• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2003년도 추계학술대회초록집
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• pp.7-7
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• 2003

Histological examination of biopsy or postmortem lung tissue from patients with asthma usually reveals thickened airway walls. This change is called airway remodeling, which is characterized by airway eosinophilia, hyperplasia of goblet cells and smooth muscle, and subepithelial fibrosis [1,2]. In this study, we investigated the time-course functional, morphological, biochemical changes of remodeling in a ovalbumin (OVA)-induced murine chronic asthma model. (omitted)

• 한국발생생물학회지:발생과생식
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• 제17권4호
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• pp.427-434
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• 2013

The histological changes in the Ussurian bullhead, Leiocassis ussuriensis, and the Korean bullhead, Pseudobagrus fulvidraco, were observed during the early period of growth. The retinas size of both species increased in the 9 days post-hatching (DPH) (p<0.05). In the just-hatched Ussurian bullhead, the retina already consisted of six layers: the epithelial layer, ganglion cell layer, inner nuclear layer, inner plexiform layer, outer limiting membrane layer, and rod and cone layer. The Korean bullhead had the same components. At 50 DPH, the thickness of the retina was $538.0{\pm}7.19{\mu}m$ in the Ussurian bullhead and $558.9{\pm}9.44{\mu}m$ in the Korean bullhead. The relative thickness of each layer of the retina did not differ significantly in the two species. Although the growth of the Korean bullhead's retina was faster, the relative thickness of each layer in the retina did not change during early development. After hatching, some parts of the tissue gradually became denser. Immediately after hatching, the kidney and midgut epithelium of the Ussurian bullhead and Korean bullhead were already formed and grew gradually thereafter. From 0 DPH to 30 DPH, the nuclear height in the midgut epithelium did not differ significantly between the two species, but at 50 DPH, it was $11.4{\pm}2.45{\mu}m$ in the Korean bullhead and $9.9{\pm}2.13{\mu}m$ in the Ussurian bullhead. During the experimental period, the major axes, minor axes, surface areas, and volumes of the proximal tubule cells in the kidney did not differ significantly between the two species. Thus, the early histological development of the Ussurian bullhead is similar to that of the Korean bullhead.

• 한국패류학회지
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• 제27권4호
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• pp.283-289
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• 2011

염분변화에 따른 북방전복의 생리적 반응을 조사하기 위하여 고염분 및 저염분의 농도에 따라 생존율, 삼투질농도, 대사율 및 조직학적 반응을 분석하였다. 염분변화에 따른 북방전복의 생존율은 37 psu에서는 100%였다. 반면 19.8 psu에서 노출 6일째, 12.8 psu 이하에서는 노출 24시간 이내 모두 사망하여 북방전복의 7-days $LS_{50}$은 24.9 psu (20.1-28.2 psu)를 나타내었다. 염분변화에 따른 호흡률은 대조구에 비해 감소하였으며, 반면 암모니아질소 배설률은 증가하였다. 삼투질농도는 염분 26.8 psu이상에서는 1시간 이내 순치하였다. 북방전복 발근육의 조직학적 반응은 염분감소에 따라 혈림프동의 확장, 상피세포의 괴사 및 식세포 증가 등의 현상이 관찰되었다.

• Environmental Analysis Health and Toxicology
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• 제21권2호
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• pp.115-126
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• 2006

This study was conducted to find out survival rate, activity, growth and change of the organ structure of bivalves exposed to heavy metal. The results of the study confirmed that zinc (Zn) induces reduction of survival rate and activity, abnormality of organ structure of the equilateral venus, Gomphina veneriformis. Experimental groups were composed of one control condition and three Zn exposure conditions ($0.64mg\;Zn{\iota}^{1},\;1.07mg\;Zn{\iota}^{-1},\;1.79mg\;Zn{\iota}^{-1}$). As the concentration of zinc increased the accumulation of lipofucin increased in the digestive gland. Survival rate was the lowest in the lowest Zn exposure group at $0.64mg;Zn{\iota}^{-1}$. Growth was not significantly different between the control and exposure group. Activity. with the exception of the lowest Zn exposure group at $0.64mg\;Zn{\iota}^{-1}$, was similar between the control and exposure group. Histological analysis of organ system illustrated expansion of hemolymph sinus, loss of striated border of inner epidermis, increase in the number of mucous cell in the mantle. Also, histological degenerations as epithelial necrosis and hyperplasia of mucous cells are recognized in the gill and foot.

