• Reproductive and Developmental Biology
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• 제35권2호
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• pp.185-190
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• 2011

To evaluate the effect of spermatozoa culture on glycosidase activity of frozen-thawed spermatozoa in human, the spermatozoa were treated experimentally and assayed for activities of ${\alpha}$-L-fucosidase, ${\alpha}$-D-mannosidase, ${\beta}$-D-galactosidase and N-acetyl-${\beta}$-D-glucosaminidase (${\beta}$-GlcNAc'ase). The ${\beta}$-GlcNAc'ase activity was at least two-folds higher than other glycosidases regardless of spermatozoa incubation (p<0.05). The spermatozoa motility was decreased with incubation periods, but no effects by different glycosidases on the changes of spermatozoa motility during the various periods of incubation. In all glycosidases, the spermatozoa-zona binding rates in spermatozoa without incubation were higher than in spermatozoa incubated for 2 h (p<0.05). ${\beta}$-GlcNAc'ase is present mainly in the plasma membrane of spermatozoa frozen-thawed in human. It was also shown that the glycosidase activity was increased in all glycosidases in spite of lower sperm-zona binding by spermatozoa incubation.

• Journal of Microbiology and Biotechnology
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• 제23권3호
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• pp.357-363
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• 2013

The identification of bacteriophage proteins on the surface of Lactobacillus rhamnosus Pen was performed by LC-MS/MS analysis. Among the identified proteins, we found a phage-derived major tail protein, two major head proteins, a portal protein, and a host specificity protein. Electron microscopy of a cell surface extract revealed the presence of phage particles in the analyzed samples. The partial sequence of genes encoding the major tail protein for all tested L. rhamnosus strains was determined with specific primers designed in this study. Next, RT-PCR analysis allowed detection of the expression of the major tail protein gene in L. rhamnosus strain Pen at all stages of bacterial growth. The transcription of genes encoding the major tail protein was also proved for other L. rhamnosus strains used in this study. The present work demonstrates the spontanous release of prophage-encoded particles by a commercial probiotic L. rhamnosus strain, which did not significantly affect the bacterial growth of the analyzed strain.

• 아동학회지
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• 제10권2호
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• pp.1-18
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• 1989

This documentary study explored the stages of human development and education in the Choson Dynasty with the use of "Non Eu, Ye Gi, So Hak, Tae Gyo Sin Gi, and Dong Yi Bo Gam. The findings were compared with Western writings of a comparable period. It was found that education in the Choson Dynasty was largely concerned with mental attitude and conduct in ordinary life (education for manners and living). Education claimed to stand for unity of knowledge and behavior. It was founded on a Confucian view of education with a saint as the ideal model to be emulated. In the early years, home influence was emphasized. This was later replaced by institutional education. Additional education frequently extended to ten years and even forty or more years for the development of leaders. Based on the above findings. It was concluded that (1) the Western stages of human development exemplified by the Puritans and by Rousseau were concerned only with progressive changes from the postnatal stage to adolescence but the stages or the Choson Dynasty applied to the entire life span, from prenatal life through old age: (2) the developmental stages of males and females were defined differently as a result of differences in educational contents and sex-role distinctions: (3) life span educational objectives were Confucian and the preparatory stages were long coming to fruition in late senescence: and (4) although education was centered on manners and based on Confucianism, much of human development in the Choson Dynasty corresponds to that of modern times.

• Nuclear Engineering and Technology
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• 제29권1호
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• pp.78-84
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• 1997

The specific purpose of this study is to develop the numerical guide for the cost-benefit analysis of ORE ($/person-Sv reduction) to meet the criterion of ALARA in the design stage of the KNGR. In deriving the guide, the risk factor which is defined by the risk to unit collective radiation exposure dose (deaths/person-Sv) and the monetary value of human life ($/death) are required. The risk factor has been estimated from various clinical data accumulated for a number of years and continuously modified. And the monetary value of human life is usually quantified using the human capital approach. In this study, the risk to radiation exposure perceived by a group of people is investigated through an extensive poll survey conducted among university students in order to modify the existing risk factor for radiation exposure. And in evaluating the monetary value of human life, the QOL factor is introduced in order to incorporate the degree of public welfare or quality of life. As a result of study, a value within the range of 151, 000~172, 000 dollars per person-Sv reduction is recommended as the appropriate interim numerical guide for cost-benefit analysis of ORE to meet the criterion of ALARA in the design stage of the KNGR. A poll survey was also conducted in order to see whether the public acceptance cost of nuclear power should be incorporated in developing the guide, and the result of study shooed that such a cost does not need to be considered.

