• Parasites, Hosts and Diseases
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• 제26권2호
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• pp.127-132
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• 1988

이형흡충류의 하나인 Stictodora속 흡충 2마리가 구충제 및 하제를 투여한 24세 남자환자의 설 사변에서 검출되었다. 총체는 길이 0.90∼0.98 mm, 폭 0.38 mm로 소형이며 특이한 복흡반-생식만(ventrogenital) 장치가 있고 생식공과 별개인 gonotyl(12∼15개의 미소한 갈고리 보유)을 가지고 있었다. 충체는 형태학적으로 Stictodora fuscatum(Onji and Nishio, 1916)과 매우 비슷하였으나 다수의 충체를 획득할 때까지 확정적인 종 동정은 보류하였다. 환자는 숭어, 농어 등 반염수산 어류를 즐겨 생식한다고 하였다. 이들이 Stirtodora sp.는 물론 따로 보고한 3종류 이형홉충의 감염원이었을 것으로 추측된다. 이 중례는 문헌상 Stictodora속 홉충의 최초 인체기생례에 해당된다.

• Applied Microscopy
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• 제30권2호
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• pp.205-212
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• 2000

Present study was performed to observe the surface ultrastructures of Stictadora furcatum (Trematoda: Heterophyidae). Adult worms were recovered from the cat experimentally infected with metacercariae, and were prepared for scanning electron microscopy. The body was leaf-like and ventrally concave. The oral sucker armed with prominent tegumental spines on the inner surface, and it's lips were obscure. Sensory papillae (type I) in single or grouped forms symmetrically arranged around the oral sucker. The ventrogenital opening retained protruding gonotyl spines and sperms discharged from the genital pore . The body surface was covered with scale-like multipointed tegumental spines. The density and digitated point of spines were gradually decreased toward the posterior end of the body. The digitated points of spines on the anterior portion were 9-12, on the middle were 7-8 and 5-6, and on the posterior were 2-3 and peg-like. Although the tegumental ultrastructure of S. fuscatum was generally similar to those of other heterophyid flukes , the oral sucker with tegumental spines on the inner surface and without obvious lips, and the ventrogenital opening with protruding gonotyl spines were suggested to be the characteristic features.

• Parasites, Hosts and Diseases
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• 제26권1호
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• pp.55-60
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• 1988

이형흡층류의 하나인 Centrocestus armatus의 인체감염 1례가 praziquantel 투여후 성충을 수집, 동정함으로써 확인되었다. 환자는 42세 남자로 경남 산청군에 거주하였으며 상복부 통증, 설사, 소화불량 등을 호소하였다. 환자는 간흡충, 이형흡충류 및 극구흡충 충란 양성이었다. 장흡충류 치료를 위하여 praziquantel 10mg/kg를 투여하였고 MgSO4으로 설사를 유도하고 설사변을 입체해부현미경으로 검사한 바 C. armatus 성충 1마리와 극구흡충 유충 2마리가 수집되었다. 환자는 피라미 등 민물고기를 자주 생식하였다 하며 치료 2∼3주 전에도 피라미를 회로 먹었다고 한다. 따라서 피라미를 비롯한 민물고기가 C. armatus의 감염원이 되었을 것으로 추측되었다.

• Parasites, Hosts and Diseases
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• 제41권2호
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• pp.101-105
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• 2003

The metacercariae of Acanthotrema felis Sohn et at., 2003 (Digenea: Heterophyidae) were discovered in a species of the brackish water fish, Acanthogobius flavimanus, in the Republic of Korea. They were experimentally fed to kittens, and adult flukes were harvested 7 days later. The adults were morphologically characterized by the presence of a bipartite seminal vesicle, the ventral sucker associated with a ventrogenital sac enclosing 3 sclerites (2 long and pointed, and 1 short and thumb-like), and an unarmed gonotyl. The adult flukes were identified as A. felis Sohn et al., 2003, and the brackish water fish A. flavimanus has been verified as one of its second intermediate hosts.

• 대한수의사회지
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• 제48권1호
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• pp.53-63
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• 2012

• Parasites, Hosts and Diseases
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• 제40권3호
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• pp.107-112
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• 2002

A scanning electron microscopic study was performed on the surface ultrastructure of Pygidiopsis summa (Digenea: Heterophyidae) adults. Metacercariae were collected from gills and muscles of mullets (Mugil cephalus) caught in a known endemic area, and adult flukes were harvested from dogs after 8 weeks of experimental infection. The worm was calabash form with its posterior part broader than the anterior part. Tegumental spines were densely distributed over the body surface, except on the suckers and genital apparatus, and around the excretory pore. Well differentiated spines were observed on the anterior half of the body, with 14-16 tips ventrally, and 19-20 tips dorsally. On the oral sucker. three pairs of type I sensory papillae (uni-ciliated knob-like swellings) and one pair of type II sensory papillae (aciliated round-swellings) were observed on the anterior and posterior parts of the lip, respectively. On the lip of the ventral sucker, one pair of type II sensory papillae was distributed only on its posterior part. Sperms were seen emerging from or entering into the genital apparatus. The results showed that the surface ultrastructure of P. summa was unique among the heterophyid trematodes, especially in digitation of tegumental spines and in distribution of sensory papillae on oral and ventral suckers.

