• Title/Summary/Keyword: hibernation

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The Study in the Composition Changes of Muscle Proteins and Amino Acids in the Hibernant Fish-Mudskipper (Boleophthalmus pectinirostris) before and after Hibernation (동면 전ㆍ후 짱뚱어의 육단백질 및 아미노산 조성의 변화에 관한 연구)

  • 박일웅
    • The Korean Journal of Food And Nutrition
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    • v.16 no.3
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    • pp.209-217
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    • 2003
  • The composition of muscle proteins and free amino acids in the mudskipper (hibernant fish) were investigated before and after hibernation (maturity period: August, hibernation period: November thru. April). It was found that crude proteins were 17.6% in August, 17.5% in November and 16.9% in April, while among the muscle proteins, sarcoplasmic proteins were constituted up to 19.2~20.4%, 58.8~61.3% for myofibrilla proteins, 11.2~13.2% for intracellular proteins and 7.5~8.3% for stroma proteins. Composition changes of the muscle proteins were hardly noted until November but during the hibernation(from Nov. to Apr.) the amount of the sarcoplasmic proteins and the myofibrillar proteins decreased pronouncedly. As for the sarcoplasmic proteins, 14 subunits were found and among them, the amount of 30 kDa and 46 kDa subunits were found to increase slightly in April compared with those in November, while the amount of 35 kDa and 65 kDa subunits were decreased slightly. As for the myofibrilla proteins, 13 subunits were found and detectable changes in their composition were not observed until November but in April the amount of myosin heavy chain was increased by 3%, while the amount of actin decreased by 3% when those are compared with the results in November. The composition of amino acids in the muscle proteins was hardly changed during the observation period. But there were considerable changes of composition of free amino acids. Glycine and alanine were found to be the major free amino acids. The most striking feature was the changes in the glycine and arginine content: the former, which is a dominant free amino acid, was increased by two-fold in April compared with that in August and the latter was increased by two-fold in November and by four-fold in April. It was also found that the amount of essential amino acids (i.e., lysine and histidine) and others (alanine, glutamic acid, serine, aspartic acid and valine) increased significantly during the hibernation period.

Seasonal Changes in the Basic Reproductive Physiology of the Endangered Reeves' Turtle (Mauremys reevesii) Based on Hibernation or Nonhibernation (멸종위기 종인 남생이(Mauremys reevesii)의 동면 여부에 따른 기초 번식생리의 계절적 변동)

  • Jung, Yu-Jeong;Kim, Il-Hun;Koo, Kyo-Soung;Song, Jae-Young;Kang, Shin-Geun;Park, Daesik
    • Korean Journal of Environment and Ecology
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    • v.30 no.6
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    • pp.977-985
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    • 2016
  • The captive breeding program of the Reeve's turtle (Mauremys reevesii), which is designated as an endangered category II species by the Korean Ministry of Environment, has developed, but the reproductive physiology of the species is still not fully understood. In this study, we investigated seasonal changes in the sex steroid levels, the number and size of follicles and eggs and male-female interactive behaviors of hibernated and non-hibernated turtles between November 2014 and October 2015. Plasma estradiol level in females increased from July and peaked in the period from August to October. Plasma testosterone level in males peaked between August and September and was the lowest in March, although there were some variations depending on the patterns of hibernation. Hibernated females had fully developed eggs between early May and middle July and laid eggs between early June and late July, but non-hibernated females had eggs between early April and early June and also between middle August and late November, and laid eggs in early June, mid-October, and early February. These results suggest that hibernation could be essential for normal follicular and egg developments. Male-female interactive behaviors including courtship intensified from late August and peaked in October and November. Our findings suggest that mating period of this species is directly related to increased steroid hormone levels, but follicular and egg developments in females are not. Our results could be very useful to the successful operation of captive rearing programs of the endangered Reeve' sturtles.

