction of $O_2$ from blood decreases AV $O_2$ differences, and the shift to anaerobic glycolysis brings about lactic acidosis with high anion gap. The mainstay of the treatment is rapid treatment with appropriate use of antidotes. However, there are several differences between cyanides and $H_2S$. First, $H_2S$ is not metabolized by enzymes such as thiosulfate. Thus thiosulfate does not play any role in treatment of $H_2S$. Second, $H_2S$ is a more potent inhibitor of cytochrome aa3 than cyanide. Third, $H_2S$ induces more divergent neurologic sequele than cyanide. Finally, $H_2S$ is not absorbed via skin.

• Korean Journal of Food Science and Technology
• /
• v.20 no.3
• /
• pp.405-411
• /
• 1988

In this study, the treatment of $H_2O_2$, papain and acylation on the protein isolate obtained from defatted sesame oil cake for the changes in amino acids composition, color, digestibility and some functional characteristics of this protein were investigated. The results showed that $H_2O_2$, papain and papain $H_2O_2$ treatment increased the rates of the protein extraction and content of protein in protein isolates when those values were compared to the alkali treatment. Also amino acid composition of the protein isolates were comparatively good, and color, and functional properties and pepsin digestibility were markedly improved. The amount of lysine, the first limiting amino acid of this protein was increased. The solubility, bulk density, water absorption, fat absorption and emulsifying properties were remarkably improved while foaming properties and digestibility was slighly decreased.