• 제목/요약/키워드: high content screening

검색결과 125건 처리시간 0.028초

Is It Possible to Predict the Iron Status from an Infant's Diet History?

  • Kim, Hyung Jin;Kim, Dong Hyun;Lee, Ji Eun;Kwon, Young Se;Jun, Yong Hoon;Hong, Young Jin;Kim, Soon Ki
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • 제16권2호
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    • pp.95-103
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    • 2013
  • Purpose: Iron deficiency remains a very common nutritional problem despite the improvement in nutrition and increased understanding of methods for its prevention. Thus, we try to create a new method for screening iron nutrition through infant nutrition history. Methods: Among the children who visited Inha University Hospital from March 2006 to July 2012, 181 children with iron deficiency anemia (IDA) and 52 children without IDA ranging from 6 to 36 months of age were reviewed in this study. We used the age when they began to wean food, the type of sort weaning foods, the time required for successful weaning, iron content in weaning foods, and the duration of breastfeeding for scoring infant nutrition history based on a questionnaire. Results: The mean score of the IDA group was $7.8{\pm}2.6$ points, which was significantly higher than that of the control group ($5.6{\pm}2.1$) (p=0.000). If we set up the cutoff value at 6 points, this screening has 86.8% sensitivity and 36% specificity. In addition, as the IDA score increased, there was a falling trend of hemoglobin. Conclusion: The IDA score does not have high specificity or high sensitivity. However, this study conveys that those patients who record a high score have low hemoglobin. Therefore, we suggest this score system for screening more IDA patients via nonpainful techniques.

초음파를 이용한 이대와 왕대 잎으로부터 항산화물질 Homoorientin의 추출효율 향상 (Enhanced effect extraction of Antioxidant substance Homoorientin from Pseudosasa japonica and Phyllostachys bambusoides leaves using Ultrasonic wave system)

  • 이광진;신용국;김영식
    • KSBB Journal
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    • 제24권2호
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    • pp.189-194
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    • 2009
  • 대나무 (이대와 왕대) 잎으로부터 HPLC on-line ABTS screening기법을 사용하여 flavone C-glycosides의 homoorientin의 항산화활성을 빠르게 분석하였으며, 이대와 왕대 잎으로부터 homoorientin의 추출을 다양한 초음파 에너지와 시간의 추출방법을 적용하였다. 전 처리한 추출액에 포함된 이대와 왕대 잎으로부터 homoorientin을 분석하고 최적의 추출조건을 실험적으로 모색하였다. 실험결과에 의하면 왕대는 이대 잎보다 homoorientin의 함량이 7배 정도 높았으며, 최적 추출은 35 kHz, 60 min에서 (positive) 피크면적 1574.71, 23.67%와 (negativity) 피크면적 6924.34, 1.23%로 왕대 잎에서의 추출 효율이 가장 우수 하였다.

감마-리놀렌산 생산 곰팡이의 탐색 (Screening of ${\gamma}-Linolenic$ Acid-Producing Fungi)

  • 신용철;신현경
    • 한국식품과학회지
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    • 제20권5호
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    • pp.724-731
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    • 1988
  • 감마-리놀렌산 생산을 목적으로 균주 보관소에서 구입한 Mucorales목 15균주와 실험실에서 분리한 사상균 10균주의 균체성장, 지질생성량, 감마-리놀렌산 함량 및 펠렛 크기등을 비교 조사하였다. 조사한 균주중 토양에서 분리한 FB-354균주가 진탕배양시 균체성장이 양호하고, 지질함량이 건조균체중 29.9%, 감마-리놀렌산의 함량이 총 지방산중 16.8%로 높고 직경 1mm미만의 적은 펠렛을 형성하는 등 감마-리놀렌산의 생산균주로서의 양호한 특성을 나타내었다. 이 균주는 형태학적 특성을 조사한 결과 Mucor sp.로 동정되었다. 이 균주가 생산하는 지질은 81.1%가 중성지질, 7.2%가 당지질 그리고 11.8%가 인지질로 밝혀졌으며, 감마-리놀렌산은 당지질에는 거의 없고 중성지질에 전체량의 대부분이 존재하였으며 인지질에는 인지질 획분 지방산의 21.4%를 차지하여 상대적으로 다량 존재한 것으로 나타났다.

