• Title/Summary/Keyword: hybrid culture

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The Development and Application of a Training Base for the Installation and Adjustment of Photovoltaic Power Generation Systems

  • Chuanqing, SUN
    • International Journal of Advanced Culture Technology
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    • v.4 no.1
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    • pp.37-50
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    • 2016
  • In recent years, the development and application of green energy resources have attracted more and more /$^*$ 'tention of people. The training room presented here is focused on the terminal applications of a photovoltaic power generation system (PPGS). Through introducing the composition and the general design principles, we aimed at leading the students to master the fundamental skills required for its design, installation and construction. The training room consists of numerous platforms, such as: PPGS, Wind and Photovoltaic Hybrid Power Generation Systems, Wind Power Generation Equipments, Simulative Grid-Connected Power Generation System, Electronic Technology Application of New Energy, etc. This enables the students to obtain their project and professional skills training via assembling, adjusting, maintaining and inspecting, etc., various component parts of the photovoltaic and new energy power generation systems, to further grasp the fundamental and related theoretical knowledge, and to further reinforce their practical and operational skills, so as to improve their problem-analyzing and problem-solving abilities.

비친수성유기물질(HOC)로 오염된 토양의 정화를 위한 동전기-생물활성화공정의 개발

  • 양지원;김상준;박지연;이유진;기대정
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.09a
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    • pp.326-329
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    • 2003
  • When an electrokinetic process is applied to a HOC-contaminated soil, hybrid types combined with soil flushing, chemical oxidation, and bioremediation are generally used. Especially when the electrokinetic process is combined with bioremediation, the hybrid technology can solve several limits of bioremediation such as low microbial mobility, low soil temperature, and shortage of nutrients in subsurface circumstance. Because microbial surface is charged negatively, the microorganism moves from cathode to anode under electrical field. In this study, mixed culture mainly-consisted by Pseudomonas sp. was applied to remediate pentadecane-contaminated kaolinite with particle size less than 300${\mu}{\textrm}{m}$. This remediation system was named ‘electrokinetic bioaugmentation’ and consisted of model aquifer, electrode reservoirs, bioreactor, power supply, and pump. The mixed culture above 0.5 of optical density in bioreactor was supplied to two reservoirs and penetrated soil when the electric current was applied. To enhance the removal efficiency, the optimal medium composition, electric current, and voltage were investigated.

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Analysis of Odor Data Based on Mixed Neural Network of CNNs and LSTM Hybrid Model

  • Sang-Bum Kim;Sang-Hyun Lee
    • International Journal of Advanced Culture Technology
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    • v.11 no.4
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    • pp.464-469
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    • 2023
  • As modern society develops, the number of diseases caused by bad smells is increasing. As it can harm people's health, it is important to predict in advance the extent to which bad smells may occur, inform the public about this, and take preventive measures. In this paper, we propose a hybrid neural network structure of CNN and LSTM that can be used to detect or predict the occurrence of odors, which are most required in manufacturing or real life, using odor complex sensors. In addition, the proposed learning model uses a complex odor sensor to receive four types of data, including hydrogen sulfide, ammonia, benzene, and toluene, in real time, and applies this data to the inference model to detect and predict the odor state. The proposed model evaluated the prediction accuracy of the training model through performance indicators based on accuracy, and the evaluation results showed an average performance of more than 94%.

A Cascade-hybrid Recommendation Algorithm based on Collaborative Deep Learning Technique for Accuracy Improvement and Low Latency

  • Lee, Hyun-ho;Lee, Won-jin;Lee, Jae-dong
    • Journal of Korea Multimedia Society
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    • v.23 no.1
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    • pp.31-42
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    • 2020
  • During the 4th Industrial Revolution, service platforms utilizing diverse contents are emerging, and research on recommended systems that can be customized to users to provide quality service is being conducted. hybrid recommendation systems that provide high accuracy recommendations are being researched in various domains, and various filtering techniques, machine learning, and deep learning are being applied to recommended systems. However, in a recommended service environment where data must be analyzed and processed real time, the accuracy of the recommendation is important, but the computational speed is also very important. Due to high level of model complexity, a hybrid recommendation system or a Deep Learning-based recommendation system takes a long time to calculate. In this paper, a Cascade-hybrid recommended algorithm is proposed that can reduce the computational time while maintaining the accuracy of the recommendation. The proposed algorithm was designed to reduce the complexity of the model and minimize the computational speed while processing sequentially, rather than using existing weights or using a hybrid recommendation technique handled in parallel. Therefore, through the algorithms in this paper, contents can be analyzed and recommended effectively and real time through services such as SNS environments or shared economy platforms.

