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Application of the Alkaline Comet Assay for Detecting Oxidative DNA Damage in Human Biomonitoring (인체 산화적 DNA손상에 대한 Human Biomonitoring도구로서 Alkaline Comet Assay의 활용 가능성 연구)

  • 박은주;강명희
    • Journal of Nutrition and Health
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    • v.35 no.2
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    • pp.213-222
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    • 2002
  • The alkaline comet assay has been used with increasing popularity to investigate the level of DNA damage in biomonitoring studies within the last decade in Western countries. The purpose of this study was to evaluate the usefulness of the alkaline comet assay as a biomarker of oxidative DNA damage for monitoring in the Korean population, and also to evaluate the effect of nutritional status and lifestyle factors on H2O2 induced oxidative DNA damage measured by the alkaline comet assay in human lymphocytes. The study population consisted of 61 healthy Korean male volunteers, aged 20-28. Epidemiological background data including dietary habits, smoking habits and anthropometrical measurements were collected through personal interviews. After blood collection, the comet assay in peripheral lymphocytes and plasma lipids analysis was carried out and the results analyzed. Tail moment (TM) and tail length (TL) of the comet assay were use\ulcorner to measure DNA damage in the lymphocytes of the subjects. Statistically significant (p < 0.05) positive correlations were observed between DNA damage (TM or TL) and smoking habits expressed as cigarettes smoked per day and pack years (r = 0.311 and 0.382 for TM, r = 0.294 and 0.350 for TL, respectively). There were also significant positive correlations between DNA damage parameter and waist-hip ratio. Higher plasma triglyceride levels were associated with increased damage to DNA. There were no correlations between the consumption frequencies of vegetables and DNA damage to the subjects. However, consumption frequencies of fruit and fruit juice intake were inversely associated with the TM and TL. The results indicate that die comet assay is a simple, rapid and sensitive method for detecting lymphocyte DNA damage induced by cigarette smoking. Consumption of fruit or fruit juices could potentiall modify the damaged DNA in the human peripheral lymphocytes of young Korean men.

Changes of Obesity Index, Serum Lipid Profiles and Nutrient Intakes in Obese Children after the Weight Control Program of Nutrition Education (체중조절 프로그램을 통한 비만 아동의 비만도, 혈중지질 및 영양소섭취량의 변화)

  • Lee, Eun-Ju;Kim, Kyung-Hee
    • Korean Journal of Community Nutrition
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    • v.15 no.1
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    • pp.61-72
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    • 2010
  • The purpose of this study was to evaluate the effect of a weight control program on anthropometric values, serum lipid profiles and nutrient intakes. The subjects of this study were 38 obese children (boy : 17, girl : 21) with obesity index over 120%. The weight control program for obese children included nutritional education (50 min) and exercise (50 min) for 10 weeks. The nutrition intakes of the children were surveyed before and after the weight control program by 24hr recall test. The BMI, R$\ddot{o}$hrer Index, Obesity Index, WHR (Waist - Hip ratio) and body fat(%) were significantly decreased after completion of the weight control program. The total cholesterol and HDL-cholesterol of subjects were significantly increased after the weight control program. Distribution of serum lipid profiles was slightly changed. The energy intakes were significantly decreased (p < 0.001). from 1760.8 kcal to 1435.2 kcal. In addition, the intakes of P, Zn, retinol, vitamin $B_1$, vitamin $B_2$, vitamin E and niacin were significantly decreased. while intakes of vitamin C and folate were increased. Calcium and folic acid were upper 25% of subjects, under EAR(Estimated Average Requirements) intake before the weight control program. The distribution of energy intakes was significantly changed into positive status; fat percentage was decreased 26.3% to 22.1% (p < 0.01). Carbohydrate was increased 58.6% to 61.2% (p < 0.05). Meal distribution of energy intakes was changed; calorie percentage from lunch significantly increased from 32.2% to 38.3%. Calorie percentage from snack significantly decreased from 17.7% to 13.5%. In conclusion, weight control program for 10 weeks is effective in obesity index and nutrient intakes although serum lipid values were a little changed.

