• Journal of Wetlands Research
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• v.1 no.1
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• pp.29-44
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• 1999

Vegetation structure and distribution of the vascular hydrophytes and hygrophytes, and the growth pattern, standing crop and amounts of nutrient uptake by Salix species were investigated in the upper stream wetlands of the Namgang-Dam, Chinju-city, Gyeongsangnam-do, Korea from April to November in 1997. The flora was composed of 43 hydrophytes and 241 hygrophytes, or total 284 vascular plants. The life forms of the hydrophytes were classified as 27 kinds of emergent plants, 4 floating-leaved plants, 3 free-floating plants, and 9 submersed plants. In the herb layer, the dominant species was Persicaria hyciropiper, and the ranges of the species diversity indices (H'), equitabilities, (J') and community similarity indices (CCs) were 1.59~1.89, 0.87~0.96, and 0.35~0.83, respectively. In the shrub and subtree layers, 17 kinds of Salix species were supposed to the pioneer plants at the early stage of the succession. The number of branches per main stem of Salix species was 5.0. The DBH class-frequency histograms of Salix species were the reverse J type, and the natural regeneration of the Salix community was expected. Basal area of Salix species per square meter was $24.87cm^2$. Volume of Salix species per square meter was $12,008cm^3$ and total phytomass of the Salix species was estimated as 12,894 ton. Biomass distribution of Salix species in the stem, the branch and twig, and the leaf was 64.1%, 28.1%, and 7.8%, respectively. The amounts of nitrogen and phosphorus absorbed by Salix species were 68,022 and 19,823 kg. It was recommended that application and conservation of the wetland and other counterplans are indispensable to reduce the adverse effects of water pollution and to preserve the wetland ecosystem.

• Journal of Life Science
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• v.17 no.10
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• pp.1447-1451
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• 2007

Through the screening of marine natural compounds that inhibit cancer cell proliferation, we previously reported that pectenotoxin-2 (PTX-2) isolated from marine sponges exhibits selective cytotoxicity against several cell lines in p53-deficient tumor cells compared to those with functional p53. However, the molecular mechanisms of its anti-proliferative action on malignant cell growth are not completely known. To further explore the mechanisms of its anti-cancer activity and to test whether the status of p53 in liver cancer cells correlates with their chemo-sensitivities to PTX-2, we used two well-known hepatocarcinoma cell lines, p53-deficient Hep3B and p53-wild type HepG2. We have demonstrated that PTX-2 markedly inhibits Hep3B cell growth and induces apoptosis whereas HepG2 cells are much more resistant to PTX-2 suggesting that PTX-2 seems to act by p53-independent cytotoxic mechanism. The apoptosis induced by PTX-2 in Hep3B cells was associated with the modulation of DNA fragmentation factor (DFF) family proteins, up-regulation of pro-apoptotic Bcl-2 family members such as Bax and Bcl-xS and activation of caspases (caspase-3, -8 and -9). Blockade of the caspase-3 activity by caspase-3 inhibitor, z-DEVD-fmk, prevented the PTX-2-induced growth inhibition in Hep3B cells. Moreover, treatment with PTX-2 also induced phosphorylation of AKT and extracellular-signal regulating kinase (ERK), but not c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MARK). Specific inhibitors of PI3K inhibitor (LY294002) and ERK1/2 inhibitor (PD98059) significantly blocks PTX-2-induced-anti-proliferative effects, whereas a JNK inhibitor (SP600125) and a p38 MAPK inhibitor (SB203580) have no significant effects demonstrating that the pro-apoptotic effect of PTX-2 mediated through activation of AKT and ERK signal pathway in Hep3B cells.

• Restorative Dentistry and Endodontics
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• v.31 no.4
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• pp.312-322
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• 2006

The objectives of this study were to evaluate the effect of surface roughness on the surface color and translucency of the composite resins. Two composite resins (Esthet-X, Dentsply, Milford, USA and Charisma, Kulzer, Domagen, Germany) were used to investigate the surface color. Charisma was used to investigate the translucency. 40 disc samples (diameter: 8 mm, thickness: 5 mm) were made by each product to measure the surface color. Polymerized each sample's one side was treated by Sof-Lex finishing and polishing system (Group C, M, F, SF). 40 disc samples (diameter: 6 mm, thickness: 1 mm) were prepared to measure the opacity. 1 mm samples were ground one side with #600, #1000, #1500 and #2000 sandpapers. CIE $L^{*}a^{*}b^{*}$ values of each 5 mm thickness samples, and XYZ values of 1 mm thickness samples on the white and black background were measured with spectrophotometer (Spectrolino, GretagMacbeth, Regensdorf, Switzerland). Mean surface roughness (Ra) of all samples before and after surface treatment was measured using the Surface Roughness Tester SJ-301 (Mytutoyo, Tokyo, Japan). Regardless of type and shade of the composite resin, $L^{*}$ values measured in group C were higher than others (p < 0.05), and $L^{*}$ value decreased as the Ra value decreased except B3 shade of Esthet-X. But there were no significant difference in $a^{*}$ values among groups. In control group and SF, highest $b^{*}$ values were measured (p < 0.05), except B1 shade of Esthet-X. Contrast ratio decreased as the Ra value decreased (p < 0.05). With the above results, difference of surface roughness has influence on surface color and translucency of dental composite resins.

