• Radiation Oncology Journal
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• v.17 no.2
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• pp.151-157
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• 1999

Purpose : By sequencing the Erpressed Sequence Tags of human 걸ermal papilla CDNA library, we identified a clone named K872 of which the expression decreased during differentiation of HL6O cell line. Materials and Methods : K872 plasmid DNA was isolated according to QIA plasmid extraction kit (Qiagen GmbH, Germany). The nucleotide sequencing was performed by Sanger's method with K872 plasmid DNA. The most updated GenBank EMBL necleic acid banks were searched through the internet by using BLAST (Basic Local Alignment Search Tools) program. Nothern bots were performed using RNA isolated from various human tissues and cancer cell lines. The gene expression of the fusion protein was achieved by His-Patch Thiofusicn expression system and the protein product was identified on SDS-PAGE. Results : K872 clone is 1006 nucleotides long, and has a coding region of 675 nucleotides and a 3' non-coding region of 280 nucleotides. The presumed open reading frame starting at the 5' terminus of K872 encodes 226 amino acids, including the initiation methionine residue. The amino acid sequence deduced from the open reading frame of K872 shares $70\%$, identity with that of rat glutathione 5-transferase kappa 1 (rGSTKl). The transcripts were expressed in a variety of human tissues and cancer cells. The levels of transcript were relatively high in those tissues such as heart, skeletal muscle, and peripheral blood leukocyte. It is noteworthy that K872 was found to be abundantly expressed in coloreetal cancer and melanoma cell lines. Conclusion : Homology search result suggests that K872 clone is the human homolog of the rGSTK1 which is known to be involved in the resistance of cytotoxic therapy. We propose that meticulous functional analysis should be followed to confirm that.

• Korean Journal of Food Science and Technology
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• v.39 no.6
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• pp.681-687
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• 2007

This study was designed to examine the suitable characteristics of potential probiotic bacteria. Possible probiotic bacteria, including Lactobacillus acidophilus DDS-1, Lb. acidophilus B-3208, Bifidobacterium bifidum KCTC 3357, Lb. plantarum, Leuconostoc mesenteroides ssp. mesenteroides ATCC 8293, and Lactococcus lactis ssp. lactis ATCC 7962 were selected. We then measured their acid and bile tolerances, adhesion properties in the gastrointestinal tract, antimicrobial activity against pathogenic bacteria, and immunomodulation activity. The acid tolerances of Lb. acidophilus DDS-1, Lb. acidophilus B-3208, Lb. plantarum, and Leu. mesenteroides ssp. mesenteroides ATCC 8293, in PBS (pH 2.5) for 2 hr, were high enough that 50% of the inocula survived. The bile tolerances of all bacteria, except Lc. lactis ssp. lactis ATCC 7962, were also observed at a 3% oxgall concentration in MRS broth. The results of the adhesion property assay showed that the total binding affinities of Lb. acidophilus DDS-1, Lb. acidophilus B-3208, and B. bifidum were about three times higher than those of the other bacteria. In testing their antimicrobial activities against pathogens, Lb. acidophilus B-3208, B. bifidum KCTC 3357, and Lb. plantarum inhibited the growth of pathogenic bacteria. For their immunomodulation activity, the cell wall fractions from Lb. acidophilus DDS-1 and Lb. acidophilus B-3208 showed the highest bone marrow cell proliferation activities. However, the cell wall fractions of Lb. acidophilus DDS-1 and B. bifidum, and the cytosol fraction of Lc. lactis ssp. lactis ATCC 7962 showed higher macrophage stimulation activities than those of the other bacteria. Since Lb. acidophilus DDS-1 and Lb. acidophilus B-3208 satisfy the requirements for probiotics, they can be considered suitable probiotic bacteria.