방전 플라즈마 소결 공법을 이용한 FSW-Tool 용 $WC-5Mo_2C-5Co$ 소결체 제조와 기계적 특성 평가

  • Yun, Hui-Jun;Park, Hyeon-Guk;Lee, Seung-Min;Bang, Han-Seo;Bang, Hui-Seon;O, Ik-Hyeon
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2011.10a
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    • pp.40.2-40.2
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    • 2011
  • 초경합금은 경도가 높은 재료를 말하며 일반적으로는 탄화텅스텐(WC)계 재료를 말한다. 국내 현재 초경합금 동향은 반도체 산업, 내마모성 공구, 절삭공구, 금형 등 많은 분야에 사용되어지고 있다. 또한 최근 들어 FSW (Friction Stir Welding, FSW)기술이 발전함에 따라 접합기술개발이 다양화되면서 FSW Tool의 고성능의 초경 재료가 요구되어지며 장수명의 Tool개발이 되어야 한다. 국내에서는 초경 합금 재료로 사용되어지고 있는 텅스텐 카바이드(WC)와 코발트(Co)를 이용하여 많은 연구가 진행되었다. 본 실험에서는 텅스텐 카바이드와 코발트 및 몰르브덴 카바이드를 혼합하여 소결체를 제조하였다. 실험에 사용된 텅스텐 카바이드는 높은 경도를 가지고 강한 취성을 나타내며, 소결에 어려운 단점이 있다. 이러한 단점을 코발트와 몰리브덴 카바이드를 첨가하여 소결온도를 낮춰주는 역할과 액상 소결시 텅스텐카바이드 입자사이에 침투하여 액상소결에 의한 치밀화가 가능하게 해주며 인성이 향상되어 고인성 재료를 만들 수 있었다. 본 실험에서는 합성과 치밀화가 동시에 진행되는 SPS (Spark Plasma Sintering:SPS) 장비를 이용하여 실험을 진행하였다. 이 방법은 방전플라즈마 소결 공법으로, 기존의 연소법과 열간 가압기술(Hot-press, HIP)을 결합한 방식으로 단 시간, 단일공정으로 치밀한 소결체를 얻을 수 있는 장점이 있다. 본 연구에서는 $WC-5Mo_2C$-5wt%Co 소결체 제조를 위해 원소 분말을 Horizontal ball milling 혼합하였다. 균일하게 혼합된 분말을 흑연다이에 충진하여 펄스전류와 기계적 압력을 동시에 가하여 $WC-5Mo_2C-5Co$ 복합재료를 제조하고 소결체의 밀도, 순도, 상변태, 미세조직 등을 분석 및 평가하였다. SPS공정 조건은 고진공하에서 $1,200^{\circ}C$-60MPa, 펄스비 12:1 조건으로 수행하였으며, 얻어진 $WC-5Mo_2C-5Co$ 소결체의 상대 밀도는 98%이상 이였다. 또한, 결정립 크기는 약 400 nm였으며, 경도는 $2,453kg/mm^2$를 나타내었다.

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Fatigue Phenomenon of Mechanical Properties in Jean Slacks by the after Wearing (진바지의 착용 후 부위별 피로도에 관한 연구)

  • Lee, Chang-Mi;Kim, Tae-Gyu;Gwon, O-Gyeong
    • Journal of the Korean Society of Clothing and Textiles
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    • v.22 no.2
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    • pp.257-266
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    • 1998
  • This study was conducted to examine the changes of the mechanical properties of the position in jean slacks during the wear, previously used for the wearing test, which were subjected to repeated tensile-shearing deformation using a simulated fatigue tester has been investigated and compared, by calculating both mechanical properties and hand value(HV) of these fabrics with KES-F system and the by obtaining the THV through these calculated properties. The results are as follows. 1. The fatigue phenomenon of mechanical properties was the LT, 2H B, 2HBS, MMD, SMD, RC values increased, elasticity values of tensile, bending and shearing properties, such as B, G and compression properties LC, WC were reduced. It was shown, then, that those fabrics lost their elasticity and became flexible and soft with the increase of fatigue. 2. The hand value and THV; except anterior knee from all part of KOSHI, NUMERI, FUKURAMI was decreased. 3. The fatigue phenomenon of hand value was different on the position of clothing; on the position of hip, rate of B, G smaller than other parts and KOSHI was decreased, on the part of anterior thigh was FUKURAMI was increased, on the anterior knee RC, NUMERI, THV was increased, on the posterior knee was 2HB, 2HG, 2HGS showed increasedgreater than any other part and on the hem of back, MMD was increased, but NUMERI was decreased. 4. The changing process of mechanical properties in the simulation testing by the fatigue tester has similar tendency to that of the wearing tester. It is concluded that this testing method is useful to predict the fatigue phenomena of fabrics caused by wearing.

