• Title/Summary/Keyword: health science

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Colonization of Pathogens in Earphones and Observation of Effective Sterilization Methods and Cycles

  • Kwon, Hyeokjin;Jeong, Myeongguk;Go, Shinjee;Kim, Yeojin;Kim, Yein;Kim, Yeeun;Roh, Seungjun;Lee, Seonggwang;Choi, Go-Eun
    • Biomedical Science Letters
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    • v.28 no.3
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    • pp.186-191
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    • 2022
  • The use of earphones has recently been widely used around the world. In currently, students wear earphones a lot in a daily life. The types of earphones are open-earphones, Canalphones, and headphones. Many students don't periodically to sterilization their earphones. Therefore, it can be an incubator that can induced ear infections. The objective of this study was to detect the pathogenic bacteria from the earphones used by the students. A total of 3 type earphones swabs were collected by sterile cotton swabs. The swabs were inoculated onto BHI agar and incubated aerobically 48 hour at 37℃. 16s rRNA PCR, electrophoresis and sequencing were performed to confirm the identification of all the bacterial isolates. As a result, 24 pathogens were identified in sequencing. Three types of earphones were sterilized in three ways: ultraviolet (UV), 70% ethyl alcohol, and antibacterial wet tissue. If you use earphones for a long time without disinfecting them for a long time, it causes various diseases such as external ear infections. The findings of this study the users periodically to sterilization their respective earphones.

Evaluating the Efficacy of a Formalin Alternative in Fixing Pathological Tissues for Histological and Molecular Diagnoses

  • Min-A Je;Haneul Lee;Heechul Park;Dong Hyeok Kim;Yeongdon Ju;Jaewon Lim;Sunghyun Kim;Jungho Kim
    • Biomedical Science Letters
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    • v.29 no.1
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    • pp.48-52
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    • 2023
  • Formaldehyde use is associated with serious health risks, which can affect medical personnel and technicians. Therefore, we investigated the efficacy of an alternative fixative, with respect to two types of formalin fixatives, by hematoxylin and eosin (H&E) staining, periodic acid Schiff (PAS) staining, immunohistochemical (IHC) staining, and RNA extraction. For H&E staining, the circular nucleus was stained dark blue by the basic dye hematoxylin and the cytoplasm was stained red by the acid dye eosin in all three fixative samples. No difference was found in the Duksan General Science (DGS), Sigma-Aldrich, and Core-Fix fixative samples (Corebiotech) used to fix kidney tissue, after PAS staining. IHC staining showed that CD4 was significantly increased in the lippolysaccharide (LPS)-treated group compared to the control group (vehicle), confirming the changes in specific molecules. The quantity and quality of RNA from tissues fixed in the three types of fixatives were evaluated. The average concentration of RNA was 106 ng/µL and average purity at A 260/280 ratio was 1.7~2.0, regardless of fixative used. For quality of protein, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was confirmed by Western blotting. In conclusion, Core-Fix can be used as a fixative for pathological tissues, in histological and molecular diagnoses.

Comparison of the Marginal and Internal Gap of Metal Coping according to Processing Method of Dental CAD/CAM System (치과 캐드캠 시스템의 가공 방식에 따른 금속 코핑의 적합도 비교)

  • Kim, Dong-Yeon;Jeon, Jin-Hun;Park, Jin-Young;Kim, Ji-Hwan;Kim, Hae-Young;Kim, Woong-Chul
    • Journal of dental hygiene science
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    • v.15 no.1
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    • pp.12-17
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    • 2015
  • The purpose of this study was to evaluate the marginal and internal gap of metal coping fabricated using additive manufacturing (AM) group and subtractive manufacturing (SM) group by dental computer-aided design (CAD)/computer-aided manufacturing (CAM) systems. Twenty same cases of stone models of abutment teeth 16 by the universal numbering system were manufactured and scanned. Ten metal copings of control group were fabricated using SM and ten metal coping of experimental group were fabricated using AM. Marginal and internal gap of copings were measured using the silicone replica technique and digital microscope (${\times}140$). The data were analyzed using IBM SPSS 21.0 Statistical Software for independent samples t-test (${\alpha}=0.05$). Mean${\pm}$ standard deviation (SD) of marginal and internal gap total size of SM group was $101.00{\pm}40.33{\mu}m$ of AM group was $83.61{\pm}40.37{\mu}m$. Mean${\pm}$SD of marginal and internal gap total size of SM group was significantly greater than that of AM group (p<0.05). This study showed that AM metal copings had a better marginal and internal gap than SM metal copings.

