• Food Science of Animal Resources
• /
• v.30 no.1
• /
• pp.36-41
• /
• 2010

The Hen's egg is widely used in many processed foods as an ingredient and is one of the most prevalent food allergens in children. To detect egg proteins in processed foods, we developed a competitive indirect enzyme-linked immunosorbent assay (ciELISA) using an anti-ovomucoid (OM) antibody, which was produced by immunization of rabbits with OM, the most heat-stable component of the egg proteins. The detection limit of this quantitative assay system was 30 ng/mL. Cross-reactivity of the anti-OM antibody toward OM, ovalbumin, skim milk, casein, whey protein isolate, and isolated soy protein was 100, 0.4, 0.2, 0.04, 0, and 0%, respectively. In the spike test of egg white powder in milk replacer, commercial sausage, and in-house sausage, the assay recoveries ($mean{\pm}SD$) were $129{\pm}13.7%$, $73.9{\pm}12.5%$, and $65.5{\pm}13.6%$, respectively. When egg white in a commercial crab meat analog and sausage was determined by ciELISA, the assay recovery was found to be 108% and 127%, respectively. The combined results of this study indicate that this novel ciELISA for OM detection could be applied for the quantification of hen's egg proteins in processed foods.

• Fire Science and Engineering
• /
• v.23 no.2
• /
• pp.1-5
• /
• 2009

We studies wish to investigated establishment form of cabinet board, and confirm possibility of electrical disaster prevention through reappearance experiment of odor detection system. Established breaker consists of MCCB, RCD order in cabinet board for house, but industry is used together with. When imposed shock using shaker to terminal block that contact becomes in appropriate, flame was made sure. According to result that experiment attaching odor capsule in terminal block, capsule commissioned exactly by occurred heat. According to establishment position of sensor, difference of inspection time was about 10 seconds. Estimate odor inspection system by thing which electrical device accident prevention is available. When there is abnormal generated heat in connection of electric wire, accident prevention estimates that is possible by giving an alarm state of overheat to administrator.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2014.02a
• /
• pp.419-419
• /
• 2014

Interdigitated nanogap device (IND) is an attractive tool for biomolecular detection due to its huge on-off signal ratio, great tolerance to the variation in biochemical environment, and relatively simple implementation processes. Here, we report on the IND-based detection of Influneza A virus by sandwich immunoassay. The INEs were fabricated by photo lithography followed by the in-house chemical lithographic technique for the narrowing the initial gap distance. The surface of the silicon oxide between the two gold electrodes was chemically modified to immobilize primary antibodies for the immuno-specific interaction with the influenza A virus antigen. After immersing the functionalized-IND into the sample solution containing the influenza A virus, the device was exposed to the secondary antibody conjugated Au nanoparticles (Au NPs). The INDs showed a huge jump in the electric conductance when the sample solution contained the influenza A virus of the concentration as low as 10 ng/mL. We hope that this IND-based sensing can be applied to the development of simple and reliable diagnostic means of influenza viruses.

• Journal of Institute of Control, Robotics and Systems
• /
• v.14 no.3
• /
• pp.248-253
• /
• 2008

This paper presents a sensing system for smart home which can detect an location transition events such as entrance/exit of a member and identify the user in a group at the same time. The proposed system is compose of two sub-systems; a wireless sensor network system and a database server system. The wireless sensing system is designed as a star network where each of sensing modules with ultrasonic sensors and a Bluetooth RF module connect to a central receiver called Bluetooth access point. We propose a method to discriminate a user by measuring the height of the user. The differences in the height of users is a key feature for discrimination. At the same time, the each sensing module can recognize whether the user goes into or out a room by using two ultrasonic sensors. The server subsystem is a sort of data logging system which read the detected event from the access point and then write it into a database system. The database system could provide the location transition information to wide range of context-aware applications for smart home easily and conveniently. We evaluate the developed method with experiments for three subjects in a family with the installation of the developed system into a real house.