• Applied Microscopy
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• v.37 no.2
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• pp.93-102
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• 2007

Secretory leukocyte protease inhibitor (SLPI) was known as one of bacterial lipopolysaccharide (LPS)-induced products of macrophage. Macrophages play an important role in the development of inflammatory responses by secreting an array of cytokines and chemokines in a tissue microenvironment. To identify the function and relationship between potent growth factors and SLPI after LPS stimulation, we conducted reverse transcriptase polymerase chain reaction (RT-PCR) and Western blots for the detection of mRNA and protein expression of SLPI and growth factors such as VEGF, PDGF, bFGF after 100 ng LPS stimulation on the RAW264.7 cells. The result of RT-PCR was showed SLPI mRNA expression was increased from 60 min to 48h in RAW 264.7 cells after incubation with LPS. VEGF and PDGF mRNA was expressed highly at initial stage by LPS stimulation. The mRNA of bFGF and type I collagen was very weakly expressed after LPS stimulation. SLPI protein level was increased likely the mRNA levels in RAW 267.7 cells. Additionally, phase contrast and scanning electron microscopic observation demonstrated that the LPS induce the change of morphology of the RAW264.7 cells. From these results, it suggest that expression of SLPI by LPS treatment may associate with VEGF and PDGF expression in RAW264.7 cells.

• Analytical Science and Technology
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• v.11 no.1
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• pp.62-72
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• 1998

This study was performed to characterize the trace organic pollutants in the industrial wastewater and to establish the database of the trace organic pollutants. The four manufacturing industries, which are refined petroleum, industrial chemicals, rubber & plastics and fabricated metals, were surveyed. The wastewater and discharging water of these 30 factories are analyzed to characterize the trace organic pollutants. In industrial chemicals, the kinds of products and organic pollutants are very various. Therefore to select the characteristic organic pollutants in this categories are also very difficult. In industrial chemicals, the gas chromatograpic peak patterns of wastewater are represented the various type according to their products, therefore the typical patterns of the characteristic organic pollutants could not be obtained because the kinds of manufactured goods and organic pollutants are very various. In refined petroleum, the effluent is discharged in the distillatory process of atmosphere pressure and contained the saturated hydrocarbons, phenol compounds, benzene compounds and naphtalene compounds. The saturated hydrocarbons peaks from $C_{15}$ to $C_{35}$ are represented the typical oil patterns by the uniform intervals therefore the peak can be easily distinguished. In rubber & plastics, the wastewater is discharged in the washing process which contains the additives. The problem of wastewater is not serious because the manufacturing process is not produced the effluent or the produced cooling water is recycled in that process.

• Journal of Animal Science and Technology
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• v.46 no.5
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• pp.773-782
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• 2004

The objective of this work was to establish an approaching method for TMR feeding system in the farm situation by testing the effects of herd-mix feeding system on the performance of lactating cows. Fifty six Holstein cows were fed for the experimental period of 16 months. Prior to test the herd-mix feeding system, animals were kept on conventional feeding system for 4 months, separate feeding of forage with concentrate, then provided 3 types of herd-mix rations formulated by mean level of energy concentration requirement of higher 1/2 ranker in each herd for remaining 12 months to compare the effect on milk yield performance of animals with conventional feeding system by using a switch-over method. The herd-mix feeding system influenced substantially upon the improvements of milk yield(P<0.01) and milk fat percentage(P<0.05). In all of lactational ages, milk yield and milk fat were enhanced by the herd-mix feeding system. Especially, actual milk yield(AMY) and milk fat in the 1st lactating cows, and AMY and 4.0% fat corrected milk yield(FCM) in the 2nd lactating cows were increased signiticantly by the herd-mix feeding system(P<0.05). In the early and mid-stage of lactation, the herd-mix feeding system showed higher AMY and FCM compared with the conventional feeding system although the milk fat was not different. AMY, milk fat and FCM in the late-stage of lactation were increased generally by the herd-mix feeding system. Especially, milk fat and FCM obtained by the herd-mix feeding system were significantly higher than those by the conventional feeding system(P<0.01). The herd-mix feeding system showed a good type of milking curve with a higher persistency of FCM(93.24%) than in conventional one(92.69%). The income over feed cost with the herd-mix feeding system was lA-fold higher than that with the conventional feeding method. In conclusion, the results of this work suggest that the herd-mix feeding system based on a correct level of energy concentration of TMR and well-determined feed ingredients increases not only milk yield of dairy herd by enhancing the performance of cows in early to mid stage of lactation with improved milk persistency but also gross income of dairy farm.