동면 동물의 혈액상에 관한 연구

  • 노영복
    • The Korean Journal of Zoology
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    • v.31 no.4
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    • pp.251-264
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    • 1988
  • 동면 상태에서 혈액성분의 생화학적 변화치를 조사하고자 전남 각지에 서식 또는 광주직할시 근교에서 양식하고 있는 뱀장어, 개구리, 살모사 그리고 다람쥐를 활동기(7월∼8월), 잠입기(10월∼11월), 동진기(12월∼1월) 및 각려기(3월∼4월)에 채집하였다. 이들 동물들의 혈청에서 Total protein, Albumin, Globulin, A/G ratio, Glucose, Calcium, Urea함유량을 조사하여 얻은 성적을 비교 검토하여 다음과 같은 결론을 얻었다. 1 . Total protein의 변화는 각 동물사이에서 상당한 차이를 보이되 잠입기에 다소 증가하였다. 특히 다람쥐에서 Total protein 함유량이 다른 동물들 보다 많았다. Albumin함유량은 잠입기에 상승하였고 동면기에는 약간의 감소를 보이다가 각성기에는 현저한 감소를 보였다. gloulin함유량은 잠입기에 상당히 상승하고 동면기에 약간의 감소를 나타내었으며 각성기 인 봄철에는 많은 증가 현상을 보였다. 2. 혈청 Urea의 함유량은 동면의 각과정에 따라 상당한 차이가 있었다. 즉 잠입기에 약간 상승하고 동면기에는 저하를 보였으며 각성기에는 전 실험동물에서 검출되지 않았다. 3. 혈당의 변화는 잠입기에 대체로 저하하고 동면기에는 심한 저혈당 수준에 이르렀다. 각성기인 봄철에는 다람쥐를 제외하고는 동면기의 혈희량과 유사하였다. 4. 혈청 Calcium함유량의 변화는 활동기에 비하여 잠입기에는 상당한 증가현상을 보였으나동면기에는 전 실험동물이 거의 같은 수준으로 감소를 보이며 각성기에 까지 감소하였다. In order to the biochemical change of the serological components of hibernating animals, eels(hnguillo joponica Temminck et Schlegel), frogs (Rana nigromacurata Hallowell), snakes (Agkistro-don holds breuicaudus Stejneserl, squirrels (Tamias striatus Linnaeus) which were collected around Gwangiu city and the Chunnam area were observed in accordance with the phases of hibernation. The author described the hibernating cycle as active (June-July), prehibematins(October-Novemberl, hibernating (December-January) and posthibemating phase (March-Aprill. The values of total protein, albumin, globulin, hJG ratlo, calcium, glucose and urea were chiefly investigated in their serum. The results are as following: Total protein was shown to be consider-able in eels, frogs, snakes, squirrels and increased in prehibemation. Especially, the mean value was high in sqirrels. Albumin values was increased during prehibemation. But a little decrease was shown during hibernating and an outstanding decrease during posthibemation. The globulin value increased considerably during prehlbemation but decreased a little during hibernation phase. Changes of urea were shown to be considerable according to the stage of each hibem3-tion. A little increase was shown during prehibemation and decrease during hibernating stage. but urea was not found in posthibemation in any experimental animals. The glucose value was generally decreased during prehibematlon. An extremely little increase in glucose was shown during hibernation (hypoglycemia). All hibernating values except those for squirrels in spring posthibemating phase were at almost the same level. The calcium value was shown to be considerable during prehibemation in comparison with active phase. The same level of decrease was represented in all kinds of animals and continued at the stage of posthibemation.

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Cell Differentiation and Ultrastructure of the Seminiferous Epithelium in Myotis macrodactylus (큰발웃수염박쥐 (Myotis macrodactylus)의 정상피세포의 분화와 미세구조)

  • Lee, Jung-Hun
    • Applied Microscopy
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    • v.33 no.1
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    • pp.25-39
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    • 2003
  • Cell differentiation and ultrastructural characteristics in the seminiferous epithelium of Myotis macrodactylus was investigated with the light and electron microscopes. Spermatogenesis has begun at April and finished at September. The nuclei of A spermatogonia (dark and pale type of spermatogonia) were oval, applied to the basal lamina, and surrounded by Sertoli cells. By comparison with other types of spermatogonia, the cell and nucleus of B type of spermatogonium is globular and larger than A types of spermatogonia. The nucleolus appears as a coarse and touches the nuclear membrane. The cell and nucleus of spermatocytes was globular and larger, but primary spematocyte is larger than secondary spermatocyte. Spermiogenesis was divided according to the level of fine structural difference, into Golgi, cap, acrosomal, maturation and spermiation phases; Golgi, cap, acrosomal and spermiation phases were further subdivided into steps of early and late phase respectively, and maturation phase has only one step. Hence, the spermiogenesis has been divided into a total of nine phases. In the change of karyoplasm, the chromatin granules are condensed at late Golgi phase and completed at spermiation phase. The sperm tail began to develop in early Golgi phase and completed in spermiation phase. The process of degeneration of spermatogenic cells in the seminiferous tubules was continually observed from October, before the beginning of hibernation, to hibernation phase (November, December, January, February, March). Immatured spermatogenic cells in the seminiferous tubules have been engulfed by phagocytosis of Sertoli cells during period of degeneration. It is deduced that the adaptative strategy serves as the mechanism to regulate the effective use of energy to prepare for long hibernation and regulation of breeding cycle.

