• 대한한의학회지
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• 제37권1호
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• pp.125-134
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• 2016

Objectives: The aim of this study is to develop diagnostic questionnaires for the lumbar movement system impairment syndrome. Methods: We had reviewed the previous study and literature and organized various checkable items for differential diagnosis of five different lumbar movement system impairment syndromes. Next, we selected items which can be developed as questionnaire items. Finally, we conducted face validity study with eight Korean medicine doctors and survey research for the importance score of the items with three experts. Results: We developed a diagnostic questionnaire as followings: 6 items for lumbar extension syndrome; 5 items for lumbar flexion syndrome; 6 items for lumbar rotation syndrome. With the 2 rounds of survey research, we could bridge the differences of the importance score of each items. Conclusions: Questionnaire for the diagnosis of movement system impairment syndrome was developed. With this questionnaire, objective diagnosis of lumbar movement system impairment syndrome can be expected. This tool may also be used for detecting sub-health status of musculoskeletal systems.

• 대한예방한의학회지
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• 제20권2호
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• pp.17-26
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• 2016

Objectives : We presented the results of reliability study in advance, and analyzed agreement between Korean medicine doctors(KMDs)' diagnosis and cold-heat pattern identification questionnaire(CHPI)'s diagnosis. Methods : This survey was conducted from May 16 to 17, 2015. The subjects were 93 adults living in rural society. Diagnosis of CHPI was performed by 2 KMDs who have clinical experience more than 5 years. The KMDs' diagnosis was set as a reference index, and then we compare 23 items(cold pattern 11 items and heat pattern 12 items) of CHPI questionnaire and 15 items(cold pattern 8 items and heat pattern 7 items) that were brief form of it. We had cut-off value by standard of KMDs' diagnosis using receiver operating characteristic-curve(ROC-curve), with which we calculated agreement including kappa value. Correlation analysis between CHPI evaluation score by KMDs and by the questionnaire was fulfilled as well. Results : Agreement about 11 and 8 cold pattern items showed 87.1% together, and the value of kappa each recorded 0.742 and 0.741. Agreement about 12 and 7 heat pattern items suggested 81.7% and 78.5%, and the value of kappa showed 0.634 and 0.570. Correlation coefficients were 0.803 of 11 items and 0.761 of 8 items about cold pattern. In addition, correlation coefficients were 0.789 of 12 items and 0.767 of 7 items about heat pattern. The significant probability (p-value) was under 0.001. Conclusions : We have developed CHPI questionnaire involving reliability and agreement based on usual symptoms, and hope additional complements so that Korean medicine diagnostics and Korean preventive medicine would be improved.

• 대한예방한의학회지
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• 제13권3호
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• pp.101-112
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• 2009

Objectives : This comparative study aimed to identify the effect of the Buddeumi(cupping-and-moxibustion device) therapy and moxibustion-only therapy on treating constipation of stroke patients who were admitted in the hospital of oriental medicine. The research design was a nonequivalent control group pretest-posttest design. Methods : The subjects of the study consisted of 23 stroke patients, and they were divided into two groups : one was the experimental group of 11 patients and the other was the control group of 12 patients. The Buddeumi therapy and the moxibustion-only therapy were given respectively. Each Buddeumi and moxibustion-only therapies on the 天樞(ST25, right & left) in abdomen were administered to the each group for 40 minutes a total at 10 times. The posttest included the frequency of defecation with the Constipation Assessment Scale every day for one week after the experiment. The data were analyzed by the $x^2$ test, t-test, Fisher exact test, and repeated measures ANCOVA using SPSS/WIN 12.0 program. Results : The results were as follows : First, Hypothesis 1 : 'The patients who are given the Buddeumi therapy in the experimental group have a lower Constipation Assessment Scale than the control group.' was supported(F=4.367, P=0.049). Second, Hypothesis 2 : 'The patients who are given meridian acupressure in the experimental group have a higher frequency of defecation per week than the control group.' was supported(F=0.947, P=0.034). Accordingly, 'The patients who are given the Buddeumi therapy in experimental group have a higher effect on constipation than the control group.' was supported. Conclusion : The Buddeumi therapy reduced the constipation assessment scale of stroke patients and increased the frequency of their defacation per week. Therefore, the Buddeumi therapy could be an effective treatment for constipation of stroke patients.

