• Title/Summary/Keyword: histochemical localization

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A study on pearl millet (Pennisetum glaucum L.) plant Biochemical and histochemical changes inoculated with indigenous AM fungi under Barren soil

  • Pal, Ajay;Pandey, Sonali
    • Journal of Plant Biotechnology
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    • v.44 no.2
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    • pp.203-206
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    • 2017
  • The soil organisms that develop beneficial Symbiotic relationships with plants roots and contribute to plant growth are mycorrhizal (AM) fungi. Arbuscular mycorrhizal inoculations change the growth and biochemical composition of the host plant and soil. Mycorrhizal root systems do augment the absorbing area of roots from 10 to 100 times thereby greatly improving the ability of the plants to utilize the soil resources. A pot experiment was conducted during the kharif seasons at Jaipur, Rajasthan, to find out the effects of three different indigenous AM fungi i.e. Glomus mosseae, Glomus fasciculatum and Gigaspora decipiens either single and in combination inoculation on biochemical and histochemical changes of Pearl millet (Pennisetum glaucum L.) grown under barren soil conditions. The AM fungus has shown to improve the tolerance of plant to drought stress. Experimental results showed that AM fungi treated plants improved their plants growths, biochemical and histochemical changes as compared to non-mycorrhizal treatments. The AM fungi inoculated plant was found to be attaining maximum plant biochemical and histochemical substances in Glomus mosseae (alone) and also Glomus mosseae + Glomus fasciculatum treatments.

Purification and Characterization of Glutathione Peroxidase Isolated from Rat Erythrocyte and Histochemical Study of its Localization in Liver of White Rat (흰쥐 적혈구에 있는 Glutathione Peroxidase의 순화 및 성질과 간에서의 용작부위에 대한 조직화학적 연구)

  • 최임순;최춘근
    • The Korean Journal of Zoology
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    • v.29 no.2
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    • pp.141-158
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    • 1986
  • A glutathione peroxidase from white rat (Wistar strain)erythrocytes was partially purified and characterized. In addition, localization of this enzyme in the liver was studied by histochemical method. A glutathione peroxidase was purified approximately 33.5-folds by ammonium sulfate precipitation, Sephadex filtration column and DEAE-Sephadex column chromatography. The optimum temperature of the crude glutathione peroxidase was $40^\\circC$, and the optimum pH was 7.5. This crude glutathione peroxidase was most stable at $30^\\circC$ and the values of Km and Vmax were calculated to be 8.5mM and 15.6 $\\mu$moles/min for glutathione, and 40 $\\mu$M and 10.5 $\\mu$moles/min for hydrogen peroxide, respectively. The molecular weight of this enzyme was estimated by Sephadex G-200 gel filtration to be approximately 90, 000. By electron microscopic examination, histochemical reaction products were microbodies that were prominent in the peripheral parts of the lobule. The reaction products exhibited round shapes, the diameter of which varied $0.2\\sim0.7 \\muM$ and their boundary membranes were not distint.

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Tissue Specific Expression of Wound-Inducible RCaM-2 Promoter in Transgenic Tobacco Plants (상처에 의해서 유도되는 벼 calmodulin promoter의 transgenic 담배에서조직 특이적 발현)

  • Choi Young Ju
    • Journal of Life Science
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    • v.15 no.2 s.69
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    • pp.176-181
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    • 2005
  • To study calmodulin (CaM) gene expression and its regulation, rice CaM promoter (RCaM-2) was isolated and fused to $\beta-glucuronidase$ (GUS), reporter gene. X-Glue staining patterns revealed that GUS localization is high in meristemic tissues such as the stem apex, stolen tip, and vascular regions. GUS staining in the transverse sections of stem and petiole was restricted to the inside of the vascular system, and cortex and epidermis located outside of the vascular system usually did not show GUS staining even a plant that expressed strong activity. GUS activity was found to be tissue specific expressed and exhibited a dramatic transient increase in response to wounding. These results suggest that the 5'-flanking region of RCaM gene regulates wound-inducible expression.

The Changes of Stifle Joint Fluid with Cranial Cruciate Ligament Rupture in Dogs (개에 있어서 전방십자인대 단열시 슬관절액의 변화)

