• Title/Summary/Keyword: hot-plate test

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Desmutagenic Effect of Water Extract from Artemisia capillaris THUNB on the Mutagenicity of Benzo[a]pyrene (Benzo[a]pyrene의 변이원성에대한 인진쑥 물 추출물의 항돌연변이 효과)

  • 안병용
    • KSBB Journal
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    • v.15 no.4
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    • pp.331-336
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    • 2000
  • The antimutagenic activity of the extract of Artemisia capillaris THUNB on the mutagenicity induced by benzo(a)pyrene [B(a)P] in the presense of S9 mixture was studied using bacterial mutagenic assay system. Samples harvested in summer and autumn were extracted using ethanol and hot water. Among these extracts the water extract of summer sample had the strongest inhibitory effect against the mutagenenicity of B(a)P, The water extract of Artemisia capillaris THUNB was separated again into ethanol soluble and insoluble parts. The ethanol insoluble part(El) of water extract exhibited higher inhibition effects than the ethanol soluble part against the mutagenic activity of B(a)P. El showed dose-dependent activity on the mutagenicity of B(a)P in SOS Chromotest and Ames test. The 50% inbibition concentraction $(IC_{50}$ of El were $200{\mu}g/assay$ $600{\mu}g/plate$ and $800{\mu}b/plate$ in E. coil PQ37 S. typhimurium TA100 and TA98 respectively. El were showed desmutagenic effect but had no effect on the DNA repair system for B(a)P-induced mutagenesis. HPLC analysis showed that the formation of aflatoxin M1 by cytochrome P-450 1A1 known as playing an impotant role on B(a) P-induced mutagenicity was highly inhibited by El. Therefore we encluded that B(a)P-induced mutagencity can be reduced possible due to the interference of el with cytochrome P-450 1A1-dependent bioactivation.

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Quality Characteristics of Fresh Noodles With Hot-air-dried Perilla Leaf powder (열풍 건조 들깻잎 분말을 첨가한 생면의 품질특성)

  • Kim, Jung-Soo;Ahn, Jong-Sung;Ahn, Kwang-Yeol
    • Culinary science and hospitality research
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    • v.19 no.3
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    • pp.73-86
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    • 2013
  • This study produced hot-air-dried perilla leaf to add Korea's popular perilla leaf to fresh noodles to examine their value as food and get the best fresh noodle recipe by adding 0%, 3%. 6%, 9%, and 12% of perilla leaf to flour. The moisture content of the dough was highest at 34.41% for the control group with 0% perilla leaf, whereas the pH was lowest at 5.59 for the dough with 12% perilla leaf. As a result of WRC analysis of the dough, moisture absorption increased with greater perilla leaf content. The moisture content of fresh noodles was lowest for the control group with 0% perilla leaf, whereas the pH was highest for the control group. The L, a, and b values of the dough were highest for the group with 0% perilla leaf powder. Hardness and cohesiveness were lowest for the 0% group and springiness tended to decrease with increased amounts of hot air-dried perilla leaf. The number of microorganisms decreased significantly with greater perilla leaf content on the third and fourth days. As a result of the sensory test, the 6% dough showed the highest scores for all items including appearance, flavor, color, taste, and texture. Overall acceptability was also highest at 7.20 for the 6% dough and lowest at 3.27 for the 12% dough. Based on the above results, when producing fresh noodles by adding hot-air-dried perilla leaf powder, inhibition of microorganisms improved with greater perilla leaf content for longer storage, but the sensory properties were best when 6% perilla leaf content was added.

