• Korean Journal of Ecology and Environment
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• v.39 no.1 s.115
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• pp.52-61
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• 2006

The aim of this study was to analyze the seasonal patterns of zooplankton community dynamics in the lower Nakdong River (Mulgum, RK; river kilometer; 27 km from the estuarine barrage), with a Self-Organizing Map (SOM) based on weekly sampled data collected over ten years(1994 ${\sim}$ 2003). It is well known that zooplankton groups had important role in the food web of freshwater ecosystems, however, less attention has been paid to this group compared with other community constituents. A non-linear patterning algorithm of the SOM was applied to discover the relationship among river environments and zooplankton community dynamics. Limnological variables (water temperature, dissolved oxygen, pH , Secchi transparency, turbidity, chlorophyll a, discharge, etc.) were taken into account to implement patterning seasonal changes of zooplankton community structures (consisting of rotifers, cladocerans and copepods). The trained SOM model allocated zooplankton on the map plane with limnological parameters. Three zooplankton groups had high similarities to one another in their changing seasonal patterns, Among the limnological variables, water temporature was highly related to the zooplankton community dynamics (especially for cladocerans). The SOM model illustrated the suppression of zooplankton due to the increased river discharge, particularly in summer. Chlorophyll a concentrations were separated from zooplankton data set on the map plane, which would intimate the herbivorous activity of dominant grazers. This study introduces the zooplankton dynamics associated with limnological parameters using a nonlinear method, and the information will be useful for managing the river ecosystem, with respect to the food web interactions.

• Proceedings of the Korea Water Resources Association Conference
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• 2002.05a
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• pp.43-50
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• 2002

Accurate quantitative forecasting of rainfall for basins with a short response time is essential to predict streamflow and flash floods. Previously, neural networks were used to develop a Quantitative Precipitation Forecasting (QPF) model that highly improved forecasting skill at specific locations in Pennsylvania, using both Numerical Weather Prediction (NWP) output and rainfall and radiosonde data. The objective of this study was to improve an existing artificial neural network model and incorporate the evolving structure and frequency of intense weather systems in the mid-Atlantic region of the United States for improved flood forecasting. Besides using radiosonde and rainfall data, the model also used the satellite-derived characteristics of storm systems such as tropical cyclones, mesoscale convective complex systems and convective cloud clusters as input. The convective classification and tracking system (CCATS) was used to identify and quantify storm properties such as life time, area, eccentricity, and track. As in standard expert prediction systems, the fundamental structure of the neural network model was learned from the hydroclimatology of the relationships between weather system, rainfall production and streamflow response in the study area. The new Quantitative Flood Forecasting (QFF) model was applied to predict streamflow peaks with lead-times of 18 and 24 hours over a five year period in 4 watersheds on the leeward side of the Appalachian mountains in the mid-Atlantic region. Threat scores consistently above .6 and close to 0.8 ∼ 0.9 were obtained fur 18 hour lead-time forecasts, and skill scores of at least 4％ and up to 6％ were attained for the 24 hour lead-time forecasts. This work demonstrates that multisensor data cast into an expert information system such as neural networks, if built upon scientific understanding of regional hydrometeorology, can lead to significant gains in the forecast skill of extreme rainfall and associated floods. In particular, this study validates our hypothesis that accurate and extended flood forecast lead-times can be attained by taking into consideration the synoptic evolution of atmospheric conditions extracted from the analysis of large-area remotely sensed imagery While physically-based numerical weather prediction and river routing models cannot accurately depict complex natural non-linear processes, and thus have difficulty in simulating extreme events such as heavy rainfall and floods, data-driven approaches should be viewed as a strong alternative in operational hydrology. This is especially more pertinent at a time when the diversity of sensors in satellites and ground-based operational weather monitoring systems provide large volumes of data on a real-time basis.