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An Analysis of Hanliu Phenomenon on the Chinese Street Fashion Style (중국의 스트리트 패션에 나타난 한류현상 분석)

  • Park, Kil-Soon
    • Korean Journal of Human Ecology
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    • v.13 no.6
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    • pp.967-983
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    • 2004
  • The purpose of this study is to review Hanliu phenomenon, a kind of social and cultural phenomenon, in China and to analyze its effects on the fashion style of new young generation of China. In this study, Hanliu phenomenon means the enthusiasm of Asian people for Korean mass culture including Korean dramas, pop songs, and fashions from late 1990s. This research adopts two kinds of methods for analyzing the phenomenon: qualitative and quantitative research methods. As a qualitative research method, we analyzed it with several sources of documentaries and audio-visual materials: articles from newspapers and magazines, special TV reports, and documentary movie files from Internet. As a quantitative research method, we surveyed approximately 100 female students of Beijing university and asked how they feel Korean culture and fashions. The Hanliu phenomenon led to the popularity of Korean products as well as general culture of Korea. Also, it influenced Chinese young generation so much that Korean fashion has become prevailing. Such influence on the street fashion of Chinese youths can be summarized in three factors as follows: First, Korean entertainers' fashion is widely imitated. For example, H.O.T-like hairstyles, hip-hop styles, large heel shoes with boots-cut pants, and long-curled permanent hairstyles have been on among Chinese youths. Second, the preference for Korean fashion products has highly increased. The number of stores dealing with Korean fashion products has increased. Even the 'Kim Hee Seen,' a fashion brand named after a famous Korean actress, was introduced. Finally, Korean culture and products have widely been imitated in China as much as the increasing popularity of Korean fashion products. This study reveals that Hanliu phenomenon is widespread in China, and Chinese youths are largely affected by the fashion styles of Korean entertainers. Also, Korean fashion products are largely imitated and benchmarked in China. Hanliu phenomenon is a big chance to approach the fashion market of China, the largest buying power in the world. To make inroads into the Chinese fashion market, we suggest that we need to have our own brand and to make the most of culture, stars, and Internet in marketing. Also, we need a well-planned strategy for a success in the Chinese fashion market.

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인공관절의 수명 향상을 위해 PIII&D (Plasma Immersion Ion Implantation & Deposition) 기술로 제조된 인공관절용 NbN 박막의 마모 특성 평가

  • Park, Won-Ung;Jeon, Jun-Hong;Mun, Seon-U;Choe, Jin-Yeong;Im, Sang-Ho;Han, Seung-Hui
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.08a
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    • pp.189-189
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    • 2011
  • 인공관절은 노인성 질환이나 자가 면역질환, 신체적인 외상 등으로 인하여 발생하는 관절의 손상 부위를 대체하기 위해 고안된 관절의 인공 대용물이다. 인공 관절 중 인공 고관절의 경우 관절 운동을 하는 라이너(Liner)와 헤드(Head) 부분이 인공관절의 수명을 결정하게 되는데, 헤드 부분에 메탈소재와 라이너 부분에 고분자 소재를 사용하는 MOP (metal on polymer) 구조의 인공관절은 충격흡수의 장점이 있는 반면 wear debris에 의한 골용해로 인하여 관절이 느슨해지는 문제점이 발생하여 재 시술의 주요 원인이 되고 있다. 또한 메탈 헤드의 마모로 인한 금속이온의 용출은 세포 독성의 문제를 야기하여 인공관절의 수명을 낮추는 또 하나의 요인이 되고 있다. 따라서 인공관절의 수명을 늘리기 위해 DLC, ZrO, TiN 등의 높은 경도 값을 갖는 박막을 금속 헤드 위에 증착하여 상대재인 인공관절용 고분자 소재의 마모량을 줄이고자 하는 연구가 활발하게 진행 되고 있다. 본 연구에서는 PIII&D (Plasma Immersion Ion Implantation & Deposition)공정을 이용하여 Co-Cr-Mo 합금 소재 niobium nitride (NbN) 박막을 증착하여 상대제인 UHMWPE (ultra high molecular polyethylene)의 마모를 줄이고자 하는 연구를 진행하였다. 마모량을 감소시키기 위하여, 박막 증착전에 질소를 이온주입하는 pre-ion implantation 공정을 도입하였으며, 또한 Co-Cr 합금과 NbN박막 사이의 접착력을 증가시키기 위하여 박막의 증착 초기에 이온주입과 증착을 동시에 수행하는 dynamic ion mixing공정을 수행하였다. NbN 박막의 특성을 평가하기 위해 XRD, XPS, AFM 등의 분석을 수행하였으며, 상대재인 초고분자량 폴리에틸렌의 마모량을 측정하기 위해 Pin-on-disk tester를 이용하여 마모 실험을 진행하였다. 마모 실험 결과, pre-ion implantation 공정을 도입한 경우 현재 상용화 되어있는 Co-Cr 합금에 비하여 마모량을 2배 이상 감소시키는 것을 확인 할 수 있었으며, dynamic ion mixing 공정을 도입한 경우 장시간의 마모 시험에 대한 마모 특성이 향상 되는 것을 확인 할 수 있었다.