• Food Science and Preservation
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• v.22 no.3
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• pp.399-407
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• 2015

Sumaeyaksuk (Artemisia Argyi H.) is one of the original mugwort spices in Namhae-gun, Korea. This study was conducted to investigate the physicochemical characteristics and biological activities of water extracts from dried and aging Sumaeyaksuk samples during the May-July harvest season. One (SD) was dried under shade for 12 days, while the other (AD) was aged for 7 days at $60^{\circ}C$ and then roasted for 220 minutes at over $90^{\circ}C$. Glucose was solely detected as a free sugar, and its SD and AD content were $0.42{\pm}0.02{\sim}0.43{\pm}0.01g/100g$, and $0.41{\pm}0.02{\sim}0.47{\pm}0.04g/100g$, respectively. The total phenolic contents of SD were $1.85{\pm}0.09{\sim}3.45{\pm}0.14g/100g$, which were higher than those of AD ($1.29{\pm}0.08{\sim}2.90{\pm}0.08g/100g$). The antioxidant activities of the water extract powder from each Sumaeyaksuk were assessed by different in vitro methods, such as the DPPH and ABTS radical scavenging activity, FRAP, and decoloration prevention activity in ${\beta}$-carotene linoleic system. The DPPH and ABTS radical scavenging activity of AD extract were significantly higher than those of the SD extract (p<0.05). Moreover, at the concentrations of 31.25, 62.5, 125, 250, $500{\mu}g/mL$, the FRAP of the SD-May extract showed $1.67{\pm}0.58{\sim}489.90{\pm}7.59{\mu}M$, while the AD-July extract showed $9.70{\pm}1.07{\sim}590.40{\pm}7.45{\mu}M$. The ${\beta}$-carotene decoloration prevention activity of the SD-May and AD-July extracts were $25.53{\pm}2.85{\sim}81.43{\pm}2.56%$, $35.98{\pm}2.22{\sim}79.00{\pm}1.42%$, respectively. Based on these results, the extracts of SD-May and AD-July were promising as a functional food source due to their high antioxidant activites.

• Journal of Technologic Dentistry
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• v.19 no.1
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• pp.21-35
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• 1997

The Ag-Pd-Cu alloys containing a small amount of Au is commonly used for dental purposes, because this alloy is cheaper than Au-base alloys for clinical use. However, the most important characteristic of this alloy is age-hardenability, which is not exhibited by other Ag-base dental alloys. The specimens used were Ag-20Pd-20Cu ternary alloy and Au addition alloy. These alloys were melted and casted by induction electic furace and centrifugal casting machine in Ar atmoshpere. These specimens were solution treated for 2hr at $800^{\circ}C$ and were then quenched into iced water, and aged at $350{\sim}550^{\circ}C$ Age-hardening characteristics of the small Au-containing Ag-pPd-Cu dental alloys were investigated by means of hardness testing, X-ray diffraction and electron microscope observations, electrical resistance, differential scanning calorimetric, emergy dispersed spectra and electron probe microanalysis. Principal results are as follows : Hardening occured in two stages, I. e., stage I in low temperature and stage II in high temperature regions, during continuous aging. The case of hardening in stage I was due to the formation of the Llo type face centered tetragonal PdCu-ordered phase in the grain interior and hardening in stage I was affedted by the Cu concentration. In stage II, decomposition of the $\alpha$ solid solution to a PdCu ordered phase(L1o type) and an Agrich ${\alpha}2$ phase occurred and a discontiunous precipitation occurred at the grain boundary. Form the electron microscope study, it was concluded that the cause of age-hardening in this alloy is the precipitation of the PdCu ordered phase, which has AuCu I type face-centered tetragonal structure. Precipitation procedure was ${\alpha}\to{\alpha}+{\alpha}2+PdCu\to{\alpha}1+{\alpha}2+PdCu$ at Pd/Cu = 1 Ag-Pd-Cu alloy is more effective dental alloy as ageing treatment and is suitable to isothermal ageing at $450^{\circ}C$.

• Journal of the Korean Electrochemical Society
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• v.9 no.3
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• pp.124-131
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• 2006