Review of Environmental Health Research through Crowdsourcing (크라우드소싱(crowdsourcing)을 이용한 환경보건 연구 방법의 고찰)

  • Lee, Boram;Lee, Kiyoung
    • Journal of Environmental Health Sciences
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    • v.40 no.3
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    • pp.171-177
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    • 2014
  • Background: The development of technology can be beneficial for the life and health of human society. Crowdsourcing refers to drawing upon a large pool of individuals in order to seek services, ideas, or other contributions. With the development of information communication technology, crowdsourcing is able to provide powerful results in environmental health research. Methods: We searched 'crowdsourcing' and 'citizen science' for keywords related to the environmental health field and only selected journal articles and conference proceedings material, such as research reports and WHO reports. Results: This paper reviewed environmental health research using crowdsourcing. Examples of such research based on crowdsourcing included practices in environmental disasters, noise monitoring, global positioning system (GPS) technology, smart phones, attached portable devices and information delivery by web. Crowdsourcing methods can provide notably distinct approaches for future environmental health research. However, it is also important to protect personal information whenever crowdsourcing is applied to data generation and information dissemination. Conclusion: We expect that this review may provide useful information for the development of new environmental health research methods using crowdsourcing and citizen science.

Bone Mineral Density, Health-promoting Behaviors, and Self-efficacy in Middle-aged Women (일 지역 갱년기 여성의 골 밀도와 건강증진행위, 자기효능감 정도에 관한 연구)

  • Jeong, Geum-Hee;Yang, Soon-Ok;Lee, Kwang-Ok;Pye, Ok-Jung;Lee, Mi-Ra;Baik, Sung-Hee;Kim, Kyung-Won
    • Women's Health Nursing
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    • v.9 no.2
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    • pp.170-178
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    • 2003
  • To identify osteoporosis and examine the relationship between health behavior and the self-efficacy of middle-aged women, a descriptive survey was conducted. The subjects were 465 healthy female residents of Kyunggido, Korea, who underwent ultrasound measurement and a health examination at the Kangmeung-Ci Health Center between July and August 2000. They were 40 to 60 years old, with a mean age of 46.7 years. The subjects completed a self-reported questionnaire that consisted of seven items concerning health behavior and twelve items concerning self-efficacy related to osteoporosis. Bone density was evaluated from ultrasound measurements of the right heel. All the data were analyzed using the program SAS-PC. The study found that the mean T score related to bone density was -1.30, and ranged from -3.52 to 3.06. Based on the T score, 74.8% of the subjects were normal, 12.9% had osteopenia, and 12.3% had osteoporosis. The mean osteoporosis self-efficacy score was 41.17 and ranged from 12 to 60. Osteoporosis self-efficacy differed significantly with health behavior related to health supplementary food (t=5.63, p=.018), exercise (t=6.65, p=.010), alcohol drinking(t=10.80, p=.001), and smoking (t=10.23, p=.001). A community-based health promotion program should be developed to prevent osteoporosis in middle-aged women.

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The Effects of a Health Promotion Program for Elderly (노인을 위한 건강증진프로그램 적용효과)

  • Han, Sang-Sook;Kim, Won-Ock;Hyun, Kyung-Sun;Won, Jeong-Sook;Kim, Kwuy-Bun
    • Journal of Korean Academy of Nursing
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    • v.35 no.6
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    • pp.1054-1062
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    • 2005
  • Purpose: The purpose of this study was to develop and to evaluate a health promotion program for elderly. Method: Subjects were 63 elderly women (experimental group:33, control group:30). The study was a nonequivalant control group pretest-posttest design. The data was analyzed with an SPSS Window program, then the data was computed for the purpose of each study. Result: 1. In designing the program, the experimental group was given health education - 2 times per week, for 8 weeks - and they participated in recreation programs and stretching exercises five times a week. 2. Stretching exercises including health education, significantly affected the total Cholesterol, HDL-cholesterol, triglycerides, health behavior and self-efficacy. 3. The body fat weight was not significantly affected by the stretching exercises. Conclusion: This program was tested to promote the health of elderly and verified as an effective nursing intervention program, because the outcome of this program ascertains that this program enhances self efficacy of exercise, reduces Cholesterol and triglyceride levels' Increases HDL-cholesterol, and helps promote the understanding of heath behavior.