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의학적 충고에 반한 퇴원의 특성과 퇴원결정 요인에 관한 연구 - 사회사업가의 개입사례와 역할을 중심으로 - (A Study on the Characteristics of DAMA(Discharge Against Medical Advice) Case and Causal Factors of DAMA - Perspective of Medical Social Worker's Role and Intervention -)

  • 강흥구;이상진;조경기
    • Journal of Korean Neurosurgical Society
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    • 제29권12호
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    • pp.1620-1627
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    • 2000
  • Objectives : DAMA cases were analyzed to examine what the main casual factors of DAMA were and how to deal with these cases effectively in hospital with the DAMA interdisciplinary team including medical social worker whose role is to perform psycho-social assessment, family counsel, to evaluate family's DAMA need. Patients and Methods : The content analysis of medical record and social work record were reviewed in 37 cases referred by medical doctor to DAMA team. These cases were reported by patients' self discharge request or family's request for discharge from September 1998 to February 2000. The DAMA team consists of Assistant Director of Hospital as team leader, medical staff in-charge, social worker, QI nurse, other staff members who are not involved in direct treatment for patient, and administrative clerk. Results : The results of content analysis are as follows : 1) The most causal factors of DAMA consist of combination of more than 2 factors. 2) The major decision-maker is revealed to be son and daughter of patient. 3) In 59.4% of cases, family was not informed of patients' prognosis, alternatives, the consequence of DAMA at all. 4) In cases of DAMA report, the rapid intervention of social worker is carried out. Conclusion : In this study, we propose the interdisciplinary team approach to make decision legitimately and ethically for DAMA. The suggestions from this study are as follows : 1) To deal with DAMA case properly, the interdisciplinary team approach should be considered. 2) The criteria for DAMA case should be formed carefully. For the explicit selection of DAMA case, preliminary system for high-risk patient screening is recommended. 3) The medical social worker is available for the psycho-social problems of the patient once family members. For the effective family counselling, discharge planning and nursing home placement, the participation of medical social worker should be mandatory.

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Evaluation of Provider Skills in Performing Visual Inspection with Acetic Acid in the Cervical Cancer Screening Program in the Meknes-Tafilalet Region of Morocco

  • Selmouni, Farida;Sauvaget, Catherine;Zidouh, Ahmed;Plaza, Consuelo Alvarez;Muwonge, Richard;Rhazi, Karima El;Basu, Partha;Sankaranarayanan, Rengaswamy
    • Asian Pacific Journal of Cancer Prevention
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    • 제17권9호
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    • pp.4313-4318
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    • 2016
  • Background: This study documented the performance of providers of visual inspection with acetic acid (VIA) at primary health centers, assessing their compliance with the VIA skills checklist and determinants of non-compliance, and exploring their perceptions of VIA training sessions. Materials and Methods: A cross-sectional study was conducted among VIA providers in the $Mekn\grave{e}s$-Tafilalet region of Morocco. Structured observation of their performance was conducted through supervisory visits and multiple focus group discussions (FGDs). Results: Performance of all the recommended steps for effective communication was observed in a low proportion of procedures (36.4%). Midwives/nurses had higher compliance than general practitioners (GPs) (p<0.001). All recommended steps for VIA examination were performed for a high proportion of procedures (82.5%). Compliance was higher among midwives/nurses than among GPs (p<0.001) and among providers in rural areas than those in urban areas (p<0.001). For pre-VIA counselling, all recommended steps were performed for only 36.8% of procedures. For post-VIA counseling, all recommended steps were performed in a high proportion (85.5% for VIA-negative and 85.1% for VIA-positive women). Midwives/nurses had higher compliance than GPs when advising VIA-positive women (p=0.009). All infection prevention practices were followed for only 14.2% of procedures, and compliance was higher among providers in rural areas than those in urban areas (p<0.001). Most FGD participants were satisfied with the content of VIA training sessions. However, they suggested periodic refresher training and supportive supervision. Conclusions: Quality assurance of a cervical cancer screening program is a key element to ensure that the providers perform VIA correctly and confidently.