• Korean Journal of Veterinary Research
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• v.10 no.1
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• pp.11-23
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• 1970

Recently Carter(1952) reported the capsule antigens of Pasteurella multocida could be divided into four serological types A,B,C and D by means of precipitation tests. Subsequently he showed that the most sensitive for identification of these types involved the use of capsule substance adsorbed by erythrocytes in hemagglutination test. It may be somewhat difficult to conduct the hemagglutination test in small laboratory, because relatively large amounts of antisera and erythrocytes of the human O type are required for the test. A simple method for serological typing of P. multocida was the slide agglutination test employed by Little et al. (1943) and Namioka et al. (1962), but this method is still in controversy. The author tried adapting Carter's hemagglutination method to the slide method so called "micromethod technique", and studied on the stabilization of erythrocytes for use of slide hemagglutination to P. multocida although many invesigators reported the stabilization of erythrocytes. The results obtained are summarized as follows: 1. A simplified method (slide method) for capsule typing of the organism was developed by adapting Carter's hemagglutination reaction(tube method). Antibody-containing serum can be diluted serially on Boerner's microtest slide with capillary or serological pipetts with a considerable accuracy. The slide reaction can be carried out with case on the slide by adding $0.05m{\ell}$ of antigen-sensitized erythrocytes suspension diluted to one percent on $0.05m{\ell}$ of serially diluted antibody-containing sera, and the final result can be read after 60 minutes at the room temperature ($15^{\circ}C$). 2. It is difficult to determine superiority of inferiority between the slide method and the tube method on the pattern of the reaction of hemagglutination. 3. The pH range of 6.6 to 8.3 is optimal for the slide hemagglutination reaction. 4. The antigen-sensitization against erythrocytes at $37^{\circ}C$ is optimal for the slide hemagglutination. 5. Both the doses and concentration of antigen do not influence the antigen-adsorbing capacity of erythrocytes. 6. The reduction of antigen-sensitizing hours does not influence the antigen-adsorbing capacity of erythrocytes even 30 minutes. 7. The tannic acid treatment against formalinized and non-formalinized erythrocytes showed no effect on the reaction of hemagglutination. 8. The erythrocytes preserved at $4^{\circ}C$ in the ACD solution do not decrease the reactivity on the reaction of hemagglutination for 60 days, while they begin slight hemolysis 30 days after preserving. 9. The stable preparation of erythrocytes can be obtained by treating the cells at $37^{\circ}C$ for 20 hours with from 4 to 8 percent of formalin in saline or buffer. These cells can be preserved at $4^{\circ}C$ for more than 8 months experimented without hemolysis. With low concentration of formalin, the cells were not sufficiently stabilized resulting in the hemolysis after short period of preservation at $4^{\circ}C$. 10. The erythrocytes treated with 16 percent of formalin remain constantly or increase the reactivity for the reaction of hemagglutination. On the contrary, the cells treated with I to 8 percent of formalin decrease the reactivity. 11. There is no difference between nontreated fresh erythrocytes and the erythrocytes preserved in the ACD solution on the reactivity against the hemagglutination, and the erythrocytes treated with 16 percent of formalin showed the reactivity of higher level than that of the above two kinds of erythrocytes. 12. There is no difference between the saline and the isotonic buffer solution on the reaction of hemagglutination.

