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Breeding of Early Heading Date with High Yield Using CRISPR/Cas9 in Rice

  • Eun-Gyeong Kim;Jae-Ryoung Park;Yoon-Hee Jang;Kyung-Min Kim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.285-285
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    • 2022
  • Recent unpredictable climate change is a major cause of rice yield loss. In particular, methane is a key factor in global warming. Therefore rice breeders are trying to breed the reducing-methane gas emission rice using the crossbreeding method. However, the traditional crossbreeding method takes 8 to 10 years to breed a cultivar, and the anther culture method developed to shorten the breeding cycle also takes 6 to 7 years. On the other hand, CRISPR/Cas9 accurately edits the target trait and can rapidly breed rice cultivars by editing the target trait as a homozygous in 2-3 years. In addition, exogenous genetic elements such as Cas9 can be isolated from the G1 generation. Therefore, the flowering time was regulated by applying CRISPR/Cas9 technology, and OsCKq1 genome-editing (OsCKq1-G) rice with early flowered and high yield was bred in the field. Genome-editing of OsCKq1 applied CRISPR/Cas9 technology up-regulates the expression of the flowering promotion gene Ehd1 under long-day conditions induces early flowering and increases the yield by increasing the 1,000-grain weight. And as the generations advanced, each agricultural trait indicated a low coefficient of variation. As a result, indicated that OsCKq1 plays an important role in regulating the flowering time and is related to the trait determining yield. Therefore, OsCKq1-G can suggest a breeding strategy for the Net-Zero national policy for reducing-methane gas emission rice by shortening the breeding cycle with the early flowered, and high-yield rice. CRISPR/Cas9 technology is a rapid and accurate breeding technology for breeding rice cultivars with important characteristics.

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Temperature Effect on the Growth Parameters of Rice during Vegetative Period

  • Yin Myat Myat Min;Seo-Young Yang;Hyeon-Seok Lee;Myeong-Gu Choi;Chung-Gun Lee;Woon-Ha Hwang
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.133-133
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    • 2022
  • Temperature is a crucial environmental factor for rice cultivation due to the climate change and can influence the rice growth and development. Therefore, the effect of temperature on plant growth characters was examined during the vegetative stage. Plants were grown under three different temperatures: 23℃/13℃ for 18℃, 26℃/16℃ for 21℃ and 29℃/19℃ for 24℃ in the phytotron. The temperature was treated after transplanting and ended in early panicle initiation stage. Heading date of the two varieties were strongly affected by the temperature and were delayed in the 18℃. The plant height in the 18℃ was 21 % shorter than the 21℃ and 24℃ and the tiller and leaf number were increased in the 18℃. All the growth rates of the characters were the slowest in 18℃. The stem dry weight was significantly increased in 18℃. Nitrogen content was increased in the leaves of 18℃ whereas available phosphate and potassium content was found to be increased in the stems of 21℃ and 24℃. OsNRT 2.1 was overexpressed in the leaves and stems of 18℃ and OsNRT2.3a could be expressed in 18℃ and 21℃ temperatures whereas more expressed in 21℃. OsPT1 and OsPT6 could be expressed in the leaf of 18℃ and 24℃ but could be expressed in the stem of 18℃. OsHAK1 and OsHAK5 could be overexpressed in the leaves and stems of 18℃. For hormone, OsCKX2 gene was found to be up regulated in the leaves of 18℃ and OsIAA1 gene could be expressed more in the stem of 24℃.

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Comparison of Productivity and Feed Value of Silage Corn according to the Cutting Height

  • Yan Fen Li;Li Li Wang;Young Sang Yu;Xaysana Panyavong;Hak Jin Kim;Jong Geun Kim
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.3
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    • pp.129-137
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    • 2023
  • Corn silage is extensively utilized in ruminant feeding on a global scale, with substantial research efforts directed towards enhancing its nutritional worth and managing moisture content. The purpose of this study was to assess the impact of normal cutting height and elevated cutting height on whole-crop corn silage. Corn was harvested at heights of 15 cm and 45 cm above the ground, respectively, 45 days after heading. The harvested corn was cut into 2-3 cm lengths and packed into 20-liter plastic silos in triplicate. The results showed that dry matter (DM), crude protein (CP), water soluble carbohydrates (WSC), and in vitro dry matter digestibility (IVDMD) of C45 were significantly higher than those of the control, while the neutral detergent fiber (NDF) was significantly lower in C45 (p<0.05). The C15 had higher yields than C45 (p<0.05). There was no significant difference in the total digestible nutrients (TDN) yield of whole-crop corn silage. The increase in cutting height resulted in a larger change in moisture content and NDF per centimeter. After 60 days-ensiling, C45 showed significantly lower NH3-N concentrations. Moreover, C45 had significantly higher lactic acid concentration, lactic acid/acetic acid ratio, and lactic acid bacteria count compared to the control. Mold was not detected and the yeast count was less than 2 log10 cfu/g fresh matter in both control and C45. In summary, C45 improved the feeding value and fermentation quality of whole-crop corn silage at the expense of forage productivity.