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A Study on the Travel Fashion Bag Design Using the Op-Art Textile Patterns -Applying the Artwork of Victor Vasarely- (옵아트(Op-Art) 패턴의 여행용 패션가방 디자인 연구 -빅토르 바자렐리(Victor Vasarely)의 작품 응용을 중심으로-)

  • Kim, Min-Hye;Park, Hye-Won
    • Journal of the Korean Society of Clothing and Textiles
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    • v.35 no.3
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    • pp.371-384
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    • 2011
  • This study with 'trip' as a theme, aims to develop textile designs and fashion bags for travel which is widely applicable fitting traveler's T P O, applying Victor Vasarely's artwork. During the planning process of design, 'Whenever & Wherever's concept was set by analyzing fashion trend information in 2010-11 F/W proposed by Interfashion Planning and FCK; in addition, 'Bobos' were selected as research targets. It organized 4 images of 'Modern', 'Romantic', 'Classic' and 'Fantastic'. The motives are proposed textile design with '$Cross^2$', 'Falling Z', 'Cir-Classicism' and 'Skinny-holic' per each theme. The designs developed were printed out and into polyester canvas with a Polaris V6 DTP from dgen, Corp. Textile designs developed were applied for 'two-way' formed fashion bags for traveling with high practicality. Fashion bags for traveling consisted of big bags, tote bags, and hip sacks; a total of 12 works (4 sets per theme) were produced. This study sought practical plans for artworks by producing fashion goods through a DTP system. The results of this study can be used as guidelines for further studies and as a significant contribution to the creation of high values for exciting fashion products.

The Kinematic Analysis on the Instep Shooting Motion of Female High School Soccer Players According to the Angles of Approach (접근각도에 따른 여자고등학교 축구선수의 인스텝 슈팅 동작에 관한 운동학적 분석)

  • Cho, Kyu-Kwon;Kim, You-Sin;Choi, Gil-Soon
    • Korean Journal of Applied Biomechanics
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    • v.16 no.2
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    • pp.153-163
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    • 2006
  • The purpose of this research was to analyze kinematic variables that appear during the instep shooting motion of female high school soccer players according to the angle of approach to find effective shooting motions. For this experiment, 5 female high school soccer players from the K city were participated in this study as the subject group, and as a through comparison and analysis of the resulting numbers of the variables, we came to the following conclusions. 1) Stride length and stride length/lower extremity length increased as the angle of approach increased. 2) As for C.O.G movement displacement, it was highest at an approach angle of $90^{\circ}$ during Right Foot Contact, at $135^{\circ}$ during Left Foot Contact, at $0^{\circ}$ during Rigth Toe Top, at $45^{\circ}$ during Impact, and at $0^{\circ}$ during Follow through. 3) The time required for each phase was longest at APP and shortest at BSP. The time required increased a little as the angle of approach increased, and the total time required also increased as the angle of approach increased. 4) The angle of the ankle joint was largest at an approach angle of $45^{\circ}$ for all events except Right Foot Contact. 5) The angle of the knee joint was largest at an approach angle of $135^{\circ}$ during Right Foot Contact, at $0^{\circ}$ during Left Foot Contact, at $45^{\circ}$ during Right Toe Top, at $135^{\circ}$ during Impact, and at $90^{\circ}$ during Follow through. 6) The angle of the hip joint was largest at an approach angle of $90^{\circ}$ during Right Foot Contact, at$0^{\circ}$ during Left Foot Contact, at $0^{\circ}$ during Right Toe Top, at $90^{\circ}$ during Impact, and at $0^{\circ}$ during Follow through.

