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Development of an Automatic PCR System Combined with Magnetic Bead-based Viral RNA Concentration and Extraction

  • MinJi Choi;Won Chang Cho;Seung Wook Chung;Daehong Kim;Il-Hoon Cho
    • Biomedical Science Letters
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    • v.29 no.4
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    • pp.363-370
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    • 2023
  • Human respiratory viral infections such as COVID-19 are highly contagious, so continuous management of airborne viruses is essential. In particular, indoor air monitoring is necessary because the risk of infection increases in poorly ventilated indoors. However, the current method of detecting airborne viruses requires a lot of time from sample collection to confirmation of results. In this study, we proposed a system that can monitor airborne viruses in real time to solve the deficiency of the present method. Air samples were collected in liquid form through a bio sampler, in which case the virus is present in low concentrations. To detect viruses from low-concentration samples, viral RNA was concentrated and extracted using silica-magnetic beads. RNA binds to silica under certain conditions, and by repeating this binding reaction, bulk samples collected from the air can be concentrated. After concentration and extraction, viral RNA is specifically detected through real-time qPCR (quantitative polymerase chain reaction). In addition, based on liquid handling technology, we have developed an automatic machine that automatically performs the entire testing process and can be easily used even by non-experts. To evaluate the system, we performed air sample collection and automated testing using bacteriophage MS2 as a model virus. As a result, the air-collected samples concentrated by 45 times then initial volume, and the detection sensitivity of PCR also confirmed a corresponding improvement.

A vibration-based approach for detecting arch dam damage using RBF neural networks and Jaya algorithms

  • Ali Zar;Zahoor Hussain;Muhammad Akbar;Bassam A. Tayeh;Zhibin Lin
    • Smart Structures and Systems
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    • v.32 no.5
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    • pp.319-338
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    • 2023
  • The study presents a new hybrid data-driven method by combining radial basis functions neural networks (RBF-NN) with the Jaya algorithm (JA) to provide effective structural health monitoring of arch dams. The novelty of this approach lies in that only one user-defined parameter is required and thus can increase its effectiveness and efficiency, as compared to other machine learning techniques that often require processing a large amount of training and testing model parameters and hyper-parameters, with high time-consuming. This approach seeks rapid damage detection in arch dams under dynamic conditions, to prevent potential disasters, by utilizing the RBF-NNN to seamlessly integrate the dynamic elastic modulus (DEM) and modal parameters (such as natural frequency and mode shape) as damage indicators. To determine the dynamic characteristics of the arch dam, the JA sequentially optimizes an objective function rooted in vibration-based data sets. Two case studies of hyperbolic concrete arch dams were carefully designed using finite element simulation to demonstrate the effectiveness of the RBF-NN model, in conjunction with the Jaya algorithm. The testing results demonstrated that the proposed methods could exhibit significant computational time-savings, while effectively detecting damage in arch dam structures with complex nonlinearities. Furthermore, despite training data contaminated with a high level of noise, the RBF-NN and JA fusion remained the robustness, with high accuracy.

Abnormal State Detection using Memory-augmented Autoencoder technique in Frequency-Time Domain

  • Haoyi Zhong;Yongjiang Zhao;Chang Gyoon Lim
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.18 no.2
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    • pp.348-369
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    • 2024
  • With the advancement of Industry 4.0 and Industrial Internet of Things (IIoT), manufacturing increasingly seeks automation and intelligence. Temperature and vibration monitoring are essential for machinery health. Traditional abnormal state detection methodologies often overlook the intricate frequency characteristics inherent in vibration time series and are susceptible to erroneously reconstructing temperature abnormalities due to the highly similar waveforms. To address these limitations, we introduce synergistic, end-to-end, unsupervised Frequency-Time Domain Memory-Enhanced Autoencoders (FTD-MAE) capable of identifying abnormalities in both temperature and vibration datasets. This model is adept at accommodating time series with variable frequency complexities and mitigates the risk of overgeneralization. Initially, the frequency domain encoder processes the spectrogram generated through Short-Time Fourier Transform (STFT), while the time domain encoder interprets the raw time series. This results in two disparate sets of latent representations. Subsequently, these are subjected to a memory mechanism and a limiting function, which numerically constrain each memory term. These processed terms are then amalgamated to create two unified, novel representations that the decoder leverages to produce reconstructed samples. Furthermore, the model employs Spectral Entropy to dynamically assess the frequency complexity of the time series, which, in turn, calibrates the weightage attributed to the loss functions of the individual branches, thereby generating definitive abnormal scores. Through extensive experiments, FTD-MAE achieved an average ACC and F1 of 0.9826 and 0.9808 on the CMHS and CWRU datasets, respectively. Compared to the best representative model, the ACC increased by 0.2114 and the F1 by 0.1876.