  • Nam-soo, Kim
    • Journal of Veterinary Clinics
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    • v.20 no.4
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    • pp.443-448
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    • 2003
  • To determine whether localization of tartrate-resistant acid phosphatase (TRAP) and cathepsin K was associated with rupture of the cranial cruciate ligament (CCL) in dogs. Tissue specimens were obtained from 30 dogs with CCL rupture during surgical treatment, 8 aged normal dogs, and 9 young normal dogs that were necropsied for reasons unrelated to this study and unrelated to musculoskeletal disease. The cranial cruciate ligament was examined histologically. $TRAP^+$ cells and cathepsin $K^+$ cells were identified by histochemical staining and immunohistochemical staining respectively. TRAP and cathepsin $K^+$ were co-localized within the same cells principally located within the epiligamentous region and to a lesser extent in the core region of ruptured CCL. Localization of $TRAP^+$ cells (P < 0.05) and cathepsin $K^+$ cells (P =0.05) within CCL tissue was significantly increased in dogs with CCL rupture, compared with aged-normal dogs, and young normal dogs (P < 0.05 - TRAP, P < 0.001 - cathepsin K). Localization of $TRAP^+$ cells and cathepsin $K^+$ cells within the CCL tissue of aged-normal dogs was also increased compared with young normal dogs (P < 0.05). Small numbers of $TRAP^+$ cells and cathepsin $K^+$ cells were seen in the intact ligaments of aged-normal dogs, which were associated with ligament fasicles in which there was chondroid transformation of ligament fibroblasts and disruption of the organized hierarchical structure of the extracellular matrix. $TRAP^+$ cells and cathepsin $K^+$ cells were not seen in CCL tissue from young-normal dogs. Localization of the proteinases $TRAP^+$ and cathepsin $K^+$ in CCL tissue was significantly associated with CCL rupture. Small numbers of proteinase positive cells were also localized in the CCL of agednormal dogs without CCL rupture, but were not detected in CCL from young-normal dogs. Taken together, these findings suggest that the cell signaling pathways that regulate expression of these proteinases in CCL tissue may form part of the mechanism that leads to upregulation of collagenolytic ligament remodeling and progressive structural failure of the CCL over time.

Characteristics of Alkaline and Acid Phosphatase in Echinostoma hortense (호르텐스극구흡충에서 Alkaline Phosphatase 및 Acid Phosphatase의 특성)

  • 양용석;김인식;임지애;강성구;박주연
    • Biomedical Science Letters
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    • v.5 no.1
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    • pp.119-129
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    • 1999
  • This study was aimed to investigate the enzyme-histochemical localization and characteristics of alkaline and acid phosphatase extracted from adult of Echinostoma hortense. Using the Gomori calcium stain and the Gomori lead nitrate satin method, we found that the alkaline and acid phosphatases were localized mostly in the intestine, vitellaria and pharynx of Echinostoma hortense. The three isozymes of alkaline phosphatase and two isozymes of acid phosphatase were separated from Echinostoma hortense by electrophoresis. The isozymes of alkaline phosphatase were 145.9, 207.5, 220.8 kDa and the isozymes of acid phosphatase were 179.5 and 209.4 kDa. The activity of alkaline phosphatase was denatured completely after heating at 9$0^{\circ}C$ for 12 seconds. The optimum pH and temperature for activity of alkaline phosphatase were about pH 9 and 4$0^{\circ}C$, while the optimum pH for activity of acid phosphatase was about pH 5. The maximum activity of alkaline phosphatase was at 189 unit, but maximum activity of acid phosphatase was at 71 unit As the result from above, we observed that alkaline and acid phosphatases funtion mainly in the alimentary tract and vitellaria. Echinostoma hortense performs the parasitism in the intestine of host by using proper isozyme of phosphatase.

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Effects of Jasmonic Acid and Wounding on Polyphenol Oxidase Activity in Senescing Tomato Leaves

  • Jin, Sun-Young;Hong, Jung-Hee
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.4 no.4
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    • pp.231-240
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    • 2000
  • Effects of Jasmonic Acid and Wounding on Polyphenol Oxidase Activity in Senescing Tomato Leaves The effects of jasmonic acid(JA) and wounding on polyphenol oxidase(PPO) during leaf senescence was investigated by measuring the PPO activity in detached tomato(Lycopersicon esculentum Mill.) leaves of two-week-old seedlings. The PPO activity in the detached senescing leaves increased significantly in the dark. The leaf segments responded to the application of JA with accelerated senescence, as indicated by the loss of chlorophyll and rapid increase in the PPO activity. The senescence-promoting action of JA differed in the light and dark. Wounding the detached senescing leaves by scraping surface segments or making punctures with needles considerably delayed the loss of chlorophyll and had a significant effect on the PPO activity, the amounts of which were roughly proportional to the intensity of the wounding. In the dark, the combination of wounding plus JA resulted in stable levels of chlorophyll and PPO. JA and ABA acted similarly in both unwounded and wounded leaves, however, the amount of chlorophyll and PPO in the wounded segments was always higher than in the respective controls. JA was found to eliminate the senescence-retarding action of benzyladenine. In a histochemical localization test, the PPO activity was found to be localized in the cell walls of the parenchyma tissue, thereby indicating moderate cytoplasmic reactions. In the JA-treated plants, the PPO activity was intense in the cells of the cortex and phloem parenchyma. Accordingly, based on these observations it would appear that PPO is a component of a defense response maker, whereas JA plays an integral role in the intracellular signal transduction involved in inducible defense mechanisms.

