• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.6
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• pp.349-353
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• 2003

The physiological characteristics of cultures of very high cell mass (e.g. 10g cell mass/L), termed“ultrahigh cell density cultures”is reviewed. A close relationship was found between the length of the optical path (OP) in flat-plate reactors and the optimal cell density of the culture as well as its areal (g m$\^$-2/ day$\^$-1/) productivity. Cell-growth inhibition (GI) unfolds as culture density surpasses a certain threshold. If it is constantly relieved, a 1.0cm OP reactor could produce ca. 50% more than reactors with longer OP, e.g. 5 or 10cm. This unique effect, discovered by Hu et al. [3], is explained in terms of the relationships between the frequency of the light-dark cycle (L-D cycle), cells undergo in their travel between the light and dark volumes in the reactor, and the turnover time of the photosynthetic center (PC). In long OP reactors (5cm and above) the L-D cycle time may be orders of magnitude longer than the PC turnover time, resulting in a light regime in which the cells are exposed along the L-D cycle, to long, wasteful dark periods. In contrast, in reactors with an OP of ca. 1.0 cm, the L-D cycle frequency approaches the PC turnover time resulting in a significant reduction of the wasteful dark exposure time, thereby inducing a surge in photosynthetic efficiency. Presently, the major difficulty in mass cultivation of ultrahigh-density culture (UHDC) concerns cell growth inhibition in the culture, the exact nature of which is awaiting detailed investigation.

• KSBB Journal
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• v.14 no.2
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• pp.160-166
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• 1999

High cell density cultivation of microalga Dunaliella bardawil using nitrogen fed-batch cultures was studied in batch flask. Optimum environmental conditions include concentrated nutrients except NaCl and carbon sources, carbon sources, pH, light, agitation, nitrate and phosphate ions. Cell growth, consumption rates of nitrate and phosphate ions were monitored. Optimal conditions for higher cell density were found to be(in the range tested): 5 times concentrated media(1 times-10 times concentrated media) pH 8.0 (7.0-9.0) white light(blue and red light) 15mM of nitrate (0.94-15mM) 250mM $NaHCO_3$ and $CO_2$ gas. However, the addition of phosphate ions did not enhance the algal maximum cell density and specific growth rate. Nitrate was found to be effective for the cell growth. The maximum cell density of fed-batch culture using nitrate ions in $8.955{\times}106$cells/ml after 189hr incubation.

• Korean Journal of Microbiology
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• v.21 no.1
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• pp.7-14
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• 1983

In order to study the effects of cropping system and fungicide (Dachigaren) on soil microbes, the seasonal fluctuations of soil microbes in the fields of radish and Chinese cabbage including soil pH, Soil moisture content and soil temperature were investigated on every 15 day interval from the begining of March to late October in 1981. The population of total fungus peaked at the begining of July, while that of total bacteria, at the begining of August. They were affected by soil temperature, however pathogenic microbes seemed to be more related with host plants than the soil temperature, because pathogens showed high density through the whole cultivation period. The pathogenic microbes showed the density of order ; Xanthomonas, Erwinia, Pseudomonas, Agrobacterium and Corynebacterium. Xanthomonas, Erwinia and Pseudomonas, which induced radish and Chinese cabbage diseases were higher than Agrobacterium and Corynebacterium in population densigy. Bacterial soft rot occured at the density of Erwinia $5.9{\sim}6.6{\times}10^5/dry$ soil 1 gram. The density of microbes on continuous fields were higher than that of rotating fields, but there were no significant difference between treated fungicide plot and non treated in the density of microbes, also no difference between Chinese cabbage and radish growing fields.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.3
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• pp.193-202
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• 2017