For commercialization of molten carbonate fuel cell (MCFC), it has some problems to be overcome such as decrease of porosity and thickness of the anode under the operating condition (at $650^{\circ}C$ and working pressure of more than 2 $kg_f/cm^2$). Recently, Ni-Al alloy anode has been proposed to replace the conventional Ni-Cr anode as an alternative material to resist a creep and inhibit the sintering. The objective of this research is to sinter the green sheet of Ni-Al alloy anode during single cell pre-treatment process, which has several advantages like cost down and simplification of manufacturing process. However, the Ni-Al alloy anode prepared with a conventional pre-treatment process showed the phase separation of Ni-Al alloy and formation of micropore(${\leqq}0.4{\mu}m$), resulting in low creep resistance and high electrolyte re-distribution. In order to prevent the Ni-Al alloy anode from phase-separating, nitrogen gas was used in the process of pre-treatment. Introducing the nitrogen, the phase separation from Ni-Al alloy into nickel and alumina was minimized and increased creep resistance. However, there was some micropore formation on the surface of Ni-Al alloy anode during the cell operation due to creation of lithium aluminate. Addition of more amount of electrolyte into a cell, especially at cathode, made the cell performance stable for 2,000 hrs. Consequently, it was possible to make the Ni-Al alloy anode with good creep resistance by the modified in-situ sintering technique.

• Clean Technology
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• v.25 no.3
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• pp.245-255
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• 2019

Pressurized oxy-fuel combustion is a promising technology for $CO_2$ capture with a benefit of improving power plant efficiency compared with atmospheric oxy-fuel combustion. Prior to $CO_2$ compression in this process, a flue gas condenser (FGC) is used to remove $H_2O$ while recovering the latent heat. At the same time, the FGC has a potential for high-efficiency removal of $SO_x$ and $NO_x$ by exploiting their good solubility in water. In this study, experiments were carried out in a lab-scale, direct contact FGC under different pressures varying between 1 and 20 bar to evaluate the removal efficiency of $SO_2$ and $NO_x$ for individual gases and their mixture. In the tests for individual gases, 20% and 76% of $NO_x$ was removed at 1 bar and 10 bar, respectively. Even higher removal efficiencies were achieved for $SO_2$, and also these were maintained for longer as the pressure increased. In the tests for $SO_2$ and $NO_x$ mixture, the removal efficiency of $NO_x$ increased from 13% at 1 bar to 56% at 20 bar because of higher solubility at elevated pressures. $SO_2$ in the mixture was initially dissolved almost completely and then increased by 1,219 ppm at 1 bar and by 165 ppm at 20 bar. Overall, the removal efficiency of $SO_2$ and $NO_x$ was increased at elevated pressures, but it was lower in the mixture compared with individual gases at identical conditions because of a lower pH and associated chemical reactions in water.

• Journal of Practical Agriculture & Fisheries Research
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• v.25 no.4
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• pp.24-38
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• 2024

Five species of plants (Pimpinella brachycarpa (Kom.) Nakai, Hylotelephium erythrostictum (Miq.) H. Ohba, Magnolia sieboldii K. Koch, Sanguisorba hakusanensis Makino, Agastache rugosa (Fisch. & Mey.) Kuntze) native to the clean area of Mt. Jiri were selected. The collection period was from May to September 2021, and the five species plants were collected in their native habitats with flowers in full bloom. The collected plants were extracted with 70% EtOH and 17 kinds of polyphenol components were analyzed. Next, flowers, leaves and roots were separated from plants, extracted with 70% EtOH for each part and experiments were conducted on DPPH, ABTS, total polyphenols, and total flavonoids. The results are as follows. 1. There were a total of 13 polyphenol components contained in the 5 species of plants native to Mt. Jiri. Among them, the total content of the most detected substance was Sanguisorba hakusanensis Makino, which was 126.2 ppm, and the main substance was ellagic acid (=ELA), which was 122.4 ppm. 2. As a result of examining the DPPH radical scavenging activity, the flowers, stems, and roots of Sanguisorba hakusanensis Makino showed the best scavenging activity, while the flower extracts of other plants showed good scavenging activity. 3. The highest ABTS radical scavenging activity was Sanguisorba hakusanensis Makino flower extract at 13.5 ㎍/㎖, followed by roots at 16.8 ㎍/㎖ and leaves at 22.6 ㎍/㎖;. Although such a large ABTS radical scavenging activity could not be confirmed in other plants, the flower extract was overall better than the leaf and root extracts of other parts. 4. The highest total polyphenol content was found in the leaves of Magnolia sieboldii K. Koch, at 161.4mg GAE/g, followed by the highest content in the roots of Magnolia sieboldii K. Koch and the roots of Agastache rugosa (Fisch. & Mey.) Kuntze, at 130.0mg GAE/g. Also, the lowest was found to be Sanguisorba hakusanensis Makino. 5. The total flavonoid content was 186.2mg CAE/g in the roots of Agastache rugosa (Fisch. & Mey.) Kuntze, 166.9mg CAE/g in the flowers, and the lowest was 116.1mg CAE/g in the leaves. As shown in the results above, Sanguisorba hakusanensis Makino has excellent antioxidant efficacy and has a high polyphenol content, so it is expected to be fully utilized in the cosmetics industry in the future.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.3
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• pp.385-392
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• 2012

