• Title/Summary/Keyword: hybrid number

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Interspecific Hybridization between Pleurotus cornucopiae and Pleurotus florida Following Protoplast Fusion (원형질체(原形質體) 융합(融合)에 의한 노랑느타리버섯과 사철느타리버섯의 종간(種間) 교잡(交雜))

  • Yoo, Young-Bok
    • The Korean Journal of Mycology
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    • v.20 no.2
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    • pp.118-125
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    • 1992
  • Interspecific fusion products were obtained by protoplast fusion induced by polyethylene glycol, from auxotrophic mutants, Pleurotus cornucopiae and P. florida. The fusants were classified into allodiploidy, stable heterokaryon, and spontaneously segregated heterokaryons. Fruiting body of the clamped or clampless fusants was produced by light-dark cycle on the sawdust medium in glass bottles. Most of these clampless fusants produced mature basidiocarps. The pilei showed various mixed colors resembling the parents. All fruit bodies presented clamp connections except two fusants. When small tissues of stipe from basidiocarps were cultured on a complete medium, mycelial colonies grew more vigorously than that of the original clampless fusants. Five fusants in three crosses were analysed with the distribution of progenies and segregation of genetic characters by random spore analyses. The genetic markers were shown to segregate and recombine in the first generation of monospores isolated from basidiocarps. The analysis indicated the heter-okar-yosis and strong evidence for haploidy of vegetative nuclei, a sexual cycle consisting of nuclear fusion and meiosis. Genotypes of a large number of auxotiophic progenies were not detected. The aberration ratio of segregants indicated the gene interaction resulting from different genome structure between vegetatively incompatible species.

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Fine Grained Resource Scaling Approach for Virtualized Environment (가상화 환경에서 세밀한 자원 활용률 적용을 위한 스케일 기법)

  • Lee, Donhyuck;Oh, Sangyoon
    • Journal of the Korea Society of Computer and Information
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    • v.18 no.7
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    • pp.11-21
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    • 2013
  • Recently operating a large scale computing resource like a data center becomes easier because of the virtualization technology that virtualize servers and enable flexible resource provision. The most of public cloud services provides automatic scaling in the form of scale-in or scale-out and these scaling approaches works well to satisfy the service level agreement (SLA) of users. However, a novel scaling approach is required to operate private clouds that has smaller amount of computing resources than vast resources of public clouds. In this paper, we propose a hybrid server scaling architecture and related algorithms using both scale-in and scale-out to achieve higher resource utilization rate for private clouds. We uses dynamic resource allocation and live migration to run our proposed algorithm. Our propose system aims to provide a fine-grain resource scaling by steps. Thus private cloud systems are able to keep stable service and to reduce server management cost by optimizing server utilization. The experiment results show that our proposed approach performs better in resource utilization than the scale-out approach based on the number of users.

In-situ Analysis of Temperatures Effect on Electromigration-induced Diffusion Element in Eutectic SnPb Solder Line (공정조성 SnPb 솔더 라인의 온도에 따른 Electromigration 확산원소의 In-situ 분석)

  • Kim Oh-Han;Yoon Min-Seung;Joo Young-Chang;Park Young-Bae
    • Journal of the Microelectronics and Packaging Society
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    • v.13 no.1 s.38
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    • pp.7-15
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    • 2006
  • In-situ observation of electromigration in thin film pattern of 63Sn-37Pb solder was performed using a scanning electron microscope system. The 63Sn-37Pb solder had the incubation stage of electromigration for edge movement when the current density of $6.0{\times}10^{4}A/cm^2$ was applied the temperature between $90^{\circ}C\;and\;110^{\circ}C$. The major diffusion elements due to electromigration were Pb and Sn at temperatures of $90-110^{\circ}C\;and\;25-50^{\circ}C$, respectively, while no major diffusion of any element due to electromigration was detected when the test temperature was $70^{\circ}C$. The reason was that both the elements of Sn and Pb were migrated simultaneously under such a stress condition. The existence of the incubation stage was observed due to Pb migration before Sn migration at $90-110^{\circ}C$. Electromigration behavior of 63Sn-37Pb solder had an incubation time in common for edge drift and void nucleation, which seemed to be related the lifetime of flip chip solder bump. Diffusivity with $Z^*$(effective charges number) of Pb and Sn were strongly affect the electromigration-induced major diffusion element in SnPb solder by temperature, respectively.