An Outlook on Cereal Grains Production in South Korea Based on Crop Growth Simulation under the RCP8.5 Climate Change Scenarios (RCP8.5 기후조건의 작물생육모의에 근거한 우리나라 곡물생산 전망)

  • Kim, Dae-Jun;Kim, Soo-Ock;Moon, Kyung-Hwan;Yun, Jin-I.
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.14 no.3
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    • pp.132-141
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    • 2012
  • Climate change impact assessment of cereal crop production in South Korea was performed using land attributes and daily weather data at a farm scale as inputs to crop models. Farmlands in South Korea were grouped into 68 crop-simulation zone units (CZU) based on major mountains and rivers as well as existing land use information. Daily weather data at a 1-km grid spacing under the A1B- and RCP8.5 scenarios were generated stochastically to obtain decadal mean of daily data. These data were registered to the farmland grid cells and spatially averaged to represent climate conditions in each CZU. Monthly climate data for each decade in 2001~2100 were transformed to 30 sets of daily weather data for each CZU by using a stochastic weather generator. Soil data and crop management information for 68 CZU were used as inputs to the CERES-rice, CERE-barley and CROPGRO-soybean models calibrated to represent the genetic features of major domestic cultivars in South Korea. Results from the models suggested that the heading or flowering of rice, winter barley and soybean could be accelerated in the future. The grain-fill period of winter barley could be extended, resulting in much higher yield of winter barley in most CZUs than that of rice. Among the three major cereal grain crops in Korea, rice seems most vulnerable to negative impact of climate change, while little impact of climate change is expected on soybeans. Because a positive effect of climate change is projected for winter barley, policy in agricultural production should pay more attention to facilitate winter barley production as an adaptation strategy for the national food security.

Cencept of adaptability for heavy nitrogen in view of nitrogen metabolism -II. Nitrogen metabolism under the change of itrogen nenvironment (내비성(耐肥性)에 관(關)한 질소대사적(窒素代謝的) 개념(槪念) -II. 질소환경(窒素環境) 변화(變化)에 따른 질소대사(窒素代謝))

  • Yoon, Jong Hyuk;Park, Hoon;Cho, Sung Jin
    • Korean Journal of Soil Science and Fertilizer
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    • v.7 no.1
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    • pp.49-53
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    • 1974
  • Varietal difference in nitrogen metabolism was investigated under water culture system with high (50 or 80ppm) and low (10 or 40 ppm) levels of nitrogen and with two-week minus nitrogen treatment at maximum tillering and heading stage using a leading local variety, Jinheung and high-yielding IR667 line (newly bred tropical variety). 1. In high nitrogen level Jinheung showed higher yield than IR667. vise versa in low nitrogen level. 2. Poor yield of IR667 at high nitrogen may be due to ammonium toxicity that was eliminated by minus nitrogen from culture solution. 3. IR667 was more sensitive to the change of nitrogen environment. 4. With high nitrogen medium Jinheung showed nigher nitrogen uptake and higher capacity of protein synthesis than IR667, and vise versa at low nitrogen medium. 5. From the above facts it could be concluded that Jinheung has higher metabolic adaptability for heavy nitrogen while IR667 has higher structural adaptability for heavy mitrogen and that better productive adaptability will be resulted in the combination of both characteristics.

