• 한국축산식품학회지
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• 제30권1호
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• pp.36-41
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• 2010

The Hen's egg is widely used in many processed foods as an ingredient and is one of the most prevalent food allergens in children. To detect egg proteins in processed foods, we developed a competitive indirect enzyme-linked immunosorbent assay (ciELISA) using an anti-ovomucoid (OM) antibody, which was produced by immunization of rabbits with OM, the most heat-stable component of the egg proteins. The detection limit of this quantitative assay system was 30 ng/mL. Cross-reactivity of the anti-OM antibody toward OM, ovalbumin, skim milk, casein, whey protein isolate, and isolated soy protein was 100, 0.4, 0.2, 0.04, 0, and 0%, respectively. In the spike test of egg white powder in milk replacer, commercial sausage, and in-house sausage, the assay recoveries ($mean{\pm}SD$) were $129{\pm}13.7%$, $73.9{\pm}12.5%$, and $65.5{\pm}13.6%$, respectively. When egg white in a commercial crab meat analog and sausage was determined by ciELISA, the assay recovery was found to be 108% and 127%, respectively. The combined results of this study indicate that this novel ciELISA for OM detection could be applied for the quantification of hen's egg proteins in processed foods.

• 한국화재소방학회논문지
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• 제23권2호
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• pp.1-5
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• 2009

본 연구에서는 분전반의 설치 형태를 조사하였고, 향 검지 시스템의 재현 실험을 통해서 전기 재해 예방의 가능성을 확인하고자 한다. 주택용 분전반 내에 설치된 차단기는 MCCB, RCD 순서로 되어 있으나, 산업용은 혼용되고 있다. 접속이 부적절하게 된 단자대에 가진기를 이용하여 진동을 가했을 때 불꽃이 확인되었다. 단자대에 향 캡슐을 부착하여 실험한 결과, 발생한 열에 의해 캡슐이 정확하게 작동되었다. 센서의 설치 위치에 따라 검지 시간의 차이는 약 10초 이었다. 향 검지 시스템은 전기 설비 사고 예방이 가능한 것으로 판단된다. 전선의 접속부에서 이상 발열이 있을 때 과열의 상태를 관리자에게 경보해 줌으로써 사고 예방이 가능할 것으로 판단된다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.419-419
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• 2014

Interdigitated nanogap device (IND) is an attractive tool for biomolecular detection due to its huge on-off signal ratio, great tolerance to the variation in biochemical environment, and relatively simple implementation processes. Here, we report on the IND-based detection of Influneza A virus by sandwich immunoassay. The INEs were fabricated by photo lithography followed by the in-house chemical lithographic technique for the narrowing the initial gap distance. The surface of the silicon oxide between the two gold electrodes was chemically modified to immobilize primary antibodies for the immuno-specific interaction with the influenza A virus antigen. After immersing the functionalized-IND into the sample solution containing the influenza A virus, the device was exposed to the secondary antibody conjugated Au nanoparticles (Au NPs). The INDs showed a huge jump in the electric conductance when the sample solution contained the influenza A virus of the concentration as low as 10 ng/mL. We hope that this IND-based sensing can be applied to the development of simple and reliable diagnostic means of influenza viruses.

• 제어로봇시스템학회논문지
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• 제14권3호
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• pp.248-253
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• 2008

This paper presents a sensing system for smart home which can detect an location transition events such as entrance/exit of a member and identify the user in a group at the same time. The proposed system is compose of two sub-systems; a wireless sensor network system and a database server system. The wireless sensing system is designed as a star network where each of sensing modules with ultrasonic sensors and a Bluetooth RF module connect to a central receiver called Bluetooth access point. We propose a method to discriminate a user by measuring the height of the user. The differences in the height of users is a key feature for discrimination. At the same time, the each sensing module can recognize whether the user goes into or out a room by using two ultrasonic sensors. The server subsystem is a sort of data logging system which read the detected event from the access point and then write it into a database system. The database system could provide the location transition information to wide range of context-aware applications for smart home easily and conveniently. We evaluate the developed method with experiments for three subjects in a family with the installation of the developed system into a real house.