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Development of ELISA for detection of canine brucellosis (Canine brucellosis 검출을 위한 ELISA 진단법 확립)

  • Hur, Jin;Baek, Byeong-Kirl
    • Korean Journal of Veterinary Service
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    • v.34 no.2
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    • pp.159-166
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    • 2011
  • This study was carried out to develope enzyme-linked immunosorbent assay (ELISA) for detection of canine brucellosis in dogs experimentally inoculated with Brucella abortus 1119-3 and B. canis RM666. Groups A, B and C of dogs (each group consisting of three dogs) were orally inoculated with approximately $5{\times}10^9$ colony-forming units of B. abortus and B. canis, and with sterile pyrogen-free PBS, respectively. The animals were monitored at regular intervals upto the 12th week post inoculation (PI) by standard tube agglutination test (STAT), plate agglutination test (PAT), Rose Bengal test (RBT), 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and ELISA. The induced antibody titers in group A dogs were detected from the first week PI to the eighth week PI in STAT, PAT and RBT using the inactivated whole cells of B. abortus 1119-3 as antigens, while no sera in groups B and C dogs reacted with the antigens. In 2ME-RSAT using whole cells of B. canis M-strain as antigens, the induced antibody titers in group B dogs were observed at the second week PI and persisted for the 12th week PI, while sera of groups A and C dogs did not react with the whole cells. In ELISA using cytoplasmic fractions antigen of B. abortus 1119-3, the mean optical density of antibodies in groups A and B was detected from the first and second weeks PI, respectively, and persisted for 12th week PI, while sera of group C did not cross-react with the fractions antigen. However, in ELISA using the hot saline extracts of B. canis M- as an antigen, the induced antibody titers in only group B dogs were detected from second week PI and persisted for until the end of this study. These results indicate that the ELISA using B. abortus 1119-3 cytoplasmic fractions as antigens can be a good candidate for detection of brucellosis by B. abortus as well as B. canis in dogs.

Fabrication of Full-Scale Combustion Chamber of Liquid Rocket Engine for Ground Hot Firing Tests (실물형 고압 연소기의 연소시험 검증용 제작)

  • Kim Jonggyu;Seo Seonghyeon;Kim Seunghan;Han Yeoungmin;Ryu Chulsung;Seol Wooseok
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • v.y2005m4
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    • pp.305-308
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    • 2005
  • This paper presents a fabrication of a full-stale combustion chamber of a liquid rocket engine for a ground hot firing test. Engine drawings for manufacturing were prepared after conceptual and detail designs. The combustor is composed of a head and a chamber. SUS316L is used for materials of the head because of the good quality in low temperature. Inner materials of the ablative cooling chamber is silica/phenolic and outer case materials is the SUS316L. Materials of the regenerative cooling chamber are C18200 and SUS316L. After lathe, general milling and MCT machinings, components were finished by electrolytic polishing. A brazing method was applied for bonding the injectors and the injector plate, the regenerative cooling chamber because of structure configurations.

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Anti-inflammatory and anti-noceceptive action of the crude extracts of Costus specious on rodents

  • Alam, Ashraful;Subhan, Nusrat;Awal, Abdul;Alam, Shohidul;Akramudau, Kazi
    • Advances in Traditional Medicine
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    • v.8 no.3
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    • pp.243-251
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    • 2008
  • The effect of alcoholic extracts of Costus specious (Family: Zingiberaceae) was evaluated in experimental models of pain and inflammation. Oral administration of 100, 200 and 300 mg/kg of C. specious extracts were used for the above study. Crude extracts of C. specious (300 mg/kg dose) showed maximum time needed for the response against thermal stimuli ($7.242\;{\pm}\;0.532\;s$) which is comparable to diclofenac sodium ($8.471\;{\pm}\;0.25\;s$) in the hot plate test. The MPH (Maximum Possible Analgesia) has been found to be 14.285 for 300 mg/kg dose of the crude extract while the MPH for diclofenac was 15.857 after 60 min of administration in the hot tail-flick method. The crude extract at 300 and 200 mg/kg doses showed significant reduction in acetic acid induced writhings in mice with a maximum effect of 59.661% reduction at 300 mg/kg dose which is comparable to standard diclofenac sodium (73.4%). Alcoholic extract of C. specious showed significant inhibition in serotonin and egg albumin induced hind paw oedema in rats at 100, 200 and 300 mg/kg of the crude extracts respectively (Serotonin induced edema 44.22; 53.75; 58.51%; egg albumin induced edema - 41.317; 53.892; 59.880% inhibition after 4 h respectively). The antiinflammatory effects showed by the extract were comparable to that of standard indomethacin 5 mg/kg (Serotonin induced edema 77.56%; egg albumin induced edema 77.844% inhibition after 4 h). These results suggest that the extract possesses both the anti-inflammatory and analgesic activity on mice and rat model.