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Plant Regeneration by Anther Culture of Tetraploid Populus alba L.X P.glandulosa Uyeki (4배체 현사시나무 (Populus alba L. X P. gludulosa Uyeki)의 약배양에 의한 식물체 재분화)

  • Son, Sung-Ho;Kim, Jung-Hee;Moon, Heung-Kyu;No, Eun-Woon;Lee, Yoon-Hee;Kim, Mi-Hee;Park, Jin-Sun;Lee, Yong-Wook;Yoon, Yang;Lee, Seok-Gu
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.3
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    • pp.121-126
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    • 1995
  • Diploid plants were obtained by anther culture of tetraploid poplar(Populus alba L. X P.glandutosa Uyeki). The effect 2,4D on callus formation from anther culture was greater than any other auxins tested. The highest average number of multiple shoots per callus was obtained when zeatin was used at levels of 6-8 ${\mu}$M. Regenerated shoots were excised and transferred to MS basal medium. Rooted plantlets were subsequently transferred to pots containing artificial soil mix. Finally 100 plane were transplanted in nursery located in forest Genetics Research Institute. for the 300 anther clones growing in greenhouse for 6 months after transplanting, 33% were slow-growing, 47% were rapid-growing and 20% had huge leaf size with rapid-growing characteristics. Chromosome study showed a narrow range of variation from diploid to tetraploid. DNA polymorphism studies using various RAPD markers revealed some extend of differences among the anther-clones in their band pattern.

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Plant Regeneration via in Vitro Culture of Ovule Obtain by Intergeneric Crossing Between Citrus junos Sieb. et Tanaka and Poncirus trifoliata Raf. (유자와 탱자의 속간교잡후 배주배양에 의한 식물체 유기)

  • 이만상;남궁승박
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.6
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    • pp.317-322
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    • 1995
  • As a basic research for breeding new varieties, reciprocal -intergeneric crosses between Citrus junos and P.trifoliata were made. F$_1$ hybrid production using in vitro ovule culture, gametogenesis, and fertilization phenomena were investigated. Frequency of fruit set resulting from crossing of Citrus junos and Poncirus Trifoliata was 16.6% while that of Poncirus Trifoliata and Citrus junos was 11.7%. Callus formation occurred well when ovules at the 6th week after pollination were cultured on MT (Murashige and Tucker) medium supplemented with zeatin 0.5 mg/L and NAA 1.0 or 3.0 mg/L. Immature ovules developed into mature embryos of the MT medium supplemented with 2,4-D 0.1 or 3.0 mg/L. Immature ovules developed into mature embryos of the MT medium supplemented with 2,4 D 0.1 or 0.5 mg/L. The invitro germination rates of 20-week-old ovules set C. junos $\times$ P. Trifoliata and P. Trifoliata $\times$ C. junos were 54.5% and 48.6%, respectively. The emergence ratios of trifoliate hybrids obtained by C. junos $\times$ P. Trifoliata and P. Trifoliata $\times$ C. junos were 56.7% and 100%, respectively. The chromosome number of C. junos and P. Trifoliata was n = 9 or 2n = 18, and the sizes of their pollen grain were 33.75 $\mu$ and 25.0 $\mu$. The length and width of embryo sac in C. junos and P. Trifoliata were 69.38~79.23 $\mu$ and 27.50~38.56 $\mu$, and those of egg cells were 17.50~41.50 $\mu$ and 6.25~8.12$\mu$. Fertilization of C. junos and P. trifoliata terminated 72 h after pollination.

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Studies on the Behavioral response in Silkworm larvae, Bombyx mori. Genetic Analysis and Correlated Response to Economic Characters of Walking Distance (가잠유충의 행동반응성에 관한 연구)

  • Park, Yeon-Gyu;Lee, Sang-Pung;Lee, Ho-Ju
    • Journal of Sericultural and Entomological Science
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    • v.30 no.1
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    • pp.8-19
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    • 1988
  • The objective of the present study was to investigate the behavioral responses of sillkworm (Bombyx mori) larvae to: the dominant effect of walking distace: and the relationships between walking distance and the various economic characters on yellow sheets. The means of walking distances of progeny from F2 and F1 F1 and F2 generations, which was a hybrid between the long and the short walking distances, were shorter than those of parents. This may indicate that the genes for the short walking distances were partially dominant over those for the longwalking. Estimated broad sense heritability of larval walking distance was different for each larval instar, which fell into the range of 52∼84%. Genetic correlation was apparent between walking distances of all larval stages and some economic characters. The number of cocoons per liter and the period from incubation to emergence have shown the positive genetic correlation with walking distances. The cocoon yield, the sing cocoon weight, and the cocoon shell weight have shown to be in the negative correlation with walking distances. No correlation was observed between walking distances of the mature larvae and any economic characters. The analysis by the path coefficient showed negative direct effect of walking distances on the cocoon yield, and positive indirect effect on the single cocoon weight and the cocoon shell weight.

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An Implementation of IEEE 1516.1-2000 Standard with the Hybrid Data Communication Method (하이브리드 데이터 통신 방식을 적용한 IEEE 1516.1-2000 표준의 구현)

  • Shim, Jun-Yong;Wi, Soung-Hyouk
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.37C no.11
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    • pp.1094-1103
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    • 2012
  • Recently, software industry regarding national defense increases system development of distributed simulation system of M&S based to overcome limit of resource and expense. It is one of key technologies for offering of mutual validation among objects and reuse of objects which are discussed for developing these systems. RTI, implementation of HLA interface specification as software providing these technologies uses Federation Object Model for exchanging information with joined federates in the federation and each federate has a characteristic that is supposed to have identical FOM in the federation. This technology is a software which is to provide the core technology which was suggested by the United state's military M&S standard framework. Simulator, virtual simulation, and inter-connection between military weapons system S/W which executes on network which is M&S's core base technology, and it is a technology which also can be used for various inter-connection between S/W such as game and on-line phone. These days although RTI is used in military war game or tactical training unit field, there is none in Korea. Also, it is used in mobile-game, distribution game, net management, robot field, and other civilian field, but the number of examples are so small and informalized. Through this developing project, we developed the core technique and RTI software and provided performance of COTS level to improve communication algorithms.