• 디지털융복합연구
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• 제10권8호
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• pp.225-231
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• 2012

본 논문에서는 자택 환경 관리를 위한 오픈소스 유형의 피지컬 컴퓨팅 플랫폼을 활용한 무선 자택관리 시스템을 구현한다. 화재 등의 재난 방지를 위한 자택 환경 요소 감지 및 제어를 고려하여 온도, 조도, 초음파 센서를 적용하였으며, 정보 수집 및 관리를 위해 RIA 윈도우즈 서버 시스템과의 연동은 XBee 무선 통신을 통하여 전송하고, 전송된 정보는 mySQL데이터베이스에 저장하여 히스토리 관리가 가능하도록 하였다. 공간과 사람이 밀집된 열악한 상태에 있는 자택환경의 관리 및 보안에 제안된 시스템을 적용함으로써 재해와 재난에 대비하고 화재 등의 재난을 억제할 수 있는 가능성을 제시한다.

• Parasites, Hosts and Diseases
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• 제54권1호
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• pp.1-8
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• 2016

Laboratory workers, in resource-poor countries, still consider PCR detection of Giardia lamblia more costly and more time-consuming than the classical parasitological techniques. Based on 2 published primers, an in-house one-round touchdown PCR-RFLP assay was developed. The assay was validated with an internal amplification control included in reactions. Performance of the assay was assessed with DNA samples of various purities, 91 control fecal samples with various parasite load, and 472 samples of unknown results. Two cysts per reaction were enough for PCR detection by the assay with exhibited specificity (Sp) and sensitivity (Se) of 100% and 93%, respectively. Taking a published small subunit rRNA reference PCR test results (6%; 29/472) as a nominated gold standard, G. lamblia was identified in 5.9% (28/472), 5.2%, (25/472), and 3.6% (17/472) by PCR assay, $RIDA^{(R)}$ Quick Giardia antigen detection test (R-Biopharm, Darmstadt, Germany), and iodine-stained smear microscopy, respectively. The percent agreements (kappa values) of 99.7% (0.745), 98.9% (0.900), and 97.7% (0.981) were exhibited between the assay results and that of the reference PCR, immunoassay, and microscopy, respectively. Restriction digestion of the 28 Giardia-positive samples revealed genotype A pattern in 12 and genotype B profile in 16 samples. The PCR assay with the described format and exhibited performance has a great potential to be adopted in basic clinical laboratories as a detection tool for G. lamblia especially in asymptomatic infections. This potential is increased more in particular situations where identification of the parasite genotype represents a major requirement as in epidemiological studies and infection outbreaks.

• 인터넷정보학회논문지
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• 제21권5호
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• pp.109-118
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• 2020

최근 다양한 질병 때문에 사람들은 집 안에서 많은 시간을 보내고 있다. 집 안에서 다치거나 질병에 감염되어 타인의 도움이 필요한 1인 가구의 경우 타인에게 도움을 요청하기 어렵다. 본 연구에서는 1인 가구가 집 안에서 부상이나 질병 감염 등 타인의 도움이 필요로 하는 상황인 이상 징후를 탐지하기 위한 알고리즘을 제안한다. 홈 CCTV를 이용한 영상 패턴 탐지 알고리즘과 인공지능 스피커 등을 이용한 음성 패턴 탐지 알고리즘, 스마트폰의 가속도 센서를 이용한 활동 패턴 탐지 알고리즘, 공기청정기 등을 이용한 먼지 패턴 탐지 알고리즘을 제안한다. 하지만, 홈 CCTV의 보안 문제로 사용하기 어려울 경우 음성, 활동, 먼지 패턴 센서를 결합한 융합 방식을 제안한다. 각 알고리즘은 유튜브와 실험을 통해 데이터를 수집하여 정확도를 측정했다.

• 제어로봇시스템학회논문지
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• 제11권12호
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• pp.1011-1019
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• 2005

