• Title/Summary/Keyword: higher fungi

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Antifungal Activity of Methyl 2-Benzimidazole Carbamate

  • Kim, Mal-Nam;Park, Hye-Young
    • Mycobiology
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    • v.31 no.2
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    • pp.81-85
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    • 2003
  • Antifungal properties of methyl 2-benzimidazole carbamate(BMC) were investigated using 16 fungi. Cytotoxicity test of BMC revealed that the morphology of HeLa cells was considerably deformed even at the concentrations as low as 0.1 ppm. Minimum inhibitory concentration(MIC) values of BMC for 7 fungi among the 16 tested ones were lower than $1.95{\times}10^{-4}{\mu}g/ml$, while Aspergillus flavus showed an MIC value higher than 1.0 ${\mu}g/ml$. Tolerance induction against BMC was successful only for Paecilomyces farinosus LAR10, contrary to the expectation that tolerance would be induced for the fungi having high MIC values such as Aspergillus niger ATCC 9642 and A. flavus ATCC 9643. Spore germination of A. niger ATCC 9642 was suppressed by BMC. However the mycelial growth of the fungus once germinated was not retarded at all by BMC up to 8 MIC. Addition of lanosterol provided a remedy for the reduced germination rate of A. niger ATCC 9642 spores.

Notes on the Korean Rotten Wood Fungi(I) (한국산부후균의 기재(I))

  • 조덕현
    • Korean Journal of Plant Resources
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    • v.9 no.2
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    • pp.97-104
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    • 1996
  • Many rotten wood fungi were collected at Mt.Moak Provincial Park(Chonlabuk-Do), Mt.Manduck(Chonlabuk-Do) and Byunsan Pennisula National Park from April, 1995 to October, 1995. These higher fungi were identified. According to the results, genera of Hypoderma, Piloderma and Cylidrobasidium are newly to korea. Following species are newly to Korea : Polyporus tuberaster, Phellinus ferruginosus, Corticium bomycinum, Phlebia rufa, Hyphoderma puberum, Piloderma byssinum, Cylinddrobasidium evolens and Peniophora pini.

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Antifungal Activity of Plumbagin Purified from Leaves of Nepenthes ventricosa x maxima against Phytopathogenic Fungi

  • Shin, Kwang-Soo;Lee, Sam-Keun;Cha, Byeong-Jin
    • The Plant Pathology Journal
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    • v.23 no.2
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    • pp.113-115
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    • 2007
  • A kind of naphthoquinone, plumbagin was purified and identified from the leaves of Nepenthes $ventricosa\;{\times}\;maxima$ through solvent extraction, silica gel column chromatography, and recrystallization. The yield (0.51%) was higher than that of the root of Plumbago scandens (0.26%), P. capensis (0.15%), and N. thorelii (0.092%). It exhibited antifungal activity against all plant pathogenic fungi tested, Alternaria alternata, Aspergillus niger, Bipolaris oryzae, Fusarium oxysporum, Phytophthora capsici, Rhizoctonia solani, Rhizopus stolonifer var. stolonifer and Sclerotinia sclerotiorum. The minimum inhibitory concentration values ranged from about 4.8 to $56.6\;{\mu}g/ml$ against the above eight fungi and R. solani was the most sensitive.

Studies on the constituents of the higher fungi of Korea (XI) An Antibiotic Component and a Sterol of Coriolus sanguineus Fr.

  • Chung, Kyong-Soo;Shim, Mi-Ja;Kim, Byong-Kak
    • Archives of Pharmacal Research
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    • v.1 no.1
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    • pp.33-40
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    • 1978
  • From the carpophores of Coriolus sanguineus Fr., a bright-red wood-rotting fungus, which were collected at Suwon, an antibiotic component was isolated as red crystal and identified as cinnabarin by UV and IR spectra. The antibiotic activities against five bacteria and three fungi were determined. It showed considerably high activity against Bacillus subtilis ATCC 6633 and Bacillus maceraus and also against all the three fungi tested. And from this fungus a sterol was isolated and identified as erogosterol by cochromatographing with four authentic sterols.

