• 한국발생생물학회지:발생과생식
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• 제6권2호
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• pp.83-88
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• 2002

월동기 90일 동안 transgenic coho salmon의 사료섭식량, 성장도 및 혈중 호르몬 수준을 파악하였다. 실험 종료시 실험구별 사료섭식량은 비교해 볼 때 tGH구가 Wild구보다 약4배 정도 높게 나타났지만, 사료효율의 경우 오히려 tGH구가 Wild구보다 1.1 배 낮은 것을 알 수 있었다. 한편 생존률은 tGH구가 Wild구보다 높게 나타났고, 성장은 tGH구가 길이에 있어 1.4배, 무게의 경우 약 3배 빠르게 나타났으며, 비만도 역시 tGH구가 Wild구보다 높게 나타났다. 실험구별 성장에 따른 호르몬 분석 결과, GH와 thyroid hormone의 농도는 tGH구가 Wild구에 비해 약 2배 정도 높은 값을 나타냈었다. 실험구별 형태적 특징을 관찰해 본 결과, tGH구들은 Wild구와 비교해 머리가 몸에 비해 상대적으로 크면서 꼬리지느러미의 가랑이 부분이 중심선으로 위, 아래가 확연히 분리되어 있지 않았다. 또한 몸통 부분은 길이에 비해 상대적으로 체고가 높게 나타났다.

• Journal of Nutrition and Health
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• 제39권8호
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• pp.748-755
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• 2006

The purpose of this study was to find out effects of treatment of ginsenoside Re, Rc and EGCG on mRNA expressions of leptin, hormone sensitive lipase (HSL) and resistin in 3T3-L1 adipocytes. The concentrations of EGCG were treated with $0.01{\times}10^{-7},\;0.1{\times}10^{-7},\;1{\times}10^{-7}\;and\;1{\times}10^{-6}\;or\;100{\mu}g/ml$ ginsenoside Re, Rc in culture cell for 13 days. mRNA expression of leptin wasn't expressed in preadipocyte but according to differentiation of adipocyte, the that of mRNA expression was decreased at gensenosids or EGCG treated cells compared with non treated adipocyte. Expression of HSL mRNA was increased in G-Re, G-Rc and EGCG treated cells compared with non treated cells. The resistin level was significantly decreased in adipocytes treated with G-Re, G-Rc and EGCG. These pattern was similar to leptin expression. These results support that treatment of gensenosides or EGCG in 3T3-L1 adipocyte resulted to affect of leptin and resistin as well as HSL mRNA levels, accordingly, levels of leptin and HSL will be acted by signalling body fat stores to the hypothalamus which in turn regulates food intake andenergy expenditure to maintain body weight homeostasis. And also regulation of resistin mRNA will prevent to diabetics attacked with obesity. In conclusion, we suggest that consumption of ginseng saponine or EGCG might prevent human diabetics or/and obesity.

• Journal of Preventive Medicine and Public Health
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• 제43권4호
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• pp.301-308
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• 2010

Objectives: In most DEHP exposure assessment studies, single spot urine sample was used. It could not compare the exposure level among studies. Therefore, we are going to represent the necessity of selection of proper sampling time of spot urine for assessing the environmental DEHP exposure, and the association urinary DEHP metabolites with steroid hormones. Methods: We collected urine and plasma from 25 men. The urine sampling times were at the end of the shift (post-shift) and the next morning before the beginning of the shift (pre-shift). Three metabolites of DEHP {mono(2-ethylhexyl) phthalate [MEHP], mono-(2-ethyl-5-hydroxyhexyl)phthalate [MEHHP], and mono(2-ethyl-5-oxohexyl)phthalate [MEOHP]} in urine were analyzed by HPLC/MS/MS. Plasma luteinzing hormone, follicle stimulating hormone, testosterone, and $17{\beta}$- estradiol were measured at pre-shift using a ELISA kit. A log-transformed creatinine-adjusted urinary MEHP, MEHHP, and MEOHP concentration were compared between the post- and pre-shift. The Pearson’s correlation was calculated to assess the relationships between log-transformed urinary MEHP concentrations in pre-shift urine and hormone levels. Results: The three urinary metabolite concentrations at post-shift were significantly higher than the concentrations in the pre-shift (p<0.0001). The plasma hormones were not significantly correlated with log-transformed creatinine - adjusted DEHP metabolites. Conclusions: To assess the environmental DEHP exposure, it is necessary to select the urine sampling time according to the study object. There were no correlation between the concentration of urinary DEHP metabolites and serum hormone levels.

