• The Korean Journal of Malacology
• /
• v.28 no.1
• /
• pp.37-43
• /
• 2012

In this study, we invesgated a cytogenetic analysis of the Pacific triploid abalone, Haliotis discus hannai induced by low temperature treatment. We got a lot of mitotic metaphase chromosome spreads from the triploid and diploid Pacific abalones' hatched larvae (trochophores). The chromosome number of diploid abalone was 2n = 36 and that of triploid abalone was 3n = 54, so the chromosome number of triploid abalone was 1.5 times higher than that of diploid abalone. We developed a modified flow cytometric method for Pacific abalone from the existing methods. We uesd 51 months aged triploid and diploid Pacific abalones' hemolymph to get the DNA contents by flow cytometry. The DNA content of diploid abalone was 1.743 pg/cell and the DNA content of triploid abalone was 1.49 times higher than that of diploid one. It proved that triploid abalone was consisted with two sets of maternal diploid and one set of paternal genome.

• The Korean Journal of Malacology
• /
• v.32 no.4
• /
• pp.241-247
• /
• 2016

Macrophage Migration Inhibitory Factor (MIF) are well-defined role as unique cytokine and critical mediator in acute and chronic inflammatory diseases, autoimmune diseases. In this study, we isolated and characterized a full-length of MIF cDNA from the abalone (Haliotis discus hannai). The full-length cDNA of abMIF was of 1264 bp, consisting of a 5'-terminal UTR of 143 bp, an open reading frame of 360 bp and a 3-terminal UTR of 761 bp. The abalone MIF cDNA encodes a 119-amino acid polypeptide with a calculated molecular mass of 13.4 kDa and isoelectric point of 9.07. Multiple alignments and phylogenetic analysis with the deduced abalone MIF protein and showed strong homology with disk abalone (Haliotis discusdiscus). The deduced amino acid sequence of abMIF exhibited homology with other reported MIFs, such as 80%, with that of other disk abalone H. discus discus MIF gene. Quantitative real-time PCR (qRT-PCR) analysis indicated that abMIF was highly expression observed in hapatopacreas, intestine, foot, and gonad of normal conditioned abalone. Even though AbMIF mRNA level in hemocytes was low under the normal condition, it was sharply up-regulated and reached the maximum at 6 h post-infection with Vibrio parahaemolyticus, and then decreased at 24 h post-infection. This result indicates that abMIF plays an important role in responding in the innate immune system.

• Proceedings of the Korean Society of Fisheries Technology Conference
• /
• 2001.05a
• /
• pp.622-623
• /
• 2001

전복류는 세계적으로 100여종으로 알려져 있고, 이중 어획 대상이 되는 대형종은 20여종으로서 주로 온대수역에서 많이 생산되고 있다. 우리나라에 서식하는 전복류는 소형종인 오분자기(Haliotis diversicolor supertexta)를 비롯하여 난류계의 대형종인 말전복(H. gigantea), 까막전복(H. discus), 시볼트 전복(H. sieboldii) 그리고, 한류계인 참전복(H. discus hannai)의 5종이 알려져 있다. (중략)

• Journal of Aquaculture
• /
• v.12 no.4
• /
• pp.317-322
• /
• 1999

A growth trial was conducted to investigate the utilization of wheat germ meal as a protein source of formulated diet for juvenile abalone (Haliotis discus hannai). Four replicate groups of the abalone average weighing 150mg were fed one of four isonitrogenous (33%) and isolipidic (6%) diets containing 0%, 10%, 20%, or 30% wheat germ meal for 18 weeks. In addition, these formulated diets were compared with commercial diet. Survival rate, weight gain, soft body weight , and shell growth of abalone fed diets containing 0%, 10%, 20%, or 30% wheat germ meal were not different (P>0.05) from those of abalonn fed the control diet and commercial diet. There were no significant differences (P>0.05) in soft body composition of moisture, protein and lipid. It si concluded that wheat germ meal were be used as a partial protein source of formulated diet for juvenile abalone.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.47 no.2
• /
• pp.154-159
• /
• 2014

The increasing of atmospheric $CO_2$ are changing the pH (ocean acidification) and temperature of the sea. Although the effects of ocean acidification on calcifying organisms have well-documented, only a few studies have examined the combined effects of ocean acidification and elevated temperature. This study investigated the effects of ocean acidification and elevated temperature for 2100 on survival and growth of juvenile abalone, Haliotis discus hannai. Ocean acidification was simulated by bubbling $CO_2$ into seawater at concentrations of 1,000 and 1,500 ppm, and temperature was set at room temperature $+2^{\circ}C$. Neither $CO_2$ nor temperature had a significant effect on survival of abalone, while both significantly affected growth. There was no significant interaction between the two factors. Shell length can be used as a growth index of abalone to access the impacts of ocean acidification and elevated temperature.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.47 no.6
• /
• pp.796-800
• /
• 2014