Screening of the total phenol content and analysis of phenolic compound in rice (Oryza saiva L.) genetic resources

  • Lee, Ji-Hee;An, Min-Jeong;Kim, Seung-Hyun;Chung, Ill-Min
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.290-290
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    • 2017
  • Rice (Oryza sativa L.) is one of the most consumed staple food crop which is energy source as carbohydrate and also is considered as the important antioxidant sources including various phenolic compounds. According to the increasing demand of healthy life, the concern to antioxidant also is increasing because of its health-promoting effect. Phenolic compounds are one of the plant secondary metabolites class, which shows various benefits to preventing or treating chronic diseases. In this study, we have measured the total phenol content from total 647 rice samples using the Floin-Ciocalteau method, and then were selected 30 rice genetic resources classified with high, middle, and low group on the basis of total phenol content. The average of the total phenol content of each group was high-group ($6892.9{\pm}488.5{\mu}g\;GAE/g$) > middle-group ($1428.1{\pm}76.0{\mu}g\;GAE/g$) > low-group ($97.6{\pm}11.4{\mu}g\;GAE/g$). The selected rice samples were analyzed with LC-MS/MS to find the composition and concentration of individual phenolic in rice grain. High-group and middle-group contained large amounts of protocatechuic acid and (+)-catechin whereas low-group showed limited amount. Among high-group samples, rice samples with black pericarp color (IT 174089, IT 220079, and IT 259958) had high content of peonidin-3-O-glucoside. Further, these black rice samples were special since polydatin, rarely found stilbenoid in rice grain, was detected. Overall, both the sum of phenolic acid and the sum of flavonoid were high-group > middle-group > low-group. Also, each group exhibited different phenolic compositions; high-group consisted of flavonoid more than phenolic acid, middle-group and low-group was comprised of phenolic acid rather than flavonoid, and non-pigmented rice was composed by fully phenolic acid. The total phenol content had positive relationships with the sum of phenolic compound (r = 0.64), the sum of flavonoid (r = 0.74) at the significance level of p < 0.0001. In addition, protocatechuic acid and quercetin showed positive correlation with above phenolic composition parameters; in order, r = 0.98, 0.65 for protocatechuic acid and r = 0.73, 0.78 for quercetin (p < 0.0001). In conclusion, the total phenol content assay showed the possibility of utilization as a phenolic composition indicator in rice grain. Also, this result was suggested study pigment on other material.

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Antioxidant Characteristics in the Leaves of 14 Coniferous Trees under Field Conditions

  • Han, Sim-Hee;Lee, Jae-Cheon;Lee, Wi Young;Park, YoungKi;Oh, Chang-Young
    • 한국산림과학회지
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    • 제95권2호
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    • pp.209-215
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    • 2006
  • We investigated antioxidant capacity in leaves of 14 coniferous trees under field conditions. We focused on understanding the species characteristics on antioxidant systems and screening the coniferous tree species with the best antioxidant systems using their characteristics. The antioxidant capacity of 14 coniferous trees was divided into three groups. First group was Thuja orientalis and Chamaecyparis obtusa and those species had the highest content of ${\beta}$-carotene and xanthophyll. Second group, C. obtusa and Juniperus chinensis, used antioxidant enzymes to mitigate stress. C. obtusa represented high activity at superoxide dismutase (SOD), glutathione reductase (GR), and peroxidase (POD), and J. chinensis exhibited high activity at SOD, POD, catalase (CAT). Third group employed antioxidant such as ascorbic acid and ${\alpha}$-tocopherol. The antioxidant content of T. orientalis was the highest while that of Pinus parviflora and C. obtusa were the lowest. Few species belonged in three groups simultaneously, and most species belonged in at least one or two groups. In summary, we proposed that C. obtusa and T. orientalis had the highest antioxidant capacity while P. parviflora and P. desiflora for. multicalus had the lowest antioxidant capacity.

