• Molecules and Cells
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• v.21 no.1
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• pp.29-33
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• 2006

We have established three immortal bovine muscular epithelial (BME) cell lines, one spontaneously immortalized (BMES), the second SV40LT-mediated (BMEV) and the third hTERT-mediated (BMET). The morphology of the three immortal cell lines was similar to that of early passage primary BME cells. Each of the immortal cell lines made cytokeratin, a typical epithelial marker. BMET grew faster than the other immortal lines and the BME cells, in 10% FBS-DMEM medium, whereas neither the primary cells nor the three immortal cell lines grew in 0.5% FBS-DMEM. The primary BME cells and the immortal cell lines, with the exception of BMES, made increasing amounts of p53 protein when treated with doxorubicin, a DNA damaging agent. On the other hand, almost half of the cells in populations of the three immortal cell lines may lack $p16^{INK4a}$ regulatory function, compared to primary BME cells that were growth arrested by enforced expression of $p16^{INK4a}$. In soft-agar assays, the primary cells and immortal cell lines proved to be less transformed in phenotype than HeLa cells. The three immortal epithelial-type cell lines reported here are the first cell lines established from muscle tissue of bovine or other species.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.31 no.1C
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• pp.29-38
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• 2011

The smaller diameter cone penetrometer has been widely used to estimate the characteristics of local area due to high vertical resolution. The half-bridge cirucits have been adopted to measure the mechnical strength of soil through the smaller diameter cone penetrometer due to the limitation of the areas for configuring the full-bridge circuit. The half-bridge circuit, however, is known as being easily affected to the temperature variation. The objective of this study suggests the temperature-compensated method in mini-cones. The diameter and length of the mini-cone is designed to 15mm and 56mm. The load cell of the mini-cone is extended about 54mm on the behind of the mini-cone to reflect the only temperature variation. The full-bridge circuit is installed to measure the temperature-compensated values in the mini-cone and the half-bridge circuit is also organized to compare the temperature compensated values with uncompensated values. The seasonal variation tests are performed to define the effect of temperature variation under summer and winter temperature condition. The densification tests are also carried out to investigate temperature effects during penetration. The measured mechanical resistances with temperature-compensated method show more reliable and reasonable values than those measured by thermal uncompensated system. This study suggests that the temperature-compensated method of the mini-cone may be a useful technique to obtain the more reliable resistances with minimizing the temperature effect.

• Proceedings of the Materials Research Society of Korea Conference
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• 2011.05a
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• pp.5-5
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• 2011

The research and development of hybrid electric vehicle (HEV), plug-in hybrid electric vehicle (PHEV) and electric vehicle (EV) are intensified due to the energy crisis and environmental concerns. In order to meet the challenging requirements of powering HEV, PHEV and EV, the current lithium battery technology needs to be significantly improved in terms of the cost, safety, power and energy density, as well as the calendar and cycle life. One new technology being developed is the utilization of composite cathode by mixing two different types of insertion compounds [e.g., spinel $LiMn_2O_4$ and layered $LiMO_2$ (M=Ni, Co, and Mn)]. Recently, some studies on mixing two different types of cathode materials to make a composite cathode have been reported, which were aimed at reducing cost and improving self-discharge. Numata et al. reported that when stored in a sealed can together with electrolyte at $80^{\circ}C$ for 10 days, the concentrations of both HF and $Mn^{2+}$ were lower in the can containing $LiMn_2O_4$ blended with $LiNi_{0.8}Co_{0.2}O_2$ than that containing $LiMn_2O_4$ only. That reports clearly showed that this blending technique can prevent the decline in capacity caused by cycling or storage at elevated temperatures. However, not much work has been reported on the charge-discharge characteristics and related structural phase transitions for these composite cathodes. In this presentation, we will report our in situ x-ray diffraction studies on this mixed composite cathode material during charge-discharge cycling. The mixed cathodes were incorporated into in situ XRD cells with a Li foil anode, a Celgard separator, and a 1M $LiPF_6$ electrolyte in a 1 : 1 EC : DMC solvent (LP 30 from EM Industries, Inc.). For in situ XRD cell, Mylar windows were used as has been described in detail elsewhere. All of these in situ XRD spectra were collected on beam line X18A at National Synchrotron Light Source (NSLS) at Brookhaven National Laboratory using two different detectors. One is a conventional scintillation detector with data collection at 0.02 degree in two theta angle for each step. The other is a wide angle position sensitive detector (PSD). The wavelengths used were 1.1950 ${\AA}$ for the scintillation detector and 0.9999 A for the PSD. The newly installed PSD at beam line X18A of NSLS can collect XRD patterns as short as a few minutes covering $90^{\circ}$ of two theta angles simultaneously with good signal to noise ratio. It significantly reduced the data collection time for each scan, giving us a great advantage in studying the phase transition in real time. The two theta angles of all the XRD spectra presented in this paper have been recalculated and converted to corresponding angles for ${\lambda}=1.54\;{\AA}$, which is the wavelength of conventional x-ray tube source with Cu-$k{\alpha}$ radiation, for easy comparison with data in other literatures. The structural changes of the composite cathode made by mixing spinel $LiMn_2O_4$ and layered $Li-Ni_{1/3}Co_{1/3}Mn_{1/3}O_2$ in 1 : 1 wt% in both Li-half and Li-ion cells during charge/discharge are studied by in situ XRD. During the first charge up to ~5.2 V vs. $Li/Li^+$, the in situ XRD spectra for the composite cathode in the Li-half cell track the structural changes of each component. At the early stage of charge, the lithium extraction takes place in the $LiNi_{1/3}Co_{1/3}Mn_{1/3}O_2$ component only. When the cell voltage reaches at ~4.0 V vs. $Li/Li^+$, lithium extraction from the spinel $LiMn_2O_4$ component starts and becomes the major contributor for the cell capacity due to the higher rate capability of $LiMn_2O_4$. When the voltage passed 4.3 V, the major structural changes are from the $LiNi_{1/3}Co_{1/3}Mn_{1/3}O_2$ component, while the $LiMn_2O_4$ component is almost unchanged. In the Li-ion cell using a MCMB anode and a composite cathode cycled between 2.5 V and 4.2 V, the structural changes are dominated by the spinel $LiMn_2O_4$ component, with much less changes in the layered $LiNi_{1/3}Co_{1/3}Mn_{1/3}O_2$ component, comparing with the Li-half cell results. These results give us valuable information about the structural changes relating to the contributions of each individual component to the cell capacity at certain charge/discharge state, which are helpful in designing and optimizing the composite cathode using spinel- and layered-type materials for Li-ion battery research. More detailed discussion will be presented at the meeting.