Time-varying characteristics analysis of vehicle-bridge interaction system using an accurate time-frequency method

  • Tian-Li Huang;Lei Tang;Chen-Lu Zhan;Xu-Qiang Shang;Ning-Bo Wang;Wei-Xin Ren
    • Smart Structures and Systems
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    • v.33 no.2
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    • pp.145-163
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    • 2024
  • The evaluation of dynamic characteristics of bridges under operational traffic loads is a crucial aspect of bridge structural health monitoring. In the vehicle-bridge interaction (VBI) system, the vibration responses of bridge exhibit time-varying characteristics. To address this issue, an accurate time-frequency analysis method that combines the autoregressive power spectrum based empirical wavelet transform (AR-EWT) and local maximum synchrosqueezing transform (LMSST) is proposed to identify the time-varying instantaneous frequencies (IFs) of the bridge in the VBI system. The AR-EWT method decomposes the vibration response of the bridge into mono-component signals. Then, LMSST is employed to identify the IFs of each mono-component signal. The AR-EWT combined with the LMSST method (AR-EWT+LMSST) can resolve the problem that LMSST cannot effectively identify the multi-component signals with weak amplitude components. The proposed AR-EWT+LMSST method is compared with some advanced time-frequency analysis techniques such as synchrosqueezing transform (SST), synchroextracting transform (SET), and LMSST. The results demonstrate that the proposed AR-EWT+LMSST method can improve the accuracy of identified IFs. The effectiveness and applicability of the proposed method are validated through a multi-component signal, a VBI numerical model with a four-degree-of-freedom half-car, and a VBI model experiment. The effect of vehicle characteristics, vehicle speed, and road surface roughness on the identified IFs of bridge are investigated.

Generation of wind turbine blade surface defect dataset based on StyleGAN3 and PBGMs

  • W.R. Li;W.H. Zhao;T.T. Wang;Y.F. Du
    • Smart Structures and Systems
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    • v.34 no.2
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    • pp.129-143
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    • 2024
  • In recent years, with the vigorous development of visual algorithms, a large amount of research has been conducted on blade surface defect detection methods represented by deep learning. Detection methods based on deep learning models must rely on a large and rich dataset. However, the geographical location and working environment of wind turbines makes it difficult to effectively capture images of blade surface defects, which inevitably hinders visual detection. In response to the challenge of collecting a dataset for surface defects that are difficult to obtain, a multi-class blade surface defect generation method based on the StyleGAN3 (Style Generative Adversarial Networks 3) deep learning model and PBGMs (Physics-Based Graphics Models) method has been proposed. Firstly, a small number of real blade surface defect datasets are trained using the adversarial neural network of the StyleGAN3 deep learning model to generate a large number of high-resolution blade surface defect images. Secondly, the generated images are processed through Matting and Resize operations to create defect foreground images. The blade background images produced using PBGM technology are randomly fused, resulting in a diverse and high-resolution blade surface defect dataset with multiple types of backgrounds. Finally, experimental validation has proven that the adoption of this method can generate images with defect characteristics and high resolution, achieving a proportion of over 98.5%. Additionally, utilizing the EISeg annotation method significantly reduces the annotation time to just 1/7 of the time required for traditional methods. These generated images and annotated data of blade surface defects provide robust support for the detection of blade surface defects.

