• Fisheries and Aquatic Sciences
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• v.10 no.2
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• pp.60-67
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• 2007

Myostatin (MSTN; also known as GDF8) is a member of the transforming growth factor ${\beta}-superfamily$ of proteins. MSTN negatively regulates mammalian skeletal muscle growth and development by inhibiting myoblast proliferation. Mice and cattle possessing mutant MSTN alleles display a 'double muscling' phenotype characterized by extreme skeletal muscle hypertrophy and/or hyperplasia. We isolated the full-length cDNA of a novel MSTN gene from S. schlegeli muscle tissue and examined its expression pattern in various tissues. The full-length gene (GenBank DQ423474) consists of 1941bp with an open reading frame of 1134 bp, encoding 377 amino acids that show 62-92% amino acid similarity to other vertebrate MSTNs. The predicted protein contains a conserved proteolytic cleavage site (RXRR) and nine conserved cysteine residues at the C terminus. RT-PCR revealed that the unprocessed and prodomain myostatin mRNAs were predominantly present in muscle, with limited expression in other tissues. However, the mature myostatin mRNA was highly expressed in brain and muscle, intermediately expressed in the gills, intestine, heart, and kidney, and weakly expressed in the liver and spleen.

• Proceedings of the KSME Conference
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• 2003.11a
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• pp.1373-1377
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• 2003

Current prostheses for amputees are generally extrinsic wearing socket type that the coupling between body stump and appliance wraps the soft tissue and this structure causes several problems :applying direct weight to soft tissue such as skin and muscle, skin trouble of contacting area and pain. In this study, osseointegration implant is a method to directly connect prosthesis to the residual stump skeletal tissue of arm, finger and leg through surgical operation. Technology presented in this paper essentially solves the problems of pain and abnormal weight transfer system indicated above and recovers the functions of the amputated arm and leg. In this paper, implant shape was designed for the first step for the development of osseointegration implant and then we studied the possibility to apply this osseointegration implant to human body by performing implant insertion operation to beagle tibia for the clinical animal test and normal beagle's gait analysis was executed in order to quantitatively verify the beagle's skeletal functions after the implant insertion.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.64-64
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• 1995

Cholesteryl Ester Transfer Protein (CETP) mediates the transfer of cholesterol ester and triglyceride between high-density lipoprotein (HDL) and other low-density lipoproteins, therefore, it might influence HDL levels. The levels of HDL is closely related to the atherogenic diseases in human and there were several reports that the trasgenic mice expressing CETP had much worse atherosclerosis than non-expressing control one. Therefore, selective inhibitors of CETP have the potential to be used as antiatherosclerotic agents. Continued screening for potent inhibitors of CETP led to the isolation of Suberitenone B from marine sponge. Suberitenone B, sesterterpenoids of a new skeletal class have been isolated from the sponge Suberites sp. collected from King George Island the Antartic. The structure of the metabolite has been determined by NMR experiments and chemical methods.

• Journal of the Korean Society of Physical Medicine
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• v.18 no.3
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• pp.11-19
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• 2023

PURPOSE: This study evaluated the incidence of sarcopenic obesity (SO) and examined the specific risk factors in a community-dwelling middle-aged population of women. METHODS: The present study involved analyzing data from a cross-sectional study that included 1,693 community-dwelling women aged between 40 and 49 years. Various risk factors were investigated, including age, height, weight, body mass index, waist circumference, skeletal muscle mass index, smoking and drinking behaviors, systolic and diastolic blood pressure, fasting glucose levels, as well as triglyceride and cholesterol levels. To ensure the accuracy and validity of the results, a complex sampling technique was employed for data analysis. Each sample weight was calculated through a three-step process by estimating base weight, adjusting it for non-response, and modulating it for post-stratification. RESULTS: The incidence of SO was 4.26% (95% CI: 3.20-5.67%). The clinical risk factors for SO were age, height, weight, body mass index, waist circumference, skeletal muscle mass index, systolic blood pressure, diastolic blood pressure, and levels of fasting glucose, triglycerides, and total cholesterol (p < .05). CONCLUSION: This study explores the prevalence and risk factors of SO among community-dwelling women. It adds to the existing literature on SO and identifies potential risk factors in middle-aged women.