The Role of Slits and Swirl Vanes on the Turbulent Flow Fields in Cone Type Gas Burner (콘형 가스버너의 난류유동장에 대한 슬릿과 스월베인의 역할)

  • Kim, Jang-Kweon;Jeong, Kyu-Jo
    • Proceedings of the KSME Conference
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    • 2001.11b
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    • pp.341-346
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    • 2001
  • The gun-type gas burner adopted in this study is generally composed of some slits and swirl vanes. Therefore, this paper is studied to investigate the effect of slits and swirl vanes on the turbulent flow fields in the horizontal plane of gas swirl burner with a cone type baffle plate measured by using X-probe from hot-wire anemometer system. This experiment is carried out at flow rate $450\;{\ell}/min$, which is equivalent to the combustion air flow rate necessary for heat release 15,000 kcal/hr in gas furnace, in the test section of subsonic wind tunnel. When the burner has only swirl vanes, the axial mean velocity component shows the characteristic that spreads more remarkably toward radial direction than axial one, but when it has only slits, that is developed spreading more toward axial direction than radial one. Therefore, because the biggest speed is spurted in slits and it derive main flow toward axial direction encircling rotational flow that comes out from swirl vane that is situated on the inside of slits, both slits and swirl vanes composing of cone type gas burner act role that decreases the speed near slits and increases the flow speed in the central part of a burner. Moreover, because rotational flow by swirl vanes and fast jet flow by slits increase turbulent intensities effectively coexisting, the turbulent kinetic energy is distributed with a bigger size fairly near slits than burner models which have only slit or swirl vanes within X/R<0.6410.

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The Structure of Three-dimensional Turbulent Flow Fields of a Cone Type Gas Swirl Burner (콘형 가스 스월버너의 3차원 난류 유동장 구조)

  • Kim, J.K.;Jeong, K.J.
    • Journal of Power System Engineering
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    • v.5 no.2
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    • pp.22-29
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    • 2001
  • This paper represents axial mean velocity, turbulent kinetic energy and swirl number based on momentum flux measured in the X-Y plane and Y-Z plane respectively of a cone type gas swirl burner by using X-probe from the hot-wire anemometer system. This experiment is carried out at flow rates 350 and $450{\ell}/min$ respectively, which are equivalent to the combustion air flow rate necessary for heat release 15,000 kcal/hr in gas furnace, in the test section of a subsonic wind tunnel. Axial mean velocities and turbulent kinetic energies show that their maximum values exist centering around narrow slits situated radially on the edge of and in the forefront of a burner until $X/R{\fallingdotseq}1.5$, but they have a peculiar shape like a starfish diffusing and developing into inward and outward of a burner by means of the mixing between flows ejected from narrow slits, an inclination baffle plate and swirl vanes respectively according to downstream regions. Moreover, they show a relatively large value in the inner region of 0.5$S_m$ obtained by integration of velocity profiles shows a characteristic that has an inflection point composing of the maximum and minimum value until X/R<3, but shows close agreement with the geometric swirl number after a distance of X/R=3.

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An Experimental Study on the Three Dimensional Turbulent Flow Characteristics of Swirl Burner for Gas Furnace (가스난방기용 스월버너의 3차원 난류유동 특성에 관한 실험적 연구)