• Journal of Microbiology and Biotechnology
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• v.18 no.8
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• pp.1386-1392
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• 2008

A gene (sll0158) putatively encoding a glycogen branching enzyme (GBE, E.C. 2.4.1.18) was cloned from Synechocystis sp. PCC6803, and the recombinant protein expressed and characterized. The PCR-amplified putative GBE gene was ligated into a pET-21a plasmid vector harboring a T7 promoter, and the recombinant DNA transformed into a host cell, E. coli BL21(DE3). The IPTG-induced enzymes were then extracted and purified using Ni-NTA affinity chromatography. The putative GBE gene was found to be composed of 2,310 nucleotides and encoded 770 amino acids, corresponding to approx. 90.7 kDa, as confirmed by SDS-PAGE and MALDI-TOF-MS analyses. The optimal conditions for GBE activity were investigated by measuring the absorbance change in iodine affinity, and shown to be pH 8.0 and $30^{\circ}C$ in a 50 mM glycine-NaOH buffer. The action pattern of the GBE on amylose, an $\alpha$-(1,4)-linked linear glucan, was analyzed using high-performance anion-exchange chromatography (HPAEC) after isoamylolysis. As a result, the GBE displayed $\alpha$-glucosyl transferring activity by cleaving the $\alpha$-(1,4)-linkages and transferring the cleaved maltoglycosyl moiety to form new $\alpha$-(1,6)-branch linkages. A time-course study of the GBE reaction was carried out with biosynthetic amylose (BSAM; $M_p{\cong}$8,000), and the changes in the branch-chain length distribution were evaluated. When increasing the reaction time up to 48 h, the weight- and number-average DP ($DP_w$ and $DP_n$) decreased from 19.6 to 8.7 and from 17.6 to 7.8, respectively. The molecular size ($M_p$, peak $M_w{\cong}2.45-2.75{\times}10^5$) of the GBE-reacted product from BSAM reached the size of amylose (AM) in botanical starch, yet the product was highly soluble and stable in water, unlike AM molecules. Thus, GBE-generated products can provide new food and non-food applications, owing to their unique physical properties.

• Geotechnical Engineering
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• v.14 no.5
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• pp.39-52
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• 1998

The pupose of the present paper is to estimate the effects of constraint condition of pile head, ground condition(dry unit weight. $\gamma_4$) and embedded pile lengths on the behavior of single pile which is embedded in normally consolidated clay. BBperiment functions can be quantified to these effects obtained from the results of model teats. The ground of model tests is normally consolidated( NC ) clay under three kinds of effective vertical stress. The results of the model tests using the steel pile of two different embedded pile length and of free-head and fired-head show that the lateral load-deflection relationship is to be elasto plastic behavior below $\gamma_d/\gamma_{dmax}$: 0.84 and that the reduction of lateral load of beyond maximum lateral load($Q_{max}$) at each model test is significantly time-dependent. In this study, it is shown that the displacement relationship can be fitted to exponential function of time by model best results. The effect of ground conditions on the ultimate and yield lateral load is fitted to exponential function including the ratio of dry unit weight to maximum dry unit weight. When tests by results are compared with those from Broms and Budhu et at., the predicted results are over-estimated about 27-87 ayo. In effectivity of constraint condition of pile head on the lateral load-deflection response, the $Q_{fixed}/Q_{gree}-y/D$ relationship is highly non-linear and fitted to parabolic function.

• Journal of Advanced Marine Engineering and Technology
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• v.41 no.3
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• pp.202-208
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• 2017

The recently developed marine engines for propulsion of ships have higher torsional exciting force than previous engines to improve the propulsion efficiency and to reduce specific fuel oil consumption. As a result, a viscous damper or viscous-spring damper is installed in front of marine engine to control the torsional vibration. In the case of viscous damper, it is supposed that there is no elastic connection in the silicon oil, which is filled between the damper housing and inertia ring. However, In reality, the silicon oil with high viscosity possesses torsional stiffness and has non-linear dynamic characteristics according to the operating temperature and frequency of the viscous damper. In this study, the damping characteristics of a viscous damper used to control the torsional vibration of the shafting system have been reviewed and the characteristics of torsional vibration of the shafting system equipped with a corresponding viscous damper have been examined. In addition, it is examined how to interpret the theoretically optimal dynamic characteristics of a viscous damper for this purpose, and the optimum design for the propulsion shafting system has been suggested considering the operating temperature and aging. when the torsional vibration of the shafting system is controlled by a viscous damper filled with highly viscous silicon oil.