• Parasites, Hosts and Diseases
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• 제29권1호
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• pp.55-66
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• 1991

Heterophyidae과에 속하는 Matagenimus yokegawai, Metagonimus Miyata Type, M. takahashii, Heterophyes disbar, H. heterophyes, H. nocens, Heterophyepsis continua, Pygidiopsis summa, Stellanpchasmusfaltatus, Stittodora lari 등 총 21 Operational Taxonomic Unit(OTU)를 사용하여 수리분류학적 연구를 시도하였다. 형질은 절대 측정치가 아닌 상대적 비율을 주로 사용하였으며, 형질수는 26개를 사용하였다. 각 형질간의 유사도를 측정하여 상관계수를 계산한 후 Ward의 방법에 의한 집괴 분석 및 인자 분석을 수행하였다. 분석 결과 Metagonimus 속(genus) 그룹은 Metagonimus yokogawai, Metagonimus Miyata Type, M. takahashii 등 3개의 표현군으로 구분이 되었으며, Metagenimus Miyata Type은 M. takahashii 의 아종 수준으로 분류되었다. Heterophyes 속 그룹은 Heterophyes dispar, H. heterophyes, Jf. nocens 등 명확한 3개의 표현 군으로 구분되며, H. nocens는 H. heterophyes의 아종이 아닌 뚜렷한 종의 수준에서 분류됨을 나타내었다. 그 외의 종들도 역시 뚜렷한 표현 군으로 분류되었으며, 이로부터 흡충류에 있어서도 수리분류학적 방법에 의해 분류가 명화한 분류군의 한계를 규정하는데 많은 도움을 줄 수 있을 것으로 사료된다.

• Parasites, Hosts and Diseases
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• 제57권3호
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• pp.257-264
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• 2019

We tried a series of morphological and molecular approaches to identify a new species of Stellantchasmus (Digenea: Heterophyidae) originating from the wrestling half-beaked fish, Dermogenys pusillus of Thailand. Adult worm samples of the new species were recovered from hamsters experimentally infected with the metacercariae from D. pusillus in Thailand. Two isolates (Thai and Korean) of Stellantchasmus falcatus were used as comparative control groups. Worm samples of 3 Stellantchasmus groups were morphologically observed and molecularly analyzed with the mitochondrial cytochrome c oxidase 1 gene. The morphological characteristics of S. dermogenysi n. sp. are similar to S. falcatus originating from brackish water fish, but minor difference was noted including the absence of the prepharynx, position of the ovary near the ceca end, smaller body size, and shorter esophageal length. A phylogenetic tree derived from neighbor-joining and maximum-likelihood methods suggests that S. dermogenysi n. sp. is separated from S. falcatus supported by high bootstrap values. The relative divergences persist between these host-specific trematodes, which we suggest should be recognized as 2 distinct species. Comparisons of S. dermogenysi n. sp. with S. falcatus isolated from mullets in Thailand and Korea indicate a genetic divergence of mitochondrial DNA of 19.4% and 21.7%, respectively. By the present study, a new species, Stellantchasmus dermogenysi n. sp. (Digenea: Heterophyidae), is proposed in Thailand based on molecular evidences, in addition to minor morphological differences between S. falcatus and the new species.

• Parasites, Hosts and Diseases
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• 제23권2호
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• pp.197-202
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• 1985

Family Heterophyidae에 속하는 흡충류 3종의 성충을 고양이에서 검출함으로써 자연감염을 확인하였다. 1983년 4월 8일부터 1985년 4월 9일 사이에 서을 중앙시장에서 구입한 고양이 181마리의 소장 및 내부장기를 부검한 결과 3마리의 고양이에서 Heterophyopsis continua Onji et Nishio, 1916 성충 5마리 (1∼3마리), 7마리의 고양이에서 Pygidiopsis summa Onji et Nishio, 1916 성충 223마리 (1∼200마리), 3마리의 고양이에서 Heterophyes heterophyes nocens Onji et Nishio, 1916 성충 26마리 (7∼11마리)를 각각 얻을 수 있었다. 이들 중 H. heterophyes nocens를 제외한 2종의 이형흡충류 H. continua 및 P. summa는 국내에서 고양이의 자연감염으로 처음 보고되는 것이다.

• Parasites, Hosts and Diseases
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• 제47권3호
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• pp.315-318
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• 2009

Quantitative fecal egg counts represented as the number of eggs per gram of feces (EPG) are generally a reliable parameter to estimate the worm burden of intestinal and hepatic parasitoses. Although Haplorchis taichui (Digenea: Heterophyidae) is one of the most common minute human intestinal flukes, little is known about the relationship between EPG and the actual worm burden in patients or the severity of the disease. In the present study, fecal samples were collected from 25 villagers in northern Thailand before and after praziquantel treatment. The EPG values of each participant were determined by the modified cellophane thick smear method, and adult worms were collected from the whole stool after the treatment. Eggs per day per worm (EPDPW) of H. taichui were estimated 82 from egg counts and expelled worms. The EPG was not well correlated with the worm burden, and a reverse correlation was observed between the EPDPW and the worm burden.