• Journal of The Korean Astronomical Society
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• v.41 no.5
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• pp.109-119
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• 2008

We present an optical-infrared photometric study of galaxies in six nearby clusters of galaxies at $z=0.041{\sim}0.098$ (A1436, A1773, A1809, A2048, A2142, and A2152). Using BV I photometry obtained at the Bohyunsan Optical Astronomical observatory and $JHK_S$ photometry extracted from the 2-Micron All-Sky Survey catalog, we investigate the colors of galaxies in the clusters. Using the (B - V) versus ($I\;-\;K_S$) color-color diagrams in comparison with the simple stellar population model, we estimate the ages and metallicities of bright early-type member galaxies. Early-type galaxies in each cluster show the color-magnitude relation. Ages and metallicities of early-type members show little dependence on their velocity dispersions. Mean ages of early-types in the clusters range from 3 Gyr to 20 Gyr, showing a large dispersion, and mean metallicities range from Z = 0.03 to 0.05 above the solar value, showing a negligible dispersion.

• Biomedical Science Letters
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• v.18 no.2
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• pp.152-159
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• 2012

Campylobacter species are known to the high optimum growth temperature ($42^{\circ}C$) and the cause of enteritis in people. Erythromycin has a curative effect for enteritis caused by the bacteria. However, the rate of erythromycin-resistant bacteria was not well known until recently in Korea. Swine are one of sources of the infection with a Campylobacter species which cause the symptom of a high temperature. In this study, we cultured rectum fecal specimens of 100 pigs in an area of Buan-gun, Jeonbuk Province during July 2009. As a result, the detection rate of C. jejuni and C. coli and the rate of erythromycin-resistant bacteria for the separated Campylobacter species on the condition of high temperature were investigated. The possession or not of hipO and glyA gene and ciprofloxacin-resistant gene gyrA was also reviewed with biochemical characteristics and PCR.

• Journal of Microbiology and Biotechnology
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• v.17 no.7
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• pp.1177-1182
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• 2007

Bacillus cereus group bacteria share a significant degree of genetic similarity. Thus, to differentiate and identify the Bacillus cereus group efficiently, a multiplex PCR method using the gyrB and groEL genes as diagnostic markers is suggested for simultaneous detection. The assay yielded a 400 bp amplicon for the groEL gene from all the B. cereus group bacteria, and a 253 bp amplicon from B. anthracis, 475 bp amplicon from B. cereus, 299 bp amplicon from B. thuringiensis, and 604 bp amplicon from B. mycoides for the gyrB gene. No nonspecific amplicons were observed with the DNA from 29 other pathogenic bacteria. The specificity and sensitivity of the B. cereus group identification using this multiplex PCR assay were evaluated with different kinds of food samples. In conclusion, the proposed multiplex PCR is a reliable, simple, rapid, and efficient method for the simultaneous identification of B. cereus group bacteria from food samples in a single tube.

• Archives of Pharmacal Research
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• v.21 no.6
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• pp.671-676
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• 1998

Five hundred and seventy clinical strains of Pseudomonas aeruginosa were isolated from August 1993 to August 1994 in Korea and screened for their resistance to ciprofloxacin, norfloxacin, and ofloxacin. Among these, two P. aeruginosa strains (PA150 and PA300) were selected based on their strong resistance (MICs > 50mcg/ml) to all three quinolones. The susceptible strain as well as two resistant strains had proton gradient-dependent efflux system. Efflux system in PA300 showed different specificities to ofloxacin and ciprofloxacin while PA150 had less permeability for ofloxacin. Ofloxacin had a less inhibitory action on DNA synthesis in permeabilized cells of PA150 and PA300 than 1771M. When quinolone resistance determining region (QRDR) in gyrA was sequenced, PA300 had one missense mutation, Asn 116Tyr, which was newly reported in this work. The results showed that PA150 became ofloxacin resistant by reduced ofloxacin accumulation due to the existence of efflux system and low permeability, while resistance of PA300 was due to the efflux system and a mutation in QRDR of gyrA -the target site of quinolone.

• The Plant Pathology Journal
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• v.33 no.1
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• pp.21-33
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• 2017

Citrus blast caused by bacterium Pseudomonas syringae is a very important disease of citrus occuring in many areas of the world, but with few data about genetic structure of the pathogen involved. Considering the above fact, this study reports genetic characterization of 43 P. syringae isolates obtained from plant tissue displaying citrus blast symptoms on mandarin (Citrus reticulata) in Montenegro, using multilocus sequence analysis of gyrB, rpoD, and gap1 gene sequences. Gene sequences from a collection of 54 reference pathotype strains of P. syringae from the Plant Associated and Environmental Microbes Database (PAMDB) was used to establish a genetic relationship with our isolates obtained from mandarin. Phylogenetic analyses of gyrB, rpoD, and gap1 gene sequences showed that P. syringae pv. syringae causes citrus blast in mandarin in Montenegro, and belongs to genomospecies 1. Genetic homogeneity of isolates suggested that the Montenegrian population might be clonal which indicates a possible common source of infection. These findings may assist in further epidemiological studies of this pathogen and for determining mandarin breeding strategies for P. syringae control.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.2
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• pp.33.1-33.1
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• 2015

