• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.4
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• pp.274-284
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• 2022

To use kenaf (Hibiscus cannabinus L.) as a fiber and livestock feed, a high-yielding variety needs to be identified. For this, accurate phenotyping of plant height is required for this breeding purpose due to the strong relationship between plant height and yield. Plant height can be estimated using RGB images from unmanned aerial vehicles (UAV-RGB) and photogrammetry based on Structure from Motion (SfM) algorithms. In kenaf, accurate measurement of height is limited because kenaf stems have high flexibility and its height is easily affected by wind, growing up to 3 ~ 4 m. Therefore, we aimed to identify a method suitable for the accurate estimation of plant height of kenaf and investigate the feasibility of using the UAV-RGB-derived plant height map. Height estimation derived from UAV-RGB was improved using multi-point calibration against the five different wooden structures with known heights (30, 60, 90, 120, and 150 cm). Using the proposed method, we analyzed the variation in temporal height of 23 kenaf cultivars. Our results demontrated that the actual and estimated heights were reliably comparable with the coefficient of determination (R²) of 0.80 and a slope of 0.94. This method enabled the effective identification of cultivars with significantly different heights at each growth stages.

• Korean Journal of Optics and Photonics
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• v.32 no.5
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• pp.215-219
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• 2021

Recently a system for nondestructive measurement of seedling plants in real time has been attracting attention as an essential element in fields such as the "smart farm". This study reports the simultaneous measurement of anthocyanin accumulations in leaf tissues in a large number of bok choy, using a hyperspectral imaging system. To measure many seedlings simultaneously, an existing hyperspectral imaging system is modified. In this paper, a total of 96 seedlings are measured: 24 each of 4 cultivars. Using the hyperspectral data-acquisition system, 12 seedlings can be analyzed simultaneously within 3 minutes. The hyperspectral imaging technology proposed in this paper is shown to provide an analytic system comparable to destructive chemical analysis. This hyperspectral imaging technology can be applied to a high-throughput plant-phenotyping system, owing to its capability of measuring a large number of specimens at the same time.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.25-25
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• 2022

Drought becomes frequent and severe because of continuous global warming, leading to a significant loss of crop yield. In soybean (Glycine max [L.]), most of quantitative trait loci (QTLs) analyses for drought tolerance have conducted by investigating yield changes under water-restricted conditions at the reproductive stages. More recently, the necessity of QTL studies to use physiological indices responding to drought at the early growth stages besides the reproductive ones has arisen due to the unpredictable and prevalent occurrence of drought throughout the soybean growing season. In this study, we thus identified QTLs conferring wilting scores and moisture contents of soybean subjected to drought stress in the early vegetative stage using an recombinant inbred line (RIL) population derived from a cross between Taekwang (drought-sensitive) and SS2-2 (drought-tolerant). For the two traits, the same major QTL was located on chromosome 10, accounting for up to 11.5% of phenotypic variance explained with LOD score of 12.5. This QTL overlaps with a reported QTL for the limited transpiration trait in soybean and harbors an ortholog of the Arabidopsis ABA and drought-induced RING-D UF1117 gene. Meanwhile, one of important features of plant drought tolerance is their ability to limit transpiration rates under high vapor pressure deficiency in response to mitigate water loss. However, monitoring their transpiration rates is time-consuming and laborious. Therefore, only a few population-level studies regarding transpiration rates under the drought condition have been reported so far. Via employing an Arduino-based platform, for the reasons addressed, we are measuring and recording total pot weights of soybean plants every hour from the 1^st day after water restriction to the days when the half of the RILs exhibited permanent tissue damage in at least one trifoliate. Gradual decrease in moisture of soil in pots as time passes refers increase in the severity of drought stress. By tracking changes in the total pot weights of soybean plants, we will infer transpiration rates of the mapping parents and their RILs according to different levels of VPD and drought stress. The profile of transpiration rates from different levels of severity in the stresses facilitates a better understanding of relationship between transpiration-related features, such as limited maximum transpiration rates, to water saving performances, as well as those to other drought-responsive phenotypes. Our findings will provide primary insights on drought tolerance mechanisms in soybean and useful resources for improvement of soybean varieties tolerant to drought stress.