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Evaluation of Mobile Device Based Indoor Navigation System by Using Ground Truth Information from Terrestrial LiDAR

  • Wang, Ying Hsuan;Lee, Ji Sang;Kim, Sang Kyun;Sohn, Hong-Gyoo
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.36 no.5
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    • pp.395-401
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    • 2018
  • Recently, most of mobile devices are equipped with GNSS (Global Navigation Satellite System). When the GNSS signal is available, it is easy to obtain position information. However, GNSS is not suitable solution for indoor localization, since the signals are normally not reachable inside buildings. A wide varieties of technology have been developed as a solution for indoor localization such as Wi-Fi, beacons, and inertial sensor. With the increased sensor combinations in mobile devices, mobile devices also became feasible to provide a solution, which based on PDR (Pedestrian Dead Reckoning) method. In this study, we utilized the combination of three sensors equipped in mobile devices including accelerometer, digital compass, and gyroscope and applied three representative PDR methods. The proposed methods are done in three stages; step detection, step length estimation, and heading determination and the final indoor localization result was evaluated with terrestrial LiDAR (Light Detection And Ranging) data obtained in the same test site. By using terrestrial LiDAR data as reference ground truth for PDR in two differently designed experiments, the inaccuracy of PDR methods that could not be found by existing evaluation method could be revealed. The firstexperiment included extreme direction change and combined with similar pace size. Second experiment included smooth direction change and irregular step length. In using existing evaluation method which only checks traveled distance, The results of two experiments showed the mean percentage error of traveled distance estimation resulted from three different algorithms ranging from 0.028 % to 2.825% in the first experiment and 0.035% to 2.282% in second experiment, which makes it to be seen accurately estimated. However, by using the evaluation method utilizing terrestrial LiDAR data, the performance of PDR methods emerged to be inaccurate. In the firstexperiment, the RMSEs (Root Mean Square Errors) of x direction and y direction were 0.48 m and 0.41 m with combination of the best available algorithm. However, the RMSEs of x direction and y direction were 1.29 m and 3.13 m in the second experiment. The new evaluation result reveals that the PDR methods were not effective enough to find out exact pedestrian position information opposed to the result from existing evaluation method.

Variation of organic matter and rice yield in in continuous forage use of barley and rice straw

  • Ku, Bon-Il;Hwang, Jae-Bok;Choi, In-Bea;Bae, Hui-Su;Kim, Hag-Sin;Park, Tae-Seon;Park, Hong-Kyu;Lee, Geon-Hwi
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.184-184
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    • 2017
  • Organic matter is very important and essential factor to maintain productivity of paddy field. But as meat consumption and the demand of grain increase, the demand of forage also increased gradually in Korea. So the amount of organic matter in paddy field have been declined gradually by the reduction of return of rice straw for the forage use for cattle. There is not enough alternative resources for forage, we guess this trend of organic matter decline in paddy field would continue for the time being in Korea. So this study was performed to confirm the variation of organic matter and change of rice yield to select suitable rice cultivars which yield reduction is small in condition of organic matter decrease in paddy field. To confirm the change of rice growth and yield in condition of organic matter decrease in paddy field, we transplanted 10 rice cultivars which yield reduction are small in low fertilizer condition. We transplanted it Buan where double cropping of winter barley continues several years on June 10. Planting density were $70plants/3.3m^2$ Fertilizer amount was $N-P_2O_5-K_2O=9-4.5-5.7kg/10a$ and fertilizer split application of nitrogen was basal-tillering stage-panicle initiation = 50-20-30%. And in the other cultivation management, we observed rice standard culture of NICS. In paddy field where withdraw straws of barley and rice, the organic matter content showed tendency to decrease as the years go on. During rice cultivation season, organic matter decreased little by little, but it increased again after rice harvest season. Rice yield was more in order of Sodami, Chunghaejinmi and Saenuri. We judge that there is an advantage in rice yield of rice cultivars which have later heading date because of suitable ripening temperature. Although Sobibyeo and Shindongjinbyeo showed high yield, head rice yield decreased severely owing to chalky and cracked rice.

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Relationship between rice grain filling of chucheongbyeo and climatic variables of maturing Period in paddy field