• 문화기술의 융합
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• 제8권5호
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• pp.193-199
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• 2022

본 연구는 노인의 인적자본이 생활만족도에 미치는 영향을 파악하고자 하였다. 연구대상자는 국민노후보장패널조사 8차 부가조사 자료를 활용하였으며 대상자는 1,987명이었다. SPSS 20.0을 이용하여 기술통계, t-test, ANOVA, 회귀분석을 실시하였다. 그 결과, 성별, 연령, 교육수준, 경제활동 상태, 세대구성 형태, 배우자유무, 운동 형태가 생활만족도에 유의한 차이를 보였다. 생활만족도와 건강상태는 가장 높은 정(+)의 상관관계를 나타냈고, 월평균소득과 교육수준은 가장 높은 부(-)의 상관관계를 나타냈다. 생활만족도에 유의한 영향을 미치는 인적자본으로는 남성보다는 여성이, 비취업자보다는 취업자가, 부부세대의 가족형태인 경우에, 건강할수록, 월평균 소득이 높을수록, 규칙적인 운동을 할수록 생활만족도가 상대적으로 높았다.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2006년도 제47회 학술심포지움 및 추계국제학술대회
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• pp.44.2-44.2
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• 2006

• 생명과학회지
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• 제23권1호
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• pp.84-94
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• 2013

커피와 유사한 향미기를 갖는 녹차(Coffee-like green tea: CLGT)를 제조하고 이의 인체 유방암세포 주 MCF-7, 인체 전립선암세포 주 PC-3, 인체 신경모세포 주 SK-N-SH 및 쥐 심근세포 주 H9c2에 대한 세포독성을 연구하였다. 녹차엽(GTL)을 $240^{\circ}C$에서 1시간 roasting한 녹차가 커피와 가장 유사한 향미를 나타내었다. CLGT는 GTL과 비교하여 인체 암세포 및 정상세포와 쥐 심근세포에 대한 세포독성 차이가 없었다. GTL의 roasting은 ECG 등의 catechin 성분과 total protein은 유의성 있게 감소시켰지만, total phenol 및 total sugar는 유의성 있는 감소시키지 않아, 이와 같은 화합물 조성의 변화가 GTL에 커피유사 향미를 제공하였을 것이다. 따라서 이들 결과는 CLGT이 인체에 안전하여 커피 대용품으로 사용할 수 있음을 의미한다.

• 한국미생물·생명공학회지
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• 제36권3호
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• pp.221-226
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• 2008

효모에서 사람의 H-, L-ferritin을 생산하기 위해서, 기존에 복제된 vector를 사용하였으며,단백질을 발현하기 위해서 각각의 증폭된 ferritin 유전자를 GALI promoter에 의해 조절되는 pYES2.1/V5-His-TOPO 효모 발현 vector에 삽입하였다. Western blot 분석을 통해서 사람의 H-, L-ferritin subunits을 함유한 재조합 효모에서 사람의 ferritin이 발현된 것을 확인할 수 있었다. 또한 Atomic absorption spectrometry(AAS) 분석을 통해서 형질변환된 효모의 철 함유량이 대조군과 비교하여 $1.6{\sim}l.8$배 증가한 것을 확인하였다. 향후 ferritin이 함유된 형질변환 효모를 사용하여 잠재적으로 철이 강화된 영양성분을 기능성 식품과 사료에 이용할 수 있을 것이다.

• BMB Reports
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• 제53권2호
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• pp.118-123
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• 2020

Cardiac regeneration with adult stem-cell (ASC) therapy is a promising field to address advanced cardiovascular diseases. In addition, extracellular vesicles (EVs) from ASCs have been implicated in acting as paracrine factors to improve cardiac functions in ASC therapy. In our work, we isolated human cardiac mesenchymal stromal cells (h-CMSCs) by means of three-dimensional organ culture (3D culture) during ex vivo expansion of cardiac tissue, to compare the functional efficacy with human bone-marrow derived mesenchymal stem cells (h-BM-MSCs), one of the actively studied ASCs. We characterized the h-CMSCs as CD90^low, c-kit^negative, CD105^positive phenotype and these cells express NANOG, SOX2, and GATA4. To identify the more effective type of EVs for angiogenesis among the different sources of ASCs, we isolated EVs which were derived from CMSCs with either normoxic or hypoxic condition and BM-MSCs. Our in vitro tube-formation results demonstrated that the angiogenic effects of EVs from hypoxia-treated CMSCs (CMSC-Hpx EVs) were greater than the well-known effects of EVs from BM-MSCs (BM-MSC EVs), and these were even comparable to human vascular endothelial growth factor (hVEGF), a potent angiogenic factor. Therefore, we present here that CD90^lowc-kit^negativeCD105^positive CMSCs under hypoxic conditions secrete functionally superior EVs for in vitro angiogenesis. Our findings will allow more insights on understanding myocardial repair.

• Nutrition Research and Practice
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• 제14권2호
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• pp.167-174
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• 2020

BACKGROUND/OBJECTIVES: With the advances in technologies, self-service kiosks at foodservice operations are becoming a new way of service provision. This study examined the relationships among the menu information quality, nutrition information quality, technology acceptance characteristics, and customer behavioral intention toward the kiosks in fast food restaurants. SUBJECTS/METHODS: A survey with a self-administered method was distributed online and offline. The sample consisted of customers who had used the kiosks at fast food restaurants in the last six months prior to the survey. The study hypotheses were tested by applying structural equation modeling. RESULTS: Structural equation modeling revealed the positive impacts of menu information quality and nutrition information quality, technology acceptance characteristics, and behavioral intention toward kiosks at fast food restaurants. On the other hand, one hypothesis (Hypothesis 4) on the impact of nutrition information quality on the perceived usefulness was rejected. CONCLUSION: The study is the first to investigate nutrition and menu information at foodservice kiosks and relate them to technology acceptance. The study is very timely and adequate in the time of the 4^th industrial revolution. The critical importance of the presentation of nutrition information and menu information at the kiosks at fast food restaurants was verified. The academic and industrial implications of the study findings were discussed.