• Journal of Applied Biological Chemistry
• /
• v.42 no.3
• /
• pp.113-118
• /
• 1999

The transgenic plant of Citrus species (Citrus aurantium L.) was constructed with a fatty acid desaturase gene using microprojectile bombardment transformation system. The DNA of a fatty acid desaturase gene, fad7, constructed in pBI121 was coated onto tungsten particles ($1.1{\mu}m$) and introduced into callus cells by bombarding with 1100 psi of helium pressure, 1/4 in of gap distance, 7.0 cm of target distance and 27 in Hg of chamber vacuum. The bombarded cells were selected on the medium containing kanamycin. The selected cells were successfully regenerated into plantlets via somatic embryogenesis on the media containing plant growth regulators. The results of polymerase chain reaction analysis of genomic DNAs from the putative transformants showed that the introduced DNAs of fad7 were present in both the selected callus cells and the regenerated plantlets.

• Nuclear Engineering and Technology
• /
• v.37 no.6
• /
• pp.587-594
• /
• 2005

In an effort to develop a thermal-hydraulic (TH) safety analysis code for Gas-cooled Reactors (GCRs), the MARS code, which was primarily developed for TH analysis of water reactor systems, has been extended here for application to GCRs. The modeling requirements of the system code were derived from a review of major processes and phenomena that are expected to occur during normal and accident conditions of GCRs. Models fur code improvement were then identified through a review of existing MARS code capability. Among these, the following priority models necessary fur the analysis of limiting high and low pressure conduction cooling events were evaluated and incorporated in MARS-GCR/V1 : 1) Helium (He) and Carbon Dioxide ($CO_2$) as main system fluids, 2) gas convection heat transfer, 3) radiation heat transfer, and 4) contact heat transfer models. Each model has been assessed using various conceptual problems for code-to-code benchmarks and it was demonstrated that MARS-GCR/V1 is capable of capturing the relevant phenomena. This paper describes the models implemented in MARS-GCR/V1 and their verification and validation results.

• Progress in Superconductivity and Cryogenics
• /
• v.11 no.3
• /
• pp.65-68
• /
• 2009

The Stirling cryocoolers have been widely used for the cooling of the infrared detector(InSb, HgCdTe, and etc,) and HTS(High Temperature Superconductor) to the cryogenic temperature. The monobloc Stirling cryocoolers with the rotary compressor are applicable to the cooling device for the compact mobile thermal imaging system, because the cryocoolers have the compact structure and light weight. The typical performance factors of the Stirling cryocooler are the cool-down time, cooling capacity at the desired temperature (80 K), the electric input power and COP. The above performance factors depend on the operating conditions such as the charging pressure of the helium gas, the thermal environment and etc.. In this study, the effects of the thermal environment (temperature of 241, 293, and 333 K) on the performance of the cryocooler were investigated by experiments. The results show the effects of the temperature of the thermal environment on the cooling capacity and input power.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2012.08a
• /
• pp.270-270
• /
• 2012

Low temperature atmospheric pressure plasmas (APPs) have been known to be effective for living cell inactivation in the water [1]. Many earlier research found that pH level of the solution was changed from neutral to acidic after plasma treatment. The importance of the effect of acidity of the solution for cell treatments has already been reported by many experiments. In addition, several studies have demonstrated that the addition of a small amount of oxygen to pure helium results in higher sterilization efficiency of APPs [2]. However, it is not clear yet which species are key factors for the cell treatment. To find key factors, we used GMoo simulation. We elucidate the processes through which pH level in the solution is changed from neutral to acidic after plasma exposure and key components with pH and air variation with using GMoo simulation. First, pH level in a liquid solution is changed by He+ and He(21S) radicals. Second, O3 density decreases as pH level in the solution decreases and air concentration decreases. It can be a method of removing O3 that cause chest pain and damage lung tissue when the density is very high. H2O2, HO2 and NO radicals are found to be key factors for cell inactivation in the solution with pH and air variation.

• Fire Science and Engineering
• /
• v.29 no.5
• /
• pp.29-33
• /
• 2015

In this study, the relations of flow rate, discharging distance and droplet size are measured in accordance with the nozzle shape. The dual type nozzles of LPN142 and LPN148, which have identical core diameter (6.0 mm) and the different radial injection angles (${\theta}_2=142^{\circ}$ and $148^{\circ}$), are manufactured. The distribution diameters with discharging distance are quantified by UL2167 test standard. The relations between discharging angle and droplet sizes, which are measured by the method of Helium-Neon laser equipment, are obtained by the empirical correlation as working pressure increase. Moreover, the extinction coefficient, which is major parameter of the radiative transport equation (RTE) is analyzed with variable droplet sizes. Thus, it is possible to opt the nozzle's shape by analyzing the relations of working pressure, spray distance, droplet size and fire characteristics at minimum allowable flow rate.

• Analytical Science and Technology
• /
• v.15 no.1
• /
• pp.7-14
• /
• 2002

Laser ablation ion trap mass spectrometry (LAITMS) was developed for the analysis of metal and ceramic samples. For this study, XeCl excimer laser (308 nm) was used for ablating the samples and ITMS was used as a detector. Samples were introduced from outside of a ring electrode and this way of sample introduction was very effective for solid samples when laser ablation was employed. Helium gas was used as a buffer gas, and its effect on sensitivity and some parameters (buffer gas pressure, ion storage time, and cut-off RF voltage) were studied. The optimized conditions were $1{\times}10^{-4}$ Torr of buffer gas pressure, 100 ms of ion storage time and $1150V_{p-p}$ of cut-off RF voltage. From that results, copper (Cu) and molybdenum (Mo) metals were tested with LAITMS and the mass spectra of these pure metals were compared with the natural abundance of isotope ratio. We also examined ceramic samples ($Al_2O_3$, $ZrO_2$) and represented the result of elemental analysis.

