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Thinning and drilling laser-assisted hatching in thawed embryo transfer: A randomized controlled trial

  • Le, Minh Tam;Nguyen, Thi Tam An;Nguyen, Thi Thai Thanh;Nguyen, Van Trung;Le, Dinh Duong;Nguyen, Vu Quoc Huy;Cao, Ngoc Thanh;Aints, Alar;Salumets, Andres
    • Clinical and Experimental Reproductive Medicine
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    • v.45 no.3
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    • pp.129-134
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    • 2018
  • Objective: In frozen and thawed embryos, the zona pellucida (ZP) can be damaged due to hardening. Laser-assisted hatching (LAH) of embryos can increase the pregnancy rate. This study compared thinning and drilling of the ZP before frozen embryo transfer (FET). Methods: Patients were randomly allocated into two groups for LAH using thinning or drilling on day 2 after thawing. Twenty-five percent of the ZP circumference and 50% of the ZP thickness was removed in the thinning group, and a hole $40{\mu}m$ in diameter was made in the drilling group. Results: A total of 171 in vitro fertilization/intracytoplasmic sperm injection FET cycles, including 85 cycles with drilling LAH and 86 cycles with thinning LAH, were carried out. The thinning group had a similar ${\beta}$-human chorionic gonadotropin-positive rate (38.4% vs. 29.4%), implantation rate (16.5% vs. 14.4%), clinical pregnancy rate (36.0% vs. 25.9%), miscarriage rate (5.8% vs. 2.4%), ongoing pregnancy rate (30.2% vs. 23.5%), and multiple pregnancy rate (7.0% vs. 10.6%) to the drilling LAH group. There were no significant differences in pregnancy outcomes between subgroups defined based on age (older or younger than 35 years) or ZP thickness (greater or less than $17{\mu}m$) according to the LAH method. Conclusion: The present study demonstrated that partial ZP thinning or drilling resulted in similar outcomes in implantation and pregnancy rates using thawed embryos, irrespective of women's age or ZP thickness.

Results of Cold Flow Test and Design of Injectors for Oxidizer-rich Preburner (산화제 과잉 예연소기용 분사기 설계 및 수류 시험 결과)

  • So, YoonSeok;Woo, SeongPil;Lee, Kwang-Jin;Yu, ByungIl;Kim, Jinhyung;Cho, Hwangrae;Bang, Jeongsuk;Han, YeongMin
    • Journal of the Korean Society of Propulsion Engineers
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    • v.22 no.1
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    • pp.52-57
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    • 2018
  • This paper presents the design and cold flow test results of oxidizer-rich preburner injectors for a 9 tonf-class staged combustion engine cycle. Three types of coaxial swirl injectors were designed, and 12 injectors were designed for each type. The diameters of the fuel tangential holes are identical. The diameters of the oxidizer tangential holes were varied to investigate the influence of combustion in the oxidizer-rich preburner according to the momentum ratio of the gas oxidizer generated from combustion in the injector chamber and liquid oxidizer through the cooling channel. It will be verified through a powerpack and combustion test using an oxidizer-rich preburner. In the cold flow test, the fuel flow rate and oxidizer tangential hole flow rate reached the target value based on the designed differential pressure.

Soil Mineral Nutrients and Microbes Are Responsible for Large Patch Disease Caused by Rhizoctonia solani AG2-2 in Zoysiagrass Turf (골프장 한국잔디의 Rhizoctonia solani AG2-2에 의한 Large Patch 발생 토양에서 근권 미생물과 무기영양 평가)

  • Chang, Tae-Hyun;Ru, Yeon-Ju;Lee, Yong-Se
    • Asian Journal of Turfgrass Science
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    • v.21 no.2
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    • pp.113-126
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    • 2007
  • Mineral nutrients and population dynamics of soil microbes in the root zones of zoysiagrass infected by Rhizoctonia solani AG2-2 and that of healthy plants were sampled from ten golf courses using a cup cutter(diameter $10\;cm\;{\times}\;8\;cm$ deep). Analysis of variance(ANOVA) showed significant differences in content of $NO_3$-N(P = 0.05), $NH_4$-N(P = 0.1), and K(P = 0.1) between infected and healthy samples. The content of $NO_3$-N in the soils of large patch was 9.49 mg/kg and that in soil of healthγ plants was 7.02 mg/kg. However, the content of $NH_4$-N in the soil of large patch was 12.02 mg/kg whereas 14.40 mg/kg for the soil under the healthy plants. The content of K in the soil of large patch was lower than that of soil of healthy plants. There was few numbers of Pseudomonas colonies In the soils of large patch compared to that of healthy plants. These results indicated that the content of $NO_3$-N, NH4-N, and K and the microbial population dynamics in root zones correlated to occurrence of large patch.

