• Journal of Microbiology and Biotechnology
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• v.11 no.5
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• pp.890-894
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• 2001

The isolation of genomic DNA from mammalian cells is usually performed by cell lysis followed by protein digestion, extraction, and finally, ethanol precipitation of the chromosomal DNA. However, in the case of large sample numbers or when only small amounts of starting materials are available, such conventional methods are not efficient and are cumbersome to be applied. Some alternative methods have been described as well as having commercial DNA isolation kits to be available, nevertheless, there is room left for much improvement. In the present study, a novel method is introduced, where it simplifies conventional protocols by omitting some time-consuming steps such as protease incubation or DNA precipitation and its resuspension. Using paramagnetic silica resins, the genomic DNA was purified over a magnetic field, and the bound DNA was eluted with a low-salt buffer. The fidelity and effectiveness of this novel method was determined by using normal and apoptotic cells as a starting material and then compared to other protocols. The high speed and convenience along with its high efficiency in detecting apoptotic chromosomal DNA will prove this method to be an improved alternative in the isolation of genomic DNA from mammalian cells.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.2
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• pp.71-76
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• 2006

With the rapid development of spatial infrastructure in US, Europe, Japan, China and India, there is no doubt that the next generation Global Navigation Satellite System (GNSS) will improve the integrity, accuracy, reliability and availability of the position solution. GNSS is becoming an essential element of personal, commercial and public infrastructure and consequently part of our daily lives. However, the applicability of GPS in supporting a range of location-sensitive applications such as location based services in an urban environment is severely curtailed by the interference of the 3D urban settings. To characterize and gain in-depth understanding of such interferences and to be able to provide location-based optimization alternatives, a high-fidelity 3D urban model of Melbourne CBD built with ArcGIS and large scale high-resolution spatial data sets is used in this study to support a comprehensive simulation of current and future GNSS signal performance, in terms of signal continuity, availability, strength, geometry, positioning accuracy and reliability based on a number of scenarios. The design, structure and major components of the simulator are outlined. Useful time-stamped spatial patterns of the signal performance over the experimental urban area have been revealed which are valuable for supporting location based services applications, such as emergency responses, the optimization of wireless communication infrastructures and vehicle navigation services.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.10a
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• pp.100-104
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• 2005

The injection molding is very effective process for various plastic products due to its high productivity. It is also good fur precise products like optical parts. Various thermoplastic materials are also available with this injection molding process. In recent, however, as the overall size of the product increases and micro or nano scale of patterns are applied to the products, we now have some problems such as low fidelity of the replication of the pattern, high molding pressure, or warpage from the in-mold stress. Injection/compression molding is studied to overcome those problems in molding large thin plate with micro pattern array on its surface. An injection compression mold is designed to 3 pieces mold for side gate. We install 4 pressure transducers and 9 thermocouples to measure the melt pressure and surface temperature in the cavity during the process. As a result, the maximum molding pressure for injection compression molding is reduced to 1/3 compared to injection molding and the uniformity of the pressure in the cavity is enhanced by about 15%.

• Journal of the Korea Institute of Military Science and Technology
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• v.22 no.4
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• pp.460-466
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• 2019

It takes a lot of time and human resources to build a detailed three-dimensional finite element analysis model that is almost similar to the actual structure for sophisticated analysis, and a lot of experience and know-how is required to form a reliable analytical model. In this paper, the one-dimensional beam model connected by stiffness matrix through blade analysis library was compared with the results of three-dimensional analysis with the stress calculated through the process of dimensional restoration analysis based on the principle of virtual work. By comparing the stress calculated through dimensional restoration analysis with the three-dimensional analytical model, We will introduce the development status and application case of the blade analysis library by comparing efficiency and accuracy.

• Journal of the Korea Institute of Military Science and Technology
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• v.22 no.1
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• pp.49-59
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• 2019

Generative adversarial networks(GANs) have received great attention in the machine learning field for their capacity to model high-dimensional and complex data distribution implicitly and generate new data samples from the model distribution. This paper investigates the model training methodology, architecture, and various applications of generative adversarial networks. Experimental evaluation is also conducted for generating synthetic image dataset for defense using two types of GANs. The first one is for military image generation utilizing the deep convolutional generative adversarial networks(DCGAN). The other is for visible-to-infrared image translation utilizing the cycle-consistent generative adversarial networks(CycleGAN). Each model can yield a great diversity of high-fidelity synthetic images compared to training ones. This result opens up the possibility of using inexpensive synthetic images for training neural networks while avoiding the enormous expense of collecting large amounts of hand-annotated real dataset.

• Journal of the Semiconductor & Display Technology
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• v.16 no.3
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• pp.82-86
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• 2017

High dynamic range (HDR) imaging offers a radically approach of representing colors in digital images. Instead of using the range of colors produced by given devices, HDR imaging method manipulates and stores all colors and brightness levels visible to the human eye. To faithfully represent, store and then reproduce all these effects, the original scene must be stored and treated using high fidelity HDR techniques. Then, tone mapping is required to accommodate HDR image to low dynamic range (LDR) devices, and tone mapping operation of HDR image for realistic display is commonly researched. However, color visualization for analyzing scene luminance in HDR imaging has less attention from researches. This paper presents and implements a method for reproduction and visualization of the false color in HDR images. We produce a color visualization framework with several mapping functions, and evaluate their effectiveness by using RMAE and SNR with commonly used HDR image data. Experiment reveals that the sigmodal mapping function shows better performance in the false color visualization, compared to other methods.