• Journal of Animal Science and Technology
• /
• v.54 no.3
• /
• pp.191-198
• /
• 2012

The objective of this study was to evaluate the potential use of a dielectric barrier discharge (DBD) plasma system to improve microbial safety of sliced cheese. The atmospheric pressure plasma (APP) effect on visual appearance and a sensory evaluation were also carried out. The number of Escherichia coli inoculated on cheese slices decreased by 0.09, 0.47, 1.16 and 1.47 log cycles with helium (4 liters/min [lpm]) and 0.05, 0.87, 1.89 and 1.98 log cycles with He/$O_2$ mixture (4 lpm/15 standard cubic centimeters per minute), after being treated with plasma for 1, 5, 10, and 15 min, respectively. Significant reductions were also observed in Staphylococcus aureus inoculated onto cheese slices ranging from 0.05 to 0.45 log cycles with He and from 0.08 to 0.91 log cycles with He/$O_2$-treated samples, respectively. Adding oxygen resulted in a significant increase in inactivation of both pathogens. No visible change in the plasma-treated cheese slices was observed even though the instrumental analysis showed a significant decrease in the $L^*$-value and an increase in the $b^*$-value. The cheese slices were damaged after 10 and 15 min of plasma treatment. In addition, significant reductions in sensory quality including flavor, odor, and acceptability of plasma-treated cheese slices were observed. The results indicate that the DBD plasma system has potential for use in sanitizing food products, although the effect was limited. Further development of the APP system is necessary for industrial use.

• Journal of the Korean Society of Propulsion Engineers
• /
• v.11 no.2
• /
• pp.54-61
• /
• 2007

Propellant pressurization system in liquid rocket propulsion system plays a role supplying pressurant gas at a controlled pressure into the ullage space of propellant tanks. The most important design parameter for such propellant pressurization system is the temperature of pressurant gas fed from pressurant tank. Such pressurant is gaseous state, of which density is very sensitive to the temperature of pressurant. Generally for the propulsion system, which requires high thrust and is consisted of cryogenic propellant the pressurant is stored at high density and high pressure to reduce the weight of pressurant tanks, which are placed inside of cryogenic propellant tank. That is called cryogenic storage pressurization system. This study investigates the temperature variation of pressurant at the time when the pressurant is coming out of pressurant tank experimentally as well as numerically. Fluids used in this study are air and liquid oxygen as outer fluid and gaseous nitrogen and gaseous helium as pressurant respectively.

• Geomechanics and Engineering
• /
• v.24 no.6
• /
• pp.519-529
• /
• 2021

In coal mining activities, the abutment stress of the coal has to undergo cyclic loading and unloading, affecting the strength and seepage characteristics of coal; additionally, it can cause dynamic disasters, posing a major challenge for the safety of coal mine production. To improve the understanding of the dynamic disaster mechanism of gas outburst and rock burst coupling, triaxial devices are applied to axial pressure cyclic loading-unloading tests under different axial stress peaks and different pore pressures. The existing empirical formula is use to perform a non-linear regression fitting on the relationship between stress and permeability, and the damage rate of permeability is introduced to analyze the change in permeability. The results show that the permeability curve obtained had "memory", and the peak stress was lower than the conventional loading path. The permeability curve and the volume strain curve show a clear symmetrical relationship, being the former in the form of a negative power function. Owing to the influence of irreversible deformation, the permeability difference and the damage of permeability mainly occur in the initial stage of loading-unloading, and both decrease as the number of cycles of loading-unloading increase. At the end of the first cycle and the second cycle, the permeability decreased in the range of 5.777 - 8.421 % and 4.311-8.713 %, respectively. The permeability decreases with an increase in the axial stress peak, and the damage rate shows the opposite trend. Under the same conditions, the permeability of methane is always lower than that of helium, and it shows a V-shape change trend with increasing methane pressures, and the permeability of the specimen was 3 MPa > 1 MPa > 2 MPa.

• Transactions of the Korean hydrogen and new energy society
• /
• v.33 no.5
• /
• pp.534-540
• /
• 2022

Liquid hydrogen has the best storage capacity per unit mass and is economical among storage methods for using hydrogen as fuel. As the demand for hydrogen increases, the need to develop a storage and supply system of liquid hydrogen is emphasizing. In order to liquefy hydrogen, it is necessary to pre-cool it to a maximum inversion temperature of -253℃. The Gifford-McMahon (GM) refrigerator is the most reliable and commercialized refrigerator among small-capacity cryogenic refrigerators, which can extract high-efficiency hydrogen through liquefied hydrogen production and boil of gas re-liquefaction. Therefore, in this study, the optimal conditions for liquefying gas hydrogen were sought using the GM cryocooler. The process was simulated by PRO/II under various cooling capacities of the GM refrigerator. In addition, the flow rate of hydrogen was calculated by comparing with specific refrigerator capacity depending on the pressure and flow rate of a refrigerant medium, helium. Simulations were performed to investigate the optimal values of the liquefaction flow rate and compression pressure, which aim for the peak refrigeration effect. Based on this, a liquefaction system can be selected in consideration of the cycle configuration and the performance of the refrigerator.