• Proceedings of the KIEE Conference
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• 2006.04b
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• pp.362-364
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• 2006

This paper presents a novel DC-DC Converter for 3 [kW] Fuel Cell System Residential Application. Phase shifted control is employed to regulate the output voltage and achieve soft switching. The transformer leakage inductance is utilized effectively to achieve zero voltage turn on for the power semiconductor switches. The current doubler rectifier has only one diode drop. The transformer secondary winding current rating is one half the load current. The overall effciency of the converter is improved.

• Proceedings of the KIEE Conference
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• 1991.07a
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• pp.846-849
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• 1991

This paper reports the principle, system confiquration, test results of optical voltage sensor using quartz pockels cell. The Pockels effect of quartz material is used for designing optical voltage sensor. The quarts material has very high half-wave voltage, so, it can be applied to measure high voltage level. Experimental results show that the optical voltage sensor has excellent linear characteristics within the applied AC voltage of 1200V.

• Proceedings of the KIEE Conference
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• 2006.10d
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• pp.132-134
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• 2006

This paper presents the design and implementation of 1 kW prototype DC-DC power converter with bridge voltage-doubler suitable for small scale PEM fuel cell system and its associated control scheme. The operation principle of this converter is described using some operating waveforms and the switching mode equivalent circuits based on simulation results and a detailed circuit operation analysis and soft-switching conditions.

• Journal of electromagnetic engineering and science
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• v.18 no.3
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• pp.182-187
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• 2018

A low profile asymmetrical fractal boundary patch antenna based on reactive impedance surface (RIS) and a mushroom unit cell (MUC) is proposed and studied for dual band operation. The sides of the square patch antenna are replaced with asymmetrical half circled fractal curves for circular polarization operation at patch mode band. The fractal patch antenna is loaded with MUC for dual band operation. The antenna radiation characteristics are investigated and illustrated with both simulated and experimental results in detail. The 10-dB return loss bandwidth are 8.48% (3.21-3.49 GHz) and 2.59% (2.30-2.36 GHz) at upper and lower resonance frequencies, respectively. The 3-dB axial ratio bandwidth is 4.26% (3.21-3.35 GHz). A close agreement between simulation data with experimental results is observed.

• Journal of Ocean Engineering and Technology
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• v.10 no.4
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• pp.103-108
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• 1996

To syudy the change of prtential in the mortar-embedded precorroded rebar, a half cell method was adapted. The rebar was corroded by the salt spray and then the rebar embedded in the mortar. A saturated copper sulfate feference electrode was used. The corrosion potential of the rebar in the mortar specimen cured in air was increased, whereas that of the rebar cured in water was decreased with aging. The corrosion potential of the rebar in the mortar was decreased with corroded time by the salt spray. As the mortar thickness covered the rebar was increased, the corrosion potential of the rebar in the mortar was increased.

• Journal of the Korean Ceramic Society
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• v.37 no.2
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• pp.128-133
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• 2000

In this work, soft carbons produced by pyrolysis of petroleum and coal-tar pitch were used as the negative electrode for Li-ion batteries. We studeid the charge/discharge capacity and the interfacial reaction of these electrodes by constructing a half cell. Charge/discharge property was studied by a constant-current step and the interfacial reaction between the electrolyte and the surface of a carbon electrode was studied by the cyclic voltammetry. The initial charge/discharge capacity for the coal-tar pitch carbon increased exceedingly with the heat treatment temperature. On hte other hand, the capacity of the petroleum pitch carbon increased with temperature up to 1000$^{\circ}C$, thereafter decreased continuously. While the charge capacity decreased with the cycle number, the reversibility increased above 90%. In addition, the thermal stability and crystallization of petroleum and coal-tar pitches were analyzed by TGA and XRD, respectively.

• Journal of Microbiology and Biotechnology
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• v.3 no.4
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• pp.244-251
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• 1993

A novel system has been developed to produce ${\delta}-aminolevulinic$ acid (ALA) using the intact cells of late logarithmic phase of Rhodocyclus gelatinosus KUP-74. The system was shown optimum yield of extracellular ALA under a condition of anaerobic light irradiation (4 Klux) at $30^{\circ}C$ with no variation in cell mass. The rate of extracellular ALA formation was stimulated by low doses of either $C_4\;or\;C_5$ ALA biosynthetic precursors, where 5 mM ($C_4) and 3 mM ($C_5) of each precursors were appeared to generate the maximum rates of 3.3 and 4.0 nmoles of ALA per 0.35 mg cells per hr, respectively. Half-life of the system was 10 hr in a sense of an ability of portage transport of L-glutamate, and sequential dose of this compound was resulted in promising recovery of the ALA.