• The Korean Journal of Malacology
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• v.20 no.1
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• pp.17-26
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• 2004

In present study, attempts were made to preserve abalone (Haliotis discus hannai) sperm in liquid form at low temperature, to evaluate the effect of various diluents in short-term storage on sperm, and cryopreservation procedures were optimized for the cryoprotectants and freezing rates, as well as the motility, survival rate, and the ultrastructural changes of sperm after short-term storage and cryopreservation were observed. The abalone sperm reached maximum motility until about 4 min after activation. The motility was constant for about 16 min, after which it dropped gradually, and about 50 min later all motility ceased. In Hanks' balanced salt solution (HBSS, 300 and 400 mOsmol/kg) and 150, 250 and 350 mOsmol/k artificial seawater (ASW), the sperm was immotile. After 100% ASW was added, motility of those sperm, which are in 300, 400 mOsmol/kg HBSS, 250, 350 mOsmol/kg ASW, could be again restored incompletely. Sperm motility can be maintained for 20 days of cold storage only in ASW of 850 and 1200 mOsmol/kg. A high motility index of 3.5-4.5 was observed for the first 8 days in 850 and 950 mOsmol/kg ASW. In other diluents sperm motility was constant less than 10 days, and the motility index was obviously lower than that of sperm in 850 and 1200 mOsmol/kg ASW. After 20 days of cold storage, survival rates of 10.2%-20.7% were obtained in ASW and 300 mOsmol/kg HBSS, and that in 400 HBSS (65.3%) was significantly higher than others. The constant period of sperm motility stored in 850 mOsmol/kg ASW was obviously longer than that in 1200 mOsmol/kg ASW after 6 days of storage. The sperm plunged into liquid nitrogen all died except that sperm using 15% glycerol as cryoprotectant restored 10.4% of motility. The highest motility index (3.4) was obtained with 5% glycerol and freezing procedure: $-50^{\circ}C$/min from $20^{\circ}C$ to $-80^{\circ}C$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.6
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• pp.591-595
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• 2003

Settlement of larvae, growth, and survival of juvenile abalone (Haliotis discus hannai) were examined after feding of 5 species of benthic diatoms (Cocconeis suctellum, Navicula sp Nitzschia longissima Bacillavia paxillifera, Licmophora flabellata). All of the benthic diatoms were isolated from natural populations on plastic plates for the abalone. The settlement of larvae, shell growth, and survival rate of juvenile abalone varied by diatoms species. The highest settlement rate of larvae was $43.1\%$ with the Cocconeis suctellum diet. Daliy growth rate reached a maximum at 64.1 ${\mu}m/day$ with the Navicula sp. diet and a minimum at 22.4 ${\mu}m/day$ with the Licmophora flabellata diet. Survival rate of the juvenile abalone was highest at $62.0\%$ with the Navicula sp., Cocconeis suctellum , and Nitzschia longissima diets. Survival rate of the Juvenile abalone was significantly higher than the control group with mixed diatom population diets (P<0.05). Therefore, diatom . species composition in diets can be a controling factor for the settlement, growth, and survival rates of Juvenile abalone.

• The Journal of Fisheries Business Administration
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• v.46 no.1
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• pp.1-13
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• 2015

This study is aimed comparative analysis for business performance of land-based ezo abalone (Haliotis discus hannai) seed farms by standardizing cost structures by region and farming size. The result of survey on average farming incomes by region showed that farming incomes in Haenam and Jindo regions were much higher than those in other regions. Followed by Wando region, incomes in other regions were analyzed to be relatively lower. It is shown that farming incomes became higher as farm size increased. Farming incomes per unit size which were modified from farming incomes by region were highest in Jindo region, followed by East sea region, Wando and Haenam regions. Incomes in Jeju and Yeosu regions were analyzed to be relatively lower. Results on farming incomes per unit size ($m^2$) showed that both farming incomes and profits became higher as size increased. It implies that a scale effectiveness might exist in case of land-based abalone culture system. Impacts of major factors on farming profits and returns on investment in abalone seed aquaculture are summarized as follow. First, only if the survival rate increased by 10% with improvement of component ratio, variable effects became largest. In variable effects of other factors, a variation in Jindo region was largest and on the other hand, a variation in Yeosu region was shown to be smallest.