  • Kim, Jang-Gwon
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.25 no.2
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    • pp.225-234
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    • 2001
  • This paper represents the vector fields, three dimensional mean velocities, the turbulent intensities, the turbulent kinetic energy, and the Reynolds shear stresses in the X-Y plane of gas swirl burner with a cone type baffle plate measured by using X-probe from hot-wire anemometer system. This experiment is carried out at flow rates 350 and 450ℓ/min respectively, which are equivalent to the combustion air flow rate necessary for heat release 15,000 kcal/hr in gas furnace, in the test section of subsonic wind tunnel. The vector plot shows that the maximum axial mean velocity component exists in the narrow slits situated radially on the edge of gas swirl burner, for that reason, there is some entrainment phenomena of ambient air in the outer region of burner. Moreover, mean velocities in the initial region are largely distributed near the outer region of burner at Y/R≒0.97, but they diffuse and develop into the center flow region of burner according to the increase of axial distance. The turbulent intensities and the turbulent kinetic energy due to large inclination of mean velocity and swirl effect show that the maximum value in the initial region of burner is formed in the narrow slits situated radially on the edge of gas swirl burner and large values are mainly formed in the entire region of burner after X/R=2.4358, hence, the combustion reaction is anticipated to occur actively near this region. And the Reynolds shear stresses are also largely distributed from slite to vanes of gas swirl burner in the intial region, but their values largely disappear after X/R=3.2052.

Factor Analysis Affecting on Changes in Handysize Freight Index and Spot Trip Charterage (핸디사이즈 운임지수 및 스팟용선료 변화에 영향을 미치는 요인 분석)

  • Lee, Choong-Ho;Kim, Tae-Woo;Park, Keun-Sik
    • Journal of Korea Port Economic Association
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    • v.37 no.2
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    • pp.73-89
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    • 2021
  • The handysize bulk carriers are capable of transporting a variety of cargo that cannot be transported by mid-large size ship, and the spot chartering market is active, and it is a market that is independent of mid-large size market, and is more risky due to market conditions and charterage variability. In this study, Granger causality test, the Impulse Response Function(IRF) and Forecast Error Variance Decomposition(FEVD) were performed using monthly time series data. As a result of Granger causality test, coal price for coke making, Japan steel plate commodity price, hot rolled steel sheet price, fleet volume and bunker price have causality to Baltic Handysize Index(BHSI) and charterage. After confirming the appropriate lag and stability of the Vector Autoregressive model(VAR), IRF and FEVD were analyzed. As a result of IRF, the three variables of coal price for coke making, hot rolled steel sheet price and bunker price were found to have significant at both upper and lower limit of the confidence interval. Among them, the impulse of hot rolled steel sheet price was found to have the most significant effect. As a result of FEVD, the explanatory power that affects BHSI and charterage is the same in the order of hot rolled steel sheet price, coal price for coke making, bunker price, Japan steel plate price, and fleet volume. It was found that it gradually increased, affecting BHSI by 30% and charterage by 26%. In order to differentiate from previous studies and to find out the effect of short term lag, analysis was performed using monthly price data of major cargoes for Handysize bulk carriers, and meaningful results were derived that can predict monthly market conditions. This study can be helpful in predicting the short term market conditions for shipping companies that operate Handysize bulk carriers and concerned parties in the handysize chartering market.

A Defect Detection of Thin Welded Plate using an Ultrasonic Infrared Imaging (초음파 열화상 검사를 이용한 박판 용접시편의 결함 검출)

  • Cho, Jai-Wan;Chung, Chin-Man;Choi, Young-Soo;Jung, Seung-Ho;Jung, Hyun-Kyu
    • Journal of Institute of Control, Robotics and Systems
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    • v.13 no.11
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    • pp.1060-1066
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    • 2007
  • When a high-energy ultrasound propagates through a solid body that contains a crack or a delamination, the two faces of the defect do not ordinarily vibrate in unison, and dissipative phenomena such as friction, rubbing and clapping between the faces will convert some of the vibrational energy to heat. By combining this heating effect with infrared imaging, one can detect a subsurface defect in material efficiently. In this paper a detection of the welding defect of thin SUS 304 plates using the UIR (ultrasonic infrared imaging) technology is described. A low frequency (20kHz) ultrasonic transducer was used to infuse the welded thin SUS 304 plates with a short pulse of sound for 280ms. The ultrasonic source has a maximum power of 2kW. The surface temperature of the area under inspection is imaged by a thermal infrared camera that is coupled to a fast frame grabber in a computer. The hot spots, which are a small area around the defect tip and heated up highly, are observed. From the sequence of the thermosonic images, the location of defective or inhomogeneous regions in the welded thin SUS 304 plates can be detected easily.