• Toxicological Research
• /
• v.34 no.3
• /
• pp.191-197
• /
• 2018

In 2015, a candidate for the second national reference standard (NRS) of Gloydius snake venom was produced to replace the first NRS of Gloydius snake venom. In the present study, the potencies of the candidate were determined by a collaborative study, and the qualification of the candidate was estimated. The potencies of the candidate were determined by measuring the murine lethal titers and lapine hemorrhagic titers of venom against the regional working reference standard (RWRS) for antivenom using the methods described in the previous report for the first NRS of Gloydius snake venom. Three Korean facilities contributed data from a total of 30 independent assays. Subsequently, two foreign national control research laboratories contributed to this collaborative study. The results were calculated using the Reed-Muench method for lethality and determined using a mixed-effects model for hemorrhage. The general common potencies of the lethal and hemorrhagic titers were obtained from the results of the 30 tests performed at three Korean facilities. The results are expressed in micrograms for 1 test dose (TD) with a 95% confidence interval as follows: a lethal titer of $90.13{\mu}g/TD$ (95% confidence interval = $87.39{\sim}92.86{\mu}g$) and a hemorrhagic titer of $10.80{\mu}g/TD$ (95% confidence interval = $10.46{\sim}11.14{\mu}g$). In addition, the candidate preparation showed good quality evaluation according to the results of the quality estimation of the candidate and is judged to be suitable to serve as the Korean NRS for snake venom. In conclusion, the second NRS of Gloydius snake venom was established in this study and will be used for national quality control, including a national lot release test of Korean antivenom products.

• Journal of Preventive Medicine and Public Health
• /
• v.33 no.1
• /
• pp.17-24
• /
• 2000

Objectives : To propose the referential data to evaluate the health impacts of Vietnam veterans' children whose father were exposed to herbicides in Vietnam War. Methods : Vietnam veterans who visited to Pusan Veteran Hospital for medical care were recruited from April to October, 1998. They were 71 and asked about their own combat history, symptoms and illness, and health status of their 182 children. The informations were collected by direct and phone interview. Exposure estimation was also performed as exposure score depending on year and unit of participation, and personal episodes related to exposure to herbicide in the war. It classified into three groups; lower(<18.0), moderate(18-53), high$(\geq53)$ exposure group. Results : The mean age and the period into the combat of the veterans were 52.8 years and 15.0 months. The mean exposure score was $18.1{\pm}9.9$, and mainly distributed in lower (46.5%) and moderate(52.1%) exposure group. Most(90.1%) of them were diagnosed as sequelae(21 cases) and suspected sequelae(43 cases) of the herbicides by Korean veteran's hospital diagnostic criteria. The major sequelae was peripheral neuropathy 13 cases, chloracne 5 cases, and the major suspected sequelae was hypertension 20 cases, diabetes mellitus 18 cases, liver disease 12 cases, central neuropathy 11 cases, etc. About birth, 42.2% and 16.9% experienced spontaneous abortion and stillbirth, respectively. The mean exposure score was higher in stillbirth experience group(p<0.05). About half of the children(90 cases, 49.5%) hold the abnormal health status: those were skin pigmentation 38 cases, rash 23 cases, congenital anomaly 15 cases, general weakness 12 cases, purpura 8 cases, visual disturbance 8 cases, etc. These health problems had no association with father's exposure level(p>0.05). Conclusions : These results were depend on their own answers, and expectation for compensation did not excluded, therefore, this study may have limitations: inaccuracy of informations due to recall bias and response bias. Nevertheless, through this study, we could image the fundamental aspect for health impacts of Vietnam veterans' children for preparing the national control program and policy. A large scale epidemiologic study with valid exposure assessment on the health impacts of Vietnam veterans' children is recommneded.

• Korean Journal of Ecology and Environment
• /
• v.33 no.2 s.90
• /
• pp.136-144
• /
• 2000

M. aeruginosa isolated from Lake Paldang was cultured in CB medium, and then each wet plants put into the cultured medium at a rate of 0.5 g and 2.5 g wet wt/l. There was slight inhibition by the input of cattail and iris of each 0.5 g wet wt/l cultured medium, but showed no reduction in algal growth in other flasks. Among the applied plants, ginkgo, pine needles, big cone pine, waterreed and water chestnut had an effect on inhibition of algal growth at the input of 2.5 g wet wt/l. Plants which were dried for 3 days at $50^{\circ}C$ introduced into the testing flask for 10days cultured at dose rates of 2.5 g/l. When chlorophyll a concentration was remarkably high as $802.6\;{\mu}g/l$ after five days, there was noticeably less chlorophyll compared with control at a rate of 98% by big cone pine, 96% by ginkgo, 95% by pine needles and 86% by rice straw, respectively. To examine the effect of plant extracts on algal growth, big cone pine and water chestnut were put to the amount of 1.25 g liquid extracts/l. Chlorophyll a concentration and cell density decreased to the extent of average 43% as compared with the beginning of experiment, but when concentration of chlorophyll a increased a most high, the inhibition of algal growth by liquid extracts did not affect at all. When a quantity of plant equivalent to 2.5 g liquid extracts/l inhibited the growth of algae by 95% after nine days.

