In this study, a Korean native cattle strain (Hanwoo) evidencing high performance in terms of both meat quality and quantity was employed in the generation of 150,000 BAC clones with an average insert size of 140 kb, and corresponding to about a 6X coverage of bovine chromosomal DNA. The BAC clones were pooled in a mini-scale via three rounds of a pooling protocol, and the efficiency of this pooling protocol was evaluated by testing the accuracy of accessibility to the positive clones, via a PCR-based screening method. Two sets of primers designed from each of two known genes were tested, and each yielded 2 or 3 positive clones for each gene, thereby indicating that the BAC library pooling system was appropriate with regard to the accession of the target BAC clones. Analyses of $3.3{\times}10^6$ base pairs obtained from the 7,090 BAC end sequence (BES) showed that 34.88% of the DNA sequence harbored the repetition sequence. Analysis of the 7,090 BES to the $1^{st}$ and $2^{nd}$ generation radiation hybrid map of the cattle genome, using the COMPASS program designed for the construction of a cattle-human comparative mapping, resulted in the localization of a total of 1,374 clones proximal to 339 $1^{st}$ generation markers, and 1,721 clones proximal to 664 $2^{nd}$ generation markers. Collectively, the BAC library and pooling system of the BAC clones from the Korean cattle, coupled with the chromosome-localized BAC clones, will provide us with novel tools for the excavation of desired clones for genome mapping and sequencing, and will also furnish us with additional information regarding breed differences in cattle.
A stereoscopic 3D video service is able to provide a 3D video service while keeping backward compatibility with the existing 2D video service. In the terrestrial digital television (DTV) system, a stereoscopic video codec is required to have high coding efficiency in order to provide a 3D video service in the same channel capacity. A hybrid codec consisting of MPEG-2 for base video and H.264/AVC or HEVC for 3D auxiliary video is considered. Furthermore, Non-Real-Time (NRT) delivery of stereoscopic video is also considered as a service scenario for 3DTV services to overcome the limited bandwidth. In this paper, we propose a stereoscopic video coding scheme using adaptive loop filter (ALF) which had been considered in HEVC as a pre-/post-filter for enhancing coding efficiency in NRT-based 3DTV services. In order to apply ALF as a post-filter to the reconstructed additional view coded by H.264/AVC, we devise a method in which ALF is adaptively applied based on a structure determined by using macroblock (MB) coding information such as MB mode type and reference index instead of coding unit (CU) structure on which ALF is applied in the HEVC. Experimental results shows that the proposed stereoscopic video coding scheme applying ALF obtains up to 24.9% gain of bit saving.
Artificial Organism-used application areas are expanding at a break-neck speed with a view to getting things done in a dynamic and Informal environment. A use of general programming or traditional hi methods as the representation of Artificial Organism behavior knowledge in these areas can cause problems related to frequent modifications and bad response in an unpredictable situation. Strategies aimed at solving these problems in a machine-learning fashion includes Genetic Programming and Evolving Neural Networks. But the learning method of Artificial-Organism is not good yet, and can't represent life in the environment. With this in mind, this research is designed to come up with a new behavior evolution model. The model represents behavior knowledge with Classification Rules and Enhanced Backpropation Neural Networks and discriminate the denomination. To evaluate the model, the researcher applied it to problems with the competition of Artificial-Organism in the Simulator and compared with other system. The survey shows that the model prevails in terms of the speed and Qualify of learning. The model is characterized by the simultaneous learning of classification rules and neural networks represented on chromosomes with the help of Genetic Algorithm and the consolidation of learning ability caused by the hybrid processing of the classification rules and Enhanced Backpropagation Neural Network.
The Journal of Korean Institute of Communications and Information Sciences
/
v.37C
no.11
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pp.1094-1103
/
2012
Recently, software industry regarding national defense increases system development of distributed simulation system of M&S based to overcome limit of resource and expense. It is one of key technologies for offering of mutual validation among objects and reuse of objects which are discussed for developing these systems. RTI, implementation of HLA interface specification as software providing these technologies uses Federation Object Model for exchanging information with joined federates in the federation and each federate has a characteristic that is supposed to have identical FOM in the federation. This technology is a software which is to provide the core technology which was suggested by the United state's military M&S standard framework. Simulator, virtual simulation, and inter-connection between military weapons system S/W which executes on network which is M&S's core base technology, and it is a technology which also can be used for various inter-connection between S/W such as game and on-line phone. These days although RTI is used in military war game or tactical training unit field, there is none in Korea. Also, it is used in mobile-game, distribution game, net management, robot field, and other civilian field, but the number of examples are so small and informalized. Through this developing project, we developed the core technique and RTI software and provided performance of COTS level to improve communication algorithms.