• Food Science and Biotechnology
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• 제16권6호
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• pp.1046-1050
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• 2007

To investigate the growth promoting effects of an herbal medicine formulation (HM-10), Sprague Dawley (SD) male rats (3 weeks old) were divided into 3 groups (8 rats/group). The control group was given a daily oral administration of saline, and the treatment groups, HM-1 and HM-2, were given daily administrations of HM-10 (500 and 1,000 mg/kg BW, respectively). The cumulative tibial bone growth of the HM-1 and HM-2 groups (22.5 and 20.8 mm, respectively), and their cumulative femur bone growth (19.4 and 18.2 mm, respectively), were significantly different compared to the control group (7.5 mm of tibial growth and 7.7 mm of femur growth) (p<0.05). Lastly, the growth hormone levels of the HM-1 and HM-2 groups (1.70 and 1.79 ng/mL, respectively), as well as their insulin like growth factor 1 (IGF-1) levels (165.1 and 171.7 ng/mL, respectively) showed significant differences compared to the control (0.93 ng/mL of growth hormone and 125.6 ng/mL of IGF-1) (p<0.05).

• Journal of Oral Medicine and Pain
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• 제32권2호
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• pp.201-210
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• 2007

많은 질병들과 기존에 존재하는 신체적인 질병들은 스트레스로 인해 발병되거나 크고 작은 스트레스의 영향으로 악화된다. 스트레스의 연구에 호르몬을 사용하는 기본은 신체의 대부분 시스템이 스트레스를 받는 동안 변화를 보인다는 것과 이러한 변화에 호르몬이 스트레스와 확실하게 연관되어 있다는 것을 관찰하는 것이다. 개념적으로 스트레스에 있어서 교감신경계와 시상하부-뇌하수체-부신축의 활성화가 중심적인 역할을 한다는 것이 호르몬의 변화를 측정해야 할 충분한 근거를 제공한다. 에피네프린과 노르에피네프린과 같은 카테콜라민, 코티졸, 테스토스테론 그리고 성장호르몬 등은 스트레스에 예민한 반응을 보인다. 한편 스트레스 연구를 위한 타액표본은 혈액이나 요의 표본과는 달리 스트레스를 주지 않고도 표본을 얻을 수 있으며 신체적인 구속이나 윤리적인 문제 등을 염려하지 않고도 채취가 가능하다는 장점이 있다. 타액내의 호르몬의 수치는 혈액내의 호르몬 수치를 잘 반영하므로 스트레스와 관련된 구강안면통증의 연구에 있어서 타액내의 스트레스 호르몬에 활용도는 매우 높으리라고 사료된다.

• 한국발생생물학회지:발생과생식
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• 제25권4호
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• pp.225-234
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• 2021

The present study aimed to investigate the mechanism of cell surface receptor loss by two constitutively activating mutants (designated L469R, and D590Y) and two inactivating mutants (D417N and Y558F) of the luteinizing hormone receptor (LHR) in the Japanese eel Anguilla japonica, known to naturally occur in human LHR transmembrane domains. We investigated cell surface receptor loss using an enzyme-linked immunosorbent assay in HEK 293 cells. The expression level of wild-type eel LHR was considered to be 100%, and the expression levels of L469R and D417N were 97% and 101%, respectively, whereas the expression levels of D590Y and Y558F slightly increased to approximately 110% and 106%, respectively. The constitutively activating mutants L469R and D590Y exhibited a decrease in cell surface loss in a manner similar to that of wild-type eel LHR. The rates of loss of cell surface agonist-receptor complexes were observed to be very rapid (2.6-6.2 min) in both the wild-type eel LHR and activating mutants. However, cell surface receptor loss in the cells expressing inactivating mutants D417N and Y558F was slightly observed in the cells expressing inactivating mutants D417N and Y558F, despite treatment with a high concentration of agonist. These results provide important information on LHR function in fish and the regulation of mutations of highly conserved amino acids in glycoprotein hormone receptors.

• 농업과학연구
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• 제12권1호
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• pp.70-74
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• 1985

한국재래산양(韓國在來山羊)에 대하여 혈청(血淸) LH, FSH, prolactin, estradiol-$17{\beta}$ 및 progesterone의 농도(濃度)를 교배일(交配日)로부터 20일간격(日間隔)으로 140일(日)까지와 분만당일(分娩當日) 및 분만후(分娩後) 10일(日)과 20일(日)에 조사(調査)하였다. 혈청(血淸) LH의 농도(濃度)는 임신(姙娠) 60일(日)에 1.95mIU/ml로 최고치(最高値)였고, 임신(姙娠) 100일(日0까지는 높은 수준(水準)이었으나 그 후(後)로는 급격(急激)히 감소(減少)하여 분만시(分娩時)에는 0.02 mIU/ml를 나타냈다. Prolactin의 농도(濃度)는 임신(姙娠) 140일(日)부터 급격(急激)히 증가(增加)하여 분만시(分娩時)에는 29.75 ng/ml로 최고치(最高値)였으며, 분만후(分娩後)에는 다시 감소(減少)하였다. FSH는 모든 관찰시간(觀察時間)에서 분절하한치(分折下限値)인 1.25 mIU/ml 이하(以下)의 수준(水準)이었다. 혈청(血淸) estradiol-$17{\beta}$의 수준(水準)은 임신기간(姙娠期間)이 경과(經過)할수록 증가(增加)하였으며, 분만시(分娩時)에는 159.62 pg/ml로 최고치(最高値)였고, 분만후(分娩後)에는 월등(越等)히 감소(減少)하였다. 혈청(血淸) progesterone은 임신(姙娠) 120일(日)에 6.62 ng/ml로 최고치(最高値)였고, 그 후(後)로는 감소(減少)하여 분만시(分娩時)에는 1.25 ng/ml로 매우 낮은 수준(水準)이었다.