The Kinematical Analysis of the Over Head Kick in Soccer (축구 오버헤드 킥 동작의 운동학적 분석)

  • Kim, Eui-Hwan;Lee, Yo-Yeoul;Kim, Sung-Sup;Kwon, Mook-Seok;Kim, Sung-Ho
    • Korean Journal of Applied Biomechanics
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    • v.13 no.1
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    • pp.155-171
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    • 2003
  • The purpose of this study was to analyze the kinematic variables of over head kick(OHK) in soccer with three dimensional analysis technique and show the kinematic characteristics of it. The 7 subjects were university football player who have been playing football more than 7 years. The OHK was filmed on 16mm video camera(30frame/sec.) kinematic variables were temporal, postures, and COG(center of gravity). The mean values and the standard deviation for each variables were obtained and used as basic factors for examining characteristics of OHK. The results of this analysis were as follows : Temporal variables : The total time elapsed(TE) of OHK was $0.95{\sim}1.14sec$, the 1st phase was 0.35sec., 2nd phase was 0.46sec., and 3rd phase was 0.22sec.. Posture variables : When subjects performed OHK at the impact event, the ankle and knee angle of kicking foot were more extend than supporting foot. but the hip angle of supporting foot were more extend than kirking foot. Moving distance of the center of mass of the both foot. When subject performed OHK at the impact event, the range of distance on mediolateral direction aspect into right left shoulder line, anteroposterior direction aspect was $20.9{\pm}10.5cm$, vertical direction aspect was $92.3{\pm}19.9cm$. Angular velocity : the faster angular velocity of knee ankle on the kicking foot pew form jump position to landing position, the faster velocity of ball became. C. O. G. variables. When subject performed OHK at the impact event, upper part of the body was getting lower, lower part of the body was getting higher.

Effect of Wearing Ankle Weights on Underwater Treadmill Walking

  • Park, Que Tae;Kim, Suk Bum;O'Sullivan, David
    • Korean Journal of Applied Biomechanics
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    • v.29 no.2
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    • pp.105-112
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    • 2019
  • Objective: The main purpose of this study was to investigate the effects of wearing an ankle weight belt while performing gait in water by focusing on the effect of using ankle weights have on the gait kinematics and the muscle activities for developing optimum training strategies. Method: A total of 10 healthy male university students were recruited for the study. Each participant was instructed to perform 3 gait conditions; normal walking over ground, walking in water chest height, and walking in water chest height while using ankle weights. All walking conditions were set at control speed of $4km/h{\pm}0.05km/h$. The depth of the swimming pool was at 1.3 m, approximately chest height. The motion capture data was recorded using 6 digital cameras and the EMG was recorded using waterproof Mini Wave. From the motion capture data, the following variables were calculated for analysis; double and single support phase (s), swing phase (s), step length (%height), step rate (m/s), ankle, knee, and hip joint angles ($^{\circ}$). From the electromyography the %RVC of the lower limb muscles medial gastrocnemius, rectus femoris, erector spinae, semitendinosus, tibialis anterior, vastus lateralis oblique was calculated. Results: The results show significant differences between the gait time, and step length between the right and left leg. Additionally, the joint angular velocities and gait velocity were significantly affected by the water resistance. As expected, the use of the ankle weights increased all of the lower leg maximum muscle activities except for the lower back muscle. Conclusion: In conclusion, the ankle weights can be shown to stimulate more muscle activity during walking in chest height water and therefore, may be useful for rehabilitation purposes.