Journal of the Korean Society for information Management
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v.25
no.4
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pp.283-308
/
2008
In this study, we testify that network structural attributes of a citation network can explain other aspects of journal citation behaviors and the importances of journals. And we also testify various citation impact indicators of journals including JIF and h-index to verify the difference among them especially focused on their ability to explain an institution's local features of citation behaviors. An institutional citation network is derived using the articles published in 2006-2007 by biotechnology faculties of Y University. And various journal citation impact indicators including JIF, SJR, h-index, EigenFactor, JII are gathered from different service sites such as Web of Science, SCImago, EigenFactor.com, Journal-Ranking.com. As a results, we can explain the institution's 5 research domains with inter-citation network. And we find that the co-citation network structural features can show explanations on the patterns of institutional journal citation behavior different from the simple cited frequency of the institution or patterns based on general citation indicators. Also We find that journal ranks with various citation indicators have differences and it implies that total-based indices, average-based indices, and hybrid index(h-index) explain different aspects of journal citation pattern. We also reveal that the coverage of citation DB doesn't be a matter in the journal ranking. Analyzing the citation networks derived from an institution's research outputs can be a useful and effective method in developing several library services.
In the 21C, each nation controls exhaust fumes from automobiles and makes an effort to develop alternative energy because of serious environmental problem. Jechon area has many historical and cultural archeological sites. And Jechon city sponsors various cultural events. But the way of transportation which is connected with Jecheon and around sightseeing places is general and not ready yet. Therefore, if a special means of vehicle is developed, it could play an another role of sightseeing resources. Special identity of Jecheon area for establishment of green vehicle traffic system which gives Jecheon area specific character was investigated for theoretical background. Traffic system was studied for establishment of direction through existent successful case study. Moreover content, method, structure and advantage & shortcoming etc. of vehicle that use green energy resource such as solar car, fuel cell car, hybrid car, natural gas car etc. were examined. The suitable means of vehicle for Jechon area was proposed to three directions with research and investigation. After comparison and investigation by inquiry of each section's experts, the most suitable traffic system of which energy resource of car, form of vehicles, the complement, dimension of vehicles etc. were decided. Design proposal should be drawn according to process of automobile design in decided direction. Special Exterior design of vehicle that use green energy resource connecting Jecheon and around area should be suggested in Jecheon City Hall and Chungchong-bukdo provincial office for vivify image of cleanliness area.
Kim, Hyun;Yu, Dae Jung;Choe, Changyong;Seong, Hwan-Hoo
Reproductive and Developmental Biology
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v.39
no.3
/
pp.77-81
/
2015
This study was carried out to evaluate the effects of embryonic stage and toxicities of cryoprotectant on the rates of survival and development of the cryopreserved mouse early embryo and finally to establish the cryopreservation method of surplus embryos obtained during assisted reproductive technology. Toxicities of two cryoprotectants, dimethyl sulfoxide (DMSO) and ethylene glycol (EG) were investigated using a murine embryo model. Female F-1 mice were stimulated with gonadotropin, induced ovulation with hCG and mated. Two cell embryos were collected and cultured after exposure to either DMSO or EG. Embryo development was evaluated up to the blastocyst stage. The total cell count of blastocysts that were treated with DMSO ($68.1{\pm}24.1$) at the 2-cell stage was significantly lower than that were treated with EG ($81.2{\pm}27.0$) or the control ($99.0{\pm}18.3$) (p<0.001). On comparison of two cryoprotectant treated groups, the DMSO treated group showed a decreased cell count compared with the EG treated group (p<0.05). Both DMSO ($15.4{\pm}1.5$) and EG ($10.2{\pm}1.4$) treated groups showed higher apoptosis rates of cells in the blastocyst compared with the control ($6.1{\pm}0.9$, p<0.0001). In addition, the DMSO treated group showed more apoptotic cells than the EG treated group (p<0.001). The potential toxicity of cryoprotectants was uncovered by prolonged exposure of murine embryos to either DMSO or EG at room temperature. When comparing two cryoprotective agents, EG appeared to be less toxic than DMSO at least in a murine embryo model.
This study aims at analyzing the characteristics of deconestructionism expressed in Issey Myake's fashion who is one of the most creative designers in the world. Method and contents of this study are as follows. Through the review of literatures, three major categories such as interminacy of meaning, decentring, and intertextuality and eight subcategories have been identified as major features of deconestructionism. These subcategories for content analysis were selected to examine the characteristics of deconstructionism expressed in Issey Myake's design in depth. Interminacy of meaning was divided into unstructured and unconstructed factors while decentring into qualities of cross-gender, subculture, anti-fashion, and post-humanism. Lastly, intertextuality was classified into factors of disordered mixing mode and mixed styles. Materials for analysis were chosen from total 349 designs on the website,www.firstview.com, which carries Issey Miyake's collection from 2002 S/S through 2006 F/W. 2 fashion major analysts including the researcher participated in 1st analysis and two another fashion major analysts participated in 2nd analysis. The final level of consistency between analysts was over 97% for all categories. The data analysis quantitatively evaluates the frequency of each category and qualitatively evaluates the characteristics of design. The result is as follows. First, the avant-garde facets of inter-textuality are most prominent. In particular, Miyake has introduced the various novelly textiles to fashion and employed the mixtured styles, not to copy them but to create hybrid designs by using pastiches and mixing fashion styles. Moreover, he has used various fabrics in order to inflict shock and contrast but still create subtle harmony, thus enriching his fashion. Second, the indeterminacy of meaning is the second most frequently shown facet of Miyake's fashion design. Miyake has employed unconstructed expression techniques, which has projected unusual images free from basic forms of designs and also has used the unstructured styles which dismantle harmony and balance-the common rule of design. He has made a new attempt to acknowledge clothing as an expressive artwork by allowing clothes to take a complete form when a person actually wears them. He has demonstrated a unique perspective on cloth construction and presented a love for artistic collaboration in the development of his collections A distinct manufactured aesthetic is exaggerated, even hyperbolic. Thirdly, the aspects of decentralization were also shown in Miyake's design, however, it has been visibly decreased after 2002 S/S.