Hibernation and Seasonal Occurrence of the Cotton caterpillar, Palpita indica(Lepidoptera: Pyralidae), in Watermelon (수박에서 목화바둑명나방(Palpita indica)의 월동과 발생소장)

  • 최동칠;노재종;이기권;김홍선
    • Korean journal of applied entomology
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    • v.42 no.2
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    • pp.111-118
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    • 2003
  • The population density of the Cotton caterpillar, Palpita indica (Saunder) larvae was gradually increased until 10th October and then decreased rapidly after that time at the watermelon culture of rain sheltered house. After 15th October, many larvae went to soil and molted to pupae for the hibernation. Most of pupae were found in pF 2.4 soil moisture condition, but none in pF 1.5 hydro-morphic soil. Most of the pupae were found below 5cm and there was no pupa below 10cm from the soil surface. The sex ratio of the overwintering pupae was 1.09 : 1.00. For the monitoring of the cotton caterpillar adults using sex pheromone compounds, different mixture ratios of each pheromone compound were investigated with (E)-11-hexadecenal and (E, E)-10,12-hexadecadienal. Seven versus three ratio of (I)-11-hexadecenal and (E, E) -10,12-hexadecadienal was more attractive than any other ratio, and then followed by 6 : 4, 8 : 2, 9 : 1, and 5 : 5 mixtures. As a result of monitoring with 7 : 3 mixture of (E)-11-hexadecenal and (E, E)-10,12-hexadecadienal, occurrence peaks of cotton caterpillar adults showed 4 times per year. The first time of adult appearance was late July in 2000 and middle July in 2001 and the highest number was collected on late September.

Temperature, Photoperiod and Illumination for Mating of the European Bumblebee, Bombus terrersis

  • Yoon, Hyung-Joo;Kim, Sam-Eun;Lee, Kyeong-Yong;Leex, Samg-Beom;Park, In-Gyun;Choi, Kyung
    • International Journal of Industrial Entomology and Biomaterials
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    • v.16 no.2
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    • pp.101-107
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    • 2008
  • To improve mating rate of the bumblebee, Bombus terrestris, temperature, photoperiod and illumination during mating periods favorable for B. terrestris were investigated. The mating rate of queen mated at $19^{\circ}C$ was 92.1%, which was 2.1-5.9% higher than that of $22^{\circ}C$ and $25^{\circ}C$. $19^{\circ}C$ was more effective than at $22^{\circ}C$ and $25^{\circ}C$ in death rate during mating periods. The survival rate after hibernation of queen mated at $19^{\circ}C$ was 3.0-17.7% higher than that of $22^{\circ}C$ and $25^{\circ}C$. At the photoperiod regimes during mating periods, queen mated at 14 L was more effective than 12 L and 16 L in death rate during mating, survival rate after hibernation, and egg-characteristics. In case of illumination during mating periods, intensity of over 1000 lux was suitable for mating B. terrestris queen in colony development. Therefore, we supposed that mating temperature favorable for B. terrestris was $19^{\circ}C$ and photoperiod for mating was 14 L, and illumination was over 1000 lux.

A Method of Embedded Linux Light-Weight for Efficient Application Execution (어플리케이션 처리속도 개선을 위한 임베디드 리눅스 경량화 기법)

  • Lee, Tae-Woo;Cho, Ji-Yong;Cho, Yong-Hwan
    • Journal of the Korea Society of Computer and Information
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    • v.18 no.3
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    • pp.1-10
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    • 2013
  • In this paper, we propose a method of embedded linux light-weight to improve efficiency of application running on embedded systems. Three methods including fast booting scheme applying the Hibernation technique, JFFS2 file system optimization applying the Symbolic Link and virtual address mapping, kernel light-weight that guarantees the general purpose was applied. Since then check the system dependency and generate kernel image according to the target embedded kit. And embedded system performance of existing linux and linux which the method proposed in this paper was compared. In experimental result, the kernel size was 9.6% improved and the system booting time was 18% improved. And application processing speed on target embedded kit was improved 11% in the best case, 66% in the worst case. This result show that the light-weight method proposed in this paper is guarantee fast booting time and securing resources and it is good for the application processing speed improvement.