M85 is a nearby merger remnant galaxy located at the northern part of the Virgo Cluster. Because of its remarkable merging features, it is an interesting object to investigate its formation history. Globular clusters are a great tracer of the formation history of early-type galaxies, so that we study the globular cluster system of M85. It has been already found that there are "intermediate-color" globular clusters as well as blue and red ones based on the photometric survey using CFHT/Megacam. For follow-up research, we obtain the spectra of 21 globular clusters in the central region of M85 using Gemini-N/GMOS. We estimate their ages and metallicities based on the strength of Lick indices. We detect the intermediate-age population (~ 2 Gyr) with solar metallicities, comprising about 50% of the observed globular clusters, as well as old and metal-poor population. It suggests that M85 experienced a major merging event around 2 Gyr ago. We discuss these results regarding to the formation history of M85.

• Korean Journal of Veterinary Service
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• v.35 no.3
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• pp.239-243
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• 2012

We reported an outbreak of Aeromonas (A.) veronii responsible for ulcerative dermatitis in Israeli carp (Cyprinus carpio). The major clinical signs were darkening body, abdominal extension, exophthalmos and severe ulcerative necrosis in the skin. The necropsy showed yellowish ascites, necrosis in liver and enlargement of kidney and spleen in the morbid fish. In blood agar for culturing bacteria, three different colonies were identified as A. veronii, Plesimonas shigelloides and Shewanella putrefaciens by phylogenetic identification using 16S rRNA or gyrB gene sequences. A. veronii was the most dominant species among them and was resistant to ampicillin, nalidixic acid and oxytetracycline.

• Journal of The Korean Astronomical Society
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• v.43 no.4
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• pp.105-113
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• 2010

We study the dynamical evolution of the M87 globular cluster (GC) system using the most advanced and realistic Fokker-Planck (FP) model.By comparing our FP models with both mass function (MF) and radial distribution (RD) of the observed GC system, we find the best-fit initial (at M87's age of 2-3 Gyr) MF and RD for three GC groups: all GCs, blue GCs, and red GCs. We estimate the initial total mass in GCs to be $1.8^{+0.3}_{-0.2}{\times}10^{10}M_{\bigodot}$, which is about 100 times larger than that of the Milky Way GC system. We also find that the fraction of the total mass currently in GCs is 34\%. When blue and red GCs are fitted separately, blue GCs initially have a larger total mass and a shallower radial distribution than red GCs. If one assumes that most of the significant major merger events of M87 have ended by the age of 2-3 Gyr, our finding that blue (metal-poor) GCs initially had a shallower radial distribution supports the major merger scenario for the origin of metallicity bimodality.

• The Plant Pathology Journal
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• v.36 no.3
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• pp.231-243
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• 2020

Rice blast, caused by Magnaporthe oryzae, is one of the most destructive rice diseases worldwide. The aim of this study was to screen bacterial isolates to efficiently prevent the occurrence of rice blast. A total of 232 bacterial isolates were extracted from nonrhizospheric rice soil and were screened for antifungal activity against M. oryzae using a leaf segment assay. Strains S170 and S9 showed significant antagonistic activity against M. oryzae in vitro and in leaf disk assays, and controlled M. oryzae infection under greenhouse conditions. The results showed that strains S170 and S9 could effectively control rice leaf blast and panicle neck blast after five spray treatments in field. This suggested that the bacterial strains S170 and S9 were valuable and promising for the biocontrol of rice disease caused by M. oryzae. Based on 16S rDNA, and gyrA and gyrB gene sequence analyses, S170 and S9 were identified as Bacillus amyloliquefaciens and B. pumilus, respectively. The research also demonstrated that B. amyloliquefaciens S170 and B. pumilus S9 could colonize rice plants to prevent pathogenic infection and evidently suppressed plant disease caused by 11 other plant pathogenic fungi. This is the first study to demonstrate that B. amyloliquefaciens and B. pumilus isolated from nonrhizospheric rice soil are capable of recolonizing internal rice stem tissues.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.1
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• pp.57.1-57.1
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• 2012

We present a spectroscopic study of the globular clusters (GCs) in the giant elliptical galaxy (gE) M86 in the Virgo galaxy cluster. Using the spectra obtained from the Multi-Object Spectroscopy (MOS) mode of Faint Object Camera and Spectrograph (FOCAS) on the Subaru Telescope, we measured the radial velocities of 25 GCs, the metallicities of 16 GCs, and the ages of 8 GCs in M86. The mean velocity and the vocity dispersion of the GCs are < ${\upsilon}_p$ > = -354 ${\pm}$ 80 km/s and ${\sigma}_p$ = 292 ${\pm}$ 32km/s, respectively. The M86 GC system shows some rotation with a large uncertainty and the velocity dispersion of the blue GC system is 60 km/s larger than that of the red GC system. The mean metallicity and age of M86 GCs are <[Fe/H]> = -1.13 ${\pm}$ 0.47 and = 9.7 ${\pm}$ 4.0 Gyr, respectively. We found one GC younger than 5 Gyr. We discuss the spectroscopic results of the M86 GC system in comparison with the GC systems in other gEs.