This study was carried out in 2014 and 2015 to investigate the effect of transplanting density on panicle production and to analyze the relationship of panicle production with yield traits in the mid-maturing quality rice cultivar 'Haiami' in the mid-plain area of Korea. Number of tillers per square meter increased by reducing planting distance and increasing seedling number per hill. These effects were maintained until maturity and were reflected in the final number of panicles per square meter, although the effect of planting density decreased as rice growth progressed. However, increased panicle number per square meter following dense planting did not improve head rice yield. Panicle number per square meter was negatively associated with spikelet number per panicle and was not correlated with other yield components or head rice yield. Head rice yield was not correlated with head rice percentage but was closely and positively correlated with milled rice yield. Milled rice yield did not increase with grain filling percentage but increased with spikelet number per square meter. Spikelet number per square meter increased with both spikelet number per panicle and panicle number per square meter, although the former had a greater influence. Therefore, we conclude that head rice yield of the 'Haiami' cultivar grown in the mid-plain area of Korea is not improved with an increased panicle number following high planting density but it could be improved with high milled rice yield by increasing spikelet number per unit area.

• KSBB Journal
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• v.5 no.3
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• pp.307-313
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• 1990

A microcomputer-aided fermentation system was constructed for high density fed-batch culture using dissolved oxygen(DO) as a substrate feeding indicator. DO signal was processed prior to aquisition to computer. Agitation speed and oxygen flow rate was changed stepwisely to maintain DO value at a constant level. Agitation speed was controlled by the output signal of D/A converter. Oxygen flow rate was controlled by a flow rate control valve connected to a stepping motor. Substrate was fed with a feeding pump operated by the abrupt increase of DO signal. Methylobacillus sp. SK1 was cultivated to test the system and 16.53g/l of cell density was obtained after 10 hr.

• Korean Journal of Medicinal Crop Science
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• v.14 no.3
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• pp.134-138
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• 2006

The result that test 3 years since 2002 allowing 6 processing such as $40{\times}5\;cm$ to supply basis data of method of cultivation establishment investigating productivity of Saururus chinensis by the number of cultivation years and planting distance, is as following. Plant height of foliar growth was good 1 year eats little, but 2 years planting distance $40{\times}10\;cm$, 3 years was best in $40{\times}20\;cm$ and there were many the number of tillerings 1, 2, all 3 years do high plant density, and rhizoma growth is 1, 2 years does high plant density, it was good tendency, but 3 years is best in $40{\times}20\;cm$. 1 year is most in planting distance $40{\times}5\;cm$, 2 years $40{\times}10\;cm$, 3 years $40{\times}20\;cm$, $1{\sim}2$ year accumulation quantity $40{\times}10\;cm$, $1{\sim}3$ year accumulation quantity $40{\times}10$, 20 cm in goods foliar amount.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.6
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• pp.606-613
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• 2003

This study investigated the effects of food type (condensed freshwater Chlorella, dried Chlorella, dried Spirulina, dried Schizochytrium, baker's yeast and $\omega-yeast$) and amount, and supplementation of vitamin $B_{12}$ on the growth of freshwater rotifer (Brachionus calyciflorus) in high density culture. Growth of rotifers fed condensed freshwater Chlorella was the highest and its density ranged $7.65-8.14{\times}10^3\;inds./mL.$ The primary lipid acids of rotifers fed condensed freshwater Chloyella were linoleic and linolenic, and their amount ($\%$ of total fatty acids) were $48.8\%\;and\;26.8\%,$ respectively. This suggests that condensed freshwater Chlorella would be an effective diet for high quality and quantity rotifers, which in turn serve as live food for freshwater fish larvae. Growth rate of rotifers with Chlorella supplementation increased as amount of supplementation increased up to 1.5 and 2.5 mg at 28 and $32^{\circ}C$, respectively. However, undissolved ammonia toxicity and packing volume of Chlorella in culture medium, reached the optimal conditions for the stable and effective cultivation of rotifers when amount of condensed freshwater Chlorella was 1.5 mg in dry weight per 1,000 rotifers at $28^{\circ}C\;and\;32^{\circ}C$ Growth of rotifers in condensed freshwater Chlorella with vitamin $B_{12}$ supplementation was significantly higher than that of rotifers without supplementation. However, no significant difference was found among the different concentrations of vitamin $B_{12}.$ Therefore, vitamin $B_{12}$ could improve the growth of rotifers (B. calyciflorus).