• Childhood Kidney Diseases
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• 제13권1호
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• pp.14-20
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• 2009

Growth retardation is a common consequenc of chronic kidney disease (CKD) in childhood. Many recent clinical and experimental data indicate that growth failure in CKD is mainly due to a relative GH insensitivity and functional IGF-I deficiency. Glucocorticoids also glucocorticoids interfere with the integrity of the somatotropic hormone axis at various levels. Over the past 10 years, recombinant growth hormone (rhGH) has been used to help short children with chronic kidney disease. A GH dosage of 0.35 mg/kg/week (28 IU/$m^2$/week) appears efficient and safe. Some clinical trial data show that final height will be within the normal target height range when GH treatment is continued for many years without remarkable adverse events.

• 대한수의학회지
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• 제39권2호
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• pp.276-286
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• 1999

In the present study, to understand how gonadotropin-releasing hormone (GnRH) affects ovarian functions in superovulated rats, we examined the effects of GnRH agonist on the ovulatory response, the morphological normality and nuclear maturation of ovulated oocytes, the ovarian weight, the ovarian histology, and the circulating steroid hormone ($17{\beta}$-estradiol, progesterone and testosterone) levels in immature rats pretreated with 30IU pregnant mare serum gonadotropin (PMSG) and supplemented with 10IU human chorionic gonadotropin(hCG). GnRH agonist was intravenously injected via jugular vein catheter every 20min for 4hrs in early follicular phase (from 6hr after PMSG) of superovulated rats. In addition, GnRH antagonist, Antide, was intravenously injected in combination with GnRH agonist to verify the effects of GnRH agonist on ovarian functions. All animals were sacrificed at 72hr after PMSG administration. The administration with GnRH agonist in early follicular phase of superovulated rats caused inhibition of ovulatory response, increased the proportion of abnormal appearing oocytes(especially, in the rats of the group treated with 500ng GnRH agonist), decreased ovarian weight and promote follicular atresia, compared to those from the rats of control regimen that were not treated with GnRH agonist. In addition, the treatment with GnRH agonist in the superovulated rat distinctly decreased serum steroid hormone ($17{\beta}$-estradiol, progesterone and testosterone) levels in preovulatory phase. On the other hand, the inhibitory effects of GnRH agonist treatment in superovulation-pretreated rats on ovarian functions were totally reversed by the combination with GnRH antagonist, Antide. The nuclear maturation of oocytes recovered from the oviducts in immature rats treated with GnRH agonist and/or GnRH antagonist was characterized by prematurity and asynchronization in early follicular phase, which was similar to control group. The overall results of this study indicate that GnRH agonist disturbs directly ovarian function in early follicular phase of superovulated immature rats in terms of ovulatory response and morphological normality of ovulated oocytes. This concept has been further evidenced by the findings of a great decrease in ovarian weight, a marked increase in follicular and a distinct decrease circulating steroid hormone ($17{\beta}$-estradiol, progesterone and testosterone) levels in GnRH agonist treatment regimen in early follicular phase.

• The Korean Journal of Pain
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• 제12권1호
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• pp.27-35
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• 1999

Background: Fibromyalgia is a common syndrome of musculoskeletal pain and fatigue. Lacking distinctive histological or laboratory abnormality in diagnosis, it has often been considered a form of "psychogenic rheumatism". Fibromyalgia causes much distress to the affected patients and often frustrates physicians, who are unable to start rational therapy on any logical disease pathology. Methods: Growth hormone is essential for muscular homeostasis. In the present study, the notion that the stage-4 sleep anomaly typically seen in the fibromyalgia syndrome may disrupt growth hormone secretion was tested. Because growth hormone has a very short half-life, serum levels of somatomedin C were measured; somatomedin C is the major mediator of growth hormone's anabolic actions and is a prerequisite for normal muscle homeostasis. Serum levels of somatomedin C using acid-extraction procedure and two-site immunoradiome-tric assay (IRMA) and number of tender points were measured in 27 female patients with fibromyalgia from 40 to 60 years old and 27 healthy controls. Results: There were no differences in the concentration of somatomedin C between fibromyalgia patients and controls ($mean{\pm}SD$: $178.3{\pm}75.5$ ng/ml versus $166.3{\pm}76.6$ ng/ml; p=0.55). And there were no correlations between number of tender point and serum somatomedin C level by linear regression analysis. Conclusions: These findings did not support that there is a distinctive disruption of the growth hormone-somatomedin C neuroendocrine axis in a fibromyalgia syndrome. But we can not discard the hypothesis that disturbed sleep predispose to muscle pain.