A feeding trial was conducted to investigate the growth and body composition of juvenile sea cucumber Apostichopus japonicus in integrated culture with abalone Haliotis discus hannai or rockfish Sebastes schlegeli. Triplicate groups of sea cucumber averaging $1.2{\pm}0.05g$ were cultured alone or with abalone or rockfish for 12 weeks. Survival of sea cucumber was not affected by co-culturing (P>0.05). Weight gain of sea cucumber cultured with rockfish was significantly higher than that of sea cucumber cultured alone (P<0.05), and did not differ from that of those cultured with abalone (P>0.05). These findings indicate that co-culturing sea cucumber with rockfish effectively improves the growth of sea cucumber.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.36 no.6
• /
• pp.601-605
• /
• 2003

This study investigated the effect of dietary pigment sources on growth and shell color of juvenile abalone(Haliotis discus hannai). Three replicate groups of the abalone (average weight 173 mg) were fed diets containing various pigment sources such as Porphyra powder, Spirulina, yeast astaxanthin, and paprika extract for 16 weeks. Survival and weight gain were not affected by dietary pigment sources (P>0.05). Shell color of abalone fed diets containing Porphyra powder and Spirulina approached the yellow-red and orange, colors similar to wild abalone. However, shell color of abalone fed the diets containing yeast astaxanthin and paprika extract were similar to the bright green control group. These results should be useful for changing the shell color of abalone in aquaculture.

• Fisheries and Aquatic Sciences
• /
• v.17 no.3
• /
• pp.385-390
• /
• 2014

Abalone immortal cell lines can be used to study the physiological properties and disease mechanisms of abalone at the cellular and molecular level. As a first step for the final goal to establish abalone immortal cell lines, we examined various initial culture conditions for primary cell populations derived from Haliotis discus hannai radula tissue. The survival rate after cell isolation procedures using the enzymatic method was as low as $9.95{\pm}2.37%$. Based on three different experimental conditions for H. discus hannai radula-derived cell culture, we found that the salinity of the media and the presence of growth-promoting factors were important to support radula-derived primary cell populations during the initial culture. The growth factor-containing media adjusted to 35 psu salinity could induce 100% (8 out of 8 trials) initial cell attachment, and the rate of cell attachment reached 50-70%. The data obtained from this study will provide useful information for developing immortal cell lines from abalone species.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.37 no.4
• /
• pp.287-294
• /
• 2004

Effect of growth and survival rate on different densities in the cage culture of juvenile abalone (Haliotis discus hannai) were determined in Myoduri Yeosu, Jeollanamdo from April 2000 to April 2001. The shell length growth was conducted using $32.35{\pm}1.35$ mm abalone juveniles for 374 days at densities $15{\%}\;(230\;indv./m^{2}),\;30{\%}\;(460\;indv./m^{2}),\;45{\%}\;(690 \;indv./m^{2}),\;60{\%}\;(920\;indv./m^{2})$. The result showed that the hightest growth rate was observed in lowest $(15{\%})$ density experimental group. Survival rate of Juvenile abalone was the highest in $15{\%}$\; density group and lowest in $45{\%}$ density group and distribution rate of shell length showed the highest as $30{\%}\;in\;230\;indv./m^{2}\;(15{\%})$ group.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.53 no.1
• /
• pp.90-97
• /
• 2020

The purpose of this study was to provide basic information on sexual maturity and reproductive biology for the management of biological resources in abalone Haliotis discus hannai. The nucleus of the oogonium occupied about 42% of the cytoplasm, and had a distinctive basophilic chromatin. The cytoplasm of previtellogenic oocytes was homogeneous and the size of nuclear pores increased. Fine granular and vacuolar yolk granules were observed in the cytoplasm of the initial vitellogenic oocyte. In this stage, the egg stalk and jelly membrane began to develop. The nucleus of the active vitellogenic oocyte was located near the animal pole. Yolk granules were strongly acidophilic. Lampbrush chromosomes were observed in the nucleus and rough endoplasmic reticulum. Annulate lamellae developed in the cytoplasm. The shape of the ripe oocyte was rounded polygonal. The size of ripe oocytes was 202.9±21.40×142.1±18.82 ㎛ and the thickness of the jelly membrane was 10.1±1.52 ㎛. These results show that yolk accumulation in H. discus hannai is based on two methods: exogenous accumulation, through the egg stalk, and endogenous accumulation, through intracellular organelles. Management of biological resources will be necessary when oocytes predominate after the active vitellogenic stage.