• Journal of Digital Convergence
• /
• v.10 no.8
• /
• pp.225-231
• /
• 2012

In this paper, we implement a wireless home monitoring system using physical computing platform with the open source type for house environment management. Sensors for temperature, optics and ultrasonic waves are used for home environment elements detection and control for the home safety. To obtain information related with home and transmit it to RIA Windows server system, XBee wireless communication system is used and the transmitted information is saved in mySQL database for history management. The possibility of prevention against fire disaster of a house situated in a dense buildings area and highly populated environments is suggested by adopting the proposed system.

• Parasites, Hosts and Diseases
• /
• v.54 no.1
• /
• pp.1-8
• /
• 2016

Laboratory workers, in resource-poor countries, still consider PCR detection of Giardia lamblia more costly and more time-consuming than the classical parasitological techniques. Based on 2 published primers, an in-house one-round touchdown PCR-RFLP assay was developed. The assay was validated with an internal amplification control included in reactions. Performance of the assay was assessed with DNA samples of various purities, 91 control fecal samples with various parasite load, and 472 samples of unknown results. Two cysts per reaction were enough for PCR detection by the assay with exhibited specificity (Sp) and sensitivity (Se) of 100% and 93%, respectively. Taking a published small subunit rRNA reference PCR test results (6%; 29/472) as a nominated gold standard, G. lamblia was identified in 5.9% (28/472), 5.2%, (25/472), and 3.6% (17/472) by PCR assay, $RIDA^{(R)}$ Quick Giardia antigen detection test (R-Biopharm, Darmstadt, Germany), and iodine-stained smear microscopy, respectively. The percent agreements (kappa values) of 99.7% (0.745), 98.9% (0.900), and 97.7% (0.981) were exhibited between the assay results and that of the reference PCR, immunoassay, and microscopy, respectively. Restriction digestion of the 28 Giardia-positive samples revealed genotype A pattern in 12 and genotype B profile in 16 samples. The PCR assay with the described format and exhibited performance has a great potential to be adopted in basic clinical laboratories as a detection tool for G. lamblia especially in asymptomatic infections. This potential is increased more in particular situations where identification of the parasite genotype represents a major requirement as in epidemiological studies and infection outbreaks.

• Journal of Internet Computing and Services
• /
• v.21 no.5
• /
• pp.109-118
• /
• 2020

Recently, people are spending a lot of time inside their homes because of various diseases. It is difficult to ask others for help in the case of a single-person household that is injured in the house or infected with a disease and needs help from others. In this study, an algorithm is proposed to detect emergency event, which are situations in which single-person households need help from others, such as injuries or disease infections, in their homes. It proposes vision pattern detection algorithms using home CCTVs, audio pattern detection algorithms using artificial intelligence speakers, activity pattern detection algorithms using acceleration sensors in smartphones, and dust pattern detection algorithms using air purifiers. However, if it is difficult to use due to security issues of home CCTVs, it proposes a fusion method combining audio, activity and dust pattern sensors. Each algorithm collected data through YouTube and experiments to measure accuracy.

• Journal of Institute of Control, Robotics and Systems
• /
• v.11 no.12
• /
• pp.1011-1019
• /
• 2005

In this paper, Ubiquitous Home Mess-Cleanup Robot(UHMR), which has a practical function of the automatic mess-cleanup, is developed. The vacuum-cleaner had made the burden of house chore lighten but the operation labour of a vacuum-cleaner had been so severe. Recently, the cleaning robot was producted to perfectly solve the cleaning labour of a house but it also was not successful because it still had a problem of mess-cleaning, which was the clean-up of big trash and the arrangement of newspapers, clothes, etc. The cleaning robot is to just vacuum dust and small trash but has no function to arrange and take away before the automatic vacuum-cleaning. For this reason, the market for the cleaning robot is not yet built up. So, we need a design method and technological algorithm of new automatic machine to solve the problem of mess-cleanup in house. It needs functions of agile automatic navigation, novel manipulation system for mess-cleanup. The automatic navigation system has to be controlled for the full scanning of living room, to recognize the absolute position and orientation of tile self, the precise tracking of the desired path, and to distinguish the mess object to clean-up from obstacle object to just avoid. The manipulate,, which is not needed in the vacuum-cleaning robot, must have the functions, how to distinguish big trash to clean from mess objects to arrange, how to grasp in according to the form of mess objects, how to move to the destination in according to mess objects and arrange them. We use the RFID system to solve the problems in this paper and propose the reading algorithm of RFID tags installed in indoor objects and environments. Then, it should be an intelligent system so that the mess cleaning task can be autonomously performed in a wide variety of situations and environments. It needs to also has the entertainment functions for the good communication between the human and UHMR. Finally, the good performance of the designed UHMR is confirmed through the results of the mess clean-up and arrangement.