• 한국정보전자통신기술학회논문지
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• 제10권5호
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• pp.417-423
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• 2017

인간의 건강에 대한 관심 증가에 맞춘 상시적인 질병 관리를 위해 다수 개의 측정센서를 이용하여 측정된 생체 신호를 융합한 임계값을 분석하여 개개인의 맞춤형 진단을 위한 모니터링 시스템을 구현하고자 한다. 성능평가 결과 생체 신호의 분석을 위한 SVM 알고리즘은 평균 2%의 오차율이 나타났으며, 윈도우의 크기를 5000으로 분할했을 때 저장 공간의 최대 19.2%를 축소함으로써 효과적임을 보였다. 분류의 정확도는 윈도우 크기를 5000으로 분할했을 때 97.2%로 가장 높은 정확도를 보였다. 또한 총 5000개의 생체 신호 집합의 분석 결과 중 84개의 결과가 다르게 나왔으나 시스템으로부터의 결과가 전문가의 진단 결과보다 더 낮은 경우는 발생하지 않았으며, 약 98%의 정확도를 보였다.

• Journal of Microbiology and Biotechnology
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• 제28권5호
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• pp.809-815
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• 2018

Influenza, which is a highly contagious disease caused by the influenza A virus, continues to be a major health concern worldwide. Although the accurate and early diagnosis of influenza virus infection is important for controlling the spread of this disease and rapidly initiating antiviral therapy, the current influenza diagnostic kits are limited by their low sensitivity. In this study, we developed several new influenza nucleoprotein (NP)-specific monoclonal antibodies (mAbs) and compared their sensitivity and specificity of those with commercially available anti-NP mAbs. Three mAbs, designated M24.11, M34.3, and M34.33, exhibited higher reactivities to recombinant NPs and A/Puerto Rico/8/1934 (H1N1) viral lysates compared with the commercial mAbs, as assessed using enzyme-linked immunosorbent assays. M34.3 and M34.33 showed higher reactivities with A/California/04/09 (pandemic H1N1) and A/Philippines/2/82 (H3N2) viral lysates than the commercial mAbs. In contrast, M24.11 had marked reactivity with H3N2 but not with pandemic H1N1. Immunofluorescent confocal microscopy showed that the three mAbs effectively detected the presence of influenza virus in lung tissues of mice infected with A/Puerto Rico/8/1934. These results indicate that the newly developed M34.3 and M34.33 mAbs could be useful for the development of influenza diagnostics.

• 대한환경공학회지
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• 제32권12호
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• pp.1111-1118
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• 2010

비접촉법으로 유동장이나 온도, 화학종 농도의 계측이 가능한 레이저 응용 계측기술은 연소 메카니즘의 해명뿐만 아니라 수치해석 결과의 정당성을 입증하는 수단으로 최근 주목받고 있다. 본 연구에서는 레이저 도플러 유속계와 쉐도우 도플러 입자 분석계를 이용하여 난류 미분탄 화염 내 입자거동에 대하여 관찰하였다. 버너는 여러 광학계측을 용이하게 하기 위하여 대기개방형으로 하였으며, 실험실 규모의 안정된 난류 미분탄화염이 형성 가능한 소형 모델버너를 제작 하였다. 그 결과 미분탄 입자의 평균입경은 연소과정이 진행함에 따라 증가하는 경향을 나타내었다. 이러한 원인은 다수의 소입경의 미분탄 입자가 휘발분을 방출하여 연소반응에 의해 소실되기 때문이다. 또한 화염 중심부에서 미분탄 입자의 속도장은 입경의 크기에 크게 의존하지 않지만, 화염 외주부에서의 미분탄 입자의 속도장은 입경에 크게 의존하고 있음을 알 수 있다.

• 한국추진공학회지
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• 제3권1호
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• pp.24-33
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• 1999

가스터빈 엔진의 성능예측과 진단을 위해 선형 및 비선형 가스경로 해석방법이 적용되었다. 염, 부식, 침식과 같은 물리적 손상을 탐지하기 위한 최적 계측변수를 구하기 위해 비선형 가스경로 해석을 이용하였다. 물리적 손상이 엔진성능에 미치는 영향을 연구하는데 전형적 산업용 가스터빈 엔진인 TB5000에 적용하였다. 선형 가스경로 해석과 비선형 가스경로 해석의 끈 오차 비교를 통해 최적의 계측변수가 정의될 수 있었다. 결과적으로, 선형 가스경로 해석방법은 선형화 모델의 가정에 의해 유도된 오차정도가 손상의 크기와 같은 정도가 되는 반면에 비선형 가스경로 해석방법은 Newton-Raphson 반복기법을 사용하여 독립변수와 종속변수의 비선형 관계를 충분한 정확성과 함께 풀 수 있다는 점을 알 수 있었다.