Analysis of Recipes for Korean Foods in Web Sites (레시피 관련 웹 사이트 중 한국음식 레시피의 자료 분석 및 검토)

  • Yun, Mi-Ok;Mun, Hyeon-Gyeong
    • Journal of the Korean Dietetic Association
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    • v.10 no.4
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    • pp.390-400
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    • 2004
  • Food and nutrition sites are the major portion of the health information sites. For the point of public health it is very important to secure validity and reliability of information on those web sites. Therefore, in this study we would like to identify problems when acquiring recipes in web sites by analyzing and reviewing recipes in web sites. To investigate Korean food recipes provided in web sites, domestic search engines such as Simmani, Naver, Hanmir, and Empas and foreign search engines such as Yahoo Korea, Lycos and Altabista Korea were used. Searchs were done using 'recipe' and 'Joribeob (cooking method)' from March 20, 2002 to June 20, 2002. Informations in each sites were reviewed and analyzed Results are as follow; When classifying 46sites searched with 'Joribeob' by the information provider, 24sites were individual, 16sites were corporate and 6sites were others. When searching 'recipe', total 12,654recipes were returned. Out of them, individual provided 2,581sites(20.4%), corporate provided 7,249sites(57.3%), and others provided 2,824sites(22.3%). 9,979(78.9%) recipes out of 12,654recipes were proved to be appropriate as Korean food. Classifying recipes by dish group, vegetables 11.7%, soups and hot soups 9.7%, stew and casseroles 8.2%, pan cakes 8.0%, stir fried foods and skewers 7.8%, rice 7.2%, hard boiled food 7.1%, steam 6.4%, noodles and mandu 5.3%, Kimchi 4.5%, fried 4.1%, and porridge 3.7% in order. 21.1% of recipes were not appropriate as Korean food but provided as Korean Food. The proportion of individual as the information provider were higher than that of enterprises. Recipes from enterprises were based on food and nutrient information and more reliable. However, there were some cases that they provided the same amount of ingredients with different calories or provided the same calories with different ingredients. Additionally, depending on sites, they provided different calories even for the same recipe. There were some cases that the calories provided on the site were too high or too low, for the suggested amount of ingredients and serving size. Recipes those provide amount of calories were evaluated using the nutrient analysis program. Calculated calories and provided calories on the Web were compared together. There are difference between two valus. With these results, it may lead misuse of recipe by those who need accuracy in diet such as patients or who are interested in recipe information for academic purposes. These results could be used as basic materials to improve quantity and quality of recipes in the future. Also, to improve the accuracy of recipies for Korean foods in the web sites, there should be some systems to monitor and let internet users know monitoring results.

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Market in Medical Devices of Blockchain-Based IoT and Recent Cyberattacks

  • Shih-Shuan WANG;Hung-Pu (Hong-fu) CHOU;Aleksander IZEMSKI ;Alexandru DINU;Eugen-Silviu VRAJITORU;Zsolt TOTH;Mircea BOSCOIANU
    • Korean Journal of Artificial Intelligence
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    • v.11 no.2
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    • pp.39-44
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    • 2023
  • The creativity of thesis is that the significance of cyber security challenges in blockchain. The variety of enterprises, including those in the medical market, are the targets of cyberattacks. Hospitals and clinics are only two examples of medical facilities that are easy targets for cybercriminals, along with IoT-based medical devices like pacemakers. Cyberattacks in the medical field not only put patients' lives in danger but also have the potential to expose private and sensitive information. Reviewing and looking at the present and historical flaws and vulnerabilities in the blockchain-based IoT and medical institutions' equipment is crucial as they are sensitive, relevant, and of a medical character. This study aims to investigate recent and current weaknesses in medical equipment, of blockchain-based IoT, and institutions. Medical security systems are becoming increasingly crucial in blockchain-based IoT medical devices and digital adoption more broadly. It is gaining importance as a standalone medical device. Currently the use of software in medical market is growing exponentially and many countries have already set guidelines for quality control. The achievements of the thesis are medical equipment of blockchain-based IoT no longer exist in a vacuum, thanks to technical improvements and the emergence of electronic health records (EHRs). Increased EHR use among providers, as well as the demand for integration and connection technologies to improve clinical workflow, patient care solutions, and overall hospital operations, will fuel significant growth in the blockchain-based IoT market for linked medical devices. The need for blockchain technology and IoT-based medical device to enhance their health IT infrastructure and design and development techniques will only get louder in the future. Blockchain technology will be essential in the future of cybersecurity, because blockchain technology can be significantly improved with the cybersecurity adoption of IoT devices, i.e., via remote monitoring, reducing waiting time for emergency rooms, track assets, etc. This paper sheds the light on the benefits of the blockchain-based IoT market.