• 제어로봇시스템학회:학술대회논문집
• /
• 2005.06a
• /
• pp.1612-1616
• /
• 2005

In this paper, a Home Mess-Cleanup Robot(HMR), which has a practical function of the automatic mess-cleanup, is developed. The vacuum-cleaner had made the burden of house chore lighten but the operation labour of a vacuum-cleaner had been so severe. Recently, the cleaning robot was producted to perfectly solve the cleaning labour of a house but it also was not successful because it still had a problem of mess-cleaning, which was the clean-up of big trash and the arrangement of newspapers, clothes, etc. The cleaning robot is to just vacuum dust and small trash but has no function to arrange and take away before the automatic vacuum-cleaning. For this reason, the market for the cleaning robot is not yet built up. So, we need a design method and technological algorithm of new automatic machine to solve the problem of mess-cleanup in house. It needs functions of agile automatic navigation, novel manipulation system for mess-cleanup. The automatic navigation system has to be controlled for the full scanning of living room, to recognize the absolute position and orientation of the self, the precise tracking of the desired path, and to distinguish the mess object to clean-up from obstacle object to just avoid. The manipulator, which is not needed in the vacuum-cleaning robot, must have the functions, how to distinguish big trash to clean from mess objects to arrange, how to grasp in according to the form of mess objects, how to move to the destination in according to mess objects and arrange them. We use the RFID system to solve the problems in this paper and propose the reading algorithm of RFID tags installed in indoor objects and environments. Then, it should be an intelligent system so that the mess cleaning task can be autonomously performed in a wide variety of situations and environments. It needs to also has the entertainment functions for the good communication between the human and HMR. Finally, the good performance of the designed HMR is confirmed through the results of the mess clean-up and arrangement.

• Journal of Korean Society of Occupational and Environmental Hygiene
• /
• v.21 no.4
• /
• pp.201-208
• /
• 2011

Objectives: According to the compliance of the asbestos-related regulation, every building has to be inspected for asbestos presence before its abatement work. This study was performed for identifying the types and contents of asbestos in building bulk samples. Materials and Methods: Bulk samples were collected during the asbestos inspection in 2010. We grouped the bulk samples into the regulated asbestos containing materials(RACM), presumed asbestos containing materials(PACM), and construction products. Additionally, the types of asbestos in all bulk samples were identified by polarization microscopy(PLM). Results: The RACMs were from building, house, pipe and facility. The RACMs were found mainly building (72.1%) and house (93.7%). The contents of chrysotile in building, house and facility were 66.9% (1-90%), 89.7% (2-90%) and 11.0% (2-90%), respectively. PACMs were surfacing material, thermal system insulation (TSI), and miscellaneous material. The miscellaneous materials that showed a high detection rate (79.2%) were ceiling, roofing and wall materials. Among them, the roofing materials had high chrysotile content(9.7%, 2-21%), followed by wall (8.7%, 2-21%) and ceiling (3.4%, 1-17%). In the construction products, asbestos was found mainly in slate (92.6%, 2-21%), including chrysotile. The slate had high asbestos content (9.7%, 2-21%), followed by cement flat board (8.7%, 2-19%) and textile (3.4%, 1-17%) Conclusions: Utilizing these results, it would be contributed to construct a useful ACM database and prevent from asbestos exposure to workers in the asbestos abatement and maintenance works.