• Journal of the Korean Society of Physical Medicine
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• v.18 no.3
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• pp.21-30
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• 2023

PURPOSE: The present study evaluated the occurrence, somatometric, biochemical, and behavioral characteristics of young Korean sarcopenic males aged between 30 to 39 years. METHODS: The study involved a total of 1,546 participants. These individuals were divided into two distinct groups based on their skeletal muscle mass index. Of these, 49 participants were categorized into the sarcopenia group, while the remaining 1,497 individuals were placed in the normal group. The researchers analyzed the occurrence of sarcopenia, somatometric, biochemical, and behavioral characteristics such as height, weight, body mass index (BMI), waist circumference (WC), skeletal muscle mass index, fasting glucose (FG), triglyceride and total cholesterol (TC) levels, systolic (SBP) and diastolic blood pressure (DBP), and the drinking and smoking status. A complex sampling data analysis was performed. RESULTS: The weighted occurrence of sarcopenia was found to be 3.24%, (95% confidence interval (CI) of 2.39% to 4.36%). The somatometric contributing factors were height, BMI, and WC (p < .05). Triglyceride and TC levels exhibited statistically significant differences in the biochemical variables (p < .05). The behavioral characteristics, including the drinking and smoking status, had no statistical significance (p > .05). CONCLUSION: This study identified specific occurrences of sarcopenia and contributing factors in young Korean men.

• Journal of the Korean Society of Physical Medicine
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• v.18 no.2
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• pp.23-31
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• 2023

PURPOSE: This study examined the anthropometric and clinical risk factors and the prevalence of sarcopenia in women aged 40 to 49 years. METHODS: The study design is a cross-sectional research and a total of 2,055 participants were included. The participants were divided into two groups based on their skeletal muscle mass index score. One hundred and twenty-six individuals were assigned to a sarcopenia group, and 1,939 were assigned to a normal group. The following variables were analyzed: age, height, weight, body mass index, waist circumference, skeletal muscle mass index anthropometric measure, systolic blood pressure, diastolic blood pressure, blood laboratory tests, fasting glucose, triglyceride, total cholesterol, and smoking and drinking smoking statuses. RESULTS: The prevalence of sarcopenia was 6.5% (95% CI: 5.33-7.92). Anthropometric variables, such as height, BMI, and waist circumference, showed significance differences between the two groups (p < .05), except for weight variable (p > .05). In terms of blood pressure and blood lab tests, the systolic blood pressure, diastolic blood pressure, fasting glucose, triglyceride, and total cholesterol were all significant risk factors for sarcopenia in the two groups. (p < .05). CONCLUSION: This study identified risk factors and the prevalence of sarcopenia among community-dwelling middle-aged women.

• Journal of the Korean Society of Physical Medicine
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• v.18 no.4
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• pp.19-27
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• 2023

PURPOSE: This investigation in the study aimed to assess to determine proportion and susceptibility makers of sarcopenia in Korean younger female aged 30 to 39 years. METHODS: To address the complex sampling design of Korea National Health and Nutrition Examination Surveys, appropriate individual weights were incorporated into the analysis. The data employed a stratified, clustered, multistage probability sampling design. A total of 2,098 participants were enrolled and categorized into two groups based on their skeletal muscle mass index scores. One hundred and twenty-four individuals were placed in the sarcopenia group, while 2,024 were allocated to a normal group. The study examined various markers as variables, including age, height, weight, body mass index waist circumference, skeletal muscle mass index, systolic and diastolic blood pressure, fasting glucose, triglyceride, and total cholesterol levels, and smoking and drinking habits. RESULTS: The study found that proportion of sarcopenia in this population was 3.78% (CI: 2.89-4.94) in sarcopenia group and 96.22% (CI: 95.06-97.11) in normal with weighed values. Several susceptibilities including height, weight, BMI, waist circumference, diastolic blood pressure, and total cholesterol levels were risk factor for sarcopenia (p < .05), exhibited significant differences between the sarcopenia and normal groups. CONCLUSION: This investigation provides the proportion of sarcopenia and identifies relevant susceptibility markers among community dwelling younger women in Korea.