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Overexpression of ginseng UGT72AL1 causes organ fusion in the axillary leaf branch of Arabidopsis

  • Nguyen, Ngoc Quy;Lee, Ok Ran
    • Journal of Ginseng Research
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    • v.41 no.3
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    • pp.419-427
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    • 2017
  • Background: Glycosylation of natural compounds increases the diversity of secondary metabolites. Glycosylation steps are implicated not only in plant growth and development, but also in plant defense responses. Although the activities of uridine-dependent glycosyltransferases (UGTs) have long been recognized, and genes encoding them in several higher plants have been identified, the specific functions of UGTs in planta remain largely unknown. Methods: Spatial and temporal patterns of gene expression were analyzed by quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) and GUS histochemical assay. In planta transformation in heterologous Arabidopsis was generated by floral dipping using Agrobacterium tumefaciens (C58C1). Protein localization was analyzed by confocal microscopy via fluorescent protein tagging. Results: PgUGT72AL1 was highly expressed in the rhizome, upper root, and youngest leaf compared with the other organs. GUS staining of the promoter: GUS fusion revealed high expression in different organs, including axillary leaf branch. Overexpression of PgUGT72AL1 resulted in a fused organ in the axillary leaf branch. Conclusion: PgUGT72AL1, which is phylogenetically close to PgUGT71A27, is involved in the production of ginsenoside compound K. Considering that compound K is not reported in raw ginseng material, further characterization of this gene may shed light on the biological function of ginsenosides in ginseng plant growth and development. The organ fusion phenotype could be caused by the defective growth of cells in the boundary region, commonly regulated by phytohormones such as auxins or brassinosteroids, and requires further analysis.

Histology and lectin histochemistry in the vomeronasal organ of Korean native cattle, Bos taurus coreanae

  • Jang, Sungwoong;Kim, Bohye;Kim, Joong-Sun;Moon, Changjong
    • Journal of Animal Reproduction and Biotechnology
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    • v.36 no.4
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    • pp.270-284
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    • 2021
  • The vomeronasal organ (VNO) is critical for reproduction and social behavior in ruminants, including cattle. The present study examined the structure of the VNO and its epithelial cells in neonatal and adult Korean native cattle (Hanwoo), Bos taurus coreanae, using immunohistochemistry and lectin histochemistry. Histologically, the VNO comprised two types of epithelia: medial vomeronasal sensory (VSE) and lateral vomeronasal non-sensory epithelia (VNSE). Numerous blood vessels and nerve bundles were observed within the vomeronasal cartilage encased lamina propria. Immunohistochemistry revealed high expression level of protein gene product9.5 and moderate expression level of olfactory marker protein in the neuroreceptor cells of the VSE and occasionally in some ciliated cells of the VNSE in both neonates and adults. The properties of the glycoconjugates in the VNO were investigated using 21 lectins, most of which were expressed at varied intensities in the VSE and VNSE, as well as in the lamina propria. Several lectins exhibited variations in their intensities and localization between neonatal and adult VNOs. This study is the first descriptive lectin histochemical examination of the VNO of Korean native cattle with a focus on lectin histochemistry, confirming that the VNO of Korean native cattle is differentiated during postnatal development.

Localization and characteristics of lactate and palate dehydrogenase in the sparganum and adult worm of Spirometra erinacei (만손열두조충에서 젖산탈수소효소와 말산탈수소효소의 분포와 특징)

  • Gwak, Gi-Hun;Jeon, Eun-U;Kim, Chang-Hwan
    • Parasites, Hosts and Diseases
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    • v.34 no.1
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    • pp.59-68
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    • 1996
  • This study investigated the enzyme histochemical localization and characteristics of lactate (LDH) and malate dehydrogenase (MDH) related with the oxidation-reduction metabolism in the sparganum and adult of 5. erinacei. By enzyme histochemical assay, activity of LDH was strong in the tegument and subtegumental muscle layers of the adult and sparganum. Activity of MDH was strong in the tegument of the sparganum and subtegumental muscle layers of the adult. However it was weak in the tegument of the adult. By electrophoresis, 45 kDa band was major and common in LDH of adults and spargana. The 150 kDa molecule was the major and common band in MDH of adults and r -spargana (from experimentally infected rats) . By isoelectrofocusing, isoelectric points (Pl) or 4 MDH isogyme from adult worm were 6.0.6.5, 6.7 and 7.1, respectively. Pl 6.0 was the major band. The active range of pH for MDH was about pH 6-8 and the optimum pH was pH 7 The effective temperature on the MDH was about $30^{\circ}C$$50^{\circ}C$ and the optimum temperature was about 40℃ in spargana md adult worm. In the stability against heat, when MDH was heated at 85℃ for 10 seconds, the activity was denatured perfectly. Maximum activity or MDH was 19.4 unit in the s-sparganum (from snakes), 24.5 unit in the r-sparganum (from rats) and 108.0 unit in the adult worm. The maximum activity was higher in adults than in spargana. The present result showed us that the nutrients absorbed through the tegument were transferred into inner tissues and were utilized as the source of metabolism. According to the habitat of the parasite, the isozymes of LDH and MDH are activated differently, and by this different activation the sparganum and adult can adapt themselves to parasitic circumstances.

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