Rapid Screening of Phospholipid Biomarker Candidates from Prostate Cancer Urine Samples by Multiple Reaction Monitoring of UPLC-ESI-MS/MS and Statistical Approaches

  • Lim, Sangsoo;Bang, Dae Young;Rha, Koon Ho;Moon, Myeong Hee
    • Bulletin of the Korean Chemical Society
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    • 제35권4호
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    • pp.1133-1138
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    • 2014
  • Ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI- MS/MS) provides a high-speed method to screen a large number of samples for small molecules with specific properties. In this study, UPLC-ESI-MS/MS with multiple reaction monitoring (MRM) was employed to screen urinary phospholipid (PL) content for biomarkers of prostate cancer. From lists of urinary PLs structurally identified using nanoflow LC-ESI-MS/MS, 52 PL species were selected for quantitative analysis in urine samples between 22 cancer-free urologic patients as controls and 45 prostate cancer patients. Statistical treatment of data by receiver operating characteristic (ROC) analysis yielded 14 PL species that differed significantly in relative concentrations (area under curve (AUC) > 0.8) between the two groups. Among PLs present at higher levels in prostate cancer urine, phosphatidylcholines (PCs) and phosphatidylinositols (PIs) constituted the major head group PLs (3 PCs and 7 PIs). For technical reasons, PL species of low abundance may be underrepresented in data from UPLC-ESI-MS/MS performed in MRM mode. However, the proposed method enables the rapid screening of large numbers of plasma or urine samples in the search for biomarkers of human disease.

Influences on the Academic Achievement of General Chemistry Based on the Interest for Chemical Subjects of High School

  • Koo, Min Ju;Park, Jong Keun
    • International Journal of Advanced Culture Technology
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    • 제10권1호
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    • pp.170-179
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    • 2022
  • To find out the effect of interest on chemical content, interest in chemistry, choice of science elective subjects, teaching-learning characteristics that make it difficult to understand contents, academic achievement in general chemistry according to university admission screening, etc. were surveyed on 51 students enrolled in the department of chemistry education at P college of education. As a result of the survey, it was found that the interest in Chemistry I, Chemistry II, and general chemistry increased significantly to 94.6%, 73.7%, and 66.0%, respectively. 39.3% of the students answered that they had a lot of difficulty in understanding general chemistry. The characteristics of learners who make it difficult to understand the content were found to be 'lack of willingness to learn' such as 'lack of understanding chemical concepts' and 'lack of problem solving', due to the lack of 'basic knowledge'. As a way to solve these difficulties, 'expert help' appeared the most. The grades of general chemistry of students who entered the regular admission were relatively higher than those of occasional recruitment.

The validity and reliability of the Healthy Lifestyle Screening Tool

  • Kim, Cheong Hoon;Kang, Kyung-Ah
    • Physical Therapy Rehabilitation Science
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    • 제8권2호
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    • pp.99-110
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    • 2019
  • Objective: The aim of the present study was to develop a valid and reliable scale that measures the healthy life styles among young adults. Design: A methodological study design was employed to develop and validate the Healthy Lifestyle Screening Tool (HLST). Methods: The validity and reliability of the HLST were established in accordance with DeVellis' 8 steps guideline for tool development. The question items were generated based on literature reviews and interviews, which were then classified into 12 categories. The HLST was administered to 272 students attending a Korean university. The reliability was tested using Cronbach's alpha. The validity of the scale was examined with the mean inter-item correlations (MIIC) and factor analysis, and was also examined for content validity by experts. Results: The reliability of the HLST was found to be acceptable, as indicated by a Cronbach's alpha of 0.71. In the validity test, items with less than 80% "agreement" ratings on the content validity index by experts were revised. The MIIC values were greater than 0.25. A factor analysis of 36 items extracted 9 factors (i.e., four items per factor), which together explained 50.4% of the variance. The HLST consists of 36 items that measure 9 factors based on a 4-point Likert rating scale, with 4 items per factor, as follows: sunlight, water, air, rest, exercise, nutrition, temperance, trust, and general physical condition. High scores on the HLST are indicative of a healthy lifestyle (HL). Conclusions: The HLST is a valid and reliable scale that can be used to measure HL among young adults. Identification of HL by using the HLST can provide guidance to integrated therapeutic approaches along with conventional physical therapy.