• 한국수산과학회지
• /
• 제29권5호
• /
• pp.620-628
• /
• 1996

여러 다른 S. aureus strains및 토끼항체를 사용하여 항체감작을 시킬 때 나타나는 S. aureus의 자체응집을 방지키 위한 분석과 함께 cogglutination의 최적 조건을 확립하였다. 적정화된 coagglutination기법을 실험실과 현장에서의 edwardsiellosis의 진단에 응용하였을 때 약 $10\;{\mu}g/m1$의 E. tarda까지 검출 할 수 있었다. 더구나 이 방법은 E. tarda의 FKC, EDTA또는 열탕추출 항원에 대해서 까지 좋은 진단결과를 보여주었다. 현장에서 edwardsiellosis에 감염된 어류로부터 직접 분리된 E. tarda 균주들은 토끼 항체생성을 위해 사용된 E. tarda 219와 응집항체법및 cogglutination법에서 모두 교차반응을 보여 주었다. 이러한 교차반응의 정도는 현장에서 나타나는 여러다른 E. tarda 균주에 감염 될 수 있는 어류의 질병진단에 사용하기에 충분한 정도로 나타났으며 감염어의 조직마쇄물을 1000배 이상 희석하여도 토끼 항 E. tarde 항체로 감작시킨 S. aureus와 coagglutination 될수 있는 양의 E. tarda를 함유하고 있었다. 자연감염 또는 인위감염된 넙치, 틸라피아의 조직마쇄물, 열탕추출항원에 대한 이 방법의 적용 결과는 본방법이 특별한 장비없이 현장에서 질병진단 기법으로 사용할 수 있다는 것을 보여 준다.

• 대한미생물학회지
• /
• 제6권1호
• /
• pp.29-40
• /
• 1971

Various kinds of antibiotics are generally believed to have inhibitory effects on the antibody response. However, as polymyxin B which belongs to the cyclic polypeptide group of antibiotic was found to have some enhancing effects on the antibody response of rabbits to enterobacterial common antigen(CA) under specified conditions, experiments were carried out on this problem with the following results. 1. When mixture of polymyxin B and CA derived from Salmonella typhimurium(STM) was treated 30 minutes at $37^{\circ}C$ and injected three times into rabbits by intravenous route, the antibody response to CA was weaker than rabbits injected CA only. 2. Mixture of polymyxin B and CA showed a marked antibody production when injected into rabbits primed with small amounts of heat-extracted antigen of STM, while the injection of CA alone showed low titers of response. 3. Mixture of polymyxin B and heat-extracted CA-containing antigen of Escherichia coli 014 also showed a increased antibody production than CA alone in rabbits primed with antigen of STM. 4. The effect of polymyxin B appeared in different ways. This antibiotic did not enhance the CA antibody response in rabbits primed with small amounts of E. coli 0111 and 055, but enhance in rabbits primed with Shigella flexneri. 5. No enhancing effect on the antibody response was observed by polymyxin B in rabbits primed with CA. 6. No enhancing effect on the antibody response was also noted in rabbits primed with STM antigen in case polymyxin B and CA were administered simultaneously but in veins of different places. 7. Bacitracin did not enhance the CA antibody response in primed rabbits with STM antigen, but neomycin slightly enhance the response. 8. Lipopolysaccharide showed no priming effect on the CA antibody response, and no enhancement of the CA antibody response in rabbits printed with STM. 9. The priming effect of STM antigen against CA antibody response was very weak as compared with the effect of CA derived from STM antigen.