• Journal of the Korean Society of Physical Medicine
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• v.18 no.4
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• pp.57-66
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• 2023

PURPOSE: This research investigated clinical hazardous components and analyzed the proportion of sarcopenia among young Korean women. METHODS: The cross-sectional study included 1,236 women aged 20 to 29 years, categorized into two groups according to their skeletal muscle mass index (SMI). Of these, 20 participants were placed in the sarcopenia group, while 1,216 were included in the normal group. The analysis involved hazardous components including body dimensions, clinical indicators, and behavioral trait variables: height, weight, body mass index, waist circumference, skeletal muscle mass index, systolic and diastolic blood pressure, blood laboratory tests assessing fasting glucose, triglycerides, total cholesterol, as well as smoking habits and alcohol consumption. Complex sampling analysis was used to analyze the proportion and hazardous components of sarcopenia. RESULTS: The proportion of sarcopenia was at 1.76% (95% of CI: 1.08-2.83). Anthropometric measurements, such as height, BMI, and WC, exhibited significant differences between the groups (p < .05). However, there was no significant difference in weight (p > .05) between the two groups. Among the clinical indicators, SBP, DBP, FBG, serum triglycerides, and total TC found to be significant hazardous components for sarcopenia within both groups (p < .05). Smoking status as a behavioral trait was significant as well (p < .05), unlike alcohol consumption (p > .05). CONCLUSION: This study discerned both the proportion of sarcopenia and the hazardous components associated with it among community-dwelling women of a young age.

• Journal of Oral Medicine and Pain
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• v.21 no.2
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• pp.225-242
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• 1996

The author studied to determine the sex and age of 8 ancient human skeletal remains, which had excavated from ancient tombs located in Yeanri, Kimhae. Some kinds of personal identification methods their skulls and teeth were used for this study and the results were obtained as follows : 1. Sex determination was possible in ancient teeth from 4th to 5th century, using detection of X-Y homologous amelogenin gene by polymerase chain reaction. 2. DNA analysis proved that the materials examined were all male, but which always did not coincide with the results from other methods for sex determination including comparison of sexual differentiation of cranium and teeth and use of discriminant functions in the dental measurement. 3. There was little difference of the estimated ages between the methods by regression of pulp cavity and attrition in teeth. The ages from these two methods always did not coincide with ones from evaluation closure of cranial and palatal sutures. 4. Sex and age of the materials were determined as follows ; $\cdot$ Y9 was estimated to be male in early sixties. $\cdot$ Yl2 was estimated to be male in late twenties to early thirties. $\cdot$ Y37 was estimated to be male in early forties. $\cdot$ Y70 was estimated to be male in early sixties. $\cdot$ Y87 was estimated to be male in late forties. $\cdot$ Y109 was estimated to be male in early forties. $\cdot$ Yl29 was estimated to be male in late thirties to early forties. $\cdot$ Yl42 was estimated to be male in late fifties to early sixties.

• YAKHAK HOEJI
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• v.45 no.2
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• pp.190-204
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• 2001

This study was conducted to investigate for its effects on reproductive and developmental toxicity of recombinant human epidermal growth factor (rhEGF) in Sprague-Dawley rats. Male rats were administered rhEGF at doses of 1, 10, 100, and 1000$\mu$g/kg/day, respective1y, by subcutaneous injection from 63 days before and throughout to mating period until the day before sacrifice. Female rats were administered rhEGF at the same doses from 14 days before mating to day 20 of gestation or to day 21 of lactation. We examined the male and female fertility indices and maternal toxicity of F0 parental animals. Also, we examined the external, visceral, or skeletal malformation of fetuses, growth and development, behavior, and/or reproductive performance of F1 animals. At the highest dose (1,000 $\mu$g/kg), the mean body weights of F0 animals were significantly increased in males and females at 3 or 2 weeks after treatment, respective1y. No clinical signs and food intakes were observed at any time during the experimental period by rhEGF treatment. In autopsy examination, the relative and absolute liver weights significantly increased in both sexes of 1,000 $\mu$g/kg. At the highest dose (1,000 $\mu$g/kg), there was a statistically significant increase of pregnancy period and the number of dead fetuses. Moreover, significant increase of mean fetal body weight and decrease of number of live fetuses, which related to the difficult dilivery were observed in highest dose group. In Fl examination, no adverse effects on external, visceral, and skeletal malformation, physical and functional development, behavior or reproductive ability of Fl animals were observed in any group. Also, there was no significant difference between control and treated groups in copulation or fertility indices of Fl animals. These results indicate that rhEGF had no adverse effect on fertility and reproductive ability of Sprague-Dawley rats.