Korean Journal of Agricultural and Forest Meteorology
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v.13
no.1
/
pp.35-40
/
2011
While high-definition precipitation maps with a 270 m spatial resolution are available for South Korea, there is little information on geospatial availability of precipitation water for the famine - plagued North Korea. The restricted data access and sparse observations prohibit application of the widely used PRISM (Parameter-elevation Regressions on Independent Slopes Model) to North Korea for fine-resolution mapping of precipitation. A hybrid method which complements the PRISM grid with a sub-grid scale elevation function is suggested to estimate precipitation for remote areas with little data such as North Korea. The fine scale elevation - precipitation regressions for four sloping aspects were derived from 546 observation points in South Korea. A 'virtual' elevation surface at a 270 m grid spacing was generated by inverse distance weighed averaging of the station elevations of 78 KMA (Korea Meteorological Administration) synoptic stations. A 'real' elevation surface made up from both 78 synoptic and 468 automated weather stations (AWS) was also generated and subtracted from the virtual surface to get elevation difference at each point. The same procedure was done for monthly precipitation to get the precipitation difference at each point. A regression analysis was applied to derive the aspect - specific coefficient of precipitation change with a unit increase in elevation. The elevation difference between 'virtual' and 'real' surface was calculated for each 270m grid points across North Korea and the regression coefficients were applied to obtain the precipitation corrections for the PRISM grid. The correction terms are now added to the PRISM generated low resolution (~2.4 km) precipitation map to produce the 270 m high resolution map compatible with those available for South Korea. According to the final product, the spatial average precipitation for entire territory of North Korea is 1,196 mm for a climatological normal year (1971-2000) with standard deviation of 298 mm.
Proceedings of the Korean Vacuum Society Conference
/
2011.02a
/
pp.17-18
/
2011
Plasma in liquid phase has attracted great attention in the last few years by the wide domain of applications in material processing, decomposition of organic and inorganic chemical compounds and sterilization of water. The plasma in liquid is characterized by three main regions which interact each - other during the plasma operation: the liquid phase, which supply the plasma gas phase with various chemical compounds and ions, the plasma in the gas phase at atmospheric pressure and the interface between these two regions. The most complex region, but extremely interesting from the fundamental, chemical and physical processes which occur here, is the boundary between the liquid phase and the plasma gas phase. In our laboratory, plasma in liquid which behaves as a glow discharge type, is generated by using a bipolar pulsed power supply, with variable pulse width, in the range of 0.5~10 ${\mu}s$ and 10 to 30 kHz repetition rate. Plasma in water and other different solutions was characterized by electrical and optical measurements. Strong emissions of OH and H radicals dominate the optical spectra. Generally water with 500 ${\mu}S/cm$ conductivity has a breakdown voltage around 2 kV, depending on the pulse width and the repetition rate of the power supply. The characteristics of the plasma initiated in ultrapure water between pairs of different materials used for electrodes (W and Ta) were investigated by the time-resolved optical emission and the broad-band absorption spectroscopy. The deexcitation processes of the reactive species formed in the water plasma depend on the electrode material, but have been independent on the polarity of the applied voltage pulses. Recently, Coherent anti-Stokes Raman Spectroscopy method was employed to investigate the chemistry in the liquid phase and at the interface between the gas and the liquid phases of the solution plasma system. The use of the solution plasma allows rapid fabrication of the metal nanoparticles without being necessary the addition of different reducing agents, because plasma in the liquid phase provides a reaction field with a highly excited energy radicals. We successfully synthesized gold nanoparticles using a glow discharge in aqueous solution. Nanoparticles with an average size of less than 10 nm were obtained using chlorauric acid solutions as the metal source. Carbon/Pt hybrid nanostructures have been obtained by treating carbon balls, synthesized in a CVD chamber, with hexachloro- platinum acid in a solution plasma system. The solution plasma was successfully used to remove the template remained after the mesoporous silica synthesis. Surface functionalization of the carbon structures and the silica surface with different chemical groups and nanoparticles, was also performed by processing these materials in the liquid plasma.
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