Single mode yield analysis of index-coupled DFB lasers above threshold for various facet reflectivity combinations (Index-coupled DFB 레이저의 여러 가지 양 단면 반사율 조합에 따른 문턱 전류 이상에서 단일 모드 수율 해석)

  • 김상택;전재두;김부균
    • Korean Journal of Optics and Photonics
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    • v.14 no.3
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    • pp.298-305
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    • 2003
  • We have calculated the single mode yield of index-coupled (IC) DFB lasers above threshold for several kL, and facet reflectivity combinations, and investigated the correlation between those results and the single mode yield as a function of f number at the threshold. As a result, there is little correlation between the single mode yield above threshold and the single mode yield as a function of f number at the threshold. The single mode yields above threshold for kL of 0.8 and 1.25 is larger than those for kL, of 2 and 3 due to the spatial hole burning effect. Also, we have investigated the effect of the reflectivity of the AR facet on the single mode yield for AR-HR and AR-CL combinations. For AR-HR combinations, the single mode yield increases as the reflectivity of the AR facet decreases. However, for AR-CL combinations, the reflectivity of the AR facet for the largest single mode yield exists. In the single mode yield calculations for IC DFB lasers in this paper, the single mode yield for kL of 0.8 with AR(1%)-HR combination is largest above threshold.

Microfluidic Assisted Synthesis of Ag-ZnO Nanocomposites for Enhanced Photocatalytic Activity (광촉매 성능 강화를 위한 미세유체공정 기반 Ag-ZnO 나노복합체 합성)

  • Ko, Jae-Rak;Jun, Ho Young;Choi, Chang-Ho
    • Clean Technology
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    • v.27 no.4
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    • pp.291-296
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    • 2021
  • Recently, there has been increasing demand for advancing photocatalytic techniques that are capable of the efficient removal of organic pollutants in water. TiO2, a representative photocatalytic material, has been commonly used as an effective photocatalyst, but it is rather expensive and an alternative is required that will fulfill the requirements of both high performing photocatalytic activities and cost-effectiveness. In this work, ZnO, which is more cost effective than TiO2, was synthesized by using a microreactor-assisted nanomaterials (MAN) process. The process enabled a continuous production of ZnO nanoparticles (NPs) with a flower-like structure with high uniformity. In order to resolve the limited light absorption of ZnO arising from its large band gap, Ag NPs were uniformly decorated on the flower-like ZnO surface by using the MAN process. The plasmonic effect of Ag NPs led to a broadening of the absorption range toward visible wavelengths. Ag NPs also helped inhibit the electron-hole recombination by drawing electrons generated from the light absorption of the flower-like ZnO NPs. As a result, the Ag-ZnO nanocomposites showed improved photocatalytic activities compared with the flower-like ZnO NPs. The photocatalytic activities were evaluated through the degradation of methylene blue (MB) solution. Scanning electron microscopy (SEM), x-ray diffraction (XRD), and energy-dispersive x-ray spectroscopy (EDS) confirmed the successful synthesis of Ag-ZnO nanocomposites with high uniformity. Ag-ZnO nanocomposites synthesized via the MAN process offer the potential for cost-effective and scalable production of next-generation photocatalytic materials.

Some Biological Characteristics of Plum Sawfly Monocellicampa pruni (Hymenoptera: Tenthredinidae) (자두수염잎벌(Monocellicampa pruni)의 생물학적 특성)

  • Nguyen, Nam Hai;Ki, Woong;Im, Min-Hyeok;Hong, Ki-Jeong
    • Korean journal of applied entomology
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    • v.60 no.4
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    • pp.479-487
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    • 2021
  • This study investigated the life history of the plum sawfly Monocellicampa pruni Wei, 1998 through field observations and laboratory experiments. M. pruni is a univoltine, complete metamorphosis species with five instars at the larval stage. Adults emerge from the soil around mid-March when Japanese plum trees are in their flowering period. The adults deposit one (rarely two) egg(s) under the epidermis layer of the calyx. After hatching, the larva soon burrows into the fruitlet, consumes the endocarp and remains inside the fruit within its four-time moulting transitions before reaching full growth. At maturity (in May), the larva leaves the infested fruitlet through an exit hole and burrows into the soil at a depth of 2 - 11 cm for spinning its cocoon and hibernates until the next spring as the larval form. Under laboratory conditions (T = 20℃; RH = 40 - 60%), the male lived for a slightly shorter period than the female, 6.03 ± 0.40 and 7.55 ± 0.45 days, respectively. A female could produce 30.29 ± 4.50 eggs in her lifespan. In the field, the duration of the egg was approximately 10 - 11 days and that of the larva was approximately 31 - 34 days.