• Journal of Microbiology and Biotechnology
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• v.12 no.5
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• pp.753-759
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• 2002

$\beta$-Cyclodextrin glucanotransferase ($\beta$-CGTase) was overproduced extracellularly using recombinant E. coli by transforming the plasmid pECGT harboring a secretive signal peptide. The $\beta$-CGTase gene of alkalophilic Bacillus firmus var alkalophilus was inserted into the high expression vector pET20b(+) containing a secretive pelB signal peptide, and then transformed into E. coli BL2l(DE3)pLysS. The optimum culture conditions fer the overproduction of $\beta$-CGTase were determined to be TB medium containing 0.5% (w/v) soluble starch at post-induction temperature of $25^{\circ}C$. A significant amount of $\beta$-CGTase, up to 5.83 U/ml, which was nine times higher than that in the parent strain B. firmus var. alkalophilus, was overproduced in the extracellular compartment. A pH-stat fed-batch cultivation of the recombinant E. coli was also performed to achieve the secretive overproduction of $\beta$-CGTase at a high cell density, resulting in production of up to 21.6 U/ml of $\beta$-CGTase.

• Proceedings of the KIEE Conference
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• 2000.07d
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• pp.2992-2994
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• 2000

In cope with insufficient agricultural labor and requirement of high quality product Hydroponics is a really good method. It makes the high density agriculture possible and all the growing environments controllable. So its research is so much progressing to maximize the quantity and quality of farm products. Furthermore, the big progress, in the research of a future agriculture. is systematically conducted for the automatic controlled system. In this paper, a practical automatic control cultivation system is implemented. To automatically control and optimize the very nonlinear and time-varying growth of farm products, a hybrid strategy(FECA: Fuzzy Expertized Control Algorithm) is proposed which serially combines a fuzzy expert system with the fuzzy logic control. The fuzzy expert system (FMES: Fuzzy Model-based Expert System) is intended to overcome the non-linearity of the growth of farm products. The part of fuzzy controller is incorporated to solve the time-variance of the growth of farm products. Finally. the efficiency and the effectiveness of the implemented agricultural automation system is presented through the cultivation results.

• Journal of Bio-Environment Control
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• v.24 no.3
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• pp.173-177
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• 2015

This experiment was conducted to determined the optimum planting density for the production of high quality bitter gourd (Momordica charantia L.) adapted in spring cultivation with the unheated greenhouse condition. 'Erave' variety was planted at three different planting densities (235, 305, $380plants{\cdot}10a^{-1}$) on March 26. The training method was six lateral vines with pinching the main one. The light intensity was lower in the higher planting density than the lower one. Net photosynthetic rates of the bitter gourd leaves in the higher density were significantly lower (41 to 71%) than the lower one. There was no difference in the fruit characteristics among treatments. But the root weight was heavier in the lower planting density ($235plants{\cdot}10a^{-1}$) as 113.1g than 96.0g of the higher planting density ($380plants{\cdot}10a^{-1}$). The number of the harvested fruit also higher in the lower planting density ($235plants{\cdot}10a^{-1}$) with 60.7 than 39.9 of the higher planting density ($380plants{\cdot}10a^{-1}$). The average fruit weight was the highest in the plot of $305plants{\cdot}10a^{-1}$ as 338.7g and lowest in the lower planting density ($235plants{\cdot}10a^{-1}$) as 285.2g. The total yield of $305plants{\cdot}10a^{-1}$ density was $5,359kg{\cdot}10a^{-1}$, which was higher than $4,068kg{\cdot}10a^{-1}$ of the lower planting density ($235plants{\cdot}10a^{-1}$). Marketable yield was increased by 24% in the planting density of $305plants{\cdot}10a^{-1}$($4,767kg{\cdot}10a^{-1}$) as compared to the lower density in $235plants{\cdot}10a^{-1}$($3,629kg{\cdot}10a^{-1}$) and increased by 13% in the planting density as $380plants{\cdot}10a^{-1}$($4,137kg{\cdot}10a^{-1}$). Therefore, the planting density of bitter gourd was desirable in $305plants{\cdot}10a^{-1}$ density for the higher yield and quality in the protected cultivation.