• 동의생리병리학회지
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• 제23권5호
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• pp.1172-1177
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• 2009

This study is to determine the effects of BUDDEUMI(moxibustion and cupping a boli) therapy on the relief of shoulder pain among middle aged women. methods : A total of 39 middle aged women with shoulder pain are selected : 20 in the experimental group and 19 in the control group. The data was collected by using questionnaires. The BUDDEUMI(moxibustion and cupping a boli) therapy on the should and back was administered to the experimental group, and no treatment was given to the control group. All of the subjects were examined on a subjective symptom scale and on the grade of discomfort and pain in everyday life as developed by Japan's Industrial Fatigue Research Committee of the Industrial Hygienics Society(1988), Sohn, Mong Ho(1999), Visual Analogue Scale as developed by Cline (1992). SPSS/win 12.0 was used for Date analysis, General features demonstrated by frequency number and percentage. The effects of the BUDDEUMI(moxibustion and cupping a boli) therapy was analyzed by a t-test. The homogeneity testing revealed that all study variables are equally distributed between experimental and control groups. The hypothesis, 'the experimental group who received BUDDEUMI(moxibustion and cupping a boli) therapy will decrease in the grade of discomfort of shoulder', is supported. The hypothesis, 'the experimental group who received BUDDEUMI(moxibustion and cupping a boli) therapy will decrease in the Visual Analogue Scale is supported. Conclusion : As a result of this study, BUDDEUMI(moxibustion and cupping a boli) therapy will be able to be used as the self care therapy to improve the symptoms of middle aged women who has the shoulder pain.

• Parasites, Hosts and Diseases
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• 제55권3호
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• pp.233-238
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• 2017

Pathogenic Naegleria fowleri, Acanthamoeba castellanii, and Acanthamoeba polyphaga, are distributed worldwide. They are causative agents of primary amoebic meningoencephalitis or acanthamoebic keratitis in humans, respectively. Trophozoites encyst in unfavorable environments, such as exhausted food supply and desiccation. Until recently, the method of N. fowleri encystation used solid non-nutrient agar medium supplemented with heat-inactivated Escherichia coli; however, for the amoebic encystment of Acanthamoeba spp., a defined, slightly modified liquid media is used. In this study, in order to generate pure N. fowleri cysts, a liquid encystment medium (buffer 1) modified from Page's amoeba saline was applied for encystation of N. fowleri. N. fowleri cysts were well induced after 24 hr with the above defined liquid encystment medium (buffer 1). This was confirmed by observation of a high expression of differential mRNA of nfa1 and actin genes in trophozoites. Thus, this liquid medium can replace the earlier non-nutrient agar medium for obtaining pure N. fowleri cysts. In addition, for cyst formation of Acanthamoeba spp., buffer 2 (adjusted to pH 9.0) was the more efficient medium. To summarize, these liquid encystment media may be useful for further studies which require axenic and pure amoebic cysts.

• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.5843-5847
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• 2013

Background: Persistent infection with high risk human papillomavirus (hrHPV) is strongly associated with cervical cancer. Normal cervical cells may also harbor hrHPV, and detection of early hrHPV infection may minimize risk of cervical cancer development. This study aimed to compare two commercial HPV genotyping assays that may affordable for early screening in a limited-resource setting in Bandung, Indonesia. Materials and Methods: DNA from cervical biopsies with histologically confirmed as squamous cell cervical cacinoma were HPV genotyped by Linear Assay 1 (Roche Diagnostics, Mannheim, Germany) or Linear Assay 2 (Digene HPV Genotyping RH Test, Qiagen Gaithersburg, MD). In a subset of samples of each group, HPV genotype results were then compared. Results: Of 28 samples genotyped by linear assay 1, 22 (78.6%) demonstrated multiple infections with HPV-16 and other hrHPV types 18, 45 and/or 52. In another set of 38 samples genotyped by linear assay 2, 28 (68.4%) were mostly single infections by hrHPV type 16 or 18. Interestingly, 4 samples that had been tested by both kits showed discordant results. Conclusions: In a limited-resource area such as in Indonesia, country with a high prevalence of HPV infection a reliable cervical screening test in general population for early hrHPV detection is needed. Geographical variation in HPV genotyping result might have impacts for HPV prevalence and molecular epidemiology as the distribution in HPV genotypes should give clear information to assess the impact of HPV prophylactic vaccines.