A Study on the Effect of Physical Stimuli on Bone Cell Differentiation Using a Hybrid Bioreactor (Hybrid Bioreactor를 이용한 물리적 자극에 대한 세포반응 연구)

  • 이창양;최귀원;홍대희
    • Journal of Biomedical Engineering Research
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    • v.25 no.4
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    • pp.309-314
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    • 2004
  • in this study, hybrid bioreactor was used to apply physical stimuli in cell culture. Effect of the applied physical stimuli on the growth and differentiation of MC3T3-El cell in a three-dimensional Chitosan scaffold were studied by using the hybrid bioreactor. The hybrid bioreactor for physical stimulus was specially designed to apply uniaxial cyclic compressive and shear strain. Physical stimulus was applied over a period of 14 days with 150 cycles per day at a frequency of 0.5Hz. Strain magnitude was 2.5% of the scaffold size. Control group and physically stimulated group of the MC3T3-El tell were incubated and harvested at the indicated times (Day 6, 8, 10, 12, 14). The total amount of protein, which obtained information of cell growth, was determined by Lowey method. Alkaline phosphatase activity was examined by ELISA. Physically stimulated group using the hybrid bioreactor was increased in alkaline phosphatase activity comparing with control group. The nodule formation and calcium deposit of the physical stimuli group which resulted in cell differentiation was faster than that of control group.

Expression of Green Fluorescent Protein in Both Spodoptera frugiperda Cells and Bombyx mori Larvae by Ac-Bm Hybrid Virus

  • Jin, Byung-Rae;Yoon, Hyung-Joo;Yun, Eun-Young;Kang, Seok-Woo;Cho, Eun-Sook;Kang, Seok-Kwon
    • The Journal of Korean Society of Virology
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    • v.28 no.3
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    • pp.225-232
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    • 1998
  • We have expressed GFP in Sf9 and Bm5 cells or Bombyx mori larvae by using Ac-Bm hybrid virus capable of replicating in both Bm5 and Sf9 cells. Genomic DNA of Ac-Bm hybrid virus expressing ${\beta}$-galactosidase was cotransfected with baculovirus transfer vector containing GFP gene, pBacPAK-GFP in Sf9 cells. The Ac-Bm hybrid virus harboring GFP was named as Ac-Bm hybrid virus-GFP. The Ac-Bm hybrid virus-GFP-infected insect cells were easily selected by detecting the emission of GFP from each well of cell culture dish on the UV illuminator. GFP produced by Ac-Bm hybrid virus-GFP in Sf9 and Bm5 cells or B. mori larvae was confirmed by SDS-PAGE and Western blot analysis using GFP antibody. In addition, B. mori larvae infected with Ac-Bm hybrid virus-GFP was apparently appeared fluorescence from the whole body at S days postinoculation. The fluorescence of GFP from the hemolymph and fat body of B. mori larvae infected with Ac-Bm hybrid virus-GFP was also observed by fluorescence microscope. In conclusion, our results demonstrated that in baculovirus expression vector system, use of Ac-Bm hybrid virus have an additional advantage of expanded host range for producing recombinant proteins.

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Fruit Yield and Quality Evaluation of Sweet Pepper (Capsicum annuum L.) F1 Hybrids Derived from Inbred Lines

  • Shrestha, Surendra Lal;Luitel, Binod Prasad;Lee, Taek Jong;Kang, Won Hee
    • Korean Journal of Breeding Science
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    • v.42 no.4
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    • pp.344-350
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    • 2010
  • Sweet pepper inbred lines (KNU1003, KNU1006, KNU1007, KNU1009, KNU1015, KNU1017 and KNU2006) developed at Kangwon National University (KNU) through conventional means, inbred lines (5AVS1, 5AVS2, 5AVS3, 5AVS5, 5AVS7 and 5AVS8) collected at Rural Development Administration (RDA) and inbred lines (SP12, SP27 and SP14) derived from anther culture were used as female parents and anther culture derived homozygous lines (SP9, SP10, SP14, SP24, SP25, SP27, SP30, SP32, SP34, SP38, SP43, SP45 and SP51) were used as male parents to produce $F_1$ hybrids. A total of 37 $F_1$ hybrids were evaluated for fruit yield and quality characters in summer season, 2007. Variation in fruit number, fruit weight, fruit yield per plant and fruit volume was observed among the $F_1$ hybrids. Superiority on yield over standard/commercial varieties were differed among $F_1$ hybrids. Hybrid $5AVS8{\times}SP45$ exhibited highest heterosis over Special (16.5%) and Fiesta (24.7%). Fruit quality characters (fruit length, fruit width, pericarp thickness, total soluble solid, fruit shape and fruit color) were varied among the $F_1$ hybrids. Fruit number, fruit weight and fruit volume per plant were correlated with fruit yield. Based on the standard heterosis expressed by the hybrids and quality characters evaluation, $KNU1017{\times}SP27$, $5AVS1{\times}SP43$, $5AVS5{\times}SP27$, $5AVS8{\times}SP45$, $SP12{\times}SP38$ and $SP27{\times}SP25$ hybrids were found to be superior over commercial cultivars and are selected. Inbred lines of these hybrid combinations can be used to produce $F_1$ hybrid seed for commercial production.