• Korean Journal of Weed Science
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• v.8 no.1
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• pp.53-58
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• 1988

This study was carried out to determine effect of butachlor [N.-(buthoxymethyl)-2-chloro-N-(2,6-diethylphenyl) acetamide] and bialaphos [2-amino-4(hydroxy)(methyl) phosphionyl] butyryl-alanylalanine sodium salt on the germination of tobacco seed, induction and growth of callus from tobacco. Further, fatty acids and ammonia content of tobacco calli were determined. Bialaphos had no effect on tobacco seed germination, but the growth of seedling was markedly affected by an application of 10 ppm bialaphos. However, regardless of varieties tested, tobacco seed germination was completely inhibited by $5{\times}10^{-5}M$ of butachlor. At an application of $5{\times}10^{-5}M$ butachlor, tobacco seeds were to some extent germinated and showed further growth. Hyangcho among varieties tested, showed the most tolerant response to butachlor. In induction of callus from various tobacco varieties and their growth, aromatic type of tobacco varieties exhibited the most tolerance against bialaphos. However, no distinct varietal differences were determined in the treatment of butachlor. The major fatty acids identified in tobacco calli were palmitic, oleic and linoleic acid. No marked difference in terms of fatty acids was observed among tobacco varieties used, but it was observed that there was the higher ratio of quantity in unsaturated fatty acids over saturated one, bialaphos treatment accumulated about 9 times higher ammonia content than that of the untreated control, giving an evidence that bialaphos might inhibit glutamine synthetase activity.

• Korean Journal of Weed Science
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• v.12 no.2
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• pp.144-157
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• 1992

The study was conducted to compare the interprete methods and examine the feasibility of mixture use of oxyfluorfen and bensulfuron in controlling principal Paddy weeds, annuals and perennials. Application ratio of both chemicals were obtained from the combinations of 5 levels(0, 5, 10, 15, 20 g ai/ha) of each chemicals, respectively. All the treatments were applied at 5 days after transplanting and water was maintained at 3.0cm in depth. Shoot fresh-weight of weeds was assessed at 35 days after treatments. Data obtained was analysed by Colby, Isobole, Calculus, Regression and EQM method, respectively. The results from the analysis of variance on the principal weeds treated with oxyfluorfen and bensulfuron showed significant interactions at 1% level on both Echinochloa crus-galli and Eleocharis Kuroguwai, and total species at 0.5% level on both Potamogeton distinctus and Cyperus serotinus, but non significant on Scirpus juncoides and Sagittaria pygmaea. Thereafter, the results of the models applied to Echinochloa crus-galli, Eleocharis kuroguwai and total species were as follows ; 1. The Colby method gave values nearly identical to regression estimate method (both multiplicative models) as provided by Akobundu et al. The Colby method and Regression method indicated synergistic toward Echinochloa curs-galli, and total species, but antagonistic toward Eleocharis kuroguwai. 2. The Isobole method shows synergism on Echinochloa crus-galli at $ID_{50}$, and total species at $ID_{60}$ on Eleochari kuroguwai. 3. The Calculus method gave positive signs for the first differentiation and negative signs for the second differentiation except for some rates on Echinochloa crus-galli and total species, but reverse on Eleocharis kuroguwai. These result does not agree with the observed values. 4. ${\theta}$ value from the EQM method was greater than one at all combinations. This result was quite different from those of other methods. 5. The various models did not show the same results, but mixture of oxyfluorfen and bensulfuron tend to have synergistic effect. Weeding effect also was high. Treatment in terms of two chemical combination was expected to reduce rates, and to enhence weeding efficacy compared with single treatment.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.28 no.2
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• pp.67-74
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• 2010

Law-protected trees are our precious asset as natural resources with history and tradition and natural heritage which should be protected and maintained well to bequeath next generation. Law-protected trees have not only thremmatologic and genetic meaning but also environmental and emotional meaning for their value to be high. This study investigated location, vitality, wrapping condition of root area and status of maintenance of the trees to figure out their growth environment and status of maintenance in a small-middle city through survey on those of law-protected trees in Je-cheon. There showed 300 more year old trees in Je-cheon mostly and the number of trees located in flat fields was the highest. For location type, village, hill and road types were presented in the order and for degree of development, land for building was found most frequently. The average electric resistance of the formative layer was measured to be $8.4k{\Omega}$ and four trees showed bark separation. Most law-protected trees underwent tree surgery, and complete bareness of root area was observed in a tree. The root area of two trees was covered with concrete. pH of soil was recorded to be 5.0~8.4 with its average of 7.1 and electric conductivity(EC) was less than 0.5 dS/m. For status of maintenance rearing facilities were placed for 16 trees out of totally 48 ones and stone fence was done for three ones. Tree surgery was conducted for 33 trees to prevent and to treat decomposed parts of holes. Direction boards were installed for 23 trees. Based on these results, measures to manage systematically law-protected trees in Jecheon could be suggested as follows. First, a sufficient space for growth of low part of trees should be secured. Second, a voluntary management should be induced by advertising them to residents in a community. Third, rearing facilities and direction boards of law-protected trees should be placed and related education should be conducted. Fourth, through operation of the department for law-protected trees consisting of related professions and cooperation among related departments the trees should be maintained continuously.