• Korean Journal of Food Science and Technology
• /
• v.17 no.2
• /
• pp.114-120
• /
• 1985

This study was carried out to investigate the storage stability of Yackwa (a Korean fried cake made from wheat flour, honey and 20% oil), with the variation in frying oils (soybean oil of fresh or heat-treated corn oil and rapeseed oil) and storage conditions (temperature, humidity and in dark or under fluorescent light) for 20 weeks period. In $5^{\circ}C$ storage, acid and peroxide values increased slowly, showing the peroxide value of 20 meq/kg after 13 weeks. In $40^{\circ}C$ storage, acid and peroxide values increased remarkably. The product became harder at storage humidity of 25% and softer by moistening at storage humidity of 80%. Acid and peroxide values of Yackwa from corn, rapeseed and soybean oils increased with storage period, to a different extent. The values of fluorescent groups, especially rapeseed oil group, were higher than those of control groups and the heat-treated oil groups were higher than those of fresh oil groups. Sensory scores for rancid flavor were gradually increased with the storage period in all groups. There was no significant difference with the kinds of frying oils, storage conditions and heat treatment. Correlation of sensory scores with acid values was positive throughout whole storage period, with low ${\gamma}-values$. Its correlation with peroxide values was positive up to 6th week but negative at 9th week of storage.

• Korean Journal of Food Science and Technology
• /
• v.23 no.2
• /
• pp.205-211
• /
• 1991

Ethanol extracts of amur cork, elm root, plantain and dandelion which are edible and can be mass produced at farm were examined their inhibitory activity against food spoilage microorganisms at their concentrations and the extracts were fractionated by some solvents with checking effective fractionate. Above $500{\sim}2000\;ppm$ of the extracts inhibited completely the test microorganisms with a few exception. One thousand ppm of amur cork exract inhibited B. cereus completely. L. mesenteroides by 500 ppm of amur cork and 2000 ppm of elm root and plantain showed a pretty good inhibition. The extracts which showed good inhibition to the test microorganisms were fractionated with chroloform, ethylacetate, butanol and water in order and the fractionates of butanol and chroloform showed comparetively higher inhibition than others generally. Inhibition rate of each fractionate were as follows ; B. cereus was inhibited completely at 500 ppm of chroloform fraction, and 1000 ppm of ethyl acetate and butanol and L. mesenteroides was 500 ppm of butanol fraction. P. fluorescens was inhibited partly by 500 ppm of butanol and ethyl acetate fraction.

• Journal of fish pathology
• /
• v.22 no.3
• /
• pp.275-282
• /
• 2009

In this study, effect of mating group, mating dam and mating sire was analyzed using olive flounder Paralichthys olivaceus challenged with Edwardsiella tarda. A challenge test was conducted using 1,708 olive flounder fingerlings obtained through $4{\times}4$ factorial cross from parents selected based on resistant degree. Regarding the means of resistant index, survival rate and days of survival by each mating group, the group(RR) produced by mating among resistant parents were 17.98, 13.77%, and 18.36 days, respectively; the group(SS) produced by mating among susceptible parents were 12.46, 2.71%, and 12.40 days, respectively. This study gives an indication that challenge test results may be successfully used as selection criteria for disease resistance to E. tarda in olive flounder, P. olivaceus.

• Applied Biological Chemistry
• /
• v.31 no.3
• /
• pp.226-233
• /
• 1988

Some physico-chemical properties and biological activities of new fungicide formulations were tested to investigate a feasibility of water-floating formulation development for sheath blight control in rice. Pencycuron[1-(4-chlorobenzyl)-1-cyclopentyl-3-phenylurea] and flutolanil$({\alpha},{\alpha},{\alpha}$-trifluoro-3'-isopropoxy-O-toluanilide) were chosen as toxicants for the formulations. Vegetable oil and surface active agents were used as a floating agent and spreader, respectively. All the formulations tested showed an excellent spreadability on the water having over $35cm^2/mg$ and were chemically stable, which the degradation rates of active ingredients were less than 10% after 12 weeks of storage at $50^{\circ}C$. Most of the applied test formulation of pencycuron was retained within 0.5cm of the surface paddy water, while that of flutolanil was vertically dispersed in the water. Inhibition activity of the tested pencycuron formulation on the sclerotia germination of the pathogen in paddy water was maintained over 30 days after the formulation treatment. Control effect of 4% pencycuron water-floating formulated with surface active agent of hydro-lipophyllic balance 4.3 on the disease in rice was equal to the reference fungicide(pencycuron 25% WP) when the former was treated one day before the transplantation of rice seedlings. Overflowing the submerged paddy water after the formulation treatment resulted in a deterious effect on the disease control.