• The Korean Journal of Malacology
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• v.32 no.4
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• pp.249-254
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• 2016

The purpose of this study is to estimate genetic parameter and growth traits by sex of Pacific abalone, Haliotis discus hannai. The abalone 10 family produced using 1:1 mating system of male and female for analyses of sex ratio and growth traits (shell length, shell width, and total weight) by sex. Overall mean in phenotypic traits at 30-month-old showed 85.01 mm of shell length, 57.49 mm of shell width and 73.34 g of total weight respectively. The sex ratio (female : male) was 1:0.93 (n = 191:177). The values of growth traits by sex showed significant difference (P < 0.05). The each value of female growth traits were shown to be higher than the values of male growth traits. The heritability of growth traits by sex were estimated that the heritability of female growth traits are higher than male that. The results suggest a possibility of improving the growth of cultured abalone using selection breeding by sex.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.4
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• pp.391-394
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• 1999

A feeding experiment was conducted using juvenile abalone (Haliotis discus hannai) to investigate the optimum level of supplemental vitamin and/or mineral premixes in a formulated diet for practical feed formulation. Three replicate groups of abalone averaging 100 mg were fed the diets containing different levels of vitamin premix ($0\%,\;0.5\%,\;1.0\%$ and $2\%$) and /or mineral premix($0\%,\;2\%,\;4\%$ and $6\%$) for 4 months. Survival rates of abalone showed no distinctive trend by the levels of dietary vitamin premixes. Weight gain, shell growth and soft body weight of abalone were not significantly affected by the different dietary vitamin and/or mineral premixes (P>0,05). Moisture, protein and lipid contents of soft body were influenced by experimental diets (P<0.05) but these differences also had no definite trends by levels of vitamin and/or mineral premixes. These data indicate that supplemental vitamin or mineral premix is not needed in this formulated diet, therefore price of diet for juvenile abalone could be reduced.

• Microbiology and Biotechnology Letters
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• v.50 no.1
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• pp.22-30
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• 2022

Haliotis discus hannai called abalone, is the valuable marine mollusks and the by-products of abalone processing are viscera. Brownish abalone male viscera (AMV), which have not been reported as having anti-inflammatory effects, was extracted with acetone and fractionated by different six acetone/hexane ratios (0, 10, 20, 30, 40, and 100%) using a silica column via in vitro ABTS and DPPH radical and nitric oxide (NO) production assay-guided fractionation. Among the fractions, the acetone/hexane ratio 40%, A40 exhibited the most potent radical scavenging activities and inhibition of lipopolysaccharide (LPS)-induced NO production without cytotoxicity. A40 inhibited LPS-induced intracellular reactive oxygen species (ROS) production in a dose-dependent manner. Western blot analysis revealed that A40 down-regulated the activation of NF-κB, MAPK (ERK 1/2, p-38, and JNK), and inflammatory enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2. Moreover, this fraction inhibited the generation of pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. These results suggested that AMV containing A40 with anti-inflammatory and anti-oxidantive effects, is the effective therapeutic and functional material for treating inflammatory disorders.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.4
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• pp.541-548
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• 2023

Behavior serves as an important indicator of the effects of environmental stressors on organisms. In this study, one-year-old abalone Haliotis discus hannai (15.1±0.8 mm) were used and each 0.34 m2 tank was stocked with 100, 150, 200, 250, and 300 juvenile abalone. After 328 days, we found that the low stocking density treatment showed significantly higher growth, whereas the high stocking density treatment showed significantly lower growth. In addition, abalone showed a lower rate of movement, distance moved, and velocity at higher stocking densities, but only velocity differed significantly. In contrast, In the Supplied the feed tanks significant difference between the moving distance and velocity. When the two groups of different sizes were mixed in tank, the rate of movement, distance moned, and velocity showed significant differences between differently-sized indivisuals. Our study indicates that breeding abalone at high densities has a negative effect on their growth and survival, which is thought to primarily be caused by spatial competition, and secondarily by differences in body size, ultimately affecting growth and productivity.