Effect of Canopy Covering on Thermal Insulation and Freezing Tolerence of 'Shiranui' Hybrid Mandarin Cultivated in Field During Winter Season (수관 피복이 노지재배 '부지화' 감귤나무의 보온과 내한성에 미치는 영향)

  • Joa, Jae-Ho;Kang, Seok-Beom;Moon, Young-Eel;Lee, Hae-Jin
    • Journal of Bio-Environment Control
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    • v.30 no.4
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    • pp.441-447
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    • 2021
  • It were investigated changes in internal and external temperature, relative humidity using tyvek, weedstop, and 35% shading net as covering material to reduce the freezing damage of 'Shiranui' hybrid mandarin grown in open field. It were also evaluated canopy covering effect and LT50 of leaves by covering material when it was cold at -2℃. In tyvek, temperature difference between inside and outside was low at the height of 1.5m and was high at 0.4m. The relative humidity differed greatly between day and night, and was high at 6-8 a.m. At -2℃, Sum of temperature at the height of 1.5m of canopy for 24-hour after covering were at 3.4℃ higher in tyvek than in control. The LT50 of leaves was at 1.51℃ in tyvek, 1.33℃ in withstop, and 1.61℃ in 35% shading net lower than in control. Considering thermal insulation and relative humidity in canopy, tyvek is expected to reduce low temperature damage when covering after making a fine hole for ventilation.

Numerical Analysis on Effect of Stemming Condition in Mine Ventilation Shaft Blasting (광산 통기수갱발파에서 전색조건이 발파효율에 미치는 영향에 관한 수치해석적 연구)

  • Kim, Jun-ha;Kim, Jung-gyu;Jung, Seung-won;Ko, Young-hun;Baluch, Khaqan;Kim, Jong-gwan
    • Explosives and Blasting
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    • v.39 no.3
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    • pp.15-23
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    • 2021
  • Ventilation shafts are pathways in mines and tunnels for the removal of dust or smoke during underground space construction and operation. In mines, blasting with long blast holes is preferred for the excavation of a ventilation shaft in the 10~20m long crown pillar section. In this case, the bottom part of the blast hole is completely drilled in order to determine the drilling error, and this causes a problem of lowering the explosive charge and blasting efficiency. It is possible to solve the problem of explosive loading and to increase the blast efficiency by covering the curb of the blasthole by using stemming material. In this study, simulations for the blasting of a ventilation shaft were performed with various stemming lengths and the blasthole diameters(45, 76mm) using AUTODYN 2D SPH(Smooth particle hydrodynamics) analysis technique. Also the optimal bottom stemming column was derived by checking the size of the boulder and burden line according to blasting. Analysis result, blasting efficiency is lessened in case of stemming length less than 30cm and the optimal length of the stemming material should be 30cm or higher to achieve high efficiency of blasting.

Smoke Control Experiment of a Very Deep Underground Station Where Platform Screens Doors are Installed - Analysis on Smoke Control Performance by Fans equipped in Tunnel (스크린도어가 설치된 대심도 지하역사의 제연 실험 - 터널 송풍기에 의한 제연의 효과 분석)

  • Park, Won-Hee;Kim, Chang-Yong;Cho, Youngmin
    • Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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    • v.9 no.9
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    • pp.721-736
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    • 2019
  • In this paper, the behavior of the fire smoke due to the operation of the ventilation systems when the fire occurred in the underground station (6 basement floors) and the tunnel at the great depth was measured. Fire smoke was generated by using a smoke generator which realized heat buoyancy effect by using hot air blower. The two locations of the fire were selected on the platform and on the platform of the tunnel located outside the screen door. A ventilation mode is generally used in which smoke is exhausted through a vent hole provided in a platform when a platform fire occurs. The tests were performed by operating the exhaust through the ventilation holes of the tunnel part located at both ends of the platform. The smoke density and the wind speed/velocity were measured at various positions, and the videos were taken to analyze the movement and smoke of the smoke. In both cases for fire inside the platform and in the railway tunnel, due to the ventilation mode operation of the fan for the platform and the exhaust of the fans in the tunnel smoke were well exhausted and the smoke propagation to the area near the smoke zone was suppressed. The smoke-control mode, which is applied to both fans for the platform and fans for in the tunnel at both ends of the platform, can provide a safer evacuation environment to the passengers from the fire smoke when the platform fire or fire train stops.

A New Japanese Apricot (Prunus mume) Cultivar, "Okboseok" for High Yield (다수확 매실 신품종 "옥보석")

  • Jeong, Sang-Bouk;Kim, Yoon-Kyeong;Kang, Sam-Seok;Cho, Kwang-Sik;Choi, Jang-jeon
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.534-538
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    • 2009
  • Japanese apricot (Prunus mume) "Okboseok" was released from a cross between "Nanko" and "Yoseibai" in 1993 at the Pear Research Station of National Institute of Horticultural and Herbal Science, Rural Development Administration. It was preliminarily selected in 2001 and named in 2006. It produces high yield with a flesh about average 88.3% of fruit, although the fruit size is small (approximately 14.0g). Horticultural and physiological harvest time of this cultivar is mid and late June respectively. It has single flowers with pink petal color and its flowering time is about 4 days later than that of "Nanko". Its fruit is tolerant to bacterial shot hole (Xanthomonas arboricola pv. Pruni) and pear scab (Venturia nashicola). "Okboseok" has abundant pollen grains (about 96 mg/100 flowers), thus it could be a pollinizer. To obtain stable yields, however, its compatibility with major cultivars is needed to be confirmed as pollinizer cultivar. "Okboseok" is recommended for being preserved in sugar and liquor.