  • Choi, Byoung-Rourl;Jang, Jung-Hee;Won, Tae-Jin;Lee, Jong-Hyeong;Han, Sang-Wook
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.311-311
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    • 2017
  • The relationships between rice grain filling and air temperature of maturing period in paddy field were analyzed to evaluate the effects of climatic change on rice productivity in Korea. Data of air temperature of 31, 41 and 51 days after heading(DAH) for 11 years from 2002 to 2016 were collected and analyzed to determine the effects on rice yield and yield component related traits of Chucheogbyeo, popular cultivar in Gyeonggi province in Korea. As the results, ripening ratio was closely correlated with the mean of daily maximum temperature (DMAT $r=0.71^*$), the mean of daily temperature difference (DTD, $r=0.67^*$) of 41 DAH and DTD ($0.65^*$) of 51 DAH. Weight of 1,000 paddy rice grains was closely correlated with accumulated sunshine hours (ASH) of 31 ($r=0.84^{**}$), 41 ($r=0.75^{**}$), 51 ($r=0.72^*$) DAH. Brown rice grain weight recovery ratio was closely correlated with DTD ($r=0.76^{**}$) and ASH ($r=0.84^{**}$) of 31 DAH, DMAT ($r=0.75^{**}$, $r=0.79^{**}$), DTD ($r=0.79^{**}$, $r=0.77^{**}$) and ASH ($r=0.81^{**}$, $r=0.79^{**}$) of 41 and 51 DAH. Paddy rice yield was closely correlated with MDT ($r=-0.63^*$) of 31 DAH, mean of daily minimum temperature (DMIT, $r=-0.83^{**}$, $r=-0.70^*$), DTD ($r=0.71^*$, $r=0.62^*$) of 31 and 41 DAH. Brown rice yield was correlated closely with DMIT ($r=-0.86^{**}$, $r=-0.73^*$) and DTD ($r=0.80^{**}$, $r=0.72^*$) of 31, 41 DAH, and DTD ($r=0.69^*$) of 51 DAH. Milled rice yield was correlated closely with DMIT ($r=-86^{**}$, $r=-0.73^*$), DTD ($r=0.79^{**}$, $r=0.71^*$) of 31, 41 DAH, and DTD ($r=0.68^*$) of 51 DAH.

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A Study on the First Person Narrator in Animation : Focusing on the narration of childhood experience as retrospection (애니메이션의 일인칭 서술자 연구 : 회상으로서의 유년 체험 서술을 중심으로)

  • Cho, Mi-Ra
    • Cartoon and Animation Studies
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    • s.22
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    • pp.31-45
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    • 2011
  • The paper holds its purpose to analyze the descriptive function and meaning of first person animation which the focalizer, character, and all narrators are indicated as 'I', For the purpose, the following was reviewed; the relation between 'I' as child memorizing the days of childhood as adult and the current 'I' as adult, and the aesthetic effect of experience and sense of the child on the audience reading the narration. The retrospective narrating situation of the adult narrator brings descriptive effect which comes from 'the tension between the experiencing self (self as child) and the narrative self (self as adult). The works focus on the content of child experience through the confession of the adult narrator, but the view of the adult always heading towards 'the present'. That is, the aesthetics contained by the first person narrator is related to endless arousal of the values of hidden and forgotten things. In addition, the descriptive method of child focalizer as 'the subject of experience' brings qualitative change which enables reasoning of the subject as itself, which is free from the view tamed by rational system. Becoming an adult, the lost ability of mimesis brings qualitative change by meeting with the generality of childhood sense. Therefore, it can be known that the meaning the narrator contains in the first person narrator condition of animation links with the degree of aesthetic completion of the work, but also, it is a highly strategic descriptive device which determinately affects even the acceptance of audiences regarding the work.

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Study on the Quality of Whole Silage and Yield by Stage of Maturity of Rye Plant I. Change of composition and yield by stage of maturity of whole crop rye plant (호맥의 생육시기별 수량과 Whole crop silage의 품질에 관한 연구 I. 호맥의 생육시기별 수량 및 성분변화)

  • 고영두;문영식;곽종형
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.6 no.1
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    • pp.19-23
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    • 1986
  • This experiment was conducted to find out the change of composition and yield of whole crop rye plant by the maturity stage such as boot, heading, milky, dough and yellow ripe. The results of this experiment were as follows: The yield of whole plant was significantly (P<0.01) higher in the dough stage than in the other stages, and stalk yield of whole plant was significantly (P<0.01) higher in the dough stage than in the other stages, and stalk yield of whole plant was the highest in the part of whole plant, ear yield in the yellow ripe stage. Leaf and stalk yield in the ratio of part yield to whole plant yield remarkably decreased according to the passage from boot to yellow ripe stage, but ear yield increased. Dry matter yield increased with advancing maturity stage, and was the highest in the yellow ripe stage (P<0.01). Crude protein contents of whole plant and each parts gradually decreased, and crude fiber content increased with advancing maturity stage.

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