A Study on the Justification of Moral Responsibility in Hybrid Warfare: Focused on a Critical Evaluation of Jus In Bello (하이브리드 전쟁에서 도덕적 책임의 정당화에 관한연구: Jus In Bello에 대한 비판적 고찰을 중심으로)

  • Sangsu Kim;Hyunyoung Moon
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.5
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    • pp.57-63
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    • 2023
  • The purpose of this paper is to analyze the aspects of hybrid warfare and show the limits of jus in bello, a principle that evaluates the moral responsibility of the agents. As a theory of the morality of war presented in traditional warfare, the just war theory has been widely accepted. In particular, jus in bello offers the criteria of moral legitimacy regarding the actions of individuals participating in or involved in war. However, hybrid warfare, in which various aspects of warfare operate in a complex way, has obvious differences compared to traditional warfare, and this difference causes difficulties in determining the moral evaluation and attribution of responsibility for actions in war. In order to appropriately resolve the moral problems arising from hybrid warfare, it is necessary to clarify what the limitations of existing moral justification theories are. To this end, in this paper, we will first present a conceptual understanding of hybrid warfare, specify the differences from traditional warfare, and then show the limits of its application in hybrid warfare in light of the three principles that compose jus in bello.

Survival, Growth and Physiological Response of the Juvenile Hybrid Grouper (Epinephleus akaara♀×E. lanceolatus♂) Exposed to Different Water Salinity Levels (대왕붉바리 치어(Epinephleus akaara♀×E. lanceolatus♂)의 생존, 성장 및 생리학적 반응에 미치는 염분의 영향)

  • Shin, Yun Kyung;Choi, Young Jae;Gil, Hyun Woo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.4
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    • pp.426-433
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    • 2021
  • This study aimed to determine the appropriate salinity condition for optimal cultivation of the juvenile hybrid grouper Epinephleus akaara♀×E. lanceolatus♂ in conditions of decreasing salinity. The survival, growth, metabolic rate, stress, and histological response were investigated in juvenile hybrid grouper exposed to different salinities for 30 days. At 0 psu, the survival rate of the juvenile hybrid grouper was 60% on the 2nd day of exposure and all individuals died on the 3rd day of exposure. At salinities above 3 psu, all animals survived throughout the exposure period. Growth rate for body weight of the juvenile hybrid grouper was the highest in the control and the lowest at 10 psu. Plasma osmolality of the juvenile hybrid grouper exposed to different salinities was 313-355 mg Osmol/kg at salinities above 10 psu, and then decreased to 225-264 mg Osmol/kg at salinities below 5 psu. The oxygen consumption rate tended to decrease as the salinity decreased. Stress responses of the juvenile hybrid grouper were analyzed with decreasing salinity. Therefore, it is considered that a salinity of more than 20 psu is suitable for the culture of the juvenile hybrid grouper.

Response to Anther and Tissue Cultures of Corn, Pearl Millet and Buckwheat Genotypes (옥수수, 진주조, 메밀의 약 및 조직배양 반응)

  • Keun-Yong Park;Rae-Kyung Park;Byeong-Han Choi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.2
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    • pp.142-146
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    • 1989
  • Anther and/or tissue culture of cross pollinated crops would be very important because it can result in the direct use of haploids or doubled haploids for developing superior hybrids or varieties. The objective of the study was to investigate the response frequencies in anther and/or tissue-cultured hybrids of corn. pearl millet and buckwheat to identify agronomically acceptable germplasm of the crops. 27 crosses of corn inbred lines were evaluated by plating their anthers on N6. MS and Yu-Pei media. Two genotypes of FR1l41/FR16 hybrid cultured on N6 medium and Fla 2BT73/S6013 hybrid cultured on N6 medium responded with one anther producing calli when plated after 5$^{\circ}C$ low temperature treatment for one week. Immature embryos of corn hybrid Suwon 19 responded producing calli that were regenerated to plants at a 8.6 percent success rate. Of the 20 corn hybrids. immature tessels of FR1l41/FR16. B68/A1l6N//KS15. KS16/KS17. GA209/DB578 and SDB126/GA209 crosses responded at a relatively higher success rate producing calli that were regenerated to plants. In tissue culture of elongating culms of pearl millet x Napier grass interspecific hybrid. 2.5-4.0mm long pieces of the culm were good for callus induction resulting in higher success rate. The epicotyl of buckwheat was very good for tissue culture. and the node produced the plants regenerated directly without callus induction on the B5 medium containing I ppm BA and 0.05 ppm IBA. There were great differences in response to anther and/or tissue culture of corn, pearl millet and buckwheat due to genotype x medium and environment interactions.

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