This study was conducted to find out the optimum cropping system for the stable production of forage crops in the newly reclaimed land located at Gwanghwal and Gyehwa region of Saemangum reclaimed tide land from October, 2009 to October, 2011. Whole crop barley (WCB), Rye, Italyan-ryegrass (IRG) as winter crops and Corn, Sorghum${\times}$sudangrass hybrid (SSH) as summer crops were cultivated. Soil chemical properties, nutrient uptake, feed value, growth and yield were examinated. The testing soil was showed saline alkali soil where the contents of organic matter, available phosphate and exchangeable calcium were very low, while exchangeable sodium and magnesium were higher. Changes of soil salinity during the growing season of forage crops were less than 0.2%, and the growth of forage crops was not affected by salt injury. Standing rates of winter crops were higher in the order of Rye, WCB, and IRG, while the dry matter yield of winter crops was higher in the order of IRG, Rye and WCB. The highest crude protein (CP) content was recorded in IRG, and total digestive nutrients (TDN) contents were increased in the order of WCB, IRG, and Rye. The TDN content was higher in corn, whereas other feed value was higher in SSH. The content of mineral nutrients on stem, leaf and grain in IRG, Corn were high. After experiment pH was lowed, contents of exchangeable magnesium, sodium and organic matter were decreased while contents of total nitrogen, available phosphate and exchangeable potassium, calcium were increased. Winer crops and summer crops after continually cultivating in cropping system, fresh matter yield increased, compared to WCB-Corn (74,740 kg $ha^{-1}$), IRG-SSH 10%, IRG-Corn 7%, Rye-SSH 6%, Rye-Corn and WCB-SSH 3%. Dry matter yield increased, compared to WCB-Corn (20,280 kg $ha^{-1}$), IRG-SSH 7%, Rye-SSH 6%, IRG-Corn/Rye-Corn/WCB-SSH 3%. The TDN yield increased, compared to WCB-Corn (13,830 kg $ha^{-1}$), IRG-SSH 2%, WCB-SSH and IRG-Corn 1%. Therefore, we suggest that the crop combination of IRG-SSH and WCB-SSH would be preferred for silage stable production.

• Korean Journal of Environment and Ecology
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• v.31 no.6
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• pp.537-548
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• 2017

The purpose of this study was to investigate the species composition and the aquatic environment of Jojong Stream and Sudong Stream, which were the original habitats of Zacco koreanus population and restored population re-introduced in Bongseonsa Stream. It also compared and analyzed the states of the growth and reproductive ability of Z. koreanus habiting in each of the three streams. The investigation was conducted in June 2016 which was known as the spawning season of Z. koreanus. The results of the physical aquatic environments showed the slight differences in altitude, width and depth of water among three streams, but the bottom structure was found to be quite different in the composition of the boulder, cobble, and pebble among the streams. The result of the physicochemical aquatic environment analysis showed that there were no significant differences in water temperature, pH, DO, BOD, and EC among the three stream. In the fish fauna investigation, 530 individuals of 11 species of 3 families were collected in Bongseonsa Stream, 293 individuals of 12 species of 4 families were collected in Jojong Stream, and 361 individuals of 11 species of 4 families were collected in Sudong Stream. All three streams were dominated by Z. koreanus and Z. platypus. Six Korean endemic species appeared in each of the three streams, showing the high occurrence rate of indigenous species of 50.0% or more. The aggregation index analysis revealed that the mean dominance index ranged from 0.63 (${\pm}0.05$, BS) to 0.72(${\pm}0.01$, JJ), mean diversity index from 1.55 (${\pm}0.06$, JJ) to 1.78 (${\pm}0.11$, BS), mean evenness index from 0.71 (${\pm}0.03$, JJ) to 0.76 (${\pm}0.02$, BS), and mean richness index from 1.61 (${\pm}0.33$, JJ) to 1.73 (${\pm}0.24$, SD). The result indicated that the observed differences between the stream community indices were statistically nonsignificant. The similarity analysis showed that 75.4% similarity was divided into two groups of A and B and that the fish fauna on each analyzed point was similar. The quantitative habitat evaluation index (QHEI) analysis showed that the average value of QHEI was 151.0 (${\pm}46.0$), which means that it was a suboptimal habitat environment. The result of length-weight analysis of Z. koreanus populations showed that the regression coefficient b of the restoration population and the original habitat population were at 3.0 or higher while the condition factor had a positive slope. Moreover, it was found that the slopes of the regression coefficient b and condition factor of the original habitat population were larger than the restored population. The analysis of the length frequency distribution of the Z. koreanus population revealed that all three streams maintained the stable life cycle although it was found that the growth rate of the original habitat population was faster than the restored population in the one-year-old class. The result of the gonadosomatic index (GSI) analysis showed that the GSI median value of the Z. koreanus population in the restored habitat Bongseonsa Stream was higher than the population in the original habitat Jojong Stream and Sudong Stream for both of males and females.