• Analytical Science and Technology
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• v.30 no.6
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• pp.348-354
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• 2017

In this study, patterns of proteome expression were monitored and specifically expressed proteins in human milk were detected in collected human milk after 1 week, 3 weeks, and 6 weeks from delivery. A quantitative shotgun proteomic approach was used to identify human milk proteins and reveal their relative expression amounts. For each sample, two independent human milk samples from two mothers were pooled, and then three replicated shotgun proteomic analyses were carried out. Casein, which is a highly abundant protein in human milk, was removed, and then trypsin was treated to produce a digested peptide mixture. The peptides were loaded in the home-made reversed-phase C18 fused-silica capillary column, and then the eluted peptides were analyzed by using a linear ion-trap mass spectrometer. The relative quantitation of proteins was performed by the normalized spectral count method. For each sample, 81-109 non-redundant proteins were identified. The identified proteins consisted of glycoproteins, metabolic enzyme, and chaperon enzymes such as lactoferrin, carboxylic ester hydrolase, and clusterin. The comparative analysis for the 63 proteins, which were reproducibly identified in all three replications, revealed that 25 proteins were statically significant differentially expressed. Among the differentially expressed proteins, Ig lambda-7 chain C region and tenascin drastically decreased with the delivery time.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.40 no.11
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• pp.83-94
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• 2003

In this paper. a highly linear and low glitch CMOS current mode digital-to-analog converter (DAC) by self calibration bias circuit is proposed. The architecture of the DAC is based on a current steering 6＋4 segmented type and new switching scheme for the current cell matrix, which reduced non-linearity error and graded error. In order to achieve a high performance DAC . novel current cell with a low spurious deglitching circuit and a new inverse thermometer decoder are proposed. The prototype DAC was implemented in a 0.35${\mu}{\textrm}{m}$ n-well CMOS technology. Experimental result show that SFDR is 60 ㏈ when sampling frequency is 32MHz and DAC output frequency is 7.92MHz. The DAC dissipates 46 mW at a 3.3 Volt single power supply and occupies a chip area of 1350${\mu}{\textrm}{m}$ ${\times}$750${\mu}{\textrm}{m}$.

• Cross-Cultural Studies
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• v.28
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• pp.17-36
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• 2012

This essay places the South Korean film Thirst (2009) within Park Chan-wook's oeuvre as a filmmaker notorious for graphic depictions of violence and revenge. Park's use of dark humour in his films, which is emphasized in Thirst perhaps more than ever, allows for a more self-aware depiction of violence, where both the viewer and the protagonist are awakened to the futility of revenge. This ultimately paints his characters as fascinatingly crazy - simultaneously heroes, villains, and victims. Film theorist Wes D. Gehring's three themes of dark humour ('man as beast,' 'the absurdity of the world,' and 'the omnipresence of death') become most obvious in Park's most recent film, which pays closer attention to character development through narrative detail. Rather than portraying the characters as sentimental, dark humour depicts their misfortunes in an alternative way, allowing for consideration of such taboo subjects as religion, adultery, and death/suicide. These issues are further tackled through Thirst's portrayal of its vampire protagonist, which ultimately de-mystifies the traditional vampire figure. While this character has more often been associated with romance, exoticism and the mystical powers of the supernatural, Thirst takes relatively little from the demons of Nosferatu (Murnau, 1922) and various other Dracula adaptations, nor the romantic figures of Interview with the Vampire (Jordan, 1994), and Twilight (Hardwicke, 2008). Instead, it is part of a much smaller group of contemporary vampire films, which are rather informed by a postmodern reconfiguration of the monster. Thus, this paper examines Thirst as an important contribution to the global and hybrid nature of those films in which postmodern vampires are sympathetic and de-mystified, exhibiting symptoms stemming from a natural illness or misfortune. Park's undertaking of a vampire film allows for a complex balance between narrative and visuals through his focus on the Western implications of this myth within Korean cinema. This combination of international references and traditional Korean culture marks it as highly conscious of New Korean Cinema's focus on globalization. With Thirst, Park successfully unites familiar images of the vampire hunting and feeding, with more stylistically distinct, grotesque images of violence and revenge. In this sense, dark humour highlights the less charming aspects of the vampire struggling to survive, most effective in scenes depicting the protagonist feeding from his friend's IV in the hospital, and sitting in the sunlight, slowly turning to ash, in the final minutes of the film. The international appeal of Park's style, combining conventions of the horror/thriller genre with his own mixture of dark humour and non-linear narrative, is epitomized in Thirst, which underscores South Korea's growing global interest with its overt international framework. Furthermore, he portrayal of the vampire as a sympathetic figure allows for a shift away from the conventional focus on myth and the exotic, toward a renewed construction of the vampire in terms of its contribution to generic hybridization and cultural adaptation.

• Journal of the Earthquake Engineering Society of Korea
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• v.27 no.1
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• pp.25-35
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• 2023

Considering the non-linear behavior of structure and soil when evaluating a nuclear power plant's seismic safety under a beyond-design basis earthquake is essential. In order to obtain the nonlinear response of a nuclear power plant structure, a time-domain SSI analysis method that considers the nonlinearity of soil and structure and the nonlinear Soil-Structure Interaction (SSI) effect is necessary. The Boundary Reaction Method (BRM) is a time-domain SSI analysis method. The BRM can be applied effectively with a Perfectly Matched Layer (PML), which is an effective energy absorbing boundary condition. The BRM has a characteristic that the magnitude of the response in far-field soil increases as the boundary interface of the effective seismic load moves outward. In addition, the PML has poor absorption performance of low-frequency waves. For this reason, the accuracy of the low-frequency response may be degraded when analyzing the combination of the BRM and the PML. In this study, the accuracy of the analysis response was improved by adjusting the PML input parameters to improve this problem. The accuracy of the response was evaluated by using the analysis response using KIESSI-3D, a frequency domain SSI analysis program, as a reference solution. As a result of the analysis applying the optimal PML parameter, the average error rate of the acceleration response spectrum for 9 degrees of freedom of the structure was 3.40%, which was highly similar to the reference result. In addition, time-domain nonlinear SSI analysis was performed with the soil's nonlinearity to show this study's applicability. As a result of nonlinear SSI analysis, plastic deformation was concentrated in the soil around the foundation. The analysis results found that the analysis method combining BRM and PML can be effectively applied to the seismic response analysis of nuclear power plant structures.

• Journal of the Korean Geotechnical Society
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• v.26 no.6
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• pp.57-70
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• 2010

Recent earthquakes have demonstrated that the tunnels, which were once considered to be highly resistant to earthquakes, are susceptible to substantial damage under severe seismic loading. Among various modes of deformation under an earthquake loading, the response of the tunnel in the transverse direction is known to be the critical mode. This paper investigates the seismic response of the tunnel in the transverse direction using the method of seismic displacement, which is a type of pseudo-static analysis. Firstly, the methods of calculating the ground deformation are compared. It is shown that the single and double cosine may not provide an accurate estimation of the ground deformation, and that a one-dimensional site response analysis needs to be performed for a more reliable evaluation. Secondly, the tunnel responses are calculated using the simplified, analytical, and numerical solutions. It is demonstrated that the simplified method provides poor estimates of the tunnel response ground deformation. The analytical solution is shown to be effective in modeling circular tunnels in uniform ground, but has serious limitation in modeling tunnel response in non-uniform ground. Numerical analyses are shown to be applicable to all cases, and give the most accurate estimates of the tunnel response. It is also demonstrated that the linear solutions can be so conservative that the soil nonlinearity needs to be accounted for more accurate evaluation of the tunnel response.