• 환경생물
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• 제22권1호
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• pp.133-140
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• 2004

유기주석화합물이 넙치의 기관계 구조에 미치는 독성을 알아보기 위하여 넙치를 대조구와 TBTO를 첨가한 6개 농도구(0.17, 0.36, 0.60, 3.20, 6.30, 12.50 ${\mu}g \;L^{-1}$)에서 42일 동안 노출 실험을 하였다. 조직상의 변화 정도는 노출 시간과 TBTO농도의 증가에 따라 심하게 나타났다. 저농도의 TBTO 노출구에서 관찰된 초기 조직학적 변화로는 아가미에서는 점액세포와 염세포의 활성, 새판 모세혈관의 충혈, 상피층의 비후, 간췌장에서는 간세포의 활성화와 췌장 효소원과립의 감소, 담관의 변성이 확인되었으며, 신장에서는 모세혈관의 충혈, 호산성세포와 혹색 대식세포군의 출현이 관찰되었다. 고농도의 TBTO 노출구에서 관찰된 조직학적 변화로는 아가미에서는 새판 상피층의 박리와 새판의 붕괴, 간췌장에서는 간세포의 핵응축, 세포질의 혼탁 및 과립변성, 신장에서는 조혈세포의 핵응축, 사구체와 세뇨관의 붕괴 등 정상적인 기능 수행이 어려울 것으로 판단되는 조직상들이 관찰되었다. 이와 같은 결과는 TBTO는 다른 수중오염원과 마찬가지로 어류 기관계의 조직병리학적 변화를 유도한다는 것을 나타낸다.

• Asian-Australasian Journal of Animal Sciences
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• 제21권6호
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• pp.824-830
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• 2008

Sodium hydroxide (SH) or ammonium bicarbonate (AB) were applied to rice straw to investigate the effects on histological change of stem tissue or cell wall before and after in sacco degradation using a scanning electron microscope (SEM) and a transmission electron microscope (TEM). The SEM revealed that, the parenchyma and vascular bundles were distorted by treatment with SH at 30 or 45 g/kg straw dry matter. Faultage between phloem of large vascular bundles and parenchyma occurred with further increasing SH to 60 or 75 g/kg. The cell wall in these stem tissues was crimped when observed by TEM. However, only parenchyma and large vascular tissues were slightly distorted in AB-treated stem. For untreated and AB-treated stems, the initiation of observable ruminal degradation of cell wall was prolonged from 12 h for inner parenchyma to 24 h for sclerenchyma and to 48 h for phloem of small vascular bundles, while the outer epidermis was intact even at 72 h. For SH-treated stem, however, the cell wall from all of the investigated tissues, epidermis, small vascular bundles, sclerenchyma, and parenchyma started to be degraded at 12 h incubation. These results indicate that SH treatment contracts rice straw stem leading to an improvement in rumen degradation, and that the degradation of SH-treated stem is bilateral from inner and outer surface simultaneously.

• 한국발생생물학회지:발생과생식
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• 제4권1호
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• pp.13-17
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• 2000

FSH는 미성숙 설치류의 난포성장을 촉진하며, 강소형성 난포의 퇴화비율을 감소시킨다. 본 연구는 미성숙 생쥐에 난포성숙호르몬을 투여한 후 유발되는 난포의 조직학적인 변화를 규명하기 위해 시행되었다. 3주령의 ICR생쥐에 10 i.u.의 재조합 난포자극호르몬을 복강주사한 후 1일, 2일, 3일에 좌측 난소의 조직학적 변화를 관찰하였다. 강소형성전 난포의경우 FSH처리 후 시간에 따라 퇴화난포의 비율이 증가하였으나 강소형성 난포의 경우에는 유의한 변화를 보이지 않았다. 퇴화되는 양상은 난포 내 세포자연사하는 과립세포의 증가, 대식세포 및 다형다핵백혈구의 증가 등이 관찰되었다. 이상의 결과로 보아, 과량의 FSH처리 후에 유발되는 난포의 퇴화는 과립세포의 세포자연사뿐 아니라 급성 염증반응을 수반하는 것으로 생각된다.