• Economic and Environmental Geology
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• v.42 no.3
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• pp.177-193
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• 2009

The Samgwang deposit consists of eight massive mesothermal quartz veins that filled NE and NW-striking fractures along fault zones in Precambrian granitic gneiss of the Gyeonggi massif. The mineralogy and paragenesis of the veins allow two separate discrete mineralization episodes(stage I=quartz and calcite stage, stage II-calcite stage) to be recognized, temporally separated by a major faulting event. The ore minerals are contained within quartz and calcite associated with fracturing and healing of veins that occurred during both mineralization episodes. The hydrothermal alteration of stage I is sericitization, chloritization, carbonitization, pyritization, silicification and argillization. Sericitic zone occurs near and at quartz vein and include mainly sericite, quartz, and minor illite, carbonates and chlorite. Chloritic zone occurs far from quartz vein and is composed of mainly chlorite, quartz and minor sericite, carbonates and epidote. Fe/(Fe+Mg) ratios of sericite and chlorite range 0.45 to 0.50(0.48$\pm$0.02) and 0.74 to 0.81(0.77$\pm$0.03), and belong to muscovite-petzite series and brunsvigite, respectiveIy. Calculated $Al_{IV}$-FE/(FE+Mg) diagrams of sericite and chlorite suggest that this can be a reliable indicator of alteration temperature in Au-Ag deposits. Calculated activities of chlorite end member are $a3(Fe_5Al_2Si_3O_{10}(OH)_6$=0.0275${\sim}$0.0413, $a2(Mg_5Al_2Si_3O_{10}(OH)_6$=1.18E-10${\sim}$7.79E-7, $a1(Mg_6Si_4O_{10}(OH)_6$=4.92E-10${\sim}$9.29E-7. It suggest that chlorite from the Samgwang deposit is iron-rich chlorite formed due to decreasing temperature from high temperature(T>450$^{\circ}C$). Calculated ${\alpha}Na^+$, ${\alpha}K^+$, ${\alpha}Ca^{2+}$, ${\alpha}Mg^{2+}$ and pH values during wallrock alteration are 0.0476($400^{\circ}C$), 0.0863($350^{\circ}C$), 0.0154($400^{\circ}C$), 0.0231($350^{\circ}C$), 2.42E-11($400^{\circ}C$), 7.07E-10($350^{\circ}C$), 1.59E-12($400^{\circ}C$), 1.77E-11($350^{\circ}C$), 5.4${\sim}$6.4($400^{\circ}C$), 5.3${\sim}$5.7($350^{\circ}C$)respectively. Gain elements(enrichment elements) during wallrock alteration are $TiO_2$, $Fe_2O_3(T)$,CaO, MnO, MgO, As, Ag, Cu, Zn, Ni, Co, W, V, Br, Cs, Rb, Sc, Bi, Nb, Sb, Se, Sn and Lu. Elements(Ag, As, Zn, Sc, Sb, Rb, S, $CO_2$) represents a potential tools for exploration in mesothermal and epithermal gold-silver deposits.