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On the Decay Rate of Soil Organic Matter and Changes of Soil Microbial populaiton (토양유기물의 분해속도와 Microbial populaiton의 소장에 관한 연구)

  • 김춘민
    • Journal of Plant Biology
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    • v.10 no.1_2
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    • pp.21-30
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    • 1967
  • The aim of present study is to elucidate the relationship between decay rate of soil organic matter, and the change of soil microbial population under the oak and pine forest soils in Kwang-nung plantation stand. The results obtained are as follows: 1) The correlation coefficient between decay rate and the soil bacteria is 0.84 and fungi 0.93. 2) The distribution of soil microbial population is higher in both F horizon of the oak forest soil, and F and H horizon of the pine forest soil. However, the number of soil microorganisms decreases with the depth in each forest soil. 3) The population of soil microbes is related to moisture content, total nitrogen, available phosphorus, and exchangeable calcium, except organic carbon in fungi. 4) The soil organic matter has been mainly decomposed by fungi, and the size of its population are governed by the factors such as moisture content, organic carbon, total nitrogen, available phosphorus, and exchangeable calcium.

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Notes on Korean Higher Fungi (II) (한국고등균류기(韓國高等菌類記) (II))

  • Lee, Ji-Yul;Cho, Duck-Hyun
    • The Korean Journal of Mycology
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    • v.5 no.2
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    • pp.17-20
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    • 1977
  • Several fungi were collected at Mt. Sobaek, Andong and Euisung the period from 1974 to 1976. These fungi were identified and results are as followingj Lipophyllum fallax (Peck.) $K{\ddot{u}}hn$ et Romagn. Armillariella tabescens (Scop. ex Fr.) Sing., Crepidotus subphaerosporus (Lange) $K{\ddot{u}}hn$. et Romagn., Stereum hiugense Imaz., Coprinus radians (Desm.) Fr., Hygrophorus suzukaensis Hongo and Xylaria carpophila (Pers.) Fr. were listed newly by authors in Korea.

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Biological Pretreatment of Softwood Pinus densiflora by Three White Rot Fungi

  • Lee, Jae-Won;Gwak, Ki-Seob;Park, Jun-Yeong;Park, Mi-Jin;Choi, Don-Ha;Kwon, Mi;Choi, In-Gyu
    • Journal of Microbiology
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    • v.45 no.6
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    • pp.485-491
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    • 2007
  • The effects of biological pretreatment on the Japanese red pine Pinus densiflora, was evaluated after exposure to three white rot fungi Ceriporia lacerata, Stereum hirsutum, and Polyporus brumalis. Change in chemical composition, structural modification, and their susceptibility to enzymatic saccharification in the degraded wood were analyzed. Of the three white rot fungi tested, S. hirsutum selectively degraded the lignin of this sortwood rather than the holocellulose component. After eight weeks of pretreatment with S. hirsutum, total weight loss was 10.7%, while lignin loss was the highest at 14.52% among the tested samples. However, holocellulose loss was lower at 7.81 % compared to those of C. lacerata and P. brumalis. Extracelluar enzymes from S. hirsutum showed higher activity of ligninase and lower activity of cellulase than those from other white rot fungi. Thus, total weight loss and changes in chemical composition of the Japanese red pine was well correlated with the enzyme activities related with lignin- and cellulose degradation in these fungi. Based on the data obtained from analysis of physical characterization of degraded wood by X-ray Diffractometry (XRD) and pore size distribution, S. hirsutum was considered as an effective potential fungus for biological pretreatment. In particular, the increase of available pore size of over 120 nm in pretreated wood powder with S. hirsutum made enzymes accessible for further enzymatic saccharification. When Japanese red pine chips treated with S. hirsutum were enzymatically saccharified using commercial enzymes (Cellulclast 1.5 L and Novozyme 188), sugar yield was greatly increased (21.01 %) compared to non-pre treated control samples, indicating that white rot fungus S. hirsutum provides an effective process in increasing sugar yield from woody biomass.