• 대한의생명과학회지
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• 제14권4호
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• pp.211-218
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• 2008

The purpose of this study was to investigate the effects of dietary Chungkookjang (Korean fermented soybean) powder on blood glucose level, lipid profiles, antioxidant enzymes activities and histological changes in kidney of streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley rats of three groups including nondiabetic group fed normal diet (NC), diabetic group fed normal diet (DC) and diabetic group fed Chungkookjang diet (DCH; 100 g/kg diet) were reared for 8 weeks. The serum glucose, triglycelide and total lipid levels in the DCH group were significantly lower (P<0.05) than the DC group. The renal xanthine oxidase, catalase and glutathione S-transferase activities in the DC group were significantly higher than the NC group. The xanthine oxidase, superoxide dismutase and glutathione S-transferase activities in the DCH group were significantly lower than the DC group (P<0.05). Tubular epithelial change, such as Armanni-Ebstein cells, was significantly reduced in the DCH group compared to the DC group. In conclusion, these results indicated that Chungkookjang supplement seems to be beneficial to correct the hyperglycemia and hyperlipidemia as well as to protect kidney against diabetic changes.

• 한국환경보건학회지
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• 제35권5호
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• pp.417-425
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• 2009

The purpose of this study was to investigate the whitening effect of Angelicae dahuricae Radix water extract (ADWE) by its application on artificial tanning spots which were induced by 1,500 $mJ/cm^2$ of UVB radiation on the backs of brown guinea pigs weighing approximately 450~500 g. Thirty ${\mu}l$ of ADWE, at each application, were applied twice a day, 5 days a week, for 8 weeks to the guinea pigs. The artificial tanning spots were divided into 3 groups which were vehicle control group [propylene glycol: ethanol: water (5:3:2)], positive control group (2% hydroquinone) and experimental group (2% ADWE). The visible whitening effect and changes in melanin index were evaluated once a week. On completion of the experiment, the animals were sacrificed under anesthetization, and the artificial tanning spots were excised by biopsy punch and stained with Hematoxylin and Eosin (H&E) to observe histological change and also stained with Fontana-Masson's silver (F-M) and S-100 to observe melanin pigmentation and melanocytes. In the gross observation, the experimental group showed higher pigmentation than the positive control group but lower pigmentation than the vehicle control group. Histological observation confirmed that ADWE had a positive whitening effect by showing a lower distribution of melanin and melanocytes in the epidermis of experimental group than in the vehicle control group.

• 한국가축번식학회지
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• 제9권2호
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• pp.113-123
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• 1985

1. The weights of ovary and uternus in the all treated groups were lighter than those in control group showing significantly differences from 4 weeks after treatment. The significant was not recognized between PTU and Thx. groups, and Thyro. and control groups. 2. In the histological changes of ovary, follicles were disa, pp.ared and alignment of membrana granulosa disnitergration in cell change were serious in Thx. and PTU groups and slight in Thyro. group. 3. In the histological changes of uterus, endometrial epithelium and lamina propria were atrophied from 3 weeks after treatment in Thx. group, from 4 weeks in PTU groups and from 5 weeks in Thyro. group. Muscular layers were atrophied with time elapse in the all treated groups. 4. The changes of the concentrations of serum progesterone at all observation times in Thx. and PTU groups were significantly decreased in comparison with those in control group. While those in Thyro. group were significantly increased in comparison with those in control group. 5. The concentration of serum estradiol-17$\beta$ at all observation times in all experimental groups were below 27.2pg/ml. Therefore, we did not detect any changes of the concentrations.