An Admission Control Mechanism to guarantee QoS of Streaming Service in WLAN (WLAN에서 스트리밍 서비스의 QoS를 보장하기 위한 승인 제어 기술)

  • Kang, Seok-Won;Lee, Hyun-Jin;Lee, Kyu-Hwan;Kim, Jae-Hyun;Roh, Byeong-Hee
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.34 no.6B
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    • pp.595-604
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    • 2009
  • The HCCA reserves the channel resources based on the mean data rate in IEEE 802.11e. It may cause either the waste of channel resource or the increase of transmission delay at MAC layer if the frame size is rapidly varied when a compressed mode video codec such as MPEG video is used. To solve these problems, it is developed that the packet scheduler allocates the wireless resource adaptation by according to the packet size. However, it is difficult to perform the admission control because of the difficulty with calculating the available resources. In this paper, we propose a CAC mechanism to solve the problem that may not satisfy the QoS by increasing traffic load in case of using EDCA. Especially, the proposed CAC mechanism calculates the EB of TSs using the traffic information transmitted by the application layer and the number of average transmission according to the wireless channel environment, and then determines the admission of the TS based on the EB. According to the simulation results of the proposed CAC mechanism, it admitted the TSs under the loads which are satisfied within the delay bound. Therefore, the proposed mechanism guarantees QoS of streaming services effectively.

Effect of Day-length Extension Treatment Using LED on Growth and Flowering of Freesia hybrid 'Yvonne' (LED를 이용한 장일 처리가 프리지아(Freesia hybrida) '이본느'의 생육 및 개화에 미치는 영향)

  • Lee, Jin-Jae;Hwang, Ji-Hye
    • Horticultural Science & Technology
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    • v.32 no.6
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    • pp.794-802
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    • 2014
  • The aim of this study was to determine the effect of day-length extension treatment using LED lighting (blue, green, red, or 3 mixed) on vegetative growth and flowering of freesia 'Yvonne', in comparison to that using glow lamps and metal halide lamps. Lighting treatments were imposed from 5 PM to 8 PM for 150 days from after flower bud differentiation to flowering end. For the period from leaf emergence to floral bud formation, no light source affected plant height but the number of leaves was decreased by the metal halide lamp. The highest SPAD value in the flower bud developing period occurred in the metal halide lamp treatment. The time of flowering was advanced by blue or green LED treatment compared to the no lighting control. The green LED lighting advanced flowering by 6 and 8 days compared to no lighting and metal halide lamp treatment, respectively. The blooming period tended to be shortened by the red LED treatment. As for the flower characteristics of 'Yvonne', floret length and width, and the weight of cut flowers were highest in the metal halide lamp treatment. Red LED decreased corm width and weight of 'Yvonne' while glow lamp decreased height and weight. Starch contents in corm were not influenced by the lighting source. Our results indicate that the green LED lighting advanced the time of flowering and the metal halide lamp was good for cut-flower quality.

Factors Affecting Efficiency of Anther Culture and Phenotypic Characteristics of Anther -derived Progeny in Broccoli (녹색꽃양배추 (Brassica oleracea L. var. italica)의 약배양 효율증진과 약유래 계통의 특성)

  • 남시춘;윤광현;백기엽
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.3
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    • pp.189-195
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    • 1999
  • This study was carried out to clarify several factors affecting embryogenesis from anther culture of nine cultivars in Brassica oleracea L. var. italica and to investigate the characteristics of plants derived from anther culture. Androgenesis from anther culture was elevated on the B5 medium supplemented with 0.1mg/L NAA, 0.1mg/L 2.4-D and 10% sucrose. Embryo production in liquid medium was five-fold higher than solid medium. High temperature treatment at 35$^{\circ}C$ for one day before transfer to culture room maintained at $25^{\circ}C$ had effective to induce embryogenesis of cultured anthers but extended treatment at 35$^{\circ}C$ decreased significantly the percent of embryogenesis. Frequency of embryogenesis from cultured anthers exhibited significant difference from 2.8% in 'Green Valiant' to 21% in 'Haisi' as affected by genotypes. Percent of spontaneously dihaploid among regenerated plants from anther culture was ranged from 62 to 74% as affected by the genotypes. Characteristic in relation to plant height, number of leaves and branches, and size of head from anther-derived plants showed differential variation in 'Rokguray' and 'Haisi'. Among these charaters obtained from two cultivars, five lines were selected for early maturity, long plant height and large head. Selected lines were used as breeding meterials for F$_1$ hybrid.

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