In this paper, Ubiquitous Home Mess-Cleanup Robot(UHMR), which has a practical function of the automatic mess-cleanup, is developed. The vacuum-cleaner had made the burden of house chore lighten but the operation labour of a vacuum-cleaner had been so severe. Recently, the cleaning robot was producted to perfectly solve the cleaning labour of a house but it also was not successful because it still had a problem of mess-cleaning, which was the clean-up of big trash and the arrangement of newspapers, clothes, etc. The cleaning robot is to just vacuum dust and small trash but has no function to arrange and take away before the automatic vacuum-cleaning. For this reason, the market for the cleaning robot is not yet built up. So, we need a design method and technological algorithm of new automatic machine to solve the problem of mess-cleanup in house. It needs functions of agile automatic navigation, novel manipulation system for mess-cleanup. The automatic navigation system has to be controlled for the full scanning of living room, to recognize the absolute position and orientation of tile self, the precise tracking of the desired path, and to distinguish the mess object to clean-up from obstacle object to just avoid. The manipulate,, which is not needed in the vacuum-cleaning robot, must have the functions, how to distinguish big trash to clean from mess objects to arrange, how to grasp in according to the form of mess objects, how to move to the destination in according to mess objects and arrange them. We use the RFID system to solve the problems in this paper and propose the reading algorithm of RFID tags installed in indoor objects and environments. Then, it should be an intelligent system so that the mess cleaning task can be autonomously performed in a wide variety of situations and environments. It needs to also has the entertainment functions for the good communication between the human and UHMR. Finally, the good performance of the designed UHMR is confirmed through the results of the mess clean-up and arrangement.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2005년도 ICCAS
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• pp.1612-1616
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• 2005

In this paper, a Home Mess-Cleanup Robot(HMR), which has a practical function of the automatic mess-cleanup, is developed. The vacuum-cleaner had made the burden of house chore lighten but the operation labour of a vacuum-cleaner had been so severe. Recently, the cleaning robot was producted to perfectly solve the cleaning labour of a house but it also was not successful because it still had a problem of mess-cleaning, which was the clean-up of big trash and the arrangement of newspapers, clothes, etc. The cleaning robot is to just vacuum dust and small trash but has no function to arrange and take away before the automatic vacuum-cleaning. For this reason, the market for the cleaning robot is not yet built up. So, we need a design method and technological algorithm of new automatic machine to solve the problem of mess-cleanup in house. It needs functions of agile automatic navigation, novel manipulation system for mess-cleanup. The automatic navigation system has to be controlled for the full scanning of living room, to recognize the absolute position and orientation of the self, the precise tracking of the desired path, and to distinguish the mess object to clean-up from obstacle object to just avoid. The manipulator, which is not needed in the vacuum-cleaning robot, must have the functions, how to distinguish big trash to clean from mess objects to arrange, how to grasp in according to the form of mess objects, how to move to the destination in according to mess objects and arrange them. We use the RFID system to solve the problems in this paper and propose the reading algorithm of RFID tags installed in indoor objects and environments. Then, it should be an intelligent system so that the mess cleaning task can be autonomously performed in a wide variety of situations and environments. It needs to also has the entertainment functions for the good communication between the human and HMR. Finally, the good performance of the designed HMR is confirmed through the results of the mess clean-up and arrangement.

• 한국산업보건학회지
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• 제21권4호
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• pp.201-208
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• 2011

Objectives: According to the compliance of the asbestos-related regulation, every building has to be inspected for asbestos presence before its abatement work. This study was performed for identifying the types and contents of asbestos in building bulk samples. Materials and Methods: Bulk samples were collected during the asbestos inspection in 2010. We grouped the bulk samples into the regulated asbestos containing materials(RACM), presumed asbestos containing materials(PACM), and construction products. Additionally, the types of asbestos in all bulk samples were identified by polarization microscopy(PLM). Results: The RACMs were from building, house, pipe and facility. The RACMs were found mainly building (72.1%) and house (93.7%). The contents of chrysotile in building, house and facility were 66.9% (1-90%), 89.7% (2-90%) and 11.0% (2-90%), respectively. PACMs were surfacing material, thermal system insulation (TSI), and miscellaneous material. The miscellaneous materials that showed a high detection rate (79.2%) were ceiling, roofing and wall materials. Among them, the roofing materials had high chrysotile content(9.7%, 2-21%), followed by wall (8.7%, 2-21%) and ceiling (3.4%, 1-17%). In the construction products, asbestos was found mainly in slate (92.6%, 2-21%), including chrysotile. The slate had high asbestos content (9.7%, 2-21%), followed by cement flat board (8.7%, 2-19%) and textile (3.4%, 1-17%) Conclusions: Utilizing these results, it would be contributed to construct a useful ACM database and prevent from asbestos exposure to workers in the asbestos abatement and maintenance works.