Study on Combined Use of Inclination and Acceleration for Displacement Estimation of a Wind Turbine Structure (경사 및 가속도 계측자료 융합을 통한 풍력 터빈의 변위 추정)

  • Park, Jong-Woong;Sim, Sung-Han;Jung, Byung-Jin;Yi, Jin-Hak
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.35 no.1
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    • pp.1-8
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    • 2015
  • Wind power systems have gained much attention due to the relatively high reliability, good infrastructures and cost competitiveness to the fossil fuels. Advances have been made to increase the power efficiency of wind turbines while less attention has been focused on structural integrity assessment of structural sub-systems such as towers and foundations. Among many parameters for integrity assessment, the most perceptive parameter may be the induced horizontal displacement at the hub height although it is very difficult to measure particularly in large-scale and high-rise wind turbine structures. This study proposes an indirect displacement estimation scheme based on the combined use of inclinometers and accelerometers for more convenient and cost-effective measurements. To this end, (1) the formulation for data fusion of inclination and acceleration responses was presented and (2) the proposed method was numerically validated on an NREL 5 MW wind turbine model. The numerical analysis was carried out to investigate the performance of the propose method according to the number of sensors, the resolution and the available sampling rate of the inclinometers to be used.

Performance Evaluation of Wireless Sensor Networks in the Subway Station of Workroom (지하철 역사내 무선 센서네트워크 환경구축을 위한 무선 스펙트럼 분석 및 전송시험에 관한 연구)

  • An, Tea-Ki;Kim, Gab-Young;Yang, Se-Hyun;Choi, Gab-Bong;Sim, Bo-Seog
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.7
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    • pp.3220-3226
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    • 2011
  • In order to monitor internal risk factors such as fire, terror, etc. on the subway station, the surveillance systems using CCTV and various kinds of sensors have been implemented and recently, introduction of surveillance systems using an advanced IT technology, sensor network technology is tried on several areas. Since 2007, Korean government has made an effort to develop the intelligent surveillance and monitoring system, which can monitor fire, intrusion, passenger congestion, health-state of structure, etc., by using wireless sensor network technology and intelligent video analytic technique. For that purpose, this study carried out field wireless communication environment test on Chungmuro Station of Seoul Metro on the basis of ZigBee that is considered as a representative wireless sensor network before field application of the intelligent integrated surveillance system being developed, arranged and analyzed and ZigBee based wireless communication environment test results on the platform and waiting room of Chungmuro Station on this paper. Results of wireless spectrum analysis on the platform and waiting room showed that there is no radio frequency overlapped with that of ZigBee based sensor network and no frequency interference with adjacent frequencies separated 10MHz or more. As results of wireless data transmission test using ZigBee showed that data transmission is influenced by multi-path fading effect from the number and flow rate of passengers on the platform or the waiting room rather than effects from entrance and exit of the train to/from the platform, it should be considered when implementing the intelligent integrated surveillance system on the station.

Development of an Intelligent Illegal Gambling Site Detection Model Based on Tag2Vec (Tag2vec 기반의 지능형 불법 도박 사이트 탐지 모형 개발)

  • Song, ChanWoo;Ahn, Hyunchul
    • Journal of Intelligence and Information Systems
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    • v.28 no.4
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    • pp.211-227
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    • 2022
  • Illegal gambling through online gambling sites has become a significant social problem. The development of Internet technology and the spread of smartphones have led to the proliferation of illegal gambling sites, so now illegal online gambling has become accessible to anyone. In order to mitigate its negative effect, the Korean government is trying to detect illegal gambling sites by using self-monitoring agents or reporting systems such as 'Nuricops.' However, it is difficult to detect all illegal sites due to limitations such as a lack of staffing. Accordingly, several scholars have proposed intelligent illegal gambling site detection techniques. Xu et al. (2019) found that fake or illegal websites generally have unique features in the HTML tag structure. It implies that the HTML tag structure can be important for detecting illegal sites. However, prior studies to improve the model's performance by utilizing the HTML tag structure in the illegal site detection model are rare. Against this background, our study aimed to improve the model's performance by utilizing the HTML tag structure and proposes Tag2Vec, a modified version of Doc2Vec, as a methodology to vectorize the HTML tag structure properly. To validate the proposed model, we perform the empirical analysis using a data set consisting of the list of harmful sites from 'The Cheat' and normal sites through Google search. As a result, it was confirmed that the Tag2Vec-based detection model proposed in this study showed better classification accuracy, recall, and F1_Score than the URL-based detection model-a comparative model. The proposed model of this study is expected to be effectively utilized to improve the health of our society through intelligent technology.