• Fisheries and Aquatic Sciences
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• v.26 no.5
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• pp.355-365
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• 2023

The Pacific abalone, Haliotis discus hannai, is a highly valued and industrially important aquaculture species with growing demands of the expanding abalone aquaculture industry. To explore the feasibility of using the brown alga, Sargassum horneri, as a potential substitute for abalone feed, it is important to identify the feed preference and attractant effect of S. horneri on Pacific abalone. Our experiments indicated that the feeding-associated movement of abalone could be detected using a video tracking system under indirect illumination with dim red light. To further analyze the attraction potentials of various test materials, preference analysis was performed using Avicel-coated glass plates with ground powders of various seaweeds (e.g., S. horneri, Saccharina japonica, and Undaria pinnatifida) and commercial abalone feed, together with coffee waste. Heat map analysis indicated greater attraction by the kelp S. japonica than by S. horneri and commercial feed, which showed similar preference levels. Feeding preference based on the area of Avicel eaten by abalone showed a significant preference for U. pinnatifida over S. horneri (feeding area: 68.6 ± 20.1% vs. 37.5 ± 22.4%, p < 0.05). Additionally, the feeding area was significantly greater for plates with S. japonica than for plates with S. horneri (44.0 ± 16.6% vs. 22.6 ± 15.4%, p < 0.05). There was no significant difference in feeding area between commercial feed and S. horneri (31.7 ± 11.6% vs. 31.6 ± 20.2%, p > 0.05). The methanol extracts attracted abalone in the following order: U. pinnatifida > S. horneri > S. japonica > commercial feed > coffee waste. To determine the attractive effects of the components of methanol extracts, mixtures of methanol extracts of commercial feed with increasing amounts of S. horneri were examined. The results showed a significant increase in feeding preference upon addition of S. horneri up to 50% and 75%, suggesting its potential for use as an appetite-enhancing feed additive. This study identified conditions that can be successfully used to monitor the movement of Pacific abalone; the results of preference analysis confirmed that abalone exhibited similar attraction and feeding preference for S. horneri, compared with commercial feed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.57 no.3
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• pp.203-208
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• 2024

Fifteen cultured frozen abalone Haliotis discus hannai samples were purchased from supermarkets, traditional markets, online markets, and processing factories throughout Korea for the safety assessment of microbiological and chemical hazards. Sanitary-indicative (total viable bacteria, coliforms, and Escherichia coli) and pathogenic (Staphylococcus aureus, Salmonella spp., Listeria monocytogenes, Vibrio parahaemolyticus, Clostridium perfringens, and Enterohemorrhagic E. coli) bacterial contamination levels were assessed quantitatively or qualitatively. Additionally, heavy metal content (lead, cadmium, and total mercury) and radioactivity (¹³⁴CS+¹³⁷CS, ¹³¹I) were quantitatively assessed. The total viable bacterial count was 4.3×10² CFU/g, while coliform count was 50 CFU/g. E. coli was not detected in any of the samples (count < 10 CFU/g). All six pathogenic bacteria tested negative qualitatively. The average lead, cadmium, and total mercury contamination levels in the cultured frozen abalone were 0.100±0.057, 0.145±0.061, and 0.015±0.001 mg/kg, respectively. Moreover, none of the samples were radioactive. According to the results of this study, cultured frozen abalones distributed in all types of markets were safe from all microbiological and chemical hazards.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.57 no.4
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• pp.349-354
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• 2024

This study investigates the effectd of substituting Undaria pinnatifida with a laver Porphyra tenera by-product (LB) on the growth performance, and soft body composition of juvenile abalone Haliotis discus hannai. A total of 750 juvenile abalones were evenly distributed across 15 tanks with 90 individuals per tank. Five diets were formulated; a control diet (Con) containing 30% U. pinnatifida and four experimental diets in which 25%, 50%, 75%, and 100% U. pinnatifida were substituted with equal amounts of LB (LB25, LB50, LB75, and LB100, respectively). The feeding trial lasted for 6 weeks, with daily feeding at 16:00. Survival rate, growth performance (weight gain and specific growth rate), and soft body composition of the abalones were not affected by the substitution levels (P>0.05). Therefore, this study concluded that complete replacement of LB with 100% U. pinnatifida in the abalone diet did not adversely affect survival, growth performance, or soft body composition.