• Structural Engineering and Mechanics
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• v.69 no.1
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• pp.21-31
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• 2019

Optimizing composite structures to exploit their maximum potential is a realistic application with promising returns. In this research, simultaneous maximization of the fundamental frequency and frequency separation between the first two modes by optimizing the fiber angles is considered. A high-fidelity design optimization methodology is developed by combining the high-accuracy of finite element method with iterative improvement capability of metaheuristic algorithms. Three powerful nature-inspired optimization algorithms viz. a genetic algorithm (GA), a particle swarm optimization (PSO) variant and a cuckoo search (CS) variant are used. Advanced memetic features are incorporated in the PSO and CS to form their respective variants-RPSOLC (repulsive particle swarm optimization with local search and chaotic perturbation) and CHP (co-evolutionary host-parasite). A comprehensive set of benchmark solutions on several new problems are reported. Statistical tests and comprehensive assessment of the predicted results show CHP comprehensively outperforms RPSOLC and GA, while RPSOLC has a little superiority over GA. Extensive simulations show that the on repeated trials of the same experiment, CHP has very low variability. About 50% fewer variations are seen in RPSOLC as compared to GA on repeated trials.

• Wind and Structures
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• v.28 no.4
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• pp.225-238
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• 2019

In this paper a novel and efficient computational framework to estimate the stress range versus number of cycles curves experienced by a cable due to external excitations (e.g., seismic excitations, traffic and wind-induced vibrations, among others) is proposed. This study is limited to the wind-cable interaction governed by the Vortex Shedding mechanism which mainly rules cables vibrations at low amplitudes that may lead to their failure due to bending fatigue damage. The algorithm relies on a stochastic approach to account for the uncertainties in the cable properties, initial conditions, damping, and wind excitation which are the variables that govern the wind-induced vibration phenomena in cables. These uncertainties are propagated adopting Monte Carlo simulations and the concept of importance sampling, which is used to reduce significantly the computational costs when new scenarios with different probabilistic models for the uncertainties are evaluated. A high fidelity cable model is also proposed, capturing the effect of its internal wires distribution and helix angles on the cables stress. Simulation results on a 15 mm diameter high-strength steel strand reveal that not accounting for the initial conditions uncertainties or using a coarse wind speed discretization lead to an underestimation of the stress range experienced by the cable. In addition, parametric studies illustrate the computational efficiency of the algorithm at estimating new scenarios with new probabilistic models, running 3000 times faster than the base case.

• International Journal of Computer Science & Network Security
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• v.22 no.3
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• pp.382-388
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• 2022

Satellite communication for high altitude platform stations (HAPS) and low earth orbit (LEO) systems suffer from dust and sand (DU&SA) storms in the desert regions such as Saudi Arabia. These attenuations have a distorting effect on signal fidelity at high frequency of operations. This results signal to noise ratio (SNR) to dramatically decreasing and leads to wireless transmission error. The main focus in this paper is to propose common relations between HAPS and LEO for the atmospheric impairments affecting the satellite communication networks operating above Ku-band crossing the propagation path. A double phase three dimensional relationship for HAPS and LEO systems is then presented. The comparison model present the analysis of atmospheric attenuation with specific focus on sand and dust based on particular size, visibility, adding gaseous effects for different frequency, and propagation angle to provide system operations with a predicted vision of satellite parameters' values. Skillful decision and control system (SD&CS) is proposed to control applied parameters that lead to improve satellite network performance and to get the ultimate receiving wireless signal under bad weather condition.

• Nuclear Engineering and Technology
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• v.55 no.9
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• pp.3388-3400
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• 2023

Detailed analysis of the neutron pathway through matter inside the nuclear reactor core is exceedingly needed for safety and economic considerations. Due to the constant development of high-performance computing technologies, neutronics analysis using computer codes became more effective and efficient to perform sophisticated neutronics calculations. In this work, a commercial pressurized water reactor (PWR) presented by Virtual Environment for Reactor Applications (VERA) Core Physics Benchmark are modeled and simulated using a high-fidelity simulation of OpenMC code in terms of criticality and fuel pin power distribution. Various problems have been selected from VERA benchmark ranging from a simple two-dimension (2D) pin cell problem to a complex three dimension (3D) full core problem. The development of the code capabilities for reactor physics methods has been implemented to investigate the accuracy and performance of the OpenMC code against VERA SCALE codes. The results of OpenMC code exhibit excellent agreement with VERA results with maximum Root Mean Square Error (RMSE) values of less than 0.04% and 1.3% for the criticality eigenvalues and pin power distributions, respectively. This demonstrates the successful utilization of the OpenMC code as a simulation tool for a whole core analysis. Further works are undergoing on the accuracy of OpenMC simulations for the impact of different fuel types and burnup levels and the analysis of the transient behavior and coupled thermal hydraulic feedback.