• Journal of Fisheries and Marine Sciences Education
• /
• v.26 no.1
• /
• pp.72-80
• /
• 2014

Oxygen consumption (OC) of male black porgy Acanthopagrus schlegelii reared in freshwater (BFW) and seawater (BSW) during the reproductive period was $165.4{\pm}11.0$, $77.6{\pm}8.0$ mg $O_2/kg/h$ at $15^{\circ}C$, $186.2{\pm}13.1$, $133.4{\pm}6.7$ mg $O_2/kg/h$ at $20^{\circ}C$ and $267.9{\pm}19.1$, $198.6{\pm}8.3$ mg $O_2/kg/h$ at $25^{\circ}C$, respectively. During the non-reproductive period, it was shown as $174.0{\pm}7.0$, $85.6{\pm}5.5$ mg $O_2/kg/h$ at $15^{\circ}C$, $200.6{\pm}11.1$, $119.2{\pm}8.7$ mg $O_2/kg/h$ at $20^{\circ}C$ and $271.1{\pm}7.5$, $194.7{\pm}16.7$ mg $O_2/kg/h$ at $25^{\circ}C$, respectively. Thus, OC of BFW was higher than BSW both for non-reproductive and reproductive period. Also, OC increased in proportion to the rise in water temperature, and there was no difference of OC between BFW and BSW at each water temperature during the reproductive or non-reproductive period. OC of BFW and BSW showed clear circadian rhythms on photic conditions of reproductive and non-reproductive period, and the fish consumed more oxygen during the dark phase than for the light phase. In particular, OC of BFW during the reproductive period increased more sharply compared to non-reproductive period when the increase of water temperature was accompanied by the shift from dark to light phase. This implies that they react to the light more sensitively for reproductive period than for non-reproductive period.

• Pediatric Infection and Vaccine
• /
• v.13 no.2
• /
• pp.180-185
• /
• 2006

Invasive Pseudomonas infections most often occur in the immunocompromised patients and are associated with high mortality rate. Rarely this disease may develop in healthy infants and children. We report two cases of invasive Pseudomonas aeruginosa infections that were diagnosed in otherwise healthy infants. The first case was a previously healthy 5-month-old infant with ecthyma gangrenosum and septicemia. She presented with fever, swelling of left periorbital area and multiple erythronodular skin lesions. Each skin lesion formed a black eschar surrounded by an erythematous areola over time. Cultures of blood, urine and discharge from skin lesions grew P. aeruginosa. On the day of visit, she showed pancytopenia which was normalized after 10 days. The patient responded well to the management with ceftazidime and tobramycin. The other case was a previously healthy 9-month-old infant with community-acquired pneumonia. He was referred from an outside hospital with fever and cough. Chest x-ray revealed pneumonic infiltrations on both lower lungs with pleural effusion on the right side. Cultures of blood and pleural fluid grew P. aeruginosa. Chest CT performed on the ninth day demonstrated pneumatoceles, lung abscess and necrosis of lung parenchyma. He was managed with ceftazidime and amikacin for 50 days. No residual pulmonary complications were noted during the three month follow-up. Laboratory results to evaluate immunologic defects of phagocytic cells, complement components and T- and B-lymphocytes were all within normal range in both patients. It should be kept in mind that Pseudomonas can be, though uncommon, a cause of community-acquired invasive infections in the previously healthy infants.

• Korean Journal of Soil Science and Fertilizer
• /
• v.48 no.3
• /
• pp.194-204
• /
• 2015

Six study sites in Gumi, Goryeong in Gyeongbuk province and Naju in Jeonnam province were selected to investigate soil properties of poorly drained horizons in paddy soils. The horizons were re-established layers which were parent material layers originated from fluvial deposits. Topsoil layers were differentiated from piled parent materials while soil structure of the topsoil layer was massive with striated microstructure. Compaction at soil re-establishment and a lack of structure and aggregate development in these soils may cause the limitation of vertical water movement and result in poorly drained horizons. Soil samples were taken from paddy fields with top soils of sandy loam, silt loam and silty clay loam and re-established soils of coarse and fine texture. The samples were taken from each horizon for the analyses of soil chemical and mineral properties. Soils with re-established soils of coarse texture had greater amounts of sands from top soil texture distributions, while soils with fine texture had greater amounts of silts. Chemical properties of top soils were analyzed from rice cultivated soils at the time of re-establishments and one year after the re-establishments. The coarse texture of the re-established horizons decreased in EC values from 0.23 to $0.11(dS\;m^{-1})$, available phosphate values from 112 to $54(mg\;kg^{-1})$, and exchangeable Ca values from 6.6 to $4.9(cmol_c\;kg^{-1})$. On the other hand, soils with fine texture showed decrease only in pH and exchangeable Ca values. Especially, organic matter and available phosphate contents showed heterogeneous distributions from each horizon. This result may be caused by mixture of plough layer and subsurface layer during and consolidation. Hydraulic conductivity values were low at the boundaries of top soil and parent material layers except SL/coarse soil. Soil microstructure was massive structure without soil clods or pores and showed striated structure. Therefore, re-established paddy fields with fluvial deposits as parent material layers showed limited vertical movements of soil water because of occurrence of compacted layers and less-development of soil clods and aggregates.