Profile of airborne microorganisms distributed in general offices (일반 사무실 실내공기 내 부유미생물의 분포 양상)

  • Kim, Ki Youn;Roh, Young Man;Kim, Yoon Shin;Lee, Cheol Min;Sim, In Suk
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.18 no.1
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    • pp.11-19
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    • 2008
  • Mean levels of airborne bacteria, airborne fungi, temperature, relative humidity and carbon dioxide in total 69 general offices were $426({\pm}83)\;cfu/m^3$, $234({\pm}125)\;cfu/m^3$, $25.9({\pm}1.3)\;^{\circ}C$, $57.7({\pm}8.6)\;%$, $422({\pm}38)\;ppm$, respectively. The I/O ratio of airborne bacteria and fungi was over 1 and there was no significant difference among temperature, relative humidity and carbon dioxide in total 69 general offices. In construction period, a concentration of airborne bacteria and fungi was significantly highest in general offices constructed under one year and over three years since construction, respectively (p<0.05). The concentration of airborne fungi in general offices located at basement was significantly higher than those located at ground (p<0.05). No significant difference of airborne bacteria and fungi in general offices was found regardless of installation of HVAC system (p>0.05). The dominant bacterial genera identified in general offices was Staphylococcus, followed by Micrococcus, Bacillus, and Corynebacterium while usarium, Penicillium, Aspergillus, Alternaria, Rhizopus and Mucor were identified as dominant fungal genera in general offices.

Screening and Evaluating of Wood-Rotting Fungi for Lignin Degradation and Ligninolytic Enzyme Production(I) - Screening of High Active Lignin-Degrading Fungi - (리그닌분해(分解)와 리그닌분해효소(分解酵素) 생산(生産)을 위한 목재부후균(木材腐朽菌)의 선발(選拔)과 평가(評價)(I) - 고활성(高活性) 리그닌분해균(分解菌)의 선발(選拔) -)

  • Jung, Hyun-Chae;Park, Seur-Kee;Kim, Byeong-Soo;Park, Chong-Yawl
    • Journal of the Korean Wood Science and Technology
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    • v.23 no.4
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    • pp.108-116
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    • 1995
  • This experiment was conducted to screen a superior wood-rotting fungi for lignin degradation and ligninolytic enzyme production by evaluation of red colored zone width on potato-dextrose agar medium and oak woodmeal medium complimented guaiacol. Relationship between the red colored zone width on GU-WA medium and klason lignin loss on woodmeal medium showed the positive correlation. Thus, the potential ligninolytic activity of wood rotting fungi which are not elucidated yet may be estimated to some extent by the evaluation of the red colored zone width on GU-WA medium. Of the isolates screened from fruit bodies and decayed woods. LKY-12, LKY-7 and C. versicolor-13 isolates having preferential lignin degradation and laccase activity were selected. These isolates exhibited characteristics of superior wood-rotting fungi as Klason lignin loss ranged from 30% to 35% and ligninolytic enzyme activity of these isolates on glucose-peptone broth was higher than that of other isolates. And then, these isolates were considered to be able to use in biological pulping and bleaching and ligninolytic enzyme production.

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Size-based Characteristics of Airborne Bacteria and Fungi Distributed in the General Hospital (종합병원의 실내공기에 분포하는 부유 세균과 진균의 입경별 종류와 특성)

  • Kim, Ki-Yeon;Lee, Chang-Rae;Kim, Chi-Nyon;Won, Jong Uk;No, Jaehoon
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.16 no.2
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    • pp.101-109
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    • 2006
  • The objective of this study is to provide fundamental data for pertinent management of indoor air quality through investigating the size-based characteristics of bioaerosol distributed in the general hospital. Measurement sites are main lobby, ICU, ward and laboratory and total five times were sampled with six-stage cascade impactor. Based on the result of this study, concentrations of airborne bacteria and fungi were the highest in main lobby as followed by an order of ward, ICU and laboratory. Concentrations of airborne bacteria was generally higher than those of airborne fungi and the ratio of indoor and outdoor concentration of both exceeded 1.0 in all the measurement sites of the general hospital. The predominant genera of airborne bacteria identified in the general hospital were Staphylococcus spp.(50%), Micrococcus spp.(15-20%), Corynebacterium spp.(5-20%), and Bacillus spp.(5-15%). On the other hand, the predominant genera of airborne fungi identified in the general hospital were Cladosporium spp.(30%), Penicillium spp.(20-25%), Aspergillus spp.(15-20%), and Alternaria spp.(10-20%). In regard to size distribution of bioaerosol, the detection rate was generally highest on 5 stage($1.1-2.1{\mu}m$) for airborne bacteria and on 1 stage(>$7.0{\mu}m$) for airborne fungi. Cleanliness of facilities in the general hospital and condition of HVAC system should